ASTM E1292-94(2011)
(Specification)Standard Specification for Gravity Convection and Forced Ventilation Incubators
Standard Specification for Gravity Convection and Forced Ventilation Incubators
ABSTRACT
This specification covers the performance requirements for general-purpose air incubators ordinarily used for incubating procedures. It is applicable to gravity and forced ventilation incubators designed to operate over a specified range of temperature. This specification does not include any requirements for the safe handling of harmful or disease bearing organisms. Temperature uniformity within a specified period of time shall be tested using several thermocouples.
SCOPE
1.1 This specification covers the performance requirements for general purpose air incubators ordinarily used for incubating procedures, which have an incubating chamber up to 0.6 m3 (25 ft3) in volume. It is applicable to gravity and forced ventilation incubators designed to operate over all or part of the temperature range from 5°C above ambient to 75°C.
1.2 This specification does not include any requirements for the safe handling of harmful or disease bearing organisms.
The following precautionary caveat pertains only to the test method portions, Sections 4, 5, and 6, of this specification. This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
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Designation:E1292 −94(Reapproved 2011)
Standard Specification for
Gravity Convection and Forced Ventilation Incubators
This standard is issued under the fixed designation E1292; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 3.2 The rate of ventilation of the incubating chamber shall
conform to the requirements specified in Table 1 for the
1.1 This specification covers the performance requirements
particular type of incubator when measured in accordance with
for general purpose air incubators ordinarily used for incubat-
the procedure given in Section 5.
ing procedures, which have an incubating chamber up to 0.6
3 3
m (25 ft ) in volume. It is applicable to gravity and forced
3.3 The recovery time of the incubating chamber shall
ventilationincubatorsdesignedtooperateoverallorpartofthe conform to the requirements specified in Table 1 for the
temperature range from 5°C above ambient to 75°C.
particulartypeofincubator,whentestedinaccordancewiththe
procedure specified in Section 6.
1.2 This specification does not include any requirements for
the safe handling of harmful or disease bearing organisms.
4. Test Methods
1.3 The following precautionary caveat pertains only to the
4.1 Temperature Uniformity:
test method portions, Sections 4, 5, and 6, of this specification.
4.1.1 Place nine calibrated thermocouples (Note 1) made
This standard does not purport to address all of the safety
from iron-constantan or copper-constantan wires approxi-
concerns, if any, associated with its use. It is the responsibility
mately 0.5 mm in diameter (No. 24 gauge) and having a
of the user of this standard to establish appropriate safety and
junction size of not more than 2 mm (0.08 in.) in the empty
health practices and determine the applicability of regulatory
incubating chamber with shelves in place, and vents open.
limitations prior to use.
Locate one thermocouple in each of the eight corners of the
incubator approximately 5 cm (2 in.) from each wall, and place
2. Types
the ninth thermocouple within 2.5 cm (1 in.) of the geometric
2.1 This specification covers the following four types of air
center of the chamber. A minimum length of 30 cm (12 in.) of
incubators:
wire for each thermocouple shall be inside the incubator to
2.1.1 Type 1A—An incubator ventilated by gravity convec-
minimize the conduction of heat from the thermocouple.
tion having a uniformity of temperature within 3 % of the
differential between incubator and ambient temperature, NOTE 1—If calibrated thermocouples are not available, nine thermo-
couples made from the same spool of wire may be used, provided they
2.1.2 Type 1B—An incubator ventilated by gravity convec-
give the same value for temperature when placed adjacent to one another
tion having a uniformity of temperature within 6 % of the
in the incubating chamber at the temperature of the test.
differential between incubator and ambient temperature,
4.1.2 Bring the incubator to the specified temperature and
2.1.3 Type 2A—An incubator ventilated by forced ventila-
allow it to reach a steady state (Note 2). Record the tempera-
tion having a uniformity of temperature within 2 % of the
ture of the nine thermocouples for a period of at least 12 h and
differential between incubator and ambient temperature,
determine from the record the maximum deviation of each
2.1.4 Type 2B—An incubator ventilated by forced ventila-
point from the desired temperature. The ambient room tem-
tion having a uniformity of temperature within 5 % of the
perature shall vary by not more than a total of 5°C and the line
differential between incubator and ambient temperature.
voltage shall not vary by more than a total of 5 % during the
3. Performance Requirements
test.
3.1 The temperature within the incubating chamber shall be
NOTE 2—Some incubators may require as much as 12 h to reach a
controlled by an automatic device, and shall be uniform within
steady state. If a steady state does not exist, there is a drift in the
the tolerances given in Table 1, when tested in accordance with
temperature toward the steady state condition.
Section 4.
5. Rate of Ventilation
5.1 Seal the ventilation ports, doors, and all apertures of the
This specification is under the jurisdiction of ASTM Committee E41 on
Laboratory Apparatus and is the direct responsibility of Subcommittee E
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