Plant biostimulants - Detection of Shigella spp.

This document provides a method for verifying that the pathogen Shigella spp. is not present in microbial plant biostimulants in a concentration that exceeds the respective limits outlined in the EU Regulation on Fertilising Products.
The detection method for Shigella pathogens is not sensitive and quantification is rarely performed. Detection is usually performed using an enrichment medium followed by subculturing onto a variety of selective media.

Pflanzen-Biostimulanzien - Nachweis von Shigella spp.

Dieses Dokument liefert ein Verfahren zum Nachweis, dass das Pathogen Shigella spp. in mikrobiellen Pflanzen-Biostimulanzien nicht in einer Konzentration vorhanden ist, die die jeweiligen Grenzwerte überschreitet, die in der EU-Düngeprodukteverordnung festgelegt sind.
Das Nachweisverfahren für Shigella-Pathogene ist nicht trennscharf, und eine Quantifizierung wird selten durchgeführt. Der Nachweis erfolgt in der Regel mit einem Anreicherungsmedium und anschließender Subkultivierung auf verschiedenen Selektivmedien.

Biostimulants des végétaux - Recherche de Shigella spp.

Le présent document fournit une méthode permettant de vérifier que le pathogène Shigella spp n’est pas présent dans les biostimulants microbiens des végétaux à une concentration dépassant les limites correspondantes indiquées dans le Règlement UE sur les fertilisants.
La méthode de détection des pathogènes Shigella n’est pas sensible et la quantification est rarement réalisée. La détection est généralement réalisée en utilisant un milieu d’enrichissement, puis en procédant à un repiquage sur une variété de milieux sélectifs.

Rastlinski biostimulanti - Ugotavljanje prisotnosti Shigella spp.

Ta dokument zagotavlja metodo za preverjanje, da patogen Shigella spp. ni prisoten v mikrobnih rastlinskih biostimulantih v koncentraciji, ki presega ustrezne omejitve, določene v Uredbi EU o sredstvih za gnojenje.
Metoda ugotavljanja prisotnosti patogenov Shigella ni občutljiva in kvantifikacija se redko izvaja. Prisotnost patogenov se običajno ugotavlja z uporabo obogatitvenega gojišča, ki mu sledi subkultura na različnih selektivnih gojiščih.

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SIST-TS CEN/TS 17715:2023
Rastlinski biostimulanti - Ugotavljanje prisotnosti Shigella spp.
Plant biostimulants - Detection of Shigella spp
Pflanzen-Biostimulanzien - Nachweis von Shigella spp.
Biostimulants des végétaux - Recherche de Shigella spp.
Ta slovenski standard je istoveten z: CEN/TS 17715:2022
65.080 Gnojila Fertilizers
SIST-TS CEN/TS 17715:2023 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST-TS CEN/TS 17715:2023

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SIST-TS CEN/TS 17715:2023

CEN/TS 17715


March 2022
ICS 65.080
English Version

Plant biostimulants - Detection of Shigella spp.
Biostimulants des végétaux - Recherche de Shigella Pflanzen-Biostimulanzien - Nachweis von Shigella spp.
This Technical Specification (CEN/TS) was approved by CEN on 3 January 2022 for provisional application.

The period of validity of this CEN/TS is limited initially to three years. After two years the members of CEN will be requested to
submit their comments, particularly on the question whether the CEN/TS can be converted into a European Standard.

CEN members are required to announce the existence of this CEN/TS in the same way as for an EN and to make the CEN/TS
available promptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in
parallel to the CEN/TS) until the final decision about the possible conversion of the CEN/TS into an EN is reached.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.



CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2022 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN/TS 17715:2022 E
worldwide for CEN national Members.

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SIST-TS CEN/TS 17715:2023
CEN/TS 17715:2022 (E)
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 5
4.1 General. 5
4.2 Enrichment in selective liquid medium . 5
4.3 Plating out and identification of colonies . 5
4.4 Biochemical and serological confirmation . 6
5 Culture media, reagents and antisera . 6
6 Apparatus and glassware . 6
7 Sampling . 6
8 Procedure . 6
8.1 General.

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