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ASTM E169-16(2022)

(Practice)

Standard Practices for General Techniques of Ultraviolet-Visible Quantitative Analysis

Standard Practices for General Techniques of Ultraviolet-Visible Quantitative Analysis

SIGNIFICANCE AND USE
4.1 These practices are a source of general information on the techniques of ultraviolet and visible quantitative analyses. They provide the user with background information that should help ensure the reliability of spectrophotometric measurements.  
4.2 These practices are not intended as a substitute for a thorough understanding of any particular analytical method. It is the responsibility of the users to familiarize themselves with the critical details of a method and the proper operation of the available instrumentation.
SCOPE
1.1 These practices are intended to provide general information on the techniques most often used in ultraviolet and visible quantitative analysis. The purpose is to render unnecessary the repetition of these descriptions of techniques in individual methods for quantitative analysis.  
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

General Information

Status
Published
Publication Date
31-Oct-2022
ICS
71.040.50 - Physicochemical methods of analysis
Technical Committee
E13 - Molecular Spectroscopy and Separation Science
Drafting Committee
E13.01 - Ultra-Violet, Visible, and Luminescence Spectroscopy
Current Stage
Ref Project

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ASTM E169-16(2022) - Standard Practices for General Techniques of Ultraviolet-Visible Quantitative AnalysisASTM E169-16(2022) - Standard Practices for General Techniques of Ultraviolet-Visible Quantitative Analysis
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ASTM E169-16(2022) - Standard Practices for General Techniques of Ultraviolet-Visible Quantitative Analysis
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This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the
Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
Designation: E169 − 16 (Reapproved 2022)
Standard Practices for
General Techniques of Ultraviolet-Visible Quantitative
1
Analysis
This standard is issued under the fixed designation E169; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope Visible Spectrophotometers whose Spectral Bandwidth
does not Exceed 2 nm
1.1 These practices are intended to provide general infor-
E958Practice for Estimation of the Spectral Bandwidth of
mation on the techniques most often used in ultraviolet and
Ultraviolet-Visible Spectrophotometers
visible quantitative analysis. The purpose is to render unnec-
essary the repetition of these descriptions of techniques in
3. Summary of Practice
individual methods for quantitative analysis.
3.1 Quantitative ultraviolet and visible analyses are based
1.2 The values stated in SI units are to be regarded as
upontheabsorptionlaw,knownasBeer’slaw.Theunitsofthis
standard. No other units of measurement are included in this
law are defined in Terminology E131. Beer’s law (Note 1)
standard.
holds at a single wavelength and when applied to a single
1.3 This standard does not purport to address all of the
component sample it may be expressed in the following form
safety concerns, if any, associated with its use. It is the
(see Section 11):
responsibility of the user of this standard to establish appro-
A 5 abc (1)
priate safety, health, and environmental practices and deter-
mine the applicability of regulatory limitations prior to use.
Whenappliedtoamixtureof nnon-interactingcomponents,
1.4 This international standard was developed in accor-
it may be expressed as follows:
dance with internationally recognized principles on standard-
A 5 a bc 1a bc 1….1a bc (2)
1 1 2 2 n n
ization established in the Decision on Principles for the
Development of International Standards, Guides and Recom-
mendations issued by the World Trade Organization Technical
NOTE 1—Detailed discussion of the origin and validity of Beer’s law
maybefoundinthebooksandarticleslistedinthebibliographyattheend
Barriers to Trade (TBT) Committee.
of these practices.
2. Referenced Documents
3.2 This practice describes the application of Beer’s law in
2
typical spectrophotometric analytical applications. It also de-
2.1 ASTM Standards:
scribes operating parameters that must be considered when
E131Terminology Relating to Molecular Spectroscopy
using these techniques.
E168Practices for General Techniques of Infrared Quanti-
tative Analysis
4. Significance and Use
E275PracticeforDescribingandMeasuringPerformanceof
Ultraviolet and Visible Spectrophotometers
4.1 These practices are a source of general information on
E387TestMethodforEstimatingStrayRadiantPowerRatio
the techniques of ultraviolet and visible quantitative analyses.
of Dispersive Spectrophotometers by the Opaque Filter
Theyprovidetheuserwithbackgroundinformationthatshould
Method
help ensure the reliability of spectrophotometric measure-
E925Practice for Monitoring the Calibration of Ultraviolet-
ments.
4.2 These practices are not intended as a substitute for a
thorough understanding of any particular analytical method. It
1
These practices are under the jurisdiction of ASTM Committee E13 on
Molecular Spectroscopy and Separation Science and are the direct responsibility of is the responsibility of the users to familiarize themselves with
Subcommittee E13.01 on Ultra-Violet, Visible, and Luminescence Spectroscopy.
the critical details of a method and the proper operation of the
Current edition approved Nov. 1, 2022. Published November 2022. Originally
available instrumentation.
approved in 1960. Last previous edition approved in 2016 as E169–16. DOI:
10.1520/E0169-16R22.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or 5. Sample Preparation
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
5.1 Accurately weigh the specified amount of the sample
Standards volume information, refer to the standard’s Document Summary page on
the ASTM website. (solid or liquid). Dissolve in the appropriate solvent and dilute
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
E169 − 16 (2022)
tothespecifiedvolumeinvolumetricglasswareoftherequired uncertainty in the absorbance measurement is within accept-
accuracy, ensuring that all appropriate temperature range able li
...

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