CEN/TS 17709:2022

Plant biostimulants - Determination of Azotobacter spp.

This document was developed to provide the methodology for the enumeration and determination of Azotobacter sp. in plant biostimulant products in accordance with the Regulation (EU) 2019/1009 of the European Parliament and of the Council [1].

Pflanzen-Biostimulanzien - Bestimmung von Azotobacter spp.

Dieses Dokument wurde entwickelt, um die Methodik für die Zählung und Bestimmung von Azotobacter sp. in Pflanzen-Biostimulanzienprodukten in Übereinstimmung mit der Verordnung (EU) 2019/1009 des Europäischen Parlaments und des Rates [1] bereitzustellen.

Biostimulants des végétaux - Détermination d'Azotobacter spp.

Le présent document a été élaboré pour fournir la méthodologie de dénombrement et de détermination d’Azotobacter sp. dans les produits biostimulants des végétaux conformément au Règlement (UE) 2019/1009 du Parlement européen et du Conseil [1].

Rastlinski biostimulanti - Določanje Azotobacter spp.

Ta dokument zagotavlja metodologijo za štetje in določanje bakterij Azotobacter sp. v rastlinskih biostimulantih v skladu z Uredbo (EU) 2019/1009 Evropskega parlamenta in Sveta [1].

General Information

• Status: Published
• Publication Date: 22-Mar-2022
• ICS: 65.080 - Fertilizers
• Technical Committee: CEN/TC 455 - Plant Biostimulants and Agricultural Micro-Organisms
• Drafting Committee: CEN/TC 455/WG 3 - Pathogenic and non-pathogenic microorganisms
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 23-Mar-2022
• Due Date: 29-Aug-2022
• Completion Date: 23-Mar-2022
• Directive: 2019/1009 - Regulation (EU) 2019/1009 of the European Parliament and of the Council of 5 June 2019 laying down rules on the making available on the market of EU fertilising products and amending Regulations (EC) No 1069/2009 and (EC) No 1107/2009 and repealing Regulation (EC) No 2003/2003
• Mandate: M/564 - Fertilising products in support of Regulation (EU) 2019/1009
• Ref Project: SIST-TS CEN/TS 17709:2023 - Plant biostimulants - Determination of Azotobacter spp.

Relations

• Revised: FprEN 17709 - Plant biostimulants - Determination of Azobacter spp.
• Effective Date: 15-Feb-2023

Standards Content (Sample)

SLOVENSKI STANDARD
SIST-TS CEN/TS 17709:2023
01-februar-2023
Rastlinski biostimulanti - Določanje Azotobacter spp.
Plant biostimulants - Determination of Azotobacter spp.
Pflanzen-Biostimulanzien - Bestimmung von Azotobacter spp.
Biostimulants des végétaux - Détermination d'Azotobacter spp.
Ta slovenski standard je istoveten z: CEN/TS 17709:2022
ICS:
65.080 Gnojila Fertilizers
SIST-TS CEN/TS 17709:2023 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST-TS CEN/TS 17709:2023

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SIST-TS CEN/TS 17709:2023


CEN/TS 17709
TECHNICAL SPECIFICATION

SPÉCIFICATION TECHNIQUE

March 2022
TECHNISCHE SPEZIFIKATION
ICS 65.080
English Version

Plant biostimulants - Determination of Azotobacter spp.
Biostimulants des végétaux - Détermination Pflanzen-Biostimulanzien - Bestimmung von
d'Azotobacter spp. Azotobacter spp.
This Technical Specification (CEN/TS) was approved by CEN on 3 January 2022 for provisional application.

The period of validity of this CEN/TS is limited initially to three years. After two years the members of CEN will be requested to
submit their comments, particularly on the question whether the CEN/TS can be converted into a European Standard.

CEN members are required to announce the existence of this CEN/TS in the same way as for an EN and to make the CEN/TS
available promptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in
parallel to the CEN/TS) until the final decision about the possible conversion of the CEN/TS into an EN is reached.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATIO N

EUROPÄISCHES KOMITEE FÜR NO RMU N G

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2022 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN/TS 17709:2022 E
worldwide for CEN national Members.

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SIST-TS CEN/TS 17709:2023
CEN/TS 17709:2022 (E)
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 6
2 Normative references . 6
3 Terms and definitions . 6
4 Enumeration of Azotobacter spp . 6
4.1 General . 6
4.2 Sample preparation . 6
4.3 Serial dilution . 7
4.4 Plate counts of Azotobacter sp in sterile diluent . 8
4.5 Spread-plate counting with ASHBY SUCROSE AGAR [6] . 8
4.6 Calculation . 8
5 Species determination of Azotobacter sp. via genetic analysis . 8
5.1 General . 8
5.2 Preparation of the sample for the genomic DNA extraction . 8
Annex A (informative) Formula of culture media . 11
Bibliography .
...