This document specifies one of the methods for evaluating the habitat function of soils and determining effects of soil contaminants and individual chemical substances on the reproduction of the oribatid mite Oppia nitens by dermal and alimentary uptake. This chronic (28-day) test is applicable to soils and soil materials of unknown quality (e.g., contaminated sites, amended soils, soils after remediation, agricultural or other sites under concern and waste materials). This method is not intende...view more

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    • Standard
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The present document specifies a method for direct extraction of DNA from soil samples to analyse
the abundance and composition of microbial communities by various techniques of molecular biology
including real-time quantitative PCR (qPCR). This method is mainly dedicated to agricultural and forest
soils. This method can possibly not be suitable for soils rich in organic matter (e.g. peat soils) or soils
heavily polluted with organic pollutants or heavy metals.
The direct extraction of DNA ...
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      1 page
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This document specifies a method for the measurement of several hydrolase activities (arylamidase, arylsulfatase, β-galactosidase, α-glucosidase, β-glucosidase, N-acetyl-glucosaminidase, acid, alkaline and global phosphatases, urease) simultaneously (or not) in soil samples, using colorimetric substrates. Enzyme activities of soil vary seasonally and depend on soil chemical, physical and biological characteristics. This method can be applied either to detect harmful effects on soil enzyme activi...view more

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    • Standard
      37 pages
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The present document specifies a method for direct extraction of DNA from soil samples to analyse the abundance and composition of microbial communities by various techniques of molecular biology including real-time quantitative PCR (qPCR). This method is mainly dedicated to agricultural and forest soils. This method can possibly not be suitable for soils rich in organic matter (e.g. peat soils) or soils heavily polluted with organic pollutants or heavy metals. The direct extraction of DNA from ...view more

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    • Standard
      10 pages
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      10 pages
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      10 pages
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      10 pages
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ISO 17512-2:2011 specifies a rapid screening method for evaluating the habitat function of soils based on the avoidance behaviour of springtails.
The test is a rapid method that reflects the bioavailability of contaminants in natural soils and substances spiked into soils to Folsomia candida. In both cases, it is possible to establish a dose-response-relationship. The avoidance behaviour of the springtails is the measurement endpoint of the test. This test is not intended to replace the Collemb...
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This document describes a method to compare the quality of soils by determining the fatty acid composition of the leaves of plant species grown in these soils.
This method does not make it possible to determine an optimal value of the Omega-3 index and, therefore, cannot be used to determine the intrinsic quality of a soil from a specific area (regarded as homogeneous). The method can only be used to compare the quality of soils between various areas.
This method is applicable to:
— soils fro...
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      31 pages
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This document specifies a chronic test method for evaluating the habitat function of soils and determining effects of soil contaminants and substances on the reproduction of Hypoaspis aculeifer by ? mainly ? alimentary uptake. This method is applicable to soils and soil materials of unknown quality, e.g. from contaminated sites, amended soils, soils after remediation, industrial, agricultural or other sites under concern and waste materials (e.g. dredged material, municipal sludge from a wastewa...view more

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This International Standard gives guidance on the selection and method of appropriate tests for the determination of biodegradation of organic chemicals in soil samples under anaerobic conditions.

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This International Standard provides guidance on the selection and conduct of appropriate test methods for
the determination of biodegradation of organic chemicals in aerobic soils. lt does not describe any specific test method.

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    • Standard
      12 pages
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ISO 17512-1:2008 specifies a rapid screening method for evaluating the habitat function of soils and the influence of contaminants and chemicals on earthworm behaviour.
The sublethal test is a rapid method that reflects the bioavailability of contaminant mixtures in natural soils and substances spiked into soils to Eisenia fetida and Eisenia andrei. The avoidance behaviour of the worms is the measurement endpoint of the test. This test is not intended to replace the earthworm reproduction test....
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ISO 18763:2016 describes a technique for determining the effects of soil and soil-related materials on the seed germination and early growth of higher plants. These endpoints are useful indicators for the assessment of the quality of a soil as a habitat for organisms. It is applicable to all soils in which soil organisms are active and may be used to evaluate:
- the effects on plants due to toxicity of solid or liquid chemicals contaminating soil or materials (compost, sludge, waste) and chemic...
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The purpose of ISO 29200:2013 is to describe a method for assessing genotoxic effects (chromosome breakage or dysfunction of the mitotic spindle) of soils or soil materials on the secondary roots of a higher plant: Vicia faba (broad bean). This method allows the assessment of genotoxicity (toxicity for genetic material) of soils and soil materials like compost, sludge, waste, fertilizing matters, etc. Two ways of exposure can be considered: a direct exposure of plants to the soil (or soil materi...view more

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This International Standard specifies a test method for determining the activity of active aerobic, eterotrophic
microbial biomass in soils. This method is applicable to the monitoring of soil quality and to the evaluation of
the ecotoxic potential of soils and soil materials. It is also applicable for soils sampled along contamination
gradients in the field and to soils that are contaminated experimentally in the field or in the laboratory.

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ISO 14239:2017 specifies six suitable incubation systems for measuring the rates and extent of mineralization of organic compounds in soil by measurement of carbon dioxide (CO2) evolution. All incubation systems are applicable to soluble or insoluble compounds but choice of system depends on the overall purposes of the study.
ISO 14239:2017 does not apply to the use of such systems for material balance studies, which are often test-substance specific.

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This document specifies a protocol to identify ecotoxicological test specimens (mainly invertebrates and plants) to the species level, based on the DNA barcoding technique. This protocol can be used by laboratories performing DNA barcoding in order to standardize both the wet-lab and data analysis workflows as much as possible, and make them compliant with community standards and guidelines.
This document does not intend to specify one particular strain for each test method, but to accurately d...
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This International Standard specifies a rapid method for the determination of the potential rate of ammonium
oxidation and inhibition of nitrification in soils. This method is suitable for all soils containing a population of
nitrifying microorganisms. It can be used as a rapid screening test for monitoring soil quality and quality of
wastes, and is suitable for testing the effects of cultivation methods, chemical substances [except volatiles,
i.e. H > 1 (Henry’s constant)], extracts of bios...
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This document specifies an extraction method to determine the bioavailable (potential and environmental available) fraction and the non-bioavailable fraction of a contaminant in soil using a "receiver phase" for an organic contaminant with strong sorbing or complexing properties, for example, Tenax®[1] or cyclodextrin, respectively. NOTE 1 The bioavailable fraction is defined in ISO 17402 as environmental bioavailability. The method is applicable for non-polar organic contaminants with an aqueou...view more

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    • Standard
      25 pages
      English language
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    • Standard
      25 pages
      English language

This document specifies one of the methods for evaluating the habitat function of soils and determining effects of soil contaminants and individual chemical substances on the reproduction of the oribatid mite Oppia nitens by dermal and alimentary uptake. This chronic (28-day) test is applicable to soils and soil materials of unknown quality (e.g., contaminated sites, amended soils, soils after remediation, agricultural or other sites under concern and waste materials). This method is not intende...view more

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    • Standard
      31 pages
      English language
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    • Standard
      34 pages
      French language

This document provides guidance on the use of chemical methods establishing the bioavailability of trace elements in soil and soil-like materials and to stimulate the use of bioavailability in assessments. The methods themselves are not subject of this document.

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    • Standard
      15 pages
      English language

This Standard specifies a method for sampling, handling and extracting enchytraeids from terrestrial field soils as a prerequisite for using these animals as bioindicators (e.g. to assess the quality of a soil as a habitat for organisms). Basic information on the ecology of enchytraeids and their use as bioindicators in the terrestrial environment is included in the Bibliography. This document applies to all terrestrial biotopes in which enchytraeids occur. The sampling design of field studies i...view more

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This document specifies a method for sampling, handling and extracting enchytraeids from terrestrial field soils as a prerequisite for using these animals as bioindicators (e.g. to assess the quality of a soil as a habitat for organisms).
Basic information on the ecology of enchytraeids and their use as bioindicators in the terrestrial environment is included in the Bibliography.
This document applies to all terrestrial biotopes in which enchytraeids occur. The sampling design of field studies...
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This document specifies a method for sampling, handling and extracting enchytraeids from terrestrial field soils as a prerequisite for using these animals as bioindicators (e.g. to assess the quality of a soil as a habitat for organisms). Basic information on the ecology of enchytraeids and their use as bioindicators in the terrestrial environment is included in the Bibliography. This document applies to all terrestrial biotopes in which enchytraeids occur. The sampling design of field studies i...view more

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    • Standard
      14 pages
      English language
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    • Standard
      15 pages
      French language

This document specifies a method for the measurement of several enzyme activities (arylsulfatase, α −glucosidase, β -glucosidase, Cellubisidase, β -Xylosidase, phosphodiesterase (PDE), chitinase, phosphomonoesterase (PME), leucine-aminopeptidase, Alanine-aminopeptidase) simultaneously (or not) using fluorigenic substrates in soil samples. Enzyme activities of soil vary seasonally and depend on the chemical, physical and biological characteristics of soil. Its application for the detection of har...view more

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    • Technical specification
      13 pages
      English language

This document specifies a method for determining the activity of dehydrogenases enzymes in soil using
2,3,5-triphenyltetrazolium chloride (TTC).

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    • Standard
      17 pages
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This document specifies a method for determining activity of dehydrogenases in soil, using
2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride (INT)[1]-[5]. As the INT reduction is
less sensitive to O2, the method is more robust than the TTC-method described in ISO 23753-1.

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    • Standard
      16 pages
      English language
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This document describes a method to compare the quality of soils by determining the fatty acid composition of the leaves of plant species grown in these soils. This method does not make it possible to determine an optimal value of the Omega-3 index and, therefore, cannot be used to determine the intrinsic quality of a soil from a specific area (regarded as homogeneous). The method can only be used to compare the quality of soils between various areas. This method is applicable to: — soils from c...view more

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    • Standard
      23 pages
      English language
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    • Standard
      25 pages
      French language

This document specifies a chronic test method for evaluating the habitat function of soils and determining effects of soil contaminants and substances on the reproduction of Hypoaspis aculeifer by ? mainly ? alimentary uptake. This method is applicable to soils and soil materials of unknown quality, e.g. from contaminated sites, amended soils, soils after remediation, industrial, agricultural or other sites under concern and waste materials (e.g. dredged material, municipal sludge from a wastewa...view more

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    • Standard
      21 pages
      English language
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    • Standard
      23 pages
      French language

This document specifies a method for determining the activity of dehydrogenases enzymes in soil using 2,3,5-triphenyltetrazolium chloride (TTC).

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    • Standard
      17 pages
      English language
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This document specifies a method for determining activity of dehydrogenases in soil, using 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride (INT)[1]-[5]. As the INT reduction is less sensitive to O2, the method is more robust than the TTC-method described in ISO 23753-1.

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    • Standard
      16 pages
      English language
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      1 day

This document specifies a protocol to identify ecotoxicological test specimens (mainly invertebrates and plants) to the species level, based on the DNA barcoding technique. This protocol can be used by laboratories performing DNA barcoding in order to standardize both the wet-lab and data analysis workflows as much as possible, and make them compliant with community standards and guidelines. This document does not intend to specify one particular strain for each test method, but to accurately do...view more

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    • Standard
      20 pages
      English language
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    • Standard
      21 pages
      French language

This document specifies a method for determining activity of dehydrogenases in soil, using 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride (INT)[1]-[5]. As the INT reduction is less sensitive to O2, the method is more robust than the TTC-method described in ISO 23753-1.

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    • Standard
      8 pages
      English language
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    • Standard
      8 pages
      French language

This document specifies a method for determining the activity of dehydrogenases enzymes in soil using 2,3,5-triphenyltetrazolium chloride (TTC).

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    • Standard
      9 pages
      English language
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    • Standard
      9 pages
      French language

This document specifies a method for sampling and handling earthworms from field soils as a
prerequisite for using these animals as bioindicators (e.g. to assess the quality of a soil as a habitat for
organisms).
This document applies to all terrestrial biotopes in which earthworms occur. The sampling design
of field studies in general is given in ISO 18400-101 and guidance on the determination of effects of
pollutants on earthworms in field situations is given in ISO 11268-3. These aspects...
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This document specifies a semi-static method for determining the effects of contaminants on growth
and survival of young snails, usually Helix aspersa aspersa Müller. The animals are exposed via the
cutaneous and digestive route using a test substrate (artificial or natural soil according to the objective
of the study) to which defined amounts of the following are added:
— substances, mixtures or preparations;
— soils (contaminated or of unknown quality) or waste materials.
This test takes...
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This document deals with the assessment of human exposure from ingestion of soil and soil materials. It specifies a physiologically based test procedure for the estimation of the human bioaccessibility of metals from contaminated soil in connection with the evaluation of the exposure related to human oral uptake. The method is a sequential extraction using synthetic gastrointestinal fluids and can be used to estimate oral bioaccessibility. Soils or other geological materials, in sieved form, are...view more

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    • Standard
      21 pages
      English language
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    • Standard
      22 pages
      French language

This document specifies a semi-static method for determining the effects of contaminants on growth and survival of young snails, usually Helix aspersa aspersa Müller. The animals are exposed via the cutaneous and digestive route using a test substrate (artificial or natural soil according to the objective of the study) to which defined amounts of the following are added:
- substances, mixtures or preparations;
- soils (contaminated or of unknown quality) or waste materials.
This test takes in...
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    • Standard
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This document specifies a method for the measurement of several hydrolase activities (arylamidase, arylsulfatase, β-galactosidase, α-glucosidase, β-glucosidase, N-acetyl-glucosaminidase, acid, alkaline and global phosphatases, urease) simultaneously (or not) in soil samples, using colorimetric substrates. Enzyme activities of soil vary seasonally and depend on soil chemical, physical and biological characteristics. This method can be applied either to detect harmful effects on soil enzyme activi...view more

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    • Standard
      29 pages
      English language
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    • Standard
      30 pages
      French language

This document specifies a method for sampling and handling earthworms from field soils as a prerequisite for using these animals as bioindicators (e.g. to assess the quality of a soil as a habitat for organisms).
This document applies to all terrestrial biotopes in which earthworms occur. The sampling design of field studies in general is given in ISO 18400‑101 and guidance on the determination of effects of pollutants on earthworms in field situations is given in ISO 11268‑3. These aspects can...
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This document specifies a laboratory test for characterizing the denitrifying enzyme activities in soils[5]. It globally characterizes the transformation of the nitrate form to the nitrous oxide and dinitrogen forms. This method was first proposed by Reference [5] with the acronym DEA for Denitrifying Enzyme Activities. It is a standardized technique used in numerous scientific studies. DEA estimates the process of denitrification of fresh soil samples incubated under optimal conditions of subst...view more

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    • Technical specification
      12 pages
      English language
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    • Technical specification
      12 pages
      French language

This document specifies a laboratory test for characterizing the ability (or inability) of soils to reduce the greenhouse gas N2O into N2 as it was previously shown that soils with a low ability to reduce N2O into N2 constitute situations with a risk of large emission of N2O[6], higher than those basically estimated by the use at the plot scale of the equations proposed in the IPCC guidelines for National Greenhouse Gas Inventories[10]. This test is performed in laboratory on a composite of siev...view more

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    • Technical specification
      13 pages
      English language
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    • Technical specification
      13 pages
      French language

This document specifies a semi-static method for determining the effects of contaminants on growth and survival of young snails, usually Helix aspersa aspersa Müller. The animals are exposed via the cutaneous and digestive route using a test substrate (artificial or natural soil according to the objective of the study) to which defined amounts of the following are added: - substances, mixtures or preparations; - soils (contaminated or of unknown quality) or waste materials. This test takes into ...view more

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    • Standard
      34 pages
      English language
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    • Standard
      36 pages
      French language

ISO 18187:2016 specifies a rapid method for assessing solid samples in an aerobic suspension, by determining the inhibition of dehydrogenase activity of Arthrobacter globiformis using the redox dye resazurin.
It is applicable for assessing the effect of water-soluble and solid matter bounded non-volatile contaminants of natural samples, such as soils and waste materials. The test yields a result within 6 h and can therefore be used for screening potentially contaminated material.

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      34 pages
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ISO 18311:2016 specifies a technique for determining the effects of anthropogenic impacts (e.g. substances) in the context of the prevailing environmental conditions on the feeding activity of soil organisms in the field. In addition, the use of this method for monitoring the biological quality of soil is described (see Annex A). The breakdown of organic matter by soil invertebrates and microorganisms is a crucial process that determines important soil functions such as nutrient availability for...view more

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      24 pages
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ISO 17601:2016 specifies the crucial steps of a quantitative real-time polymerase chain reaction (qPCR) method to measure the abundance of selected microbial gene sequences from soil DNA extract which provides an estimation of selected microbial groups.
It is noteworthy that the number of genes is not necessarily directly linked to the number of organisms that are measured. For example, the number of ribosomal operon is ranging from one copy to 20 copies in different bacterial phyla. Therefore,...
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    • Standard
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This document specifies a method for sampling and handling earthworms from field soils as a prerequisite for using these animals as bioindicators (e.g. to assess the quality of a soil as a habitat for organisms). This document applies to all terrestrial biotopes in which earthworms occur. The sampling design of field studies in general is given in ISO 18400‑101 and guidance on the determination of effects of pollutants on earthworms in field situations is given in ISO 11268‑3. These aspects can ...view more

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    • Standard
      19 pages
      English language
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    • Standard
      19 pages
      French language

ISO 17601:2016 specifies the crucial steps of a quantitative real-time polymerase chain reaction (qPCR) method to measure the abundance of selected microbial gene sequences from soil DNA extract which provides an estimation of selected microbial groups.
It is noteworthy that the number of genes is not necessarily directly linked to the number of organisms that are measured. For example, the number of ribosomal operon is ranging from one copy to 20 copies in different bacterial phyla. Therefore,...
view more

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    • Standard
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