Plant biostimulants - Determination of the anaerobic plate count

This document provides a horizontal method for enumeration of microorganisms that are able to grow and form colonies in a solid medium after anaerobic incubation at 30 °C.
The method is applicable to microbial plant biostimulants for verifying that the concentration of anaerobes does not exceed the respective limits outlined in the EU Regulation on Fertilising Products [1].
This method does not apply to the microbiological monitoring of the environment in which microbial plant biostimulants are manufactured.
No information about potential human pathogens can be inferred from anaerobic plate counts.

Pflanzen-Biostimulanzien - Bestimmung der anaeroben Keimzahl

Dieses Dokument stellt ein horizontales Verfahren zur Zählung von Mikroorganismen, die nach anaerober Bebrütung bei 30 °C in einem festen Medium wachsen und Kolonien bilden können zur Verfügung.
Das Verfahren ist auf mikrobielle Pflanzen-Biostimulanzien anwendbar, um zu überprüfen, ob die Konzentration der Anaerobier die entsprechenden Grenzwerte der EU Düngeprodukteverordnung [1] nicht überschreitet.
Dieses Verfahren ist nicht anzuwenden für die mikrobiologische Überwachung der Umgebung, in der die mikrobiellen Pflanzen-Biostimulanzien hergestellt werden.
Es können keine Informationen über potentielle humanpathogene Keime aus der Auszählung der anaeroben koloniebildenden Einheiten abgeleitet werden.

Biostimulants des végétaux - Détermination du dénombrement sur plaque des germes anaérobies

Le présent document fournit une méthode horizontale pour le dénombrement des microorganismes qui sont capables de croître et de former des colonies dans un milieu solide après une incubation anaérobie à 30 °C.
La méthode est applicable aux biostimulants microbiens des végétaux pour vérifier que la concentration en anaérobies ne dépasse pas les limites correspondantes indiquées dans le Règlement UE sur les fertilisants [1].
La présente méthode ne s’applique pas à la surveillance microbiologique de l’environnement dans lequel les biostimulants microbiens des végétaux sont fabriqués.
Aucune information sur les pathogènes humains potentiels ne peut être déduite des dénombrements sur plaque des germes anaérobies.

Rastlinski biostimulanti - Določanje števila na anaerobnih mikrotitrskih ploščah

Dokument določa vodoravno metodo za ugotavljanje števila mikroorganizmov, ki so sposobni rasti in tvoriti kolonije v trdnem gojišču po anaerobni inkubaciji pri 30 °C.
Metoda se uporablja za mikrobne rastlinske biostimulante za preverjanje, da koncentracija anaerobov ne presega ustreznih mejnih vrednosti, navedenih v Uredbi EU o sredstvih za gnojenje [1].
Metoda se ne uporablja pri mikrobiološkem nadzoru okolja, v katerem se proizvajajo mikrobni rastlinski biostimulanti.
Na podlagi števila anaerobnih mikrotitrskih plošč ni mogoče sklepati na nobene informacije o potencialnih človeških patogenih.

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Standards Content (Sample)

SIST-TS CEN/TS 17719:2023
Rastlinski biostimulanti - Določanje števila na anaerobnih mikrotitrskih ploščah
Plant biostimulants - Determination of the anaerobic plate count
Pflanzen-Biostimulanzien - Bestimmung der anaeroben Keimzahl
Biostimulants des végétaux - Détermination du dénombrement sur plaque des germes
Ta slovenski standard je istoveten z: CEN/TS 17719:2022
65.080 Gnojila Fertilizers
SIST-TS CEN/TS 17719:2023 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST-TS CEN/TS 17719:2023

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SIST-TS CEN/TS 17719:2023

CEN/TS 17719


March 2022
ICS 65.080
English Version

Plant biostimulants - Determination of the anaerobic plate
 Biostimulanzien für die pflanzliche Anwendung -
Bestimmung der anaeroben Keimzahl
This Technical Specification (CEN/TS) was approved by CEN on 3 January 2022 for provisional application.

The period of validity of this CEN/TS is limited initially to three years. After two years the members of CEN will be requested to
submit their comments, particularly on the question whether the CEN/TS can be converted into a European Standard.

CEN members are required to announce the existence of this CEN/TS in the same way as for an EN and to make the CEN/TS
available promptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in
parallel to the CEN/TS) until the final decision about the possible conversion of the CEN/TS into an EN is reached.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.



CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2022 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN/TS 17719:2022 E
worldwide for CEN national Members.

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SIST-TS CEN/TS 17719:2023
CEN/TS 17719:2022 (E)
Contents Page
European foreword . 4
Introduction . 5
1 Scope . 6
2 Normative references . 6
3 Terms and definitions . 6
4 Principle . 7
4.1 General. 7
4.2 Brief description . 7
5 Culture media and reagents . 7
6 Equipment and consumables . 7
7 Preparation of test sample . 8
7.1 General. 8
7.2 Liquid – water-based formulations . 8
7.3 Liquid – oil-based (emulsifiable concentrate - EC) formulations . 8
7.4 Solid – wettable powder (WP) formulations . 8
7.5 Solid – water dispersible granules (WDG) formulations .

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