ASTM D7788-14(2023)
(Practice)Standard Practice for Collection of Total Airborne Fungal Structures via Inertial Impaction Methodology
Standard Practice for Collection of Total Airborne Fungal Structures via Inertial Impaction Methodology
SIGNIFICANCE AND USE
4.1 This practice is intended for the collection of airborne fungal spores or fragments, or both, using inertial impaction.
4.2 It is the responsibility of the user to assure that they are in compliance with all local, state and federal regulations governing the inspection of buildings for fungal colonization and the collection of associated samples.
4.3 This practice is intended to provide the user with a basic understanding of the equipment, materials and instructions necessary to effectively collect air samples using an inertial impactor.
4.4 This practice, when properly executed, may also be used for the evaluation of other types of airborne particles with the capturing characteristics appropriate for inertial impactor, and for which appropriate analytical methods exist. Such particles may include dust mites, skin cells, pollen, and other materials.
SCOPE
1.1 The purpose of this practice is to describe procedures for the collection of airborne fungal spores or fragments, or both, using inertial impaction sampling techniques.
1.2 This practice is not intended to limit the user from the collection of other airborne particulates that may be of interest and captured through this technique.
1.3 This practice presumes that the user has a fundamental understanding of field investigative techniques related to the scientific process, and sampling plan development and implementation. It is important to establish the related hypothesis to be tested and the supporting analytical methodology needed in order to identify the sampling media to be used and the laboratory conditions for analysis.
1.4 This practice does not address the development of a formal hypothesis or the establishment of appropriate and defensible investigation and sampling objectives. It is presumed the investigator has the experience and knowledge base to address these issues.
1.5 This practice does not provide the user sufficient information to allow for interpretation of the analytical results from sample collection. It is the user's responsibility to seek or obtain the information and knowledge necessary to interpret the sample results reported by the laboratory.
1.6 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.
1.8 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
General Information
Relations
Standards Content (Sample)
This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the
Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
Designation: D7788 − 14 (Reapproved 2023)
Standard Practice for
Collection of Total Airborne Fungal Structures via Inertial
1
Impaction Methodology
This standard is issued under the fixed designation D7788; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope ization established in the Decision on Principles for the
Development of International Standards, Guides and Recom-
1.1 The purpose of this practice is to describe procedures for
mendations issued by the World Trade Organization Technical
the collection of airborne fungal spores or fragments, or both,
Barriers to Trade (TBT) Committee.
using inertial impaction sampling techniques.
1.2 This practice is not intended to limit the user from the
2. Referenced Documents
collection of other airborne particulates that may be of interest
2
2.1 ASTM Standards:
and captured through this technique.
D1356 Terminology Relating to Sampling and Analysis of
1.3 This practice presumes that the user has a fundamental
Atmospheres
understanding of field investigative techniques related to the
D3195/D3195M Practice for Rotameter Calibration
scientific process, and sampling plan development and imple-
D4840 Guide for Sample Chain-of-Custody Procedures
mentation. It is important to establish the related hypothesis to
D6044 Guide for Representative Sampling for Management
be tested and the supporting analytical methodology needed in
of Waste and Contaminated Media
order to identify the sampling media to be used and the
D7391 Test Method for Categorization and Quantification of
laboratory conditions for analysis.
Airborne Fungal Structures in an Inertial Impaction
Sample by Optical Microscopy
1.4 This practice does not address the development of a
formal hypothesis or the establishment of appropriate and
3. Terminology
defensible investigation and sampling objectives. It is pre-
sumed the investigator has the experience and knowledge base
3.1 Definitions—For definitions and terms not listed here,
to address these issues.
see Terminology D1356.
1.5 This practice does not provide the user sufficient infor- 3.1.1 inertial impactor, n—a device designed for the impac-
mation to allow for interpretation of the analytical results from tion of particles that are separated from the air stream by inertia
sample collection. It is the user’s responsibility to seek or onto a collection surface. D7391
obtain the information and knowledge necessary to interpret 3.1.1.1 Discussion—Inertial impactors are available in
the sample results reported by the laboratory. many designs including “slit” and “circular” jets.
3.1.1.2 Discussion—Allows for the identification to genus
1.6 The values stated in SI units are to be regarded as
3
or group of fungi detected, quantification to spores/m , and
standard. No other units of measurement are included in this
general assessment of background debris. Identification of
standard.
pollen, hyphal fragments and other airborne particulate may be
1.7 This standard does not purport to address all of the
included.
safety concerns, if any, associated with its use. It is the
3.1.2 sample, n—a portion of a population. A portion of
responsibility of the user of this standard to establish appro-
material that is taken for testing or record purposes. D6044
priate safety, health, and environmental practices and deter-
3.1.3 sample, representative, n—a sample collected in such
mine the applicability of regulatory limitations prior to use.
a manner that it reflects one or more characteristics of interest
1.8 This international standard was developed in accor-
(as defined by the project objectives) of a population from
dance with internationally recognized principles on standard-
which it is collected. D6044
1
This practice is under the jurisdiction of ASTM Committee D22 on Air Quality
and is the direct responsibility of Subcommittee D22.08 on Assessment, Sampling,
2
and Analysis of Microorganisms. For referenced ASTM standards, visit the ASTM website, www.astm.org, or
Current edition approved Jan. 1, 2023. Published February 2023. Originally contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
approved in 2014. Last previous edition approved in 2014 as D7788 – 14. DOI: Standards volume information, refer to the standard’s Document Summary page on
10.1520/D7788-14R23. the ASTM website.
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