EN ISO 6887-1:2017/A1:2024

Microbiology of the food chain - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 1: General rules for the preparation of the initial suspension and decimal dilutions - Amendment 1: Requirements and guidance on the use of a larger test portion size for qualitative methods (ISO 6887-1:2017/Amd 1:2024)

Mikrobiologie der Lebensmittelkette - Vorbereitung von Untersuchungsproben und Herstellung von Erstverdünnungen und von Dezimalverdünnungen für mikrobiologische Untersuchungen - Teil 1: Allgemeine Regeln für die Herstellung von Erstverdünnungen und Dezimalverdünnungen - Änderung 1: Anforderungen und Leitlinien für die Verwendung größerer Prüfmengen bei qualitativen Verfahren (ISO 6887 1:2017/Amd 1:2024)

Microbiologie de la chaîne alimentaire - Préparation des échantillons, de la suspension mère et des dilutions décimales en vue de l'examen microbiologique - Partie 1: Règles générales pour la préparation de la suspension mère et des dilutions décimales - Amendement 1: Exigences et recommandations sur l’utilisation d’une taille de prise d’essai plus grande pour les méthodes qualitatives (ISO 6887-1:2017/Amd 1:2024)

Mikrobiologija v prehranski verigi - Priprava preskusnih vzorcev, osnovne suspenzije in decimalnih razredčin za mikrobiološko preiskavo - 1. del: Splošna pravila za pripravo osnovne suspenzije in decimalnih razredčin - Dopolnilo A1: Zahteve in navodila o uporabi testnega vzorca večje velikosti za kvalitativne metode (ISO 6887-1:2017/Amd 1:2024)

General Information

• Status: Published
• Publication Date: 30-Jul-2024
• ICS: 07.100.30 - Food microbiology
• Technical Committee: CEN/TC 463 - Microbiology of the food chain
• Drafting Committee: CEN/TC 463 - Microbiology of the food chain
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 31-Jul-2024
• Completion Date: 31-Jul-2024
• Ref Project: SIST EN ISO 6887-1:2017/A1:2024 - Microbiology of the food chain - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 1: General rules for the preparation of the initial suspension and decimal dilutions - Amendment 1: Requirements and guidance on the use of larger test portion size for qualitative method (ISO 6887-1:2017/Amd 1:2024)

Relations

• Amends: EN ISO 6887-1:2017 - Microbiology of the food chain - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 1: General rules for the preparation of the initial suspension and decimal dilutions (ISO 6887-1:2017)
• Effective Date: 23-Jun-2021

Standards Content (Sample)

SLOVENSKI STANDARD
01-oktober-2024
Mikrobiologija v prehranski verigi - Priprava preskusnih vzorcev, osnovne
suspenzije in decimalnih razredčin za mikrobiološko preiskavo - 1. del: Splošna
pravila za pripravo osnovne suspenzije in decimalnih razredčin - Dopolnilo A1:
Zahteve in navodila o uporabi testnega vzorca večje velikosti za kvalitativne
metode (ISO 6887-1:2017/Amd 1:2024)
Microbiology of the food chain - Preparation of test samples, initial suspension and
decimal dilutions for microbiological examination - Part 1: General rules for the
preparation of the initial suspension and decimal dilutions - Amendment 1: Requirements
and guidance on the use of larger test portion size for qualitative method (ISO 6887-
1:2017/Amd 1:2024)
Mikrobiologie der Lebensmittelkette - Vorbereitung von Untersuchungsproben und
Herstellung von Erstverdünnungen und von Dezimalverdünnungen für mikrobiologische
Untersuchungen - Teil 1: Allgemeine Regeln für die Herstellung von Erstverdünnungen
und Dezimalverdünnungen - Änderung 1: Anforderungen und Leitlinien für die
Verwendung größerer Prüfmengen bei qualitativen Verfahren (ISO 6887-1:2017/Amd
1:2024)
Microbiologie de la chaîne alimentaire - Préparation des échantillons, de la suspension
mère et des dilutions décimales en vue de l'examen microbiologique - Partie 1: Règles
générales pour la préparation de la suspension mère et des dilutions décimales -
Amendement 1: Exigences et recommandations sur l’utilisation d’une taille de prise
d’essai plus grande pour les méthodes qualitatives (ISO 6887-1:2017/Amd 1:2024)
Ta slovenski standard je istoveten z: EN ISO 6887-1:2017/A1:2024
ICS:
07.100.30 Mikrobiologija živil Food microbiology
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 6887-1:2017/A1
EUROPEAN STANDARD
NORME EUROPÉENNE
July 2024
EUROPÄISCHE NORM
ICS 07.100.30
English Version
Microbiology of the food chain - Preparation of test
samples, initial suspension and decimal dilutions for
microbiological examination - Part 1: General rules for the
preparation of the initial suspension and decimal dilutions
- Amendment 1: Requirements and guidance on the use of
larger test portion size for qualitative method (ISO 6887-
1:2017/Amd 1:2024)
Microbiologie de la chaîne alimentaire - Préparation Mikrobiologie der Lebensmittelkette - Vorbereitung
des échantillons, de la suspension mère et des dilutions von Untersuchungsproben und Herstellung von
décimales en vue de l'examen microbiologique - Partie Erstverdünnungen und von Dezimalverdünnungen für
1: Règles générales pour la préparation de la mikrobiologische Untersuchungen - Teil 1: Allgemeine
suspension mère et des dilutions décimales - Regeln für die Herstellung von Erstverdünnungen und
Amendement 1: Exigences et recommandations sur Dezimalverdünnungen - Änderung 1: Anforderungen
l'utilisation d'une taille de prise d'essai plus grande und Leitlinien für die Verwendung größerer
pour les méthodes qualitatives (ISO 6887-1:2017/Amd Prüfmengen bei qualitativen Verfahren (ISO 6887
1:2024) 1:2017/Amd 1:2024)
This amendment A1 modifies the European Standard EN ISO 6887-1:2017; it was approved by CEN on 25 July 2024.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for inclusion of
this amendment into the relevant national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This amendment exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the
same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2024 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 6887-1:2017/A1:2024 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 6887-1:2017/A1:2024) has been prepared by Technical Committee ISO/TC 34
"Food products" in collaboration with Technical Committee CEN/TC 463 “Microbiology of the food
chain” the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by January 2025, and conflicting national standards shall
be withdrawn at the latest by January 2025.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
United Kingdom.
Endorsement notice
The text of ISO 6887-1:2017/Amd 1:2024 has been approved by CEN as EN ISO 6887-1:2017/A1:2024
without any modification.
International
Standard
ISO 6887-1
Second edition
Microbiology of the food chain —
2017-03
Preparation of test samples, initial
suspension and decimal dilutions
AMENDMENT 1
for microbiological examination —
2024-07
Part 1:
General rules for the preparation of
the initial suspension and decimal
dilutions
AMENDMENT 1: Requirements and
guidance on the use of a larger test
portion size for qualitative methods
Microbiologie de la chaîne alimentaire — Préparation des
échantillons, de la suspension mère et des dilutions décimales en
vue de l'examen microbiologique —
Partie 1: Règles générales pour la préparation de la suspension
mère et des dilutions décimales
AMENDEMENT 1: Exigences et recommandations sur l’utilisation
d’une taille de prise d’essai plus grande pour les méthodes
qualitatives
Reference number
ISO 6887-1:2017/Amd.1:2024(en) © ISO 2024

ISO 6887-1:2017/Amd.1:2024(en)
© ISO 2024
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: [phone]
Email: [e-mail]
Website: www.iso.org
Published in Switzerland
ii
ISO 6887-1:2017/Amd.1:2024(en)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
ISO technical committees. Each member body interested in a subject for which a technical committee
has been established has the right to be represented on that committee. International organizations,
governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely
with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are described
in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the different types
of ISO document should be noted. This document was drafted in accordance with the editorial rules of the
ISO/IEC Directives, Part 2 (see www.iso.org/directives).
ISO draws attention to the possibility that the implementation of this document may involve the use of (a)
patent(s). ISO takes no position concerning the evidence, validity or applicability of any claimed patent
rights in respect thereof. As of the date of publication of this document, ISO had not received notice of (a)
patent(s) which may be required to implement this document. However, implementers are cautioned that
this may not represent the latest information, which may be obtained from the patent database available at
www.iso.org/patents. ISO shall not be held responsible for identifying any or all such patent rights.
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and expressions
related to conformity assessment, as well as information about ISO’s adherence to the World Trade
Organization (WTO) principles in the Technical Barriers to Trade (TBT), see www.iso.org/iso/foreword.html.
This document was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 9,
Microbiology, in collaboration with the European Committee for Standardization (CEN) Technical Committee
CEN/TC 463, Microbiology of the food chain, in accordance with the Agreement on technical cooperation
between ISO and CEN (Vienna Agreement).
A list of parts in the ISO 6887 series can be found on the ISO website.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.

iii
ISO 6887-1:2017/Amd.1:2024(en)
Microbiology of the food chain — Preparation of test samples,
initial suspension and decimal dilutions for microbiological
examination —
Part 1:
General rules for the preparation of the initial suspension
and decimal dilutions
AMENDMENT 1: Requirements and guidance on the use of a larger
test portion size for qualitative methods

Clause 2
Add the following normative references:
ISO 16140-4:2020, Microbiology of the food chain — Method validation — Part 4: Protocol for method validation
in a single laboratory
ISO 16140-4:2020/Amd 1:2024, Microbiology of the food chain — Method validation — Part 4: Protocol for method
validation in a single laboratory — Amendment 1: Validation of a larger test portion size for qualitative methods

3.2
Replace the text with the following:
3.2
composite sample
mixed sample of a number of the same items (3.13) of food, animal feed, animals or environment, prepared
in or out of the laboratory from which a test portion is taken for examination
Note 1 to entry: See Figure A.1 for an illustration of a composite sample.

3.3
Replace the text with the following:
3.3
pooled sample
mixed sample of a number of the same items (3.13) of food, animal feed, animals or environment, prepared
in or out of the laboratory where the complete mixture is the test portion and is taken as a whole for
examination
Note 1 to entry: See Figure A.1 for an illustration of a pooled sample

Add the following terminological entries:

ISO 6887-1:2017/Amd.1:2024(en)
3.11
category
group of sample types (3.12) of the same origin
Note 1 to entry: Food categories are listed in ISO 16140-2:2016, Table A.1.
EXAMPLE Heat-processed milk and dairy products.
[SOURCE: ISO 16140-1:2016, 2.11, modified — Note 1 to entry added.]
3.12
type
for a given category (3.11), a group of items (3.13) processed in a similar way, with similar intrinsic
characteristics and a similar microbial ecology
Note 1 to entry: Food types are listed in ISO 16140-2:2016, Table A.1.
EXAMPLE Food category: heat-processed milk and dairy products; food type: pasteurized dairy product.
[SOURCE: ISO 16140-1:2016, 2.78, modified — Note 1 to entry added.]
3.13
item
single specified food, feed, environmental or primary production matrix
Note 1 to entry: Examples for food items are listed in ISO 16140-2:2016, Table A.1.
EXAMPLE Food category: heat-processed milk and dairy products; food type: pasteurized dairy product; food
item: milk-based desserts.
[SOURCE: ISO 16140-1:2016, 2.34, modified — Note 1 to entry added.]
3.14
larger test portion
measured (volume or mass) representative sample taken from the laboratory sample (3.1) or test sample
(3.4) for use in the preparation of the initial suspension that is larger than the test portion that has been
described in the original method and/or validation document

9.3
Replace the text with the following:
9.3  Composite sample and larger test portion
9.3.1  Composite sample
A composite sample is where a number of the same items
...