EN ISO 17226-3:2011

Leather - Chemical determination of formaldehyde content - Part 3: Determination of formaldehyde emissions from leather (ISO 17226-3:2011)

ISO 17226-3:2011 specifies a method for determining the emission of formaldehyde from leathers. This method is based on high performance liquid chromatography (HPLC). It is selective and also allows the emission of other low molecular aldehydes and ketones to be observed.
ISO 17226-3:2011 deals with the release of formaldehyde to the gas phase. Therefore, the obtained results are not comparable with the results of methods described in ISO 17726-1 and ISO 17226-2 which are based on extraction with liquid water.

Leder - Chemische Bestimmung des Formaldehydgehalts - Teil 3: Bestimmung der Formaldehydemissionen aus Leder (ISO 17226-3:2011)

Dieser Teil von ISO 17226 legt ein Verfahren zur Bestimmung der Formaldehydemission aus Leder fest. Dieses Verfahren beruht auf der Hochleistungs-Flüssigkeitschromatographie (HPLC). Es ist ein selektives Verfahren und ermöglicht auch das Beobachten der Emission anderer niedermolekularer Aldehyde und Ketone. Dieser Teil von ISO 17226 behandelt die Freisetzung von Formaldehyd in die Gasphase. Folglich sind die erhaltenen Ergebnisse mit den Ergebnissen der in ISO 17226-1 und ISO 17226-2 beschriebenen Verfahren nicht vergleichbar, die auf der Extraktion mit Wasser beruhen.

Cuir - Dosage chimique du formaldéhyde - Partie 3: Dosage du formaldéhyde émis par le cuir (ISO 17226-3:2011)

L'ISO 17226-3:2011 spécifie une méthode pour doser le formaldéhyde émis par les cuirs. Cette méthode repose sur la chromatographie en phase liquide à haute performance (CLHP). Elle est sélective et permet d'observer également les émissions d'autres aldéhydes et cétones de faible poids moléculaire.
L'ISO 17226-3:2011 concerne le formaldéhyde dégagé en phase gazeuse. Les résultats obtenus ne sont donc pas comparables avec ceux des méthodes décrites dans l'ISO 17226-1 et l'ISO 17226‑2, qui sont fondées sur une extraction à l'eau liquide.

Usnje - Kemijsko določevanje formaldehida - 3. del: Določevanje izločenega formaldehida iz usnja (ISO 17226-3:2011)

Ta del ISO 17226 določa metodo za določevanje izločenega formaldehida iz usnja. Ta metoda temelji na tekočinski kromatografiji visoke ločljivosti (HPLC). Je selektivna in omogoča tudi opazovanje izločanja drugih nizkomolekularnih aldehidov in ketonov.

General Information

• Status: Published
• Publication Date: 14-May-2011
• Withdrawal Date: 29-Nov-2011
• ICS: 59.140.30 - Leather and furs
• Technical Committee: CEN/TC 289 - Leather
• Drafting Committee: CEN/TC 289/WG 1 - Chemical test methods
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 15-May-2011
• Due Date: 09-Jun-2012
• Completion Date: 15-May-2011
• Ref Project: SIST EN ISO 17226-3:2011 - Leather - Chemical determination of formaldehyde content - Part 3: Determination of formaldehyde emissions from leather (ISO 17226-3:2011)

Overview - EN ISO 17226-3:2011 (Leather formaldehyde emissions)

EN ISO 17226-3:2011 (ISO 17226-3:2011) specifies a laboratory method for the determination of formaldehyde emissions from leather. The method measures formaldehyde released to the gas phase and captured in water, then quantified by high performance liquid chromatography (HPLC) after derivatization with 2,4‑dinitrophenylhydrazine (DNPH). The standard is selective for formaldehyde and also allows observation of other low‑molecular aldehydes and ketones. Results from this emission method are not comparable with extraction‑based methods in ISO 17226‑1/‑2 or ISO 17726‑1.

Key topics and technical requirements

• Principle: Specimen is suspended above demineralized water in a sealed 1 L polyethylene bottle, incubated at (60 ± 2) °C for (180 ± 15) min. Volatile formaldehyde is absorbed into the water, reacted with DNPH, and analyzed by reversed‑phase HPLC (UV detection, ~360 nm).
• Sampling and specimen size: Six specimens, typically (100 × 40) mm, sampled per ISO 2418 and prepared per ISO 2419. Five for emission tests, one for volatile matter (ISO 4684).
• Reagents and preparation: DNPH solution, acetonitrile, and a formaldehyde stock solution (prepared and standardized by iodine/thiosulfate titration) are specified. Water must meet ISO 3696 grade 3.
• Apparatus: 1 L polyethylene bottles with internal hook, oven (60 °C), HPLC system with UV detector, membrane filters (0.45 µm).
• Procedure essentials: 50 ml demineralized water per bottle; post‑incubation water aliquot mixed with acetonitrile and DNPH, allowed to react (60–180 min), filtered and injected to HPLC. Calibration and recovery checks are required.

Applications and users

EN ISO 17226-3:2011 is used for:

• Quality control and batch testing in tanneries and leather goods manufacturing.
• Product safety assessment for consumer goods (footwear, upholstery, garments) where formaldehyde emissions are regulated.
• Independent testing laboratories and certification bodies validating compliance with chemical safety limits.
• Regulatory and forensic testing when measuring airborne formaldehyde release rather than extractable content.

Users include leather chemists, analytical laboratories, product compliance teams, and R&D departments focused on low‑emission leather finishing.

Related standards

• ISO 17226‑1 (HPLC extraction method) and ISO 17226‑2 (colorimetric extraction) - extraction‑based formaldehyde tests.
• ISO 17726‑1 - other extraction methods (not comparable with emission results).
• ISO 2418, ISO 2419 (sampling and sample preparation), ISO 4684 (volatile matter), ISO 3696 (water quality).

Keywords: EN ISO 17226-3:2011, ISO 17226-3, formaldehyde emissions, leather testing, HPLC, DNPH, leather safety testing.

Standards Content (Sample)

SLOVENSKI STANDARD
01-november-2011
8VQMH.HPLMVNRGRORþHYDQMHIRUPDOGHKLGDGHO'RORþHYDQMHL]ORþHQHJD
IRUPDOGHKLGDL]XVQMD,62
Leather - Chemical determination of formaldehyde content - Part 3: Determination of
formaldehyde emissions from leather (ISO 17226-3:2011)
Leder - Chemische Bestimmung des Formaldehydgehalts - Teil 3: Bestimmung der
Formaldehydemissionen aus Leder (ISO 17226-3:2011)
Cuir - Dosage chimique du formaldéhyde - Partie 3: Dosage des émissions de
formaldéhyde du cuir (ISO 17226-3:2011)
Ta slovenski standard je istoveten z: EN ISO 17226-3:2011
ICS:
59.140.30 Usnje in krzno Leather and furs
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 17226-3
NORME EUROPÉENNE
EUROPÄISCHE NORM
May 2011
ICS 59.140.30
English Version
Leather - Chemical determination of formaldehyde content - Part
3: Determination of formaldehyde emissions from leather (ISO
17226-3:2011)
Cuir - Dosage chimique du formaldéhyde - Partie 3: Leder - Chemische Bestimmung des Formaldehydgehalts -
Dosage du formaldéhyde émis par le cuir (ISO 17226- Teil 3: Bestimmung der Formaldehydemissionen aus Leder
3:2011) (ISO 17226-3:2011)
This European Standard was approved by CEN on 11 May 2011.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.

EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2011 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 17226-3:2011: E
worldwide for CEN national Members.

Contents Page
Foreword .3

Foreword
This document (EN ISO 17226-3:2011) has been prepared by Technical Committee CEN/TC 289 “Leather”,
the secretariat of which is held by UNI, in collaboration with the International Union of Leather Technologists
and Chemists Societies.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by November 2011, and conflicting national standards shall be withdrawn
at the latest by November 2011.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,
Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain,
Sweden, Switzerland and the United Kingdom.

INTERNATIONAL ISO
STANDARD 17226-3
IULTCS/IUC
19-3
First edition
2011-05-15
Leather — Chemical determination of
formaldehyde content —
Part 3:
Determination of formaldehyde emissions
from leather
Cuir — Dosage chimique du formaldéhyde —
Partie 3: Dosage du formaldéhyde émis par le cuir

Reference number
ISO 17226-3:2011(E)
IULTCS/IUC 19-3:2011(E)
©
ISO 2011
ISO 17226-3:2011(E)
IULTCS/IUC 19-3:2011(E)
©  ISO 2011
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means,
electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or
ISO's member body in the country of the requester.
ISO copyright office
Case postale 56 • CH-1211 Geneva 20
Tel. + 41 22 749 01 11
Fax + 41 22 749 09 47
E-mail [e-mail]
Web www.iso.org
Published in Switzerland
ii © ISO 2011 – All rights reserved

ISO 17226-3:2011(E)
IULTCS/IUC 19-3:2011(E)
Contents Page
Foreword .iv
1 Scope.1
2 Normative references.1
3 Principle .1
4 Reagents .1
4.1 Reagents for the formaldehyde stock solution.1
4.2 Reagents for the HPLC method .2
5 Apparatus.2
6 Methods.2
6.1 Procedure for the determination of formaldehyde in the stock solution .2
6.1.1 Preparation of the formaldehyde stock solution .2
6.1.2 Determination .3
6.2 Procedure for the determination of formaldehyde emission by the HPLC method .4
6.2.1 Shipment and storage leather for this method .4
6.2.2 Sampling .4
6.2.3 Determination of volatile matter .4
6.2.4 Determination of formaldehyde emission.4
6.2.5 Reaction with DNPH.4
6.2.6 HPLC conditions (recommendations).4
6.2.7 Calibration of HPLC.5
6.2.8 Calculation of the formaldehyde content in leather samples.5
7 Expression of results.5
8 Test report.6

ISO 17226-3:2011(E)
IULTCS/IUC 19-3:2011(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 17226-3 was prepared by the European Committee for Standardization (CEN) Technical Committee
CEN/TC 289, Leather, in collaboration with Chemical Test Commission of the International Union of Leather
Technologists and Chemists Societies (IUC Commission, IULTCS), in accordance with the Agreement on
technical cooperation between ISO and CEN (Vienna Agreement).
IULTCS, originally formed in 1897, is a world-wide organization of professional leather societies to further the
advancement of leather science and technology. IULTCS has three Commissions, which are responsible for
establishing international methods for the sampling and testing of leather. ISO recognizes IULTCS as an
international standardizing body for the preparation of test methods for leather.
ISO 17226 consists of the following parts, under the general title Leather — Chemical determination of
formaldehyde content:
⎯ Part 1: Method using high performance liquid chromatography
⎯ Part 2: Method using colorimetric analysis
⎯ Part 3: Determination of formaldehyde emissions from leather
iv © ISO 2011 – All rights reserved
...