prEN ISO 18862

Coffee and coffee products - Determination of acrylamide - Methods using HPLC-MS/MS and GC-MS after derivatization (ISO/DIS 18862:2024)

ISO 18862:2016 specifies methods for the determination of acrylamide in coffee and coffee products by extraction with water, clean-up by solid-phase extraction and determination by HPLC-MS/MS and GC-MS. It was validated in a method validation study on roasted coffee, soluble coffee, coffee substitutes and coffee products with ranges from 53 μg/kg to 612,1 μg/kg.

Kaffee und Kaffee-Erzeugnisse- Bestimmung von Acrylamid- Verfahren mittels HPLC-MS/MS und mittels GC-MS nach Derivatisierung (ISO/DIS 18862:2024)

Café et dérivés du café - Dosage de l'acrylamide - Méthodes par CLHP-SM/SM et CG-SM après dérivation (ISO/DIS 18862:2024)

Le présent document spécifie des méthodes de dosage de l'acrylamide dans le café et les dérivés du café par extraction à l'eau, purification par extraction en phase solide et dosage par CLHP-SM/SM et CG-SM. Il a été validé au cours d'une étude de validation de la méthode réalisée sur du café torréfié, du café soluble, des substituts de café et des dérivés du café dans des plages de concentration allant de 53 μg/kg à 612,1 μg/kg.

Kava in proizvodi iz kave - Določevanje akrilamida - Metode z uporabo HPLC-MS/MS in GC-MS po derivatizaciji (ISO/DIS 18862:2024)

General Information

• Status: Not Published
• Publication Date: 08-Mar-2026
• ICS: 67.140.20 - Coffee and coffee substitutes
• Technical Committee: CEN/TC 275 - Food analysis - Horizontal methods
• Drafting Committee: CEN/TC 275/WG 13 - Neoformed contaminants
• Current Stage: 4020 - Submission to enquiry - Enquiry
• Start Date: 15-Aug-2024
• Completion Date: 15-Aug-2024
• Ref Project: oSIST prEN ISO 18862:2024 - Coffee and coffee products - Determination of acrylamide - Methods using HPLC-MS/MS and GC-MS after derivatization (ISO/DIS 18862:2024)

Relations

• Revises: EN ISO 18862:2019 - Coffee and coffee products - Determination of acrylamide - Methods using HPLC-MS/MS and GC-MS after derivatization (ISO 18862:2016)
• Effective Date: 05-Jun-2024

Standards Content (Sample)

SLOVENSKI STANDARD
01-oktober-2024
Kava in proizvodi iz kave - Določevanje akrilamida - Metode z uporabo HPLC-
MS/MS in GC-MS po derivatizaciji (ISO/DIS 18862:2024)
Coffee and coffee products - Determination of acrylamide - Methods using HPLC-MS/MS
and GC-MS after derivatization (ISO/DIS 18862:2024)
Kaffee und Kaffee-Erzeugnisse- Bestimmung von Acrylamid- Verfahren mittels HPLC-
MS/MS und mittels GC-MS nach Derivatisierung (ISO/DIS 18862:2024)
Café et dérivés du café - Dosage de l'acrylamide - Méthodes par CLHP-SM/SM et CG-
SM après dérivation (ISO/DIS 18862:2024)
Ta slovenski standard je istoveten z: prEN ISO 18862
ICS:
67.140.20 Kava in kavni nadomestki Coffee and coffee substitutes
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

DRAFT
International
Standard
ISO/DIS 18862
ISO/TC 34/SC 15
Coffee and coffee products —
Secretariat: ICONTEC
Determination of acrylamide —
Voting begins on:
Methods using HPLC-MS/MS and GC-
2024-08-16
MS after derivatization
Voting terminates on:
2024-11-08
Café et dérivés du café — Dosage de l'acrylamide — Méthodes
par CLHP-SM/SM et CG-SM après dérivation
ICS: 67.140.20
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENTS AND APPROVAL. IT
IS THEREFORE SUBJECT TO CHANGE
AND MAY NOT BE REFERRED TO AS AN
INTERNATIONAL STANDARD UNTIL
PUBLISHED AS SUCH.
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Reference number
ISO/DIS 18862:2024(en)
DRAFT
ISO/DIS 18862:2024(en)
International
Standard
ISO/DIS 18862
ISO/TC 34/SC 15
Coffee and coffee products —
Secretariat: ICONTEC
Determination of acrylamide —
Voting begins on:
Methods using HPLC-MS/MS and GC-
MS after derivatization
Voting terminates on:
Café et dérivés du café — Dosage de l'acrylamide — Méthodes
par CLHP-SM/SM et CG-SM après dérivation
ICS: 67.140.20
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENTS AND APPROVAL. IT
IS THEREFORE SUBJECT TO CHANGE
AND MAY NOT BE REFERRED TO AS AN
INTERNATIONAL STANDARD UNTIL
PUBLISHED AS SUCH.
This document is circulated as received from the committee secretariat.
IN ADDITION TO THEIR EVALUATION AS
BEING ACCEPTABLE FOR INDUSTRIAL,
© ISO 2024
TECHNOLOGICAL, COMMERCIAL AND
USER PURPOSES, DRAFT INTERNATIONAL
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
STANDARDS MAY ON OCCASION HAVE TO
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Published in Switzerland Reference number
ISO/DIS 18862:2024(en)
ii
ISO/DIS 18862:2024(en)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 1
6 Apparatus . 3
7 Sampling . 4
8 Procedure . 4
8.1 General .4
8.2 Preparation of the sample extract .4
8.3 Clean-up of the extracts .5
8.3.1 Carrez precipitation .5
8.3.2 Solid phase extraction .5
8.4 HPLC-MS/MS measurement . .5
8.4.1 High-performance liquid chromatography (HPLC) .5
8.4.2 Identification and quantification by mass spectrometry (HPLC-MS/MS) .5
8.5 Measurement with GC-MS .6
8.5.1 Derivatization and sample preparation for gas chromatography .6
8.5.2 Gas chromatography .6
8.5.3 Identification and quantification by mass spectrometry .6
9 Calibration . 7
9.1 General advice .7
9.2 Determination of linearity and definition of the working range .7
9.3 Calibration with internal standard solution .7
9.4 Determination of the laboratory specific recovery .7
10 Evaluation . 7
10.1 Criteria for identification .7
10.2 Calculation and final results .8
11 Precision data . 8
11.1 General .8
11.2 Repeatability .8
11.3 Reproducibility .8
11.4 Recovery .9
12 Measurement uncertainty . 9
13 Test report . 9
Annex A (informative) Performance characteristics . 10
Annex B (informative) Examples of absorber materials .11
Annex C (informative) Examples of columns and analysis conditions.12
Annex D (Informative) Examples for sample preparation and chromatographic conditions
using LC-MS/MS .18
Bibliography .25

iii
ISO/DIS 18862:2024(en)
Foreword
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