Plant biostimulants - Detection of Salmonella spp.

This document describes a method for the detection of Salmonella spp. in biostimulants of the following Product Function Categories (PFCs) and Component Material Category (CMC) of EU fertilizing products, as described in the Regulation (EU) 2019/1009 of the European Parliament and of the Council [1]:
—   PFC 6(A): Microbial plant biostimulant;
—   PFC 6(B): Non-microbial plant biostimulant;
—   CMC 7: Microorganisms.
It requires three successive steps: a selective enrichment, an isolation on a chromogenic agar, and if positive a confirmation with a serological test (and if required, a selective media).

Pflanzen-Biostimulanzien - Nachweis von Salmonella spp.

Dieses Dokument beschreibt ein Verfahren zum Nachweis von Salmonella spp. in Biostimulanzien der folgenden Produktfunktionskategorien (PFCs, en: Product Function Categories) und Komponenten-materialkategorien (CMC, en: Component Material Category) von EU Düngeprodukten, wie in der Verordnung (EU) 2019/1009 des Europäischen Parlaments und des Rates [1] beschrieben:
—   PFC 6(A): Mikrobielles Pflanzen-Biostimulans;
—   PFC 6(B): Nicht-mikrobielles Pflanzen-Biostimulans;
—   CMC 7: Mikroorganismen.
Es erfordert drei aufeinanderfolgende Schritte: eine selektive Anreicherung, eine Isolierung auf einem chromogenen Agar und bei positivem Befund eine Bestätigung mit einer serologischen Untersuchung (und, falls erforderlich, einem Selektivmedium).

Biostimulants des végétaux - Détection de Salmonella spp.

Le présent document décrit une méthode pour la recherche des Salmonella spp. dans les biostimulants des catégories fonctionnelles de produits (PFC) et de la catégorie de matières constitutives (CMC) des fertilisants UE décrites dans le règlement (EU) 2019/1009 du Parlement européen et du Conseil [1] :
- PFC 6(A) : Biostimulant microbien des végétaux ;
- PFC 6(B) : Biostimulant non-microbien des végétaux ;
- CMC 7 : Microorganismes.
Elle nécessite trois phases successives : un enrichissement sélectif, un isolement sur un milieu gélosé chromogène, et, en cas de résultat positif, une confirmation par un essai sérologique (et, si nécessaire, un milieu sélectif).

Rastlinski biostimulansi - Ugotavljanje prisotnosti salmonele (Salmonella spp.)

Ta dokument opisuje metodo za ugotavljanje prisotnosti Salmonella spp. v biostimulantih naslednjih funkcijskih kategorij proizvodov (PFC) in kategorij sestavnih materialov (CMC) v sredstvih za gnojenje EU, kot je opisano v Uredbi (EU) 2019/1009 Evropskega parlamenta in Sveta [1]:
–   PFC 6(A): mikrobni rastlinski biostimulanti;
–   PFC 6(B): nemikrobni rastlinski biostimulanti;
–   CMC 7: mikroorganizmi.
Metoda zahteva tri zaporedne korake: neselektivno obogatitev, izolacijo na kromogenem agarju in, če je rezultat pozitiven, potrditev s serološkim preskusom (in po potrebi s selektivnim gojiščem).

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Technical specification
TS CEN/TS 17717:2023
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Standards Content (Sample)

SIST-TS CEN/TS 17717:2023
Rastlinski biostimulansi - Ugotavljanje prisotnosti salmonele (Salmonella spp.)
Plant biostimulants - Detection of Salmonella spp
Pflanzen-Biostimulanzien - Nachweis von Salmonella spp.
Biostimulants des végétaux - Détection de Salmonella spp.
Ta slovenski standard je istoveten z: CEN/TS 17717:2022
65.080 Gnojila Fertilizers
SIST-TS CEN/TS 17717:2023 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST-TS CEN/TS 17717:2023

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SIST-TS CEN/TS 17717:2023

CEN/TS 17717


March 2022
ICS 65.080
English Version

Plant biostimulants - Detection of Salmonella spp.
Biostimulants des végétaux - Détection de Salmonella Pflanzen-Biostimulanzien - Nachweis von Salmonella
spp. spp.
This Technical Specification (CEN/TS) was approved by CEN on 3 January 2022 for provisional application.

The period of validity of this CEN/TS is limited initially to three years. After two years the members of CEN will be requested to
submit their comments, particularly on the question whether the CEN/TS can be converted into a European Standard.

CEN members are required to announce the existence of this CEN/TS in the same way as for an EN and to make the CEN/TS
available promptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in
parallel to the CEN/TS) until the final decision about the possible conversion of the CEN/TS into an EN is reached.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.



CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2022 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN/TS 17717:2022 E
worldwide for CEN national Members.

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SIST-TS CEN/TS 17717:2023
CEN/TS 17717:2022 (E)
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 6
4.1  General . 6
4.2  Enrichment in selective liquid medium . 6
4.3  Plating out on selective solid media . 6
4.4  Confirmation . 6
5 Culture media, reagents, antisera . 6
5.1  General . 6
5.2  Isolation chromogenic agar . 7
5.3  Non-selective agar . 7
5.4  Confirmation selective agar . 7
6 Equipment and consumables .

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