ASTM G160-12
(Practice)Standard Practice for Evaluating Microbial Susceptibility of Nonmetallic Materials By Laboratory Soil Burial
Standard Practice for Evaluating Microbial Susceptibility of Nonmetallic Materials By Laboratory Soil Burial
SIGNIFICANCE AND USE
3.1 These results may be used to compare the susceptibility of materials when exposed to this test procedure.
3.2 Microbiological susceptibility may be reflected by a number of changes including staining, weight loss, or reduction in tensile or flexural strength.
3.3 This practice may be considered an inoculation with a mixed culture of fungi and bacteria.
SCOPE
1.1 This practice is limited to the method of conducting an evaluation of a nonmetallic material's microbiological susceptibility when in contact with the natural environment of the soil under use conditions. This practice is intended for use on solid material test specimens that are no larger than approximately 2 cm (0.79 in.) thick and 100 cm 2 (15.5 in.2) or on film forming materials such as coatings which may be tested in the form of films at least 50 by 50 mm (2 by 2 in.) in size. This practice may be applied to articles that do not spend the majority of their service life in soil.
1.2 A wide variety of properties may be affected by microbial attack depending on material or item characteristics. Standard methods (where available) should be used for each different property to be evaluated. This practice does not attempt to enumerate all of the possible properties of interest nor specify the most appropriate test for those properties. Test methods must, however, be appropriate to the material being tested.
1.3 Materials intended for use in soil burial applications are often subjected to periods of exposure to solar radiation and other elements of weather for some time before they are buried. Because these exposures may alter the ability of a material to resist the effects of soil-borne microorganisms, it is recommended that this practice be combined with appropriate environmental exposures (for example, solar simulating weathering devices, the hydrolytic effects of extended aqueous contact, or extraneous nutrients) or fabrication into articles (for example, adhesive bonding of seams) which may promote microbiological susceptibility during the service life of the material.
1.4 The values stated in SI units are to be regarded as standard. The values given in parentheses are provided for information purposes only.
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
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Designation: G160 − 12
Standard Practice for
Evaluating Microbial Susceptibility of Nonmetallic Materials
1
By Laboratory Soil Burial
This standard is issued under the fixed designation G160; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope responsibility of the user of this standard to establish appro-
priate safety and health practices and determine the applica-
1.1 This practice is limited to the method of conducting an
bility of regulatory limitations prior to use.
evaluation of a nonmetallic material’s microbiological suscep-
tibilitywhenincontactwiththenaturalenvironmentofthesoil
2. Referenced Documents
under use conditions. This practice is intended for use on solid
2
2.1 ASTM Standards:
material test specimens that are no larger than approximately 2
2 2
G154 Practice for Operating Fluorescent Ultraviolet (UV)
cm (0.79 in.) thick and 100 cm (15.5 in. ) or on film forming
Lamp Apparatus for Exposure of Nonmetallic Materials
materials such as coatings which may be tested in the form of
G155 Practice for Operating XenonArc LightApparatus for
films at least 50 by 50 mm (2 by 2 in.) in size. This practice
Exposure of Non-Metallic Materials
may be applied to articles that do not spend the majority of
their service life in soil.
3. Significance and Use
1.2 A wide variety of properties may be affected by micro-
3.1 These results may be used to compare the susceptibility
bial attack depending on material or item characteristics.
of materials when exposed to this test procedure.
Standard methods (where available) should be used for each
3.2 Microbiological susceptibility may be reflected by a
different property to be evaluated. This practice does not
attempt to enumerate all of the possible properties of interest numberofchangesincludingstaining,weightloss,orreduction
in tensile or flexural strength.
nor specify the most appropriate test for those properties. Test
methods must, however, be appropriate to the material being
3.3 This practice may be considered an inoculation with a
tested.
mixed culture of fungi and bacteria.
1.3 Materials intended for use in soil burial applications are
4. Soil
often subjected to periods of exposure to solar radiation and
otherelementsofweatherforsometimebeforetheyareburied.
4.1 Composition— Soil shall be composed of equal parts of
Because these exposures may alter the ability of a material to
fertile topsoil (soil with a high clay content should not be
resist the effects of soil-borne microorganisms, it is recom-
used), well-rotted and shredded horse manure, and coarse sand
mended that this practice be combined with appropriate envi-
(10 to 40 mesh).
ronmentalexposures(forexample,solarsimulatingweathering
4.2 Mixing—Thesoilcompositionof4.1shouldbeprepared
devices, the hydrolytic effects of extended aqueous contact, or
1
by simple mixing and sifting through ⁄4-in. mesh screen.
extraneous nutrients) or fabrication into articles (for example,
4.3 Aging—The mixture is aged for three months and
adhesive bonding of seams) which may promote microbiologi-
resifted twice at four-week intervals during the three months.
cal susceptibility during the service life of the material.
Afterthreemonths,aviabilitycontrolofuntreatedcottoncloth,
1.4 The values stated in SI units are to be regarded as
2 2
400 to 475 g/m (12 to 14 oz/yd ), buried in the soil shall have
standard. The values given in parentheses are provided for
a tensile strength loss of at least 50 % after five days.
information purposes only.
NOTE 1—The soil mixture may be used for sequential tests as long as
the cotton cloth control degrades within the specified time period.
1.5 This standard does not purport to address all of the
safety concerns, if any, associated with its use. It is the
4.4 pH—The soil shall have a pH between 6.5 to 7.5,
checked periodically, and maintained by the addition of ground
1
This practice is under the jurisdiction of Committee G03 on Weathering and
Durability and is the direct responsibility of Subcommittee G03.04 on Biological
2
Deterioration. For referenced ASTM standards, visit the ASTM website, www.astm.org, or
Current edition approved Nov. 1, 2012. Published January 2013. Originally contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
approvedin1998.Lastpreviouseditionapprovedin2009asG160–03(2009).DOI: Standards volume information, refer to the standard’s Document Summary page on
10.1520/G0160-12. the ASTM website.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1
---------------------- Page: 1
...
This document is not an ASTM standard and is intended only to provide the user of an ASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
Designation: G160 − 03 (Reapproved 2009) G160 − 12
Standard Practice for
Evaluating Microbial Susceptibility of Nonmetallic Materials
1
By Laboratory Soil Burial
This standard is issued under the fixed designation G160; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope
1.1 This practice is limited to the method of conducting an evaluation of a nonmetallic material’s microbiological susceptibility
when in contact with the natural environment of the soil. soil under use conditions. This practice is intended for use on solid
2 2
3
material test specimens that are no larger than approximately 2 cm ((0.79 ⁄4 in.) thick and 100 cm (20(15.5 in. ) or on film forming
materials such as coatings which may be tested in the form of films at least 50 by 50 mm (2 by 2 in.) in size. This practice may
be applied to articles that do not spend the majority of their service life in soil.
1.2 A wide variety of properties may be affected by microbial attack depending on material or item characteristics. Standard
methods (where available) should be used for each different property to be evaluated. This practice does not attempt to enumerate
all of the possible properties of interest nor specify the most appropriate test for those properties. Test methods must, however, be
appropriate to the material being tested.
1.3 It Materials intended for use in soil burial applications are often subjected to periods of exposure to solar radiation and other
elements of weather for some time before they are buried. Because these exposures may alter the ability of a material to resist the
effects of soil-borne microorganisms, it is recommended that this practice be combined with appropriate environmental exposures
(for example, sunlightsolar simulating weathering devices, the hydrolytic effects of extended aqueous contact, or extraneous
nutrients) or fabrication into articles (for example, adhesive bonding of seams) which may promote microbiological susceptibility
during the service life of the material.
1.4 The values stated in SI units are to be regarded as standard. The values given in parentheses are provided for information
purposes only.
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility
of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory
limitations prior to use.
2. Referenced Documents
2
2.1 ASTM Standards:
G154 Practice for Operating Fluorescent Ultraviolet (UV) Lamp Apparatus for Exposure of Nonmetallic Materials
G155 Practice for Operating Xenon Arc Light Apparatus for Exposure of Non-Metallic Materials
3. Significance and Use
3.1 These results may be used to compare the susceptibility of materials when exposed to this test procedure.
3.2 Microbiological susceptibility may be reflected by a number of changes including staining, weight loss, or reduction in
tensile or flexural strength.
3.3 This practice may be considered an inoculation with a mixed culture of fungi and bacteria.
4. Soil
4.1 Composition— Soil shall be composed of equal parts of fertile topsoil (soil with a high clay content should not be used),
well-rotted and shredded horse manure, and coarse sand (10 to 40 mesh).
1
This practice is under the jurisdiction of Committee G03 on Weathering and Durability and is the direct responsibility of Subcommittee G03.04 on Biological
Deterioration.
Current edition approved June 1, 2009Nov. 1, 2012. Published July 2009 January 2013. Originally approved in 1998. Last previous edition approved in 20032009 as G160
– 03.03(2009). DOI: 10.1520/G0160-03R09.10.1520/G0160-12.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM Standards
volume information, refer to the standard’s Document Summary page on the ASTM website.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1
---------------------- Page: 1 ----------------------
G160 − 12
1
4.2 Mixing—The soil composition of 3.14.1 should be prepared by simple mixing and sifting through ⁄4-in. mesh screen.
4.3 Aging—The mixture is aged for three months and resifted twice at four-week interva
...
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