Animal feeding stuffs - Determination of decoquinate by HPLC with fluorescence detection

This European Standard specifies a method for the determination of decoquinate. This high-performance liquid chromatographic (HPLC) method with a fluorescence detection is applicable to the quantification of decoquinate content in complete and complementary compound feeds, medicated feeds, semi-liquid feeds, premixtures and feed additives.
The method was fully validated from LOQ to 60 000 mg/kg on different matrices during an international collaborative study [11], especially on complete compound feeds for poultry, at trace contamination level of 3 mg/kg and at European authorized level of 20 mg/kg to 40 mg/kg [12].
The limit of detection is between 0,1 mg/kg and 0,3 mg/kg and the limit of quantification is around 0,5 mg/kg. These limits were validated during the collaborative study [11], from results on the blank feed. Lower limits of detection or quantification could be reached but a single laboratory validation is then requested.

Futtermittel - Bestimmung von Decoquinat mit Hochleistungs-Flüssigchromatographie (HPLC) und Fluoreszenzdetektion

Diese Europäische Norm legt ein Verfahren zur Bestimmung von Decoquinat fest. Dieses hochleistungsflüssigchromatographische (HPLC) Verfahren mit Fluoreszenzdetektion ist für die Quantifizierung des Decoquinatgehaltes in Allein- und Ergänzungsfuttermitteln, Medizinalfutter, semi-Flüssigfutter, Vormischungen und Futterzusatzstoffen geeignet.
Das Verfahren wurde vollständig für verschiedene Matrices für den Bereich von LOQ bis 60 000 mg/kg während eines international durchgeführten Ringversuches [11] validiert, besonders für Alleinfuttermittel für Geflügel, bei einem Spurenverunreinigungsgehalt von 3 mg/kg und bei einem europäisch zugelassenen Gehalt von 20 mg/kg bis 40 mg/kg [12].
Die Nachweisgrenze liegt zwischen 0,1 mg/kg und 0,3 mg/kg und die Bestimmungsgrenze ungefähr bei 0,5 mg/kg. Diese Grenzen wurden während des Ringversuchs [11] anhand der mit dem Kontrollfuttermittel ohne Zusatz erzielten Ergebnisse validiert. Niedrigere Nachweis  und Bestimmungsgrenzen könnten erreicht werden, jedoch ist dann eine Validierung durch ein Einzellaboratorium erforderlich.

Aliments des animaux - Dosage du décoquinate par chromatographie liquide à haute performance (CLHP) avec détection par fluorescence

La présente Norme européenne spécifie une méthode de dosage du décoquinate. La présente méthode de chromatographie liquide à haute performance (CLHP) avec détection par fluorescence est applicable à la détermination de la teneur en décoquinate dans les aliments composés complets et complémentaires pour animaux, les aliments médicamenteux, les aliments semi-liquides, les prémélanges et additifs utilisés en alimentation animale.
La méthode a été validée dans sa totalité, de la limite de quantification à 60 000 mg/kg, selon différentes matrices, lors d’un essai interlaboratoires [11], en particulier pour les aliments complets pour volailles, au niveau de la contamination à l’état de traces de 3 mg/kg et à la teneur autorisée en Europe comprise entre 20 mg/kg et 40 mg/kg [12].
La limite de détection est comprise entre 0,1 mg/kg et 0,3 mg/kg et la limite de quantification est d’environ 0,5 mg/kg. Ces limites ont été validées lors d’un essai interlaboratoires [11], à partir des résultats obtenus sur l’aliment témoin. Des limites de détection ou de quantification inférieures pourraient être atteintes à condition d’être validées en interne (validation intralaboratoire).

Krma - Določevanje dekokvinata s HPLC s fluorescentno detekcijo

Ta evropski standard določa metodo za določevanje dekokvinata. Ta metoda tekočinske kromatografije visoke ločljivosti (HPLC) s fluorescenčnim zaznavanjem se uporablja za kvantifikacijo vsebnosti dekokvinata v polnovrednih in dopolnilnih krmnih mešanicah, medicirani krmi, poltekoči krmi, premiksih in krmnih dodatkih. Metoda je bila z mednarodno medlaboratorijsko študijo v celoti validirana za razpon od meje kvantifikacije do 60.000 mg/kg na različnih matricah, zlasti na polnovrednih krmnih mešanicah za perutnino pri vrednosti onesnaženja v sledovih 3 mg/kg in evropski dovoljeni vrednosti 20 mg/kg do 40 mg/kg. Meja detekcije je med 0,1 mg/kg in 0,3 mg/kg, meja kvantifikacije pa približno 0,5 mg/kg. Ti meji sta bili validirani z medlaboratorijsko študijo na podlagi rezultatov iz slepe krme. Doseči je mogoče nižjo mejo detekcije ali kvantifikacije, vendar se za to zahteva ena sama laboratorijska validacija.

General Information

Status
Published
Publication Date
20-Mar-2012
Current Stage
9060 - Closure of 2 Year Review Enquiry - Review Enquiry
Start Date
04-Mar-2023
Completion Date
04-Mar-2023

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Standards Content (Sample)

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Futtermittel - Bestimmung von Decoquinat mit Hochleistungs-Flüssigchromatographie (HPLC) und FluoreszenzdetektionAliments des animaux - Dosage du décoquinate par CLHP avec détection par fluorescenceAnimal feeding stuffs - Determination of Decoquinate by HPLC with fluorescence detection65.120KrmilaAnimal feeding stuffsICS:Ta slovenski standard je istoveten z:EN 16162:2012SIST EN 16162:2012en,fr,de01-september-2012SIST EN 16162:2012SLOVENSKI
STANDARD



SIST EN 16162:2012



EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 16162
March 2012 ICS 65.120 English Version
Animal feeding stuffs - Determination of decoquinate by HPLC with fluorescence detection
Aliments des animaux - Détermination du décoquinate par Chromatographie Liquide Haute Performance avec détection fluorimétrique
Futtermittel - Bestimmung von Decoquinat mit Hochleistungs-Flüssigchromatographie (HPLC) und Fluoreszenzdetektion This European Standard was approved by CEN on 4 February 2012.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre:
Avenue Marnix 17,
B-1000 Brussels © 2012 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 16162:2012: ESIST EN 16162:2012



EN 16162:2012 (E) 2 Contents Page Foreword .4Introduction .51Scope .62Normative references .63Principle .64Reagents .65Apparatus .86Sampling .87Sample preparation .88Procedure .88.1General .88.2Extraction of feeds (decoquinate content between 10 mg/kg to 500 mg/kg) .88.3Extraction of complementary feeds, premixtures and feed additives (decoquinate content higher than 500 mg/kg) .98.4Extraction of trace feeds (decoquinate content lower than 10 mg/kg) .98.5Quality control spiked feeds .98.5.1Blank Feed to spike at 30 mg/kg .98.5.2Blank feed to spike at 9 mg/kg .
...

SLOVENSKI STANDARD
oSIST prEN 16162:2011
01-februar-2011
.UPD'RORþHYDQMHGHNRNYLQDWDV+3/&VIOXRUHVFHQWQRGHWHNFLMR
Animal feeding stuffs - Determination of Decoquinate by HPLC with fluorescence
detection
Futtermittel - Bestimmung von Decoquinat mit Hochleistungs-Flüssigchromatographie
(HPLC) und Fluoreszenzdetektion
Aliments des animaux - Dosage du décoquinate par CLHP avec détection par
fluorescence
Ta slovenski standard je istoveten z: prEN 16162
ICS:
65.120 Krmila Animal feeding stuffs
oSIST prEN 16162:2011 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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oSIST prEN 16162:2011

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oSIST prEN 16162:2011


EUROPEAN STANDARD
DRAFT
prEN 16162
NORME EUROPÉENNE

EUROPÄISCHE NORM

October 2010
ICS 65.120
English Version
Animal feeding stuffs - Determination of Decoquinate by HPLC
with fluorescence detection
Aliments des animaux - Dosage du décoquinate par CLHP Futtermittel - Bestimmung von Decoquinat mit
avec détection par fluorescence Hochleistungs-Flüssigchromatographie (HPLC) und
Fluoreszenzdetektion
This draft European Standard is submitted to CEN members for enquiry. It has been drawn up by the Technical Committee CEN/TC 327.

If this draft becomes a European Standard, CEN members are bound to comply with the CEN/CENELEC Internal Regulations which
stipulate the conditions for giving this European Standard the status of a national standard without any alteration.

This draft European Standard was established by CEN in three official versions (English, French, German). A version in any other language
made by translation under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the
same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.


Warning : This document is not a European Standard. It is distributed for review and comments. It is subject to change without notice and
shall not be referred to as a European Standard.


EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2010 CEN All rights of exploitation in any form and by any means reserved Ref. No. prEN 16162:2010: E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------
oSIST prEN 16162:2011
prEN 16162:2010 (E)
Contents Page
Foreword .3
Introduction .3
1 Scope .4
2 Normative references .4
3 Principle .4
4 Reagents .4
5 Apparatus .5
6 Sampling .6
7 Sample preparation .6
8 Procedure .6
9 Calculations .8
10 Precision .8
11 Test report .9
Annex A (informative
...

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