EN ISO 23611-6:2013

Soil quality - Sampling of soil invertebrates - Part 6: Guidance for the design of sampling programmes with soil invertebrates (ISO 23611-6:2012)

This part of ISO 23611 provides guidance for the design of field studies with soil invertebrates (e.g. for the
monitoring of the quality of a soil as a habitat for organisms). Detailed information on the sampling of the
most important soil organisms is provided in the other parts of this International Standard (ISO 23611-1 to
ISO 23611-5).
This part of ISO 23611 is used for all terrestrial biotopes in which soil invertebrates occur. Basic information on
the design of field studies in general is already laid down in ISO 10381-1. This information can vary according
to the national requirements or the climatic/regional conditions of the site to be sampled.
NOTE While this part of ISO 23611 aims to be applicable globally for all terrestrial sites that are inhabited by soil
invertebrates, the existing information refers mostly to temperate regions. However, the (few) studies from other (tropical
and boreal) regions, as well as theoretical considerations, allow the conclusion that the principles laid down in this part of
ISO 23611 are generally valid, References [4], [6], [40], [21].
This part of ISO 23611 gives information on site-specific risk assessment of contaminated land, study of
potential side effects of anthropogenic impacts (e.g. the application of chemicals or the building of roads), the
biological classification and assessment of soils in order to determine the biological quality of soils, and longterm
biogeographical monitoring in the context of nature protection or restoration, including global change (e.g.
as in long-term ecological research projects).

Bodenbeschaffenheit - Probenahme von Wirbellosen im Boden - Teil 6: Anleitung für die Planung der Probenahme von Wirbellosen im Boden (ISO 23611-6:2012)

Dieser Teil von ISO 23611 stellt eine Anleitung zur Planung von Freilanduntersuchungen von Wirbel¬losen im Boden (z. B. zur Überwachung der Qualität eines Bodens als Lebensraum für Organismen) dar Ausführliche Angaben zur Probenahme der am häufigsten auftretenden Bodenorganismen werden in den anderen Teilen dieser Internationalen Norm (ISO 23611 1 bis ISO 23611 5) bereitgestellt.
Dieser Teil von ISO 23611 ist auf alle terrestrischen Biotope anwendbar, in denen bodenbewohnende Wirbellose vorkommen. Grundlegende Informationen zur Planung von Freilanduntersuchungen im Allgemeinen sind bereits in ISO 10381 1 dargelegt. Diese Informationen können in Abhängigkeit von nationalen Anforderungen oder Klimaverhältnissen/regionalen Gegebenheiten des zu beprobenden Standortes variieren.
ANMERKUNG   Obwohl dieser Teil von ISO 23611 global auf alle terrestrischen Standorte anwendbar sein soll, die von Wirbellosen im Boden bewohnt werden, beziehen sich die vorliegenden Angaben meist auf Regionen mit gemäßigtem Klima. Die (wenigen) Untersuchungen von anderen (tropischen und borealen) Regionen sowie theoretische Überlegungen ermöglichen jedoch die Schlussfolgerung, dass die in diesem Teil von ISO 23611 dargelegten Grundsätze allgemeingültig sind [4], [6], [40], [21].
Dieser Teil von ISO 23611 enthält Informationen über die standortspezifische Gefährdungsabschätzung von verunreinigten Flächen, die Untersuchung möglicher Nebenwirkungen von anthropogenen Einflüssen (z. B. der Einsatz von Chemikalien oder der Bau von Straßen), die biologische Klassifizierung und Beurteilung von Böden zur Bestimmung der biologischen Qualität von Böden und über die biogeographische Langzeitbeobachtung im Zusammenhang mit Naturschutz oder Rekultivierungsvorhaben, einschließlich globaler Veränderungen (z. B. wie im Projekt zur ökologischen Langzeitforschung).

Qualité du sol - Prélèvement des invertébrés du sol - Partie 6: Lignes directrices pour la conception de programmes d'échantillonnage des invertébrés du sol (ISO 23611-6:2012)

La présente partie de l'ISO 23611 spécifie les lignes directrices pour la conception de programmes
d'échantillonnage des invertébrés du sol sur le terrain (par exemple la surveillance de la qualité d'un sol
comme habitat pour les organismes). Des informations détaillées sur l'échantillonnage des organismes les
plus importants vivant dans le sol sont fournies dans les autres parties de la présente Norme internationale
(ISO 23611-1 à ISO 23611-5).
La présente partie de l'ISO 23611 est utilisée pour tous les biotopes terrestres dans lesquels se trouvent les
invertébrés du sol. Des informations fondamentales sur la conception des études sur le terrain en général
sont déjà fournies dans l'ISO 10381-1. Ces informations peuvent varier selon les exigences nationales ou les
conditions régionales/climatiques du site à prélever.
NOTE Bien que la présente partie de l'ISO 23611 s'applique globalement à tous les sites terrestres habités par
les invertébrés du sol, les informations existantes se réfèrent principalement aux régions tempérées. Cependant, les
(quelques) études émanant d'autres régions (tropicales et boréales) ainsi que des considérations théoriques permettent
de conclure que les principes établis dans la présente partie de l'ISO 23611 sont généralement valables (Références [4],
[6], [40], [21]).
La présente partie de l'ISO 23611 fournit des informations sur l'évaluation des risques spécifiques à un site
contaminé, l'étude des effets secondaires potentiels des impacts anthropogéniques (par exemple l'utilisation
de produits chimiques ou la construction de routes), la classification et l'évaluation biologiques des sols en
vue de déterminer leur qualité biologique, la surveillance biogéographique à long terme dans le cadre de la
protection ou restauration de la nature, y compris le changement climatique à l'échelle mondiale (par exemple
comme dans les projets de recherche écologique à long terme).

Kakovost tal - Vzorčenje nevretenčarjev v tleh - 6. del: Navodilo za pripravo programov vzorčenja nevretenčarjev v tleh (ISO 23611-6:2012)

Ta del standarda ISO 23611 podaja navodilo za pripravo terenskih raziskav o nevretenčarjih v tleh (npr. za nadzor kakovosti tal kot življenjskega okolja za organizme). Podrobne informacije o vzorčenju najpomembnejših organizmov v tleh so podane v drugih delih tega mednarodnega standarda (od ISO 23611-1 do ISO 23611-5). Ta del standarda ISO 23611 se uporablja za vse zemeljske biotope, v katerih so nevretenčarji. Osnovne informacije o pripravi terenskih raziskav na splošno so že navedene v standardu ISO 10381-1. Te informacije se lahko razlikujejo glede na nacionalne zahteve ali podnebne razmere/regionalne pogoje lokacije, kjer bo potekalo vzorčenje. OPOMBA Namen tega dela standarda ISO 23611 je uporabnost za vse zemeljske lokacije, ki so poseljene z nevretenčarji, vendar obstoječe informacije veljajo predvsem za zmerne regije. Kljub temu je na podlagi raziskav iz drugih (tropskih in borealnih) regij in teoretične ocene mogoče sklepati, da načela v tem delu standarda ISO 23611 v glavnem veljajo (sklici [4], [6], [40], [21]). Ta del standarda ISO 23611 podaja informacije o oceni tveganja onesnažene zemlje, raziskavi o morebitnih stranskih učinkih antropogenih vplivov (npr. uporabe kemikalij ali gradnje cest), biološki klasifikaciji in oceni tal za določevanje biološke kvalitete tal in dolgoročnem biogeografskem nadzoru v kontekstu varovanja in ponovne vzpostavitve naravnega okolja glede na lokacijo, vključno z globalno spremembo (npr. v dolgoročnih ekoloških raziskovalnih projektih).

General Information

• Status: Published
• Publication Date: 30-Jul-2013
• Withdrawal Date: 30-Jan-2014
• ICS: 13.080.05 - Examination of soils in general
• ICS: 13.080.30 - Biological properties of soils
• Technical Committee: CEN/TC 444 - Environmental characterization
• Drafting Committee: CEN/TC 444/WG 4 - Biological characterization
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 31-Jul-2013
• Due Date: 16-Mar-2014
• Completion Date: 31-Jul-2013
• Ref Project: SIST EN ISO 23611-6:2013 - Soil quality - Sampling of soil invertebrates - Part 6: Guidance for the design of sampling programmes with soil invertebrates (ISO 23611-6:2012)

Standards Content (Sample)

SLOVENSKI STANDARD
01-oktober-2013
.DNRYRVWWDO9]RUþHQMHQHYUHWHQþDUMHYYWOHKGHO1DYRGLOR]DSULSUDYR
SURJUDPRYY]RUþHQMDQHYUHWHQþDUMHYYWOHK,62
Soil quality - Sampling of soil invertebrates - Part 6: Guidance for the design of sampling
programmes with soil invertebrates (ISO 23611-6:2012)
Bodenbeschaffenheit - Probenahme von Wirbellosen im Boden - Teil 6: Anleitung für die
Aufstellung von Programmen für die Probenahme von Wirbellosen im Boden (ISO 23611
-6:2012)
Qualité du sol - Prélèvement des invertébrés du sol - Partie 6: Lignes directrices pour la
conception de programmes d'échantillonnage des invertébrés du sol (ISO 23611-6:2012)
Ta slovenski standard je istoveten z: EN ISO 23611-6:2013
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 23611-6
NORME EUROPÉENNE
EUROPÄISCHE NORM
July 2013
ICS 13.080.05; 13.080.30
English Version
Soil quality - Sampling of soil invertebrates - Part 6: Guidance
for the design of sampling programmes with soil invertebrates
(ISO 23611-6:2012)
Qualité du sol - Prélèvement des invertébrés du sol - Partie Bodenbeschaffenheit - Probenahme von Wirbellosen im
6: Lignes directrices pour la conception de programmes Boden - Teil 6: Anleitung für die Planung der Probenahme
d'échantillonnage des invertébrés du sol (ISO 23611- von Wirbellosen im Boden (ISO 23611-6:2012)
6:2012)
This European Standard was approved by CEN on 11 July 2013.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United
Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2013 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 23611-6:2013: E
worldwide for CEN national Members.

Contents Page
Foreword .3

Foreword
The text of ISO 23611-6:2012 has been prepared by Technical Committee ISO/TC 190 “Soil quality” of the
International Organization for Standardization (ISO) and has been taken over as EN ISO 23611-6:2013 by
Technical Committee CEN/TC 345 “Characterization of soils” the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by January 2014, and conflicting national standards shall be withdrawn at
the latest by January 2014.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom.
Endorsement notice
The text of ISO 23611-6:2012 has been approved by CEN as EN ISO 23611-6:2013 without any modification.
INTERNATIONAL ISO
STANDARD 23611-6
First edition
2012-09-15
Soil quality — Sampling of soil
invertebrates —
Part 6:
Guidance for the design of sampling
programmes with soil invertebrates
Qualité du sol — Prélèvement des invertébrés du sol —
Partie 6: Lignes directrices pour la conception de programmes
d’échantillonnage des invertébrés du sol
Reference number
ISO 23611-6:2012(E)
©
ISO 2012
ISO 23611-6:2012(E)
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means,
electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or ISO’s
member body in the country of the requester.
ISO copyright office
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Published in Switzerland
ii © ISO 2012 – All rights reserved

ISO 23611-6:2012(E)
Contents Page
Foreword .iv
Introduction . v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 2
3.1 Soil biology . 2
3.2 Soil protection . 3
3.3 Methods . 4
4 Principle . 4
4.1 General . 4
4.2 Question to be answered when planning a field study . 5
5 Objectives of sampling . 6
5.1 General . 6
5.2 General remarks . 6
5.3 Pre-conditions . 7
5.4 The performance of the site-specific assessment of contaminated land . 7
5.5 The study of potential side effects of anthropogenic impacts . 7
5.6 The biological classification and assessment of soils in order to determine the biological
quality of soils . 7
5.7 Biogeographical monitoring in nature protection or restauration . 8
6 Samples and sampling points . 8
6.1 General . 8
6.2 Sampling patterns . 8
6.3 Selecting and identifying the sampling location . 9
6.4 Preparation of the sampling site . 9
6.5 Further general advice on sampling performance .10
7 Practical considerations for the biological sampling of soils .10
7.1 General .10
7.2 Formal preparations .10
7.3 Requirements on sampling personnel and safety precautions .10
7.4 Preliminary survey . 11
7.5 Main study .12
8 Design options for sampling soil invertebrates .12
8.1 Introduction .12
8.2 Description of possible sampling strategies .13
8.3 Recommendations from the European programme ENVASSO (Environmental Assessment of
Soil for Monitoring) .17
9 Sampling report .19
10 Quality control and quality assurance .20
Annex A (informative) Case studies .21
Bibliography .37
ISO 23611-6:2012(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International
Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 23611-6 was prepared by Technical Committee ISO/TC 190, Soil quality, Subcommittee SC 4,
Biological methods.
ISO 23611 consists of the following parts, under the general title Soil quality — Sampling of soil invertebrates:
— Part 1: Hand-sorting and formalin extraction of earthworms
— Part 2: Sampling and extraction of micro-arthopods (Collembola and Acarina)
— Part 3: Sampling and soil extraction of enchytraeids
— Part 4: Sampling extraction and identification of soil-inhabiting nematodes
— Part 5: Sampling and extraction of soil macro-invertebrates
— Part 6: Guidance for the design of sampling programmes with soil invertebrates
iv © ISO 2012 – All rights reserved
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