FprEN 17701-2
(Main)Plant biostimulants - Determination of specific elements - Part 2: Determination of total content of Cd, Pb, Ni, As, Cr, Cu and Zn
Plant biostimulants - Determination of specific elements - Part 2: Determination of total content of Cd, Pb, Ni, As, Cr, Cu and Zn
This document specifies a method for the determination of total contents of arsenic (As), cadmium (Cd), copper (Cu), chromium (Cr), lead (Pb), nickel (Ni) and zinc (Zn) in aqua regia plant biostimulant digests using inductively coupled plasma-atomic emission spectrometry (ICP-AES).
This document is applicable to the blends of fertilizing products where a blend is a mix of at least two of the following component EU fertilizing products categories: Fertilizers, Liming Materials, Soil Improvers, Growing Media, Inhibitors, Plant Biostimulants, and where the following category Plant Biostimulants is the highest % in the blend by mass or volume, or in the case of liquid form by dry mass. If Plant Biostimulants is not the highest % in the blend, the European Standard for the highest % of the blend applies. In case a blend of fertilizing products is composed of components in equal quantity or in case the component EU fertilising products used for the blend have identical formulations, the user decides which standard to apply.
This method is applicable to aqua regia digests prepared according to EN 17701-1:—1. The method can be used for the determination of other elements, provided the user has verified the applicability.
NOTE Alternatively, inductively coupled plasma mass spectrometry (ICP-MS) can be used for the determination of the elements in the aqua regia digests if the user proves that the method gives the same results.
Pflanzen-Biostimulanzien - Bestimmung spezifischer Elemente - Teil 2: Bestimmung des Gesamtgehaltes an Cd, Pb, Ni, As, Cr, Cu und Zn
Dieses Dokument legt ein Verfahren zur Bestimmung des Gesamtgehalts an Arsen (As), Cadmium (Cd), Kupfer (Cu), Chrom (Cr), Blei (Pb), Nickel (Ni) und Zink (Zn) in Pflanzen-Biostimulanzien mit Atomemissions¬spektrometrie mit induktiv gekoppeltem Plasma (ICP AES) nach Königswasseraufschluss fest.
Dieses Dokument ist für die Mischungen von Düngeprodukten anwendbar, in denen eine Mischung aus mindestens zwei der folgenden Kategorien von Komponenten-EU Düngeprodukten besteht: Düngemittel, Kalkdünger, Bodenverbesserungsmittel, Kultursubstrate, Hemmstoffe, Pflanzen-Biostimulanzien und wenn die folgende Kategorie Pflanzen-Biostimulanzien den höchsten Massen- oder Volumenanteil in der Mischung bzw., bei flüssiger Form, den höchsten Trockenmasseanteil aufweist. Wenn Pflanzen-Biostimulanzien nicht den höchsten prozentualen Anteil in der Mischung ausmachen, ist die für den höchsten prozentualen Anteil geltende Europäische Norm anwendbar. Falls eine Düngemittelmischung aus Komponenten gleicher Menge besteht oder falls die für die Mischung verwendeten Komponenten-EU Düngeprodukte identische Formulierungen haben, entscheidet der Anwender, welche Norm angewendet wird.
Das Verfahren ist für Königswasseraufschlüsse anwendbar, die nach EN 17701 1:—1 hergestellt wurden. Das Verfahren kann zur Bestimmung anderer Elemente angewendet werden, vorausgesetzt der Anwender hat die Anwendbarkeit überprüft.
ANMERKUNG Alternativ kann die Massenspektrometrie mit induktiv gekoppeltem Plasma (ICP MS) für die Bestimmung der Elemente in den Königswasser-Aufschlüssen genutzt werden, sofern der Anwender nachweist, dass das Verfahren zu den gleichen Ergebnissen führt.
Biostimulants des végétaux - Dosage des éléments spécifiques - Partie 2 : Détermination de la teneur totale en Cd, Pb, Ni, As, Cr, Cu et Zn
Le présent document spécifie une méthode pour le dosage des teneurs totales de l’arsenic (As), du cadmium (Cd), du cuivre (Cu), du chrome (Cr), du plomb (Pb), du nickel (Ni) et du zinc (Zn) dans les digestats de biostimulants des végétaux à l’eau régale par spectrométrie d’émission atomique par un plasma à couplage inductif (ICP-AES).
Le présent document est applicable aux mélanges de fertilisants constitués d’au moins deux composants appartenant aux catégories de fertilisants UE suivantes : engrais, amendements minéraux basiques, amendements du sol, supports de culture, inhibiteurs, biostimulants des végétaux et, dans lesquels la catégorie Biostimulants des végétaux représente le % le plus élevé du mélange en masse ou en volume, ou dans le cas d’un liquide, en masse sèche. Si les biostimulants des végétaux ne représentent pas le % le plus élevé du mélange, la Norme européenne relative au composant représentant le % le plus élevé du mélange s’applique. Dans le cas où un mélange de fertilisants contient des composants en quantité égale ou dans le cas où les composants des fertilisants UE utilisés pour le mélange ont des formulations identiques, l’utilisateur décide quelle norme appliquer.
Cette méthode est applicable aux digestats à l’eau régale préparés conformément à l’EN 17701 1:—1. La méthode peut également être utilisée pour le dosage d’autres éléments, à condition que l’utilisateur ait vérifié son applicabilité.
NOTE Il est également possible d’utiliser la spectrométrie de masse avec plasma à couplage inductif (ICP-MS) pour le dosage des éléments dans les digestats à l’eau régale, si l’utilisateur prouve que la méthode conduit aux mêmes résultats.
Rastlinski biostimulanti - Določanje specifičnih elementov - 2. del: Določanje celotnega Cd, Pb, Ni, As, Cr, Cu in Zn
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
oSIST prEN 17701-2:2023
01-maj-2023
Rastlinski biostimulanti - Določevanje specifičnih elementov - 2. del: Določevanje
celotnega Cd, Pb, Ni, As, Cr, Cu in Zn
Plant biostimulants - Determination of specific elements - Part 2: Determination of total
content of Cd, Pb, Ni, As, Cr, Cu and Zn
Biostimulanzien für die pflanzliche Anwendung - Bestimmung spezifischer Elemente -
Teil 2: Bestimmung des Gesamtgehaltes an Cd, Pb, Ni, As, Cr, Cu und Zn
Biostimulants des végétaux - Détermination des éléments spécifiques - Partie 2 :
Détermination du contenu total en Cd, Pb, Ni, As, Cr, Cu et Zn
Ta slovenski standard je istoveten z: prEN 17701-2
ICS:
65.080 Gnojila Fertilizers
oSIST prEN 17701-2:2023 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
oSIST prEN 17701-2:2023
oSIST prEN 17701-2:2023
DRAFT
EUROPEAN STANDARD
prEN 17701-2
NORME EUROPÉENNE
EUROPÄISCHE NORM
March 2023
ICS 65.080 Will supersede CEN/TS 17701-2:2022
English Version
Plant biostimulants - Determination of specific elements -
Part 2: Determination of total content of Cd, Pb, Ni, As, Cr,
Cu and Zn
Biostimulants des végétaux - Détermination des Biostimulanzien für die pflanzliche Anwendung -
éléments spécifiques - Partie 2 : Détermination du Bestimmung spezifischer Elemente - Teil 2:
contenu total en Cd, Pb, Ni, As, Cr, Cu et Zn Bestimmung des Gesamtgehaltes an Cd, Pb, Ni, As, Cr,
Cu und Zn
This draft European Standard is submitted to CEN members for enquiry. It has been drawn up by the Technical Committee
CEN/TC 455.
If this draft becomes a European Standard, CEN members are bound to comply with the CEN/CENELEC Internal Regulations
which stipulate the conditions for giving this European Standard the status of a national standard without any alteration.
This draft European Standard was established by CEN in three official versions (English, French, German). A version in any other
language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC
Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.
Warning : This document is not a European Standard. It is distributed for review and comments. It is subject to change without
notice and shall not be referred to as a European Standard.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2023 CEN All rights of exploitation in any form and by any means reserved Ref. No. prEN 17701-2:2023 E
worldwide for CEN national Members.
oSIST prEN 17701-2:2023
prEN 17701-2:2023 (E)
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 6
5 Interferences . 6
5.1 General. 6
5.2 Spectral interferences . 6
5.3 Transport interferences . 6
5.4 Excitation interferences . 7
5.5 Chemical interferences . 7
5.6 Memory interferences . 7
6 Reagents . 7
7 Apparatus . 8
8 Procedure . 8
8.1 Preparation of test sample solution and blank test solution . 8
8.2 Preparation of the calibration solutions . 9
8.3 Measurement . 9
8.3.1 Instrument conditions . 9
8.3.2 Optimization of the instrument conditions . 9
8.3.3 Interferences . 9
8.3.4 Measurement . 10
8.3.5 Matrix effects . 10
8.3.6 Spiking . 10
9 Calculation and expression of the results . 12
10 Test report . 12
Annex A (informative) Results of the inter-laboratory study . 13
A.1 Inter-laboratory tests . 13
A.2 Statistical results for the determination of elements . 13
Annex ZA (informative) Relationship of this European Standard and the essential
requirements of Regulation (EU) 2019/1009 making available on the market of EU
fertilising products aimed to be covered . 21
Bibliography . 22
oSIST prEN 17701-2:2023
prEN 17701-2:2023 (E)
European foreword
This document (prEN 17701-2:2023) has been prepared by Technical Committee CEN/TC 455 “Plant
biostimulants”, the secretariat of which is held by AFNOR.
This document is currently submitted to the CEN Enquiry.
This document will supersede CEN/TS 17701-2:2022.
This document has been prepared under a Standardization Request given to CEN by the European
Commission and the European Free Trade Association, and supports essential requirements of EU
Directive(s) / Regulation(s).
For relationship with EU Directive(s) / Regulation(s), see informative Annex ZA, which is an integral part
of this document.
EN 17701 series, Plant biostimulants — Determination of specific elements, consists of the following parts:
— Part 1: Digestion by aqua regia for subsequent determination of elements;
— Part 2: Determination of total content of Cd, Pb, Ni, As, Cr, Cu and Zn;
— Part 3: Determination of mercury.
oSIST prEN 17701-2:2023
prEN 17701-2:2023 (E)
Introduction
This document was prepared by the experts of CEN/TC 455 “Plant biostimulants”. The European
Committee for Standardization (CEN) was requested by the European Commission (EC) to draft
European standards or European standardization deliverables to support the implementation of
Regulation (EU) 2019/1009 of the European Parliament and of the Council of 5 June 2019 laying down
rules on the making available on the market of EU fertilising products (“FPR” or “Fertilising Products
Regulation”).
This standardization request, presented as M/564 and M/564/Amd1, also contributes to the
Communication on “Innovating for Sustainable Growth: A Bio economy for Europe”. Working Group 4
“Other safety parameters” was created to develop a work programme as part of this standardization
request. Technical Committee CEN/TC 455 “Plant biostimulants” was established to carry out the work
programme that will prepare a series of standards. The interest in biostimulants has increased
significantly in Europe as a valuable tool to use in agriculture. Standardization was identified as having
an important role in order to promote the use of biostimulants. The work of CEN/TC 455 seeks to improve
the reliability of the supply chain, thereby boosting the confidence of farmers, industry, and consumers
in biostimulants, and will promote and support commercialisation of the European biostimulant
industry.
The preparation of this document is based on a standardization request to CEN by the European
Commission and the European Free Trade Association (Mandate M/564) concerning the modernization
of methods of analysis of fertilizers in the framework of Regulation (EU) 2019/1009 of the European
Parliament and of the Council.
This document is part of a modular approach and concerns the analytical measurement step. “Modular”
means that a test standard concerns a specific step in assessing a property and not the whole chain of
measurements. Aqua regia digestion followed by inductively coupled plasma atomic emission
spectrometry is widely used for determination of many elements. For example, a multi-matrix standard
for aqua regia extraction of soils, sludges and biowaste was prepared by CEN/TC 444. A similar procedure
was applied for determination of aqua regia extractable contents of arsenic, mercury, cadmium,
chromium, nickel and lead in fertilizers and liming materials (standard prepared by CEN/TC 260).
Inductively coupled plasma atomic emission spectrometry (ICP-AES) is nowadays widely used and
a well-established method in many laboratories.
The inter-laboratory study reflects the final statistical characteristics of the method for the determination
of the specific elements in aqua regia digests including both, the digestion and the measurement steps.
WARNING — Persons using this document should be familiar with usual laboratory practice. This
document does not purport to address all of the safety issues, if any, associated with its use. It is the
responsibility of the user to establish appropriate health and safety practices and to ensure compliance
with any national regulatory conditions.
IMPORTANT — It is absolutely essential that tests conducted according to this document are carried out
by suitably trained staff.
oSIST prEN 17701-2:2023
prEN 17701-2:2023 (E)
1 Scope
This document specifies a method for the determination of arsenic (As), cadmium (Cd), copper (Cu),
chromium (Cr), lead (Pb), nickel (Ni) and zinc (Zn) in aqua regia plant biostimulant digests using
inductively coupled plasma-atomic emission spectrometry (ICP-AES).
This document is applicable to the fertilizing product blends belonging to PFC 7 where the EU fertilising
product plant biostimulant contained in the blend represents the highest % by mass in the blend. In case
of equal shares, the user can apply either this or the standard(s) applicable to the other component
product(s).
This method is applicable to aqua regia digests prepared according to EN 17701-1:— . The method can
be used for the determination of other elements, provided the user has verified the applicability.
NOTE Alternatively, inductively coupled plasma mass spectrometry (ICP-MS) can be used for the
determination of the elements in the aqua regia digests if the user proves that the method gives the same results.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
EN 17701-1:— , Plant biostimulants — Determination of specific elements — Part 1: Digestion by aqua
regia for subsequent determination of elements
EN 17704:— , Plant biostimulants — Determination of dry matter
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
analyte
parameter to be determined
3.2
blank calibration solution
solution prepared in the same way as the calibration solution but leaving out the analytes
3.3
blank test solution
solution prepared in the same way as the test sample solution b
...
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