EN ISO 30024:2024
(Main)Animal feeding stuffs - Determination of phytase activity (ISO 30024:2024)
Animal feeding stuffs - Determination of phytase activity (ISO 30024:2024)
This document specifies the determination of phytase activity in feeding stuff samples, including feed raw materials from plant origin, compound feeds (complete, complementary, mineral feeds), premixtures and feed additives.
The method is applicable to, and is collaboratively validated for, the determination of phytase activity in complete feed, complementary feed including mineral feed, premixtures and feed additives.
The method does not distinguish between phytase added as a feed additive and endogenous phytase already present in the feed materials. Therefore, the method is also applicable for feed materials from plant origin.
The method does not apply to evaluating or comparing the in vivo efficacy of the phytase product. It is not a predictive method of the in vivo efficacy of phytases present on the market as they can develop different in vivo efficacy per unit of activity.
Futtermittel - Bestimmung der Phytaseaktivität (ISO 30024:2024)
Dieses Dokument legt die Bestimmung der Phytaseaktivität in Futtermittelproben, einschließlich Futtermittel-Ausgangsstoffen pflanzlichen Ursprungs, Mischfuttermitteln (Allein-, Ergänzungs- und Mineralfuttermittel), Vormischungen und Futtermittelzusatzstoffen, fest.
Das Verfahren wurde in einem Ringversuch validiert und ist anwendbar für die Bestimmung der Phytaseaktivität in Alleinfuttermittel, Ergänzungsfuttermittel einschließlich Mineralfuttermittel, Vormischungen und Futtermittelzusatzstoffen.
Das Verfahren unterscheidet nicht zwischen Phytase, die als Futtermittelzusatzstoff zugefügt wurde und endogener Phytase, die bereits in den Futtermittel-Ausgangsstoffen vorhanden war. Daher ist das Verfahren auch für Futtermittel-Ausgangsstoffe pflanzlichen Ursprungs anwendbar.
Das Verfahren ist nicht anwendbar für die Bewertung oder den Vergleich der in vivo-Wirksamkeit von Phytase-Produkten. Es dient auch nicht als Verfahren zur Vorhersage der in vivo-Wirksamkeit von auf dem Markt angebotenen Phytasen, da sich diese in vivo-Wirksamkeit in Abhängigkeit von der Einheit für die Aktivität unterschiedlich entwickeln kann.
Alimentation animale - Détermination de l'activité phytasique (ISO 30024:2024)
Le présent document spécifie la détermination de l’activité phytasique dans les échantillons d’aliments pour animaux, y compris dans les matières premières d’origine végétale, aliments composés (complets, complémentaires et minéraux), prémélanges et additifs destinés à l’alimentation animale.
La méthode est applicable et validée de manière collaborative pour la détermination de l’activité phytasique dans les aliments complets, les aliments complémentaires, y compris les aliments minéraux, les prémélanges et les additifs, destinés à l’alimentation animale.
La méthode ne fait pas la distinction entre la phytase ajoutée en tant qu’additif destiné à l’alimentation animale et la phytase endogène déjà présente dans les matières premières pour l’alimentation animale. Par conséquent, la méthode est également applicable aux matières premières d’origine végétale pour l’alimentation animale.
La méthode ne s’applique pas pour évaluer ou comparer l’efficacité in vivo des phytases. Il ne s’agit pas d’une méthode prédictive de l’efficacité in vivo des phytases disponibles sur le marché, car celles-ci peuvent développer une efficacité in vivo différente par unité d’activité.
Krma - Določanje aktivnosti fitaze (ISO 30024:2024)
General Information
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Standards Content (Sample)
SLOVENSKI STANDARD
01-julij-2024
Krma - Določanje aktivnosti fitaze (ISO 30024:2024)
Animal feeding stuffs - Determination of phytase activity (ISO 30024:2024)
Futtermittel - Bestimmung der Phytaseaktivität (ISO 30024:2024)
Alimentation animale - Détermination de l'activité phytasique (ISO 30024:2024)
Ta slovenski standard je istoveten z: EN ISO 30024:2024
ICS:
65.120 Krmila Animal feeding stuffs
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN ISO 30024
EUROPEAN STANDARD
NORME EUROPÉENNE
May 2024
EUROPÄISCHE NORM
ICS 65.120 Supersedes EN ISO 30024:2009
English Version
Animal feeding stuffs - Determination of phytase activity
(ISO 30024:2024)
Alimentation animale - Détermination de l'activité Futtermittel - Bestimmung der Phytaseaktivität (ISO
phytasique (ISO 30024:2024) 30024:2024)
This European Standard was approved by CEN on 21 April 2024.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2024 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 30024:2024 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
European foreword
This document (EN ISO 30024:2024) has been prepared by Technical Committee ISO/TC 34 "Food
products" in collaboration with Technical Committee CEN/TC 327 “Animal feeding stuffs - Methods of
sampling and analysis” the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by November 2024, and conflicting national standards
shall be withdrawn at the latest by November 2024.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 30024:2009.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
United Kingdom.
Endorsement notice
The text of ISO 30024:2024 has been approved by CEN as EN ISO 30024:2024 without any modification.
International
Standard
ISO 30024
Second edition
Animal feeding stuffs —
2024-04
Determination of phytase activity
Alimentation animale — Détermination de l'activité phytasique
Reference number
ISO 30024:2024(en) © ISO 2024
ISO 30024:2024(en)
© ISO 2024
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
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Published in Switzerland
ii
ISO 30024:2024(en)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Reagents . 3
6 Apparatus . 5
7 Sampling and sample preparation . 5
8 Sample extractions . 6
8.1 For compound feeds (excluding mineral feeds) .6
8.2 For mineral feeds and premixtures .6
8.3 For feed additives .6
9 Procedure . 7
9.1 General .7
9.2 Blank solution .7
9.3 Standards .7
9.3.1 Phosphate standard solution.7
9.3.2 Phytase level control.7
9.4 Standard curve .7
9.5 Phytase level control .8
9.6 For compound feed (excluding mineral feeds) .9
9.7 For mineral feeds and premixtures .10
9.8 For feed additives .10
10 Calculations .11
10.1 Formulation of standard curve .11
10.2 Calculation of phytase activity. 12
10.3 Correction for phytic acid purity and water content . 13
10.4 Interference with blank values .14
11 Precision . 14
11.1 Limit of detection and limit of quantification .14
11.2 Interlaboratory tests .14
11.3 Repeatability .14
11.4 Reproducibility . 15
12 Test report .15
Annex A (informative) Interlaboratory study results for compound feeds (excluding mineral
feeds) . 16
Annex B (informative) Interlaboratory study results for mineral feeds and premixtures . 19
Annex C (informative) Interlaboratory study results for feed additives .20
Bibliography .22
iii
ISO 30024:2024(en)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
ISO technical committees. Each member body interested in a subject for which a technical committee
has been established has the right to be represented on that committee. International organizations,
governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely
with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
The procedu
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