EN 17707:2024
(Main)Plant biostimulants - Determination of the yeast and mould content
Plant biostimulants - Determination of the yeast and mould content
This document specifies a horizontal method for the enumeration of yeasts and moulds present in plant biostimulants intended for use in agriculture, by means of the colony count technique after aerobic incubation at 25 °C ± 2,5 °C.
This document allows the enumeration of yeasts and moulds, in technical and formulated plant biostimulants, both in liquid and solid states. The method is applicable to microbial plant biostimulants except those composed of fungi or yeasts.
If necessary, yeasts and moulds enumerated can be identified using suitable identification tests.
This document is applicable to the blends of fertilizing products where a blend is a mix of at least two of the following component EU fertilising products categories: Fertilizers, Liming Materials, Soil Improvers, Growing Media, Plant Biostimulants, and where the following category Plant Biostimulants is the highest % in the blend by mass or volume, or in the case of liquid form by dry mass. If Plant Biostimulants is not the highest % in the blend, the European Standard for the highest % of the blend applies. In case a blend of fertilizing products is composed of components in equal quantity or in case the component EU fertilising products used for the blend have identical formulations , the user decides which standard to apply.
Pflanzen-Biostimulanzien - Bestimmung des Gehalts an Hefen und Schimmelpilzen
Dieses Dokument legt ein horizontales Verfahren zur Zählung von Hefen und Schimmelpilzen in Pflanzen-Biostimulanzien, die zur Verwendung in der Landwirtschaft bestimmt sind, mithilfe des Koloniezähl-verfahrens nach aerober Bebrütung bei (25 ± 2,5) °C fest.
Dieses Dokument ermöglicht die Zählung von Hefen und Schimmelpilzen in technischen und formulierten Pflanzen-Biostimulanzien, sowohl in flüssigem als auch in festem Zustand. Das Verfahren ist auf mikrobielle Pflanzen-Biostimulanzien anwendbar, ausgenommen jene, die aus Pilzen oder Hefen bestehen.
Gegebenenfalls können die gezählten Hefen und Schimmelpilze durch geeignete Identifizierungsprüfungen identifiziert werden.
Dieses Dokument ist für die Mischungen von Düngeprodukten anwendbar, in denen eine Mischung aus mindestens zwei der folgenden Kategorien von Komponenten-EU Düngeprodukten besteht: Düngemittel, Kalkdünger, Bodenverbesserungsmittel, Kultursubstrate, Pflanzen-Biostimulanzien und wenn die folgende Kategorie Pflanzen-Biostimulanzien prozentual den höchsten Massen- oder Volumenanteil in der Mischung bzw., bei flüssiger Form, den höchsten Trockenmasseanteil aufweist. Wenn Pflanzen-Biostimulanzien nicht den höchsten prozentualen Anteil in der Mischung ausmachen, ist die für den höchsten prozentualen Anteil geltende Europäische Norm anwendbar. Falls eine Düngemittelmischung aus Komponenten gleicher Menge besteht oder falls die für die Mischung verwendeten Komponenten-EU Düngeprodukte identische Formulierungen haben, entscheidet der Anwender, welche Norm angewendet wird.
Biostimulants des végétaux - Détermination de la teneur en levures et en moisissures
Le présent document spécifie une méthode horizontale pour le dénombrement des levures et des moisissures présentes dans les biostimulants des végétaux destinés à être utilisés en agriculture, par la technique de comptage des colonies après incubation aérobie à 25 °C ± 2,5 °C.
Le présent document permet le dénombrement des levures et des moisissures, dans les produits techniques et formulés à usage biostimulant des végétaux, à la fois à l’état liquide et à l’état solide. La méthode est applicable aux biostimulants microbiens des végétaux, excepté ceux composés de champignons ou de levures.
Si nécessaire, les levures et les moisissures dénombrées peuvent être identifiées par des essais d’identification appropriés.
Le présent document est applicable aux mélanges de fertilisants constitués d’au moins deux composants appartenant aux catégories de fertilisants UE suivantes : engrais, amendements minéraux basiques, amendements du sol, supports de culture, biostimulants des végétaux et, dans lesquels la catégorie Biostimulants des végétaux représente le % le plus élevé du mélange en masse ou en volume, ou dans le cas d’un liquide, en masse sèche. Si les biostimulants des végétaux ne représentent pas le % le plus élevé du mélange, la Norme européenne relative au composant représentant le % le plus élevé du mélange s’applique. Dans le cas où un mélange de fertilisants contient des composants en quantité égale ou dans le cas où les composants des fertilisants UE utilisés pour le mélange ont des formulations identiques, l’utilisateur décide quelle norme appliquer.
Rastlinski biostimulanti - Določanje kvasovk in plesni
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
oSIST prEN 17707:2023
01-maj-2023
Rastlinski biostimulanti - Ugotavljanje prisotnosti in števila kvasovk in plesni
Plant biostimulants - Determination of the yeast and mould content
Pflanzen-Biostimulanzien - Bestimmung des Gehalts an Hefen und Schimmelpilzen
Biostimulants des végétaux - Détermination de la teneur en levures et en moisissures
Ta slovenski standard je istoveten z: prEN 17707
ICS:
65.080 Gnojila Fertilizers
oSIST prEN 17707:2023 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
oSIST prEN 17707:2023
oSIST prEN 17707:2023
DRAFT
EUROPEAN STANDARD
prEN 17707
NORME EUROPÉENNE
EUROPÄISCHE NORM
April 2023
ICS 65.080 Will supersede CEN/TS 17707:2022
English Version
Plant biostimulants - Determination of the yeast and
mould content
Biostimulants des végétaux - Détermination de la Pflanzen-Biostimulanzien - Bestimmung des Gehalts an
teneur en levures et en moisissures Hefen und Schimmelpilzen
This draft European Standard is submitted to CEN members for enquiry. It has been drawn up by the Technical Committee
CEN/TC 455.
If this draft becomes a European Standard, CEN members are bound to comply with the CEN/CENELEC Internal Regulations
which stipulate the conditions for giving this European Standard the status of a national standard without any alteration.
This draft European Standard was established by CEN in three official versions (English, French, German). A version in any other
language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC
Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.
Warning : This document is not a European Standard. It is distributed for review and comments. It is subject to change without
notice and shall not be referred to as a European Standard.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2023 CEN All rights of exploitation in any form and by any means reserved Ref. No. prEN 17707:2023 E
worldwide for CEN national Members.
oSIST prEN 17707:2023
prEN 17707:2023 (E)
Contents Page
European foreword . 4
Introduction . 5
1 Scope . 7
2 Normative references . 7
3 Terms and definitions . 7
4 Principles . 8
4.1 General . 8
4.2 Plate count method . 8
5 Diluent and culture media . 8
5.1 General . 8
5.2 Diluent . 8
5.3 Culture media . 9
6 Apparatus and glassware . 9
7 Handling of plant biostimulants and sampling . 9
8 Procedure . 9
8.1 General . 9
8.2 Test portion and initial suspension . 9
8.2.1 General . 9
8.2.2 Liquid - water based - formulations . 9
8.2.3 Liquid – oil based (emulsifiable concentrate - EC) formulations . 10
8.2.4 Solid - Wettable Powder (WP) formulations . 10
8.2.5 Solid - Water dispersible granules (WDG) formulations . 10
8.2.6 Solid – Pellets, granules, microgranules (slow release) formulations . 10
8.2.7 Solid - substrate . 10
8.3 Serial dilutions . 10
8.4 Plate-count methods . 10
8.4.1 Pour-plate method . 10
8.4.2 Surface spread method . 11
8.4.3 Incubation . 11
9 Counting of colonies . 11
10 Expression of results . 12
10.1 Method of calculation . 12
10.2 Interpretation . 13
11 Test report . 13
Annex A (informative) Diluent . 15
A.1 General . 15
A.2 Phosphate Buffered Saline (PBS). 15
A.2.1 Composition . 15
A.2.2 Preparation . 15
Annex B (normative) Culture media. 16
oSIST prEN 17707:2023
prEN 17707:2023 (E)
B.1 General . 16
B.2 Sabouraud dextrose chloramphenicol agar medium (SDCA) . 16
B.2.1 Composition . 16
B.2.2 Preparation . 16
B.3 Potato dextrose agar (PDA) medium with antibiotics . 17
B.3.1 Composition . 17
B.3.2 Preparation . 17
B.4 Glucose -peptone (GP) agar medium with antibiotics . 17
B.4.1 Composition . 17
B.4.2 Preparation . 17
B.5 Malt extract agar (MEA) medium with antibiotics . 18
B.5.1 Composition . 18
B.5.2 Preparation . 18
Annex C (informative) Interlaboratory study . 19
C.1 Materials used in the interlaboratory study . 19
C.2 Results of the interlaboratory study . 20
Annex ZA (informative) Relationship of this European Standard and the essential
requirements of Regulation (EU) 2019/1009 making available on the market of EU
fertilising products aimed to be covered . 22
Bibliography . 23
oSIST prEN 17707:2023
prEN 17707:2023 (E)
European foreword
This document (prEN 17707:2023) has been prepared by Technical Committee CEN/TC 455
“Plant Biostimulants”, the secretariat of which is held by AFNOR.
This document is currently submitted to the CEN Enquiry.
This document will supersede CEN/TS 17707:2022.
This document has been prepared under a Standardization Request given to CEN by the European
Commission and the European Free Trade Association, and supports essential requirements of EU
Directive(s) / Regulation(s).
For relationship with EU Directive(s) / Regulation(s), see informative Annex ZA, which is an
integral part of this document.
oSIST prEN 17707:2023
prEN 17707:2023 (E)
Introduction
This document was prepared by the experts of CEN/TC 455 “Plant Biostimulants”. The European
Committee for Standardization (CEN) was requested by the European Commission (EC) to draft
European standards or European standardization deliverables to support the implementation of
Regulation (EU) 2019/1009 of 5 June 2019 laying down rules on the making available on the
market of EU fertilizing products (“FPR” or “Fertilising Products Regulation”).
This standardization request, presented as SR M/564 and M/564 Amd1, also contributes to the
Communication on “Innovating for Sustainable Growth: A Bio economy for Europe”. The Working
Group 5 “Labelling and denominations”, was created to develop a work program as part of this
request. The technical committee CEN/TC 455 “Plant Biostimulants” was established to carry out
the work program that will prepare a series of standards. The interest in biostimulants has
increased significantly in Europe as a valuable tool to use in agriculture. Standardization was
identified as having an important role in order to promote the use of biostimulants. The work of
CEN/TC 455 seeks to improve the reliability of the supply chain, thereby improving the confidence
of farmers, industry, and consumers in biostimulants, and will promote and support
commercialisation of the European biostimulant industry.
Biostimulants used in agriculture can be applied in multiple ways: on soil, on plant, as seed
treatment, etc. A microbial plant biostimulant consists of a microorganism or a consortium of
microorganisms, as referred to in Component Material Category 7 of Annex II of the EU Fertilising
Products Regulation.
This document is applicable to all microbial biostimulants in agriculture.
Table 1 below summarizes many of the agro-ecological principles and the role played by
biostimulants.
Table 1 — Agro-ecological principles and the role played by biostimulants [1]
Increase biodiversity
By improving soil microorganism quality/quantity
Reinforce biological regulation and interactions
By reinforcing plant-microorganism interactions
- symbiotic exchanges i.e. mycorrhize
- symbiotic exchanges i.e. rhizobiaciae/fava
- secretions mimicking plant hormones (i.e. trichoderma)
By regulating plant physiological processes
- for ex growth, metabolism, plant development…
Improve bio
...
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