This document gives general guidelines for the detection and identification of the specified microorganism Escherichia coli in technical and formulated biostimulant products, both in liquid and solid state, and also the horizontal method for the enumeration of ß-glucuronidase-positive Escherichia coli in plant biostimulants products (both in liquid and solid state).
The qualitative method described in this document is based on the detection of Escherichia coli in a non-selective liquid medium (enrichment broth), followed by isolation on a selective agar medium. Other methods can be appropriate, depending on the level of detection required.
NOTE   For the detection of Escherichia coli, subcultures can be performed on non-selective culture media followed by suitable identification steps (e.g. using identification kits).
The quantitative method described in this document uses a colony-count technique at 44 °C on a solid medium containing a chromogenic ingredient for detection of the enzyme ß-glucuronidase.
WARNING — Strains of Escherichia coli which do not grow at 44 °C and, in particular, those that are ß glucuronidase negative, such as Escherichia coli O157, will not be detected.

  • Technical specification
    20 pages
    English language
    sale 10% off
    e-Library read for
    1 day
  • Draft
    21 pages
    English language
    sale 10% off
    e-Library read for
    1 day

This document provides the methodology for the enumeration and determination of Rhizobium sp., Mesorhizobium sp., Ensifer sp., or Bradyrhizobium sp. in plant biostimulant products in accordance with the Regulation (EU) 2019/1009 of the European Parliament and of the Council [1].

  • Technical specification
    16 pages
    English language
    sale 10% off
    e-Library read for
    1 day
  • Draft
    15 pages
    English language
    sale 10% off
    e-Library read for
    1 day

This document specifies a horizontal method for the enumeration of yeasts and moulds present in plant biostimulants intended for use in agriculture, by means of the colony count technique after aerobic incubation at 25 °C ± 2,5 °C.
This document allows the enumeration of yeasts and moulds, in technical and formulated plant biostimulant, both in liquid and solid state. The method is applicable to microbial plant biostimulant except those composed of fungi or yeast to verify that the concentration of yeast and moulds does not exceed the respective limits described in the EU Fertilisers Regulation [1].
If necessary, yeast and mould enumerated can be identified using suitable identification tests.

  • Technical specification
    17 pages
    English language
    sale 10% off
    e-Library read for
    1 day
  • Draft
    18 pages
    English language
    sale 10% off
    e-Library read for
    1 day

This document specifies general rules to determine the concentration of microorganisms present in plant biostimulant products.
The method is applicable to microbial plant biostimulants for verifying that the concentration of microorganisms does not exceed the respective limits outlined in the EU Regulation on Fertilising Products [1].
This horizontal method might not be appropriate in every detail for certain products. In this case, it is necessary to refer to the methodology of specific determination and quantification of the microorganisms.

  • Technical specification
    19 pages
    English language
    sale 10% off
    e-Library read for
    1 day
  • Draft
    21 pages
    English language
    sale 10% off
    e-Library read for
    1 day

This document provides a method for verifying that the pathogen Staphylococcus aureus is present in microbial plant biostimulants according to the limits outlined in the EU Regulation on Fertilising Products [2].

  • Technical specification
    13 pages
    English language
    sale 10% off
    e-Library read for
    1 day
  • Draft
    14 pages
    English language
    sale 10% off
    e-Library read for
    1 day

This document specifies a method for laboratory measurement of the pH value in liquid microbial plant biostimulants, using pH electrodes with a glass membrane.
From the scope of this document plant biostimulants other than microbial plant biostimulants are excluded because there is no essential requirement in the Regulation (EU) 2019/1009 [1] for measuring the pH of non-microbial plant biostimulants.

  • Technical specification
    15 pages
    English language
    sale 10% off
    e-Library read for
    1 day
  • Draft
    15 pages
    English language
    sale 10% off
    e-Library read for
    1 day

This document was developed to provide a horizontal method for enumeration and genera/species determination [1], [2], [3] of mycorrhizal fungi in plant biostimulants products in accordance with the EU Fertilising Products Regulation.

  • Technical specification
    40 pages
    English language
    sale 10% off
    e-Library read for
    1 day
  • Draft
    37 pages
    English language
    sale 10% off
    e-Library read for
    1 day

This document provides a method for verifying that the pathogen Shigella spp. is not present in microbial plant biostimulants in a concentration that exceeds the respective limits outlined in the EU Regulation on Fertilising Products.
The detection method for Shigella pathogens is not sensitive and quantification is rarely performed. Detection is usually performed using an enrichment medium followed by subculturing onto a variety of selective media.

  • Technical specification
    27 pages
    English language
    sale 10% off
    e-Library read for
    1 day
  • Draft
    28 pages
    English language
    sale 10% off
    e-Library read for
    1 day

This document provides a horizontal method for enumeration of microorganisms that are able to grow and form colonies in a solid medium after anaerobic incubation at 30 °C.
The method is applicable to microbial plant biostimulants for verifying that the concentration of anaerobes does not exceed the respective limits outlined in the EU Regulation on Fertilising Products [1].
This method does not apply to the microbiological monitoring of the environment in which microbial plant biostimulants are manufactured.
No information about potential human pathogens can be inferred from anaerobic plate counts.

  • Technical specification
    14 pages
    English language
    sale 10% off
    e-Library read for
    1 day
  • Draft
    13 pages
    English language
    sale 10% off
    e-Library read for
    1 day

This document describes a method for the detection of Salmonella spp. in biostimulants of the following Product Function Categories (PFCs) and Component Material Category (CMC) of EU fertilizing products, as described in the Regulation (EU) 2019/1009 of the European Parliament and of the Council [1]:
—   PFC 6(A): Microbial plant biostimulant;
—   PFC 6(B): Non-microbial plant biostimulant;
—   CMC 7: Microorganisms.
It requires three successive steps: a selective enrichment, an isolation on a chromogenic agar, and if positive a confirmation with a serological test (and if required, a selective media).

  • Technical specification
    19 pages
    English language
    sale 10% off
    e-Library read for
    1 day
  • Draft
    21 pages
    English language
    sale 10% off
    e-Library read for
    1 day

This document provides a method for the detection of Listeria monocytogenes in microbial plant biostimulants for verifying that the concentration of this human pathogen does not exceed the respective limits outlined in the EU Regulation on Fertilising Products [1].

  • Technical specification
    25 pages
    English language
    sale 10% off
    e-Library read for
    1 day
  • Draft
    27 pages
    English language
    sale 10% off
    e-Library read for
    1 day

This methodology has been developed to determine enterococci in biostimulants as a single microorganism component or in a mixture with other microorganisms. This document is not applicable to mineral fertilizers that are defined as complementary feeding stuffs composed mainly of minerals and containing at least 40 % crude ash (Regulation (EC) No 767/2009) [3].

  • Technical specification
    14 pages
    English language
    sale 10% off
    e-Library read for
    1 day
  • Draft
    14 pages
    English language
    sale 10% off
    e-Library read for
    1 day

This document provides the methodology for the enumeration and determination of Azospirillum spp. in plant biostimulant products in accordance to the Regulation of EU fertilising products [1].

  • Technical specification
    15 pages
    English language
    sale 10% off
    e-Library read for
    1 day
  • Draft
    15 pages
    English language
    sale 10% off
    e-Library read for
    1 day

This document specifies a horizontal method for the detection of enteropathogenic Vibrio spp., which causes human illness in or via the intestinal tract [1]. The species detectable by the methods specified include Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus.
It is applicable to the following:
—   microbial plant biostimulants.
NOTE 1   The World Health Organization (WHO) has identified that V. parahaemolyticus, V. cholerae and V. vulnificus are the major contaminants of Vibrio spp. [1].
NOTE 2   For confirmation, it is possible to use PCR tests; in this case the laboratory validates the procedure and data generated.

  • Technical specification
    33 pages
    English language
    sale 10% off
    e-Library read for
    1 day
  • Draft
    36 pages
    English language
    sale 10% off
    e-Library read for
    1 day

This document was developed to provide the methodology for the enumeration and determination of Azotobacter sp. in plant biostimulant products in accordance with the Regulation (EU) 2019/1009 of the European Parliament and of the Council [1].

  • Technical specification
    13 pages
    English language
    sale 10% off
    e-Library read for
    1 day
  • Draft
    13 pages
    English language
    sale 10% off
    e-Library read for
    1 day

This document defines general rules for the aerobic preparation of the initial suspension and of dilutions for microbiological examinations of microbial plant biostimulants.
This horizontal method might not be appropriate in very detail for certain products. In this case, different methods which are specific to these products can be used if necessary, for justified technical reasons.

  • Technical specification
    17 pages
    English language
    sale 10% off
    e-Library read for
    1 day
  • Draft
    18 pages
    English language
    sale 10% off
    e-Library read for
    1 day

This document provides the methodology for the enumeration and determination of Rhizobium sp., Mesorhizobium sp., Ensifer sp., or Bradyrhizobium sp. in plant biostimulant products in accordance with Regulation (EU) 2019/1009 of the European Parliament and of the Council [1].

  • Draft
    22 pages
    English language
    sale 10% off
    e-Library read for
    1 day

This document describes a method for the detection of Salmonella spp. in biostimulants of the following Product Function Categories (PFCs) and Component Material Category (CMC) of EU fertilizing products, as described in Regulation (EU) 2019/1009 of the European Parliament and of the Council [1]:
-   PFC 6(A): Microbial plant biostimulant;
-   PFC 6(B): Non-microbial plant biostimulant;
-   CMC 7: Microorganisms.
It requires three successive steps: a selective enrichment, an isolation on a chromogenic agar, and if positive a confirmation with a serological test (and if required, a selective media).

  • Draft
    24 pages
    English language
    sale 10% off
    e-Library read for
    1 day

This methodology has been developed to determine enterococci in biostimulants as a single microorganism component or in a mixture with other microorganisms. This document is not applicable to mineral fertilizers that are defined as complementary feeding stuffs composed mainly of minerals and containing at least 40 % crude ash (Council Directive 79/373/EEC) [3].

  • Draft
    18 pages
    English language
    sale 10% off
    e-Library read for
    1 day

This document specifies a horizontal method for the detection of enteropathogenic Vibrio spp., which causes human illness in or via the intestinal tract [1]. The species detectable by the methods specified include Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus.
It is applicable to the following:
-   microbial plant biostimulants.
NOTE 1   The World Health Organization (WHO) has identified that V. parahaemolyticus, V. cholerae and V. vulnificus are the major contaminants of Vibrio spp. [1].
NOTE 2   For confirmation, it is possible to use PCR tests; in this case the laboratory must validate the procedure and data generated.

  • Draft
    41 pages
    English language
    sale 10% off
    e-Library read for
    1 day

This document specifies a horizontal method for the enumeration of yeasts and moulds present in plant biostimulants intended for use in agriculture, by means of the colony count technique after aerobic incubation at 25 °C ± 2,5 °C.
This document allows the enumeration of yeasts and moulds, in technical and formulated plant biostimulant, both in liquid and solid state. The method is applicable to microbial plant biostimulant except those composed of fungi or yeast to verify that the concentration of yeast and moulds does not exceed the respective limits described in the EU Fertilisers Regulation [1].
If necessary, yeast and mould enumerated can be identified using suitable identification tests.

  • Draft
    23 pages
    English language
    sale 10% off
    e-Library read for
    1 day

This document provides a method for verifying that the pathogen Staphylococcus aureus is absent from microbial plant biostimulants according to the limits outlined in the EU Regulation on Fertilising Products [2].

  • Draft
    18 pages
    English language
    sale 10% off
    e-Library read for
    1 day

This document defines general rules for the aerobic preparation of the initial suspension and of dilutions for microbiological examinations of microbial plant biostimulants.
This horizontal method might not be appropriate in very detail for certain products. In this case, different methods which are specific to these products can be used if necessary, for justified technical reasons.

  • Draft
    19 pages
    English language
    sale 10% off
    e-Library read for
    1 day

This document specifies general rules to determine the concentration of microorganisms present in plant biostimulants.
The method is applicable to microbial plant biostimulants for verifying that the concentration of microorganisms does not exceed the respective limits outlined in the EU Regulation on Fertilising Products [1].
This horizontal method might not be appropriate in every detail for certain products. In this case, it is necessary to refer to the methodology of specific determination and quantification of the microorganisms.

  • Draft
    21 pages
    English language
    sale 10% off
    e-Library read for
    1 day

This document gives general guidelines for the detection and identification of the specified microorganism Escherichia coli in technical and formulated biostimulant products, both in liquid and solid state, and also the horizontal method for the enumeration of ß-glucuronidase-positive Escherichia coli in plant biostimulants products (both in liquid and solid state).
The qualitative method described in this document is based on the detection of Escherichia coli in a non-selective liquid medium (enrichment broth), followed by isolation on a selective agar medium. Other methods can be appropriate, depending on the level of detection required.
NOTE   For the detection of Escherichia coli, subcultures can be performed on non-selective culture media followed by suitable identification steps (e.g. using identification kits).
The quantitative method described in this document uses a colony-count technique at 44 °C on a solid medium containing a chromogenic ingredient for detection of the enzyme ß-glucuronidase.
WARNING - Strains of Escherichia coli which do not grow at 44 °C and, in particular, those that are ß-glucuronidase negative, such as Escherichia coli O157, will not be detected.

  • Draft
    25 pages
    English language
    sale 10% off
    e-Library read for
    1 day

This standard was developed to provide a horizontal method for enumeration and genera/species determination [1], [2], [3] of mycorrhizal fungi in microbial plant biostimulant in accordance with the Regulation (EU) 2019/1009 of the European Parliament and of the Council.

  • Draft
    45 pages
    English language
    sale 10% off
    e-Library read for
    1 day

This document provides a horizontal method for enumeration of microorganisms that are able to grow and form colonies in a solid medium after anaerobic incubation at 30 °C.
The method is applicable to microbial plant biostimulants, except those composed of aerobic bacteria, for verifying that the concentration of anaerobes does not exceed the respective limits outlined in the EU Fertilizers Regulation [1].
This method does not apply to the microbiological monitoring of the environment in which microbial plant biostimulants are manufactured.
No information about potential human pathogens can be inferred from anaerobic plate counts.

  • Draft
    18 pages
    English language
    sale 10% off
    e-Library read for
    1 day

This document provides a method for verifying that the pathogen Shigella spp. is not present in microbial plant biostimulants in a concentration that exceeds the respective limits outlined in the EU Regulation on Fertilising Products.
The detection method for Shigella pathogens is not sensitive and quantification is rarely performed. Detection is usually performed using an enrichment medium followed by subculturing onto a variety of selective media.

  • Draft
    32 pages
    English language
    sale 10% off
    e-Library read for
    1 day

This document provides the methodology for the enumeration and determination of Azospirillum spp. in Plant Biostimulant products in accordance with the Regulation (EU) 2019/1009 of the European Parliament and of the Council  [1].

  • Draft
    22 pages
    English language
    sale 10% off
    e-Library read for
    1 day

This document specifies a method for laboratory measurement of the pH value in liquid microbial plant biostimulants, using pH electrodes with a glass membrane.
Plant biostimulants other than microbial plant biostimulants are excluded from the scope of this document because there is no essential requirement in the Regulation [1] for measuring the pH of non-microbial plant biostimulants.

  • Draft
    18 pages
    English language
    sale 10% off
    e-Library read for
    1 day

This document provides a method for the detection of Listeria monocytogenes in microbial plant biostimulants for verifying that the content of this human pathogen agrees with the respective limits outlined in the EU Regulation on Fertilising Products [1].

  • Draft
    32 pages
    English language
    sale 10% off
    e-Library read for
    1 day

This document was developed to provide the methodology for the enumeration and determination of Azotobacter sp. [2] [3] in microbial plant biostimulants in accordance with the Regulation (EU) 2019/1009 of the European Parliament and of the Council [1].

  • Draft
    19 pages
    English language
    sale 10% off
    e-Library read for
    1 day