Organic and organo-mineral fertilizers - Determination of the biuret content by high-performance liquid chromatography (HPLC)

This document is applicable to fertilizing products, which are classified as PFC 1(A) and PFC 1(B) or the PFC 1(A) and PFC 1(B) component in PFC 7 of Regulation (EU) 2019/1009 [4]. However, the present method was not validated for blends.
This document specifies a method for the determination of the biuret content by high-performance liquid chromatography (HPLC) with UV detector. The method is applicable to organic and organo-mineral fertilizers containing urea.
Nowadays, there is a method standardized as EN 15479 that allows the determination of biuret in urea by spectrophotometric detection. Organic and organo-mineral fertilizers contain organic matter and other compounds apart from urea that would interfere in a spectrophotometric method. HPLC allows an accurate determination of biuret by separating it from possible interfering compounds [2] [3].

Organische und organisch-mineralische Düngemittel - Bestimmung des Biuretgehalts mittels Hochleistungs-Flüssigkeitschromatographie (HPLC)

Dieses Dokument ist für Düngeprodukte anzuwenden, die den Kategorien PFC 1(A) und PFC 1(B) zugeordnet oder als Komponente der Kategorien PFC 1(A) und PFC 1(B) in PFC 7 der Verordnung (EU) 2019/1009 [4] eingestuft sind. Das vorliegende Verfahren wurde jedoch nicht für Mischungen validiert.
Dieses Dokument legt ein Verfahren zur Bestimmung des Biuretgehalts mittels Hochleistungs-Flüssigkeitschromatographie (HPLC) mit UV Detektor fest. Das Verfahren ist für organische und organisch-mineralische Düngemittel, die Harnstoff enthalten, anzuwenden.
Heutzutage gibt es ein als EN 15479 genormtes Verfahren, das die Bestimmung von Biuret in Harnstoff durch spektrophotometrische Detektion ermöglicht. Organische und organisch-mineralische Düngemittel enthalten neben Harnstoff auch andere Verbindungen, die bei einem spektralphotometrischen Verfahren stören würden. Die HPLC ermöglicht eine genaue Bestimmung von Biuret, indem sie es von möglichen störenden Verbindungen trennt [2] [3].

Engrais organiques et organo-minéraux - Détermination de la teneur en biuret par chromatographie liquide à haute performance (HPLC)

Le présent document est applicable aux fertilisants qui sont classés dans les catégories PFC 1(A) et PFC 1(B) ou comme composants de PFC 1(A) et de PFC 1(B) dans la catégorie PFC 7 du Règlement (UE) 2019/1009 [4]. Toutefois, la présente méthode n’a pas été validée pour les mélanges.
Le présent document spécifie une méthode pour la détermination de la teneur en biuret par chromatographie en phase liquide haute performance (HPLC) avec un détecteur UV. La méthode est applicable aux engrais organiques et organo-minéraux contenant de l’urée.
De nos jours, il existe une méthode normalisée, l’EN 15479, qui permet de doser le biuret dans l’urée par détection spectrophotométrique. Cependant, les engrais organiques et organo-minéraux contiennent de la matière organique et des composés autres que l’urée qui interfèreraient en cas d’utilisation d’une méthode spectrophotométrique. La HPLC permet un dosage exact du biuret en le séparant des composés pouvant interférer [2] [3].

Organska in organsko-mineralna gnojila - Določevanje biureta s tekočinsko kromatografijo visoke ločljivosti (HPLC)

Ta dokument vzpostavlja metodologijo za določevanje vsebnosti biureta.

General Information

Status
Published
Publication Date
05-Apr-2022
Current Stage
6060 - Definitive text made available (DAV) - Publishing
Start Date
06-Apr-2022
Due Date
04-Feb-2023
Completion Date
06-Apr-2022

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Standards Content (Sample)

SLOVENSKI STANDARD
SIST-TS CEN/TS 17765:2023
01-februar-2023
Organska in organsko-mineralna gnojila - Določevanje biureta s tekočinsko
kromatografijo visoke ločljivosti (HPLC)
Organic and organo-mineral fertilizers - Determination of the biuret content by high-
performance liquid chromatography (HPLC)
Organische und organisch-mineralische Düngemittel - Bestimmung des Biuretgehalts
mittels Hochleistungs-Flüssigkeitschromatographie (HPLC)
Engrais organiques et organo-minéraux - Détermination de la teneur en biuret par
chromatographie liquide à haute performance (HPLC)
Ta slovenski standard je istoveten z: CEN/TS 17765:2022
ICS:
65.080 Gnojila Fertilizers
SIST-TS CEN/TS 17765:2023 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST-TS CEN/TS 17765:2023

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SIST-TS CEN/TS 17765:2023


CEN/TS 17765
TECHNICAL SPECIFICATION

SPÉCIFICATION TECHNIQUE

April 2022
TECHNISCHE SPEZIFIKATION
ICS 65.080
English Version

Organic and organo-mineral fertilizers - Determination of
the biuret content by high-performance liquid
chromatography (HPLC)
Engrais organiques et organo-minéraux - Organische und organisch-mineralische Düngemittel -
Détermination de la teneur en biuret Bestimmung des Biuretgehalts
This Technical Specification (CEN/TS) was approved by CEN on 13 March 2022 for provisional application.

The period of validity of this CEN/TS is limited initially to three years. After two years the members of CEN will be requested to
submit their comments, particularly on the question whether the CEN/TS can be converted into a European Standard.

CEN members are required to announce the existence of this CEN/TS in the same way as for an EN and to make the CEN/TS
available promptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in
parallel to the CEN/TS) until the final decision about the possible conversion of the CEN/TS into an EN is reached.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2022 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN/TS 17765:2022 E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------
SIST-TS CEN/TS 17765:2023
CEN/TS 17765:2022 (E)
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 5
5 Reagents . 5
6 Apparatus . 6
7 Procedure . 6
7.1 Sampling . 6
7.2 Calibration solutions . 7
7.3 Preparation of the sample . 7
7.4 Extraction of the sample and preparation of the test solution.
...

SLOVENSKI STANDARD
kSIST-TS FprCEN/TS 17765:2021
01-december-2021
Organska in organsko-mineralna gnojila - Določevanje biureta
Organic and organo-mineral fertilizers - Determination of the biuret content
Organische und organisch-mineralische Düngemittel - Bestimmung des Biuretgehalts
Ta slovenski standard je istoveten z: FprCEN/TS 17765
ICS:
65.080 Gnojila Fertilizers
kSIST-TS FprCEN/TS 17765:2021 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
kSIST-TS FprCEN/TS 17765:2021

---------------------- Page: 2 ----------------------
kSIST-TS FprCEN/TS 17765:2021


FINAL DRAFT
TECHNICAL SPECIFICATION
FprCEN/TS 17765
SPÉCIFICATION TECHNIQUE

TECHNISCHE SPEZIFIKATION

November 2021
ICS 65.080
English Version

Organic and organo-mineral fertilizers - Determination of
the biuret content
 Organische und organisch-mineralische Düngemittel -
Bestimmung des Biuretgehalts


This draft Technical Specification is submitted to CEN members for Vote. It has been drawn up by the Technical Committee
CEN/TC 260.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.

Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.

Warning : This document is not a Technical Specification. It is distributed for review and comments. It is subject to change
without notice and shall not be referred to as a Technical Specification.


EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2021 CEN All rights of exploitation in any form and by any means reserved Ref. No. FprCEN/TS 17765:2021 E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------
kSIST-TS FprCEN/TS 17765:2021
FprCEN/TS 17765:2021 (E)
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 5
5 Reagents . 5
6 Apparatus . 6
7 Procedure . 6
7.1 Sampling . 6
7.2 Calibration solutions . 7
7.3 Preparation of the sample . 7
7.4 Extraction of the sample and preparation of the test solution. 7
7.5 HPLC conditions . 8
7.6 Preparation of the standard curve . 8
7.7 Determination of biuret in the test solutions .
...

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