ASTM D4200-82(2019)
(Test Method)Standard Test Method for Evaluating Inhibitory Effects of Ink Grids on Membrane Filters
Standard Test Method for Evaluating Inhibitory Effects of Ink Grids on Membrane Filters
SIGNIFICANCE AND USE
5.1 This test method may be applied to determine the suitability of grid-marked membrane filters for use in bacteriological culture techniques for the detection and enumeration of bacterial organisms.
5.2 A particularly sensitive organism and growth conditions have been selected for this test method in order to maximize sensitivity to toxic materials possibly present in the inks used for grid-marking membrane filters.
SCOPE
1.1 This test method describes a procedure whereby the user of ink-gridded membrane filters in water quality studies can ascertain whether or not the grid lines are toxic and inhibitory to bacterial growth when the membrane and its entrapped bacteria are incubated on a suitable media.
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
General Information
Relations
Standards Content (Sample)
This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the
Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
Designation: D4200 − 82 (Reapproved 2019)
Standard Test Method for
Evaluating Inhibitory Effects of Ink Grids on Membrane
Filters
This standard is issued under the fixed designation D4200; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 4. Summary of Test Method
4.1 A heavy bacterial suspension is filtered through a
1.1 Thistestmethoddescribesaprocedurewherebytheuser
of ink-gridded membrane filters in water quality studies can gridded membrane filter.The bacterial concentration employed
is sufficient to cover much of the membrane with bacterial
ascertain whether or not the grid lines are toxic and inhibitory
to bacterial growth when the membrane and its entrapped colonies.
bacteria are incubated on a suitable media.
4.2 After filtration the membrane is incubated on a suitable
medium and the distribution of the colonies and shape of the
1.2 The values stated in SI units are to be regarded as
colonies noted in the area around each grid line.
standard. No other units of measurement are included in this
standard.
5. Significance and Use
1.3 This standard does not purport to address all of the
safety concerns, if any, associated with its use. It is the 5.1 This test method may be applied to determine the
responsibility of the user of this standard to establish appro- suitability of grid-marked membrane filters for use in bacte-
priate safety, health, and environmental practices and deter- riological culture techniques for the detection and enumeration
mine the applicability of regulatory limitations prior to use. of bacterial organisms.
1.4 This international standard was developed in accor-
5.2 Aparticularly sensitive organism and growth conditions
dance with internationally recognized principles on standard-
have been selected for this test method in order to maximize
ization established in the Decision on Principles for the
sensitivity to toxic materials possibly present in the inks used
Development of International Standards, Guides and Recom-
for grid-marking membrane filters.
mendations issued by the World Trade Organization Technical
Barriers to Trade (TBT) Committee.
6. Apparatus
6.1 Incubator, capable of maintaining temperatures of
2. Referenced Documents
44.5 6 0.2°C.
2.1 ASTM Standards:
6.2 Membrane Filtration Units.
D1129 Terminology Relating to Water
D1193 Specification for Reagent Water
6.3 Vacuum Source with trap vessel.
6.4 Forceps, blunt-nosed.
3. Terminology
6.5 Autoclave or other sterilizing equipment.
3.1 Definitions:
6.6 Expendables:
3.1.1 For definitions of terms used in this standard, refer to
6.6.1 Gridded membrane filters.
Terminology D1129.
6.6.2 1-mL and 10-mL pipets.
6.6.3 Petri dishes (50-mm) containing 6 to 8 mL of agar
medium or a 100-mm dish with 20 6 2 mLof agar medium, or
This test method is under the jurisdiction of ASTM Committee D19 on Water
both.
and is the direct responsibility of Subcommittee D19.08 on Membranes and Ion
Exchange Materials. 6.6.4 Erlenmeyer flasks.
Current edition approved Nov. 1, 2019. Published January 2020. Originally
approved in 1982. Last previous edition approved in 2011 as D4200 – 82 (2011).
7. Reagents and Materials
DOI: 10.1520/D4200-82R19.
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
7.1 Purity of Water—Unless otherwise indicated, reference
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
to water shall be understood to mean reagent water confor
...
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