EN ISO 6887-1:2017
(Main)Microbiology of the food chain - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 1: General rules for the preparation of the initial suspension and decimal dilutions (ISO 6887-1:2017)
Microbiology of the food chain - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 1: General rules for the preparation of the initial suspension and decimal dilutions (ISO 6887-1:2017)
ISO 6887-1:2017 defines general rules for the aerobic preparation of the initial suspension and of dilutions for microbiological examinations of products intended for human or animal consumption.
ISO 6887-1:2017 is applicable to the general case and other parts apply to specific groups of products as mentioned in the foreword. Some aspects might also be applicable to molecular methods where matrices can be associated with inhibition of the PCR steps and consequently affect the test result.
ISO 6887-1:2017 excludes preparation of samples for both enumeration and detection test methods where preparation instructions are detailed in specific International Standards.
Mikrobiologie der Lebensmittelkette - Vorbereitung von Untersuchungsproben und Herstellung von Erstverdünnungen und von Dezimalverdünnungen für mikrobiologische Untersuchungen - Teil 1: Allgemeine Regeln für die Herstellung von Erstverdünnungen und Dezimalverdünnungen (ISO 6887-1:2017)
Dieses Dokument legt allgemeinen Regeln für die aerobe Herstellung von Erstverdünnungen und Verdünnungen für mikrobiologische Untersuchungen von Produkten fest, die für den Verzehr durch Menschen oder Tiere vorgesehen sind.
Dieses Dokument gilt für den allgemeinen Fall und andere Teile gelten für spezifische Produkt¬gruppen, wie im Vorwort dargelegt. Einige Aspekte könnten auch auf molekulare Verfahren anwendbar sein, bei denen Matrices mit der Hemmung von PCR Schritten in Zusammenhang stehen und folglich das Untersuchungsergebnis beeinflussen können.
Dieses Dokument schließt die Vorbereitung von Proben für Untersuchungsverfahren zur Zählung und zum Nachweis aus, bei denen die Anweisungen für die Vorbereitung in spezifischen Internationalen Normen ausführlich dargelegt sind.
Microbiologie de la chaîne alimentaire - Préparation des échantillons, de la suspension mère et des dilutions décimales en vue de l'examen microbiologique - Partie 1: Règles générales pour la préparation de la suspension mère et des dilutions décimales (ISO 6887-1:2017)
ISO 6887-1:2017 définit des règles générales pour la préparation de la suspension mère et des dilutions réalisées en aérobiose, en vue des examens microbiologiques des produits destinés à la consommation humaine ou à l'alimentation animale.
ISO 6887-1:2017 est applicable aux cas généraux et les autres parties s'appliquent aux groupes de produits spécifiques indiqués dans l'Avant-propos. Certains aspects peuvent aussi être applicables aux méthodes moléculaires dans lesquelles les matrices peuvent être impliquées dans l'inhibition des étapes de PCR et, de ce fait, affectent le résultat d'essai.
ISO 6887-1:2017 exclut la préparation d'échantillons en vue des méthodes de dénombrement et de recherche dans lesquelles les instructions de préparation sont détaillées dans des Normes internationales spécifiques.
Mikrobiologija v prehranski verigi - Priprava preskusnih vzorcev, osnovne suspenzije in decimalnih razredčin za mikrobiološko preiskavo - 1. del: Splošna pravila za pripravo osnovne suspenzije in decimalnih razredčin (ISO 6887-1:2017)
Ta dokument določa splošna pravila za aerobno pripravo osnovne suspenzije in razredčin za mikrobiološke preiskave izdelkov, namenjenih za prehrano ljudi ali živali. Ta dokument se uporablja za splošne primere, drugi deli pa veljajo za posebne skupine izdelkov, kot je omenjeno v predgovoru. Nekateri vidiki se lahko uporabljajo tudi za molekularne metode, kjer je mogoče matrice povezati z zaviranjem verižne reakcije s polimerazo, kar vpliva na rezultate preskusa. Ta dokument ne zajema priprave vzorcev za preskusni metodi za ugotavljanje števila in odkrivanje, kadar so navodila glede priprave podrobneje opisana v določenih mednarodnih standardih.
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Standards Content (Sample)
SLOVENSKI STANDARD
01-junij-2017
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SIST EN ISO 6887-1:1999
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Microbiology of the food chain - Preparation of test samples, initial suspension and
decimal dilutions for microbiological examination - Part 1: General rules for the
preparation of the initial suspension and decimal dilutions (ISO 6887-1:2017)
Mikrobiologie der Lebensmittelkette - Vorbereitung von Untersuchungsproben und
Herstellung von Erstverdünnungen und von Dezimalverdünnungen für mikrobiologische
Untersuchungen - Teil 1: Allgemeine Regeln für die Herstellung von Erstverdünnungen
und Dezimalverdünnungen (ISO 6887-1:2017)
Microbiologie des aliments - Préparation des échantillons, de la suspension mère et des
dilutions décimales en vue de l'examen microbiologique - Partie 1: Règles générales
pour la préparation de la suspension mère et des dilutions décimales (ISO 6887-1:2017)
Ta slovenski standard je istoveten z: EN ISO 6887-1:2017
ICS:
07.100.30 Mikrobiologija živil Food microbiology
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN ISO 6887-1
EUROPEAN STANDARD
NORME EUROPÉENNE
April 2017
EUROPÄISCHE NORM
ICS 07.100.30 Supersedes EN ISO 6887-1:1999
English Version
Microbiology of the food chain - Preparation of test
samples, initial suspension and decimal dilutions for
microbiological examination - Part 1: General rules for the
preparation of the initial suspension and decimal dilutions
(ISO 6887-1:2017)
Microbiologie de la chaîne alimentaire - Préparation Mikrobiologie der Lebensmittelkette - Vorbereitung
des échantillons, de la suspension mère et des dilutions von Untersuchungsproben und Herstellung von
décimales en vue de l'examen microbiologique - Partie Erstverdünnungen und von Dezimalverdünnungen für
1: Règles générales pour la préparation de la mikrobiologische Untersuchungen - Teil 1: Allgemeine
suspension mère et des dilutions décimales (ISO 6887- Regeln für die Herstellung von Erstverdünnungen und
1:2017) Dezimalverdünnungen (ISO 6887-1:2017)
This European Standard was approved by CEN on 14 January 2017.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2017 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 6887-1:2017 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
European foreword
This document (EN ISO 6887-1:2017) has been prepared by Technical Committee ISO/TC 34 "Food
products" in collaboration with Technical Committee CEN/TC 275 “Food analysis - Horizontal methods”
the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by October 2017, and conflicting national standards shall
be withdrawn at the latest by October 2017.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent
rights.
This document supersedes EN ISO 6887-1:1999.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and the United Kingdom.
Endorsement notice
The text of ISO 6887-1:2017 has been approved by CEN as EN ISO 6887-1:2017 without any
modification.
INTERNATIONAL ISO
STANDARD 6887-1
Second edition
2017-03
Microbiology of the food chain —
Preparation of test samples, initial
suspension and decimal dilutions for
microbiological examination —
Part 1:
General rules for the preparation of
the initial suspension and decimal
dilutions
Microbiologie de la chaîne alimentaire — Préparation des
échantillons, de la suspension mère et des dilutions décimales en vue
de l’examen microbiologique —
Partie 1: Règles générales pour la préparation de la suspension mère
et des dilutions décimales
Reference number
ISO 6887-1:2017(E)
©
ISO 2017
ISO 6887-1:2017(E)
© ISO 2017, Published in Switzerland
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
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ii © ISO 2017 – All rights reserved
ISO 6887-1:2017(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 3
5 Diluents . 3
5.1 Basic materials . 3
5.2 Diluents for general use . 3
5.2.1 Peptone salt solution . 3
5.2.2 Buffered peptone water . 4
5.2.3 Double-strength buffered peptone water . 4
5.3 Diluents for special purposes . 4
5.4 Distribution and sterilization of the diluent . 4
5.5 Performance testing for diluents . 5
6 Apparatus . 5
7 Sampling . 6
8 Preparation of samples. 6
8.1 General . 6
8.2 Frozen products . 7
8.2.1 General. 7
8.2.2 Small samples defrosted before testing . 7
8.2.3 Large pieces or blocks sampled while frozen . 7
8.3 Hard and dry products . 8
8.4 Dehydrated and other low-moisture products. 8
8.5 Liquid and non-viscous products. 8
8.6 Acidic products . 8
8.7 High-fat (over 20 %) foods . 9
8.8 Multi-component products. 9
8.9 Packaged products . 9
8.10 Surface samples (swabs and other devices) .10
9 Specific procedures.10
9.1 Test portion and initial suspension (primary dilution) .10
9.2 Duration of the procedure .11
9.3 Pooling and compositing procedures for qualitative tests.11
10 Further dilutions .11
10.1 Decimal dilution series .11
10.2 Other dilution series .12
Annex A (informative) Illustrations of pooling and compositing procedures.13
Annex B (informative
...
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