Standard Specification for Pipet, Blood Diluting

ABSTRACT
This specification covers the requirements for glass reusable blood diluting pipets that are used for performing red and white cell corpuscle determinations. The pipets shall be made of common spirit bore white back tubing or clear glass with a white stripe applied to the outer surface of the tubing. The beads shall be made of glass or ceramic composition and shall be of red, clear, or white coloring. Materials shall be tested and the individual types shall conform to specified values of design, capacity, capacity markings, graduation lines, accuracy, workmanship, identification, and pigmentation.
SCOPE
1.1 This specification covers requirements for glass reusable blood diluting pipets that are used for performing red and white cell corpuscle determinations.

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31-Oct-2008
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ASTM E788-97(2008) - Standard Specification for Pipet, Blood Diluting
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NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation:E788 −97(Reapproved 2008)
Standard Specification for
Pipet, Blood Diluting
This standard is issued under the fixed designation E788; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
This standard has been approved for use by agencies of the Department of Defense.
1. Scope 5. Dimensions and Permissible Variations
1.1 Thisspecificationcoversrequirementsforglassreusable 5.1 Design—The red and white cell blood dilution pipets
blooddilutingpipetsthatareusedforperformingredandwhite shall consist of a small uniform bore glass tube which shall
cell corpuscle determinations. have a bulb of proper size near the proximal end (see Fig. 1).
As an alternative, the pipets may be constructed of three pieces
2. Referenced Documents of glass that are fused together to form a one-piece pipet (see
Fig. 2). The bulb shall contain a nonspherical glass or ceramic
2.1 ASTM Standards:
bead sufficiently large enough to prevent its being impacted in
E694 Specification for Laboratory Glass Volumetric Appa-
the constriction portion of the bulb. The cross section of the
ratus
pipet at any point shall be circular. The distal end of the white
E920 Specification for Commercially Packaged Laboratory
cell pipet shall be pulled to a point and then ground and
Apparatus
polished to a tapered tip. The distal end of the red cell pipet
E921 Specification for Export Packaged Laboratory Appa-
shall be ground and polished to a tapered tip. The inside
ratus
diameter (ID) of the pipet bore at the tip end of the white cell
E1133 Practice for Performance Testing of Packaged Labo-
pipet shall be 0.2 to 0.5 mm. The external diameter of the
ratory Apparatus for United States Government Procure-
ground and polished tip shall not exceed 2.0 mm. The overall
ments
lengthofbothpipetsshallbe104to121mm.Theproximalend
E1157 Specification for Sampling and Testing of Reusable
may be of funnel design, with an exterior taper, or ground and
Laboratory Glassware
polishedwithataperofsufficientangletopermitapplicationof
a rubber suction tube or other suction device (see Fig. 2).
3. Classification
5.2 Capacity—The capacity of the red cell pipet bulb shall
3.1 This specification covers two different pipet designs and
be 0.8 to 1.2 cm and the capacity of the white cell bulb shall be
permissible alternatives.
0.2 to 0.4 cm . The capacity of the red cell pipet stem (bulb to
3.1.1 Red and white cell blood diluting pipets (see Fig. 1).
distal end) shall be 0.008 to 0.012 cm and the capacity of the
3.1.2 Permissible alternative designs (see Fig. 2).
white cell pipet stem shall be 0.02 to 0.04 cm .
4. Materials 5.3 Capacity Markings:
5.3.1 Pipet Bulb Markings—The red and white cell pipet
4.1 The pipets shall be made of common spirit bore white
bulb capacity shall be confined to and indicated by calibration
back tubing or clear glass with a white stripe applied to the
lines on the pipet. These lines shall be located on both sides of
outer surface of the tubing.
the bulb and shall be within 2 mm to 6 mm above the bulb
4.2 The beads shall be made of glass or ceramic composi-
(proximalend)andwithin3mmto6mmbelowthebulb(distal
tion and shall be of red, clear, or white coloring.
end). The exact manner for measuring these line placements is
specified in Fig. 1.
5.3.2 Pipet Stem Markings—The graduation lines on the
This specification is under the jurisdiction of ASTM Committee E41 on
stem of the red and white cell pipets shall be equally divided
Laboratory Apparatusand is the direct responsibility of Subcommittee E41.01 on
into two or ten divisions. If there are two divisions, the two
Apparatus.
calibration lines on the stem will be numbered 0.5 and 1. If
Current edition approved Nov. 1, 2008. Published January 2009. Originally
there are ten divisions, the ten calibration lines will be
approvedin1981.Lastpreviouseditionapprovedin2003asE788 – 97(2003).DOI:
10.1520/E0788-97R08.
numbered 0.5 on the fifth line and 1 on the tenth line
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
respectively. The top line (above bulb at the proximal end)
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
shall be numbered 101 on the red cell pipet and 11 on the white
Standards volume information, refer to the standard’s Document Summary page on
the ASTM website. cell pipet, representing stem and bulb volume collectively. At
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
E788−97 (2008)
FIG. 1 Pipet Dimensions
the option of the manufacturer, pipet stems may be graduated volumetric tolerances on the pipet. These tolerances may be
with split lines or partial length lines (see Fig. 2). located on the stem or the proximal end of the pipet.
5.7.1 The white cell pipet shall have a clear or white glass
5.4 Graduation Lines—All graduation lines on the red and
or ceramic bead sealed within the bulb to identify it readily as
white cell
...

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