ASTM E979-09
(Test Method)Standard Test Method for Evaluation of Antimicrobial Agents as Preservatives for Invert Emulsion and Other Water Containing Hydraulic Fluids
Standard Test Method for Evaluation of Antimicrobial Agents as Preservatives for Invert Emulsion and Other Water Containing Hydraulic Fluids
SIGNIFICANCE AND USE
This procedure is designed to determine the effectiveness of antimicrobial agents intended for microbial control in invert emulsions and other water containing hydraulic fluids.
SCOPE
1.1 This laboratory practice is designed to evaluate the utility and effectiveness of antimicrobial agents intended to control microbial growth in invert emulsions and other water containing hydraulic fluids.
Note 1—Procedures for preparation of water soluble hydraulic fluids and recovery of organisms appear in Practice E 2169.
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
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Designation: E979 − 09
StandardPractice for
Evaluation of Antimicrobial Agents as Preservatives for
Invert Emulsion and Other Water Containing Hydraulic
1
Fluids
This standard is issued under the fixed designation E979; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
INTRODUCTION
Invert emulsion hydraulic fluids typically contain 60% mineral oil and 40% water (by volume).
These fluids routinely are prepared using proprietary, oil-soluble, emulsifying agents, as well as other
emulsifiable constituents. They are recommended for use where conditions indicate a low-cost, fire
retardant product, compatible with water-based metal working fluids.
The high water content of these hydraulic fluids makes them susceptible to microbial attack.
Uncontrolled microbial growth in these fluids can cause cartridge filter unit plugging, maladorous
conditions, or general biodeterioration. Problem microorganisms associated with these fluids include
bacteria and fungi.
Thehydraulicsystemisessentiallyaclosedoneinwhichwaterofevaporationisaddedtomaintain
a fixed volume. The inclusion of an efficacious preservative in the water containing hydraulic fluids
can prevent microbial growth and the resulting problems that follow.
1. Scope* 2. Referenced Documents
2
1.1 This laboratory practice is designed to evaluate the 2.1 ASTM Standards:
utility and effectiveness of antimicrobial agents intended to D1129Terminology Relating to Water
control microbial growth in invert emulsions and other water D4454Test Method for Simultaneous Enumeration of Total
containing hydraulic fluids. andRespiringBacteriainAquaticSystemsbyMicroscopy
D5465Practice for Determining Microbial Colony Counts
NOTE 1—Procedures for preparation of water soluble hydraulic fluids
from Waters Analyzed by Plating Methods
and recovery of organisms appear in Practice E2169.
E1326Guide for Evaluating Non-culture Microbiological
1.2 The values stated in SI units are to be regarded as
Tests Used for Enumerating Bacteria
standard. No other units of measurement are included in this
E2169Practice for Selecting Antimicrobial Pesticides for
standard.
Use in Water-Miscible Metalworking Fluids
1.3 This standard does not purport to address all of the
E2523Terminology for Metalworking Fluids and Opera-
safety concerns, if any, associated with its use. It is the
tions
responsibility of the user of this standard to establish appro-
3. Terminology
priate safety and health practices and determine the applica-
bility of regulatory limitations prior to use.
3.1 Terms used in this practice are defined inTerminologies
D1129 and E2523.
1
This practice is under the jurisdiction ofASTM Committee E35 on Pesticides,
Antimicrobials, and Alternative Control Agents and is the direct responsibility of
2
Subcommittee E35.15 on Antimicrobial Agents. For referenced ASTM standards, visit the ASTM website, www.astm.org, or
Current edition approved . Published April 2009. Originally approved in 1984. contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
Last previous edition approved in 2004 as E979–91(2004). DOI: 10.1520/E0979- Standards volume information, refer to the standard’s Document Summary page on
09. the ASTM website.
*A Summary of Changes section appears at the end of this standard
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
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E979 − 09
1
4. Summary of Test Method 6.6.1 Strainer, 3-in. epoxy coated, ⁄4-in. mesh gutter
3
strainer.
4.1 The antimicrobial agent to be evaluated is incorporated
6.6.2 Screen, 16 by 18 in. fiberglass screening material.
into an emulsion system by (a) addition to the aqueous phase
employedinthepreparationoftheemulsion,(b)indosestothe NOTE 4—Fiberglass mesh screening material (16 by 18 in.) is available
from any local hardware dealer.
formulatedsystem,or(c)byothermethodssuitableforthetest
compound.
6.6.3 Wire, 20-gage, galvanized or stainless steel.
1
4.2 A heavy bacterial or fungal inoculum, or both, is then 6.7 Tubing, ⁄4-in. ID Tygon.
added.
NOTE 5—Tygon is available from most local laboratory supply houses.
1
4.3 The resulting mixture is aerated and passed over the
6.8 T-Connectors, ⁄4-in. polypropylene.
surface of a simulated filter system for a minimum period of
6.9 Laboratory Blender—Any standard adjustable speed
eight weeks either continuously or with shutdowns to simulate
laboratory blender having a 2-L capacity glass or metal
actual operations conditions.
container is satisfactory.
4.4 The degree of microbial control is determined
...
This document is not anASTM standard and is intended only to provide the user of anASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
Designation:E979–91(Reapproved 2004)
Standard Test Method for Designation:E979–09
Standard Practice for
Evaluation of Antimicrobial Agents as Preservatives for
Invert Emulsion and Other Water Containing Hydraulic
1
Fluids
This standard is issued under the fixed designation E979; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
INTRODUCTION
Invert emulsion hydraulic fluids typically contain 60% mineral oil and 40% water (by volume).
These fluids routinely are prepared using proprietary, oil-soluble, emulsifying agents, as well as other
emulsifiable constituents. They are recommended for use where conditions indicate a low-cost, fire
retardant product, compatible with water-based metal working fluids.
The high water content of these hydraulic fluids makes them susceptible to microbial attack.
Uncontrolled microbial growth in these fluids can cause cartridge filter unit plugging, maladorous
conditions, or general biodeterioration. Problem microorganisms associated with these fluids include
bacteria and fungi.
Thehydraulicsystemisessentiallyaclosedoneinwhichwaterofevaporationisaddedtomaintain
a fixed volume. The inclusion of an efficacious preservative in the water containing hydraulic fluids
can prevent microbial growth and the resulting problems that follow.
1. Scope
1.1This laboratory test method is designed to evaluate the utility and effectiveness of antimicrobial agents intended to control
microbial growth in invert emulsions and other water containing hydraulic fluids. *
1.1 This laboratory practice is designed to evaluate the utility and effectiveness of antimicrobial agents intended to control
microbial growth in invert emulsions and other water containing hydraulic fluids.
NOTE 1—Procedures for preparation of water soluble hydraulic fluids and recovery of organisms appear in Method E686Practice E2169.
1.2
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility
of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory
limitations prior to use.
2. Referenced Documents
2
2.1 ASTM Standards: ASTM Standards:
D1129 Terminology Relating to Water
D4454 Test Method for Simultaneous Enumeration of Total and Respiring Bacteria in Aquatic Systems by Microscopy
D5465 Practice for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods
E686Method for Evaluation of Antimicrobial Agents in Aqueous Metal Working Fluids 1326 Guide for Evaluating
Nonconventional Microbiological Tests Used for Enumerating Bacteria
1
ThistestmethodpracticeisunderthejurisdictionofASTMCommitteeE35onPesticidesandAlternativeControlAgentsandisthedirectresponsibilityofSubcommittee
E35.15 on Antimicrobial Agents.
Current edition approved April 1, 2004.2009. Published May 2004.April 2009. Originally approved in 1984. Last previous edition approved in 19982004as
E979–91(1998).E979–91(2004).
2
ForreferencedASTMstandards,visittheASTMwebsite,www.astm.org,orcontactASTMCustomerServiceatservice@astm.org.For Annual Book of ASTM Standards
volume information, refer to the standard’s Document Summary page on the ASTM website.
*A Summary of Changes section appears at the end of this standard.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.
1
---------------------- Page: 1 ----------------------
E979–09
E2169 Practice for Selecting Antimicrobial Pesticides for Use in Water-Miscible Metalworking Fluids
E2523 Terminology for Metalworking Fluids and Operations
3. Terminology
3.1 Terms used in this practice are defined in Terminologies D1129 and E2523.
4. Summary of Test Method
3.1The4.1 Theantimicrobialagenttobeevaluatedisincorporatedintoanemulsionsystemby(a)additiontotheaqueousphase
employed in the preparation of the emulsion, (b) in doses to the formulated system, or (c) by other methods suitable for the test
compound.
3.2A heavy bacterial or fungal inoculum, or both, is then added.
3.3Theresultingmixtureisaeratedandpassedoverthesurfaceofasimulatedfiltersystemforaminimumperiodofeightweeks
either continuously or with shutdowns to simulate ac
...
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