EN 17711:2024
(Main)Plant biostimulants - Detection of Vibrio spp.
Plant biostimulants - Detection of Vibrio spp.
This document specifies a horizontal method for the detection of enteropathogenic Vibrio species (spp.), which causes human illness in or via the intestinal tract. The species detectable by the methods specified include Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus.
It is applicable to the microbial plant biostimulants.
NOTE 1 The World Health Organization (WHO) has identified that V. parahaemolyticus, V. cholerae and V. vulnificus are the major contaminants of Vibrio spp. [2].
NOTE 2 For confirmation, it is possible to use PCR (Polymerase Chain Reaction) tests; in this case a validation is carried out by the laboratory for the procedure and data generated.
This document is applicable to the blends of fertilizing products where a blend is a mix of at least two of the following component EU fertilising products categories: Fertilizers, Liming Materials, Soil Improvers, Growing Media, Plant Biostimulants, and where the following category Plant Biostimulants is the highest % in the blend by mass or volume, or in the case of liquid form by dry mass. If Plant Biostimulants is not the highest % in the blend, the European Standard for the highest % of the blend applies. In case a blend of fertilizing products is composed of components in equal quantity or in case the component EU fertilising products used for the blend have identical formulations , the user decides which standard to apply.
Pflanzen-Biostimulanzien - Nachweis von Vibrio spp.
Dieses Dokument legt ein horizontales Verfahren zum Nachweis von enteropathogenen Vibrio spp. fest, die Erkrankungen des Menschen am oder über den Darmtrakt verursachen [1]. Die Spezies, deren Nachweis die hier aufgeführten Verfahren einbeziehen, sind Vibrio parahaemolyticus, Vibrio cholerae und Vibrio vulnificus.
Das Dokument gilt für
- mikrobielle Pflanzen-Biostimulanzien.
ANMERKUNG 1 Die Weltgesundheitsorganisation (WHO; en: World Health Organization) hat V. parahaemolyticus, V. cholerae und V. vulnificus als die wichtigsten Kontaminanten von Vibrio spp. identifiziert [1].
ANMERKUNG 2 Eine Bestätigung durch PCR Tests ist möglich; in diesem Fall muss das Labor das Verfahren und die erzeugten Daten validieren.
Biostimulants des végétaux - Détection de Vibrio spp.
Le présent document spécifie une méthode horizontale pour la recherche des espèces entéropathogènes de Vibrio (spp.) provoquant des maladies dans ou via le tractus intestinal chez l’homme. Les espèces détectables par les méthodes spécifiées incluent Vibrio parahaemolyticus, Vibrio cholerae et Vibrio vulnificus.
Il est applicable aux biostimulants microbiens des végétaux.
NOTE 1 L’Organisation Mondiale de la Santé (OMS) a identifié V. parahaemolyticus, V. cholerae et V. vulnificus comme les principales espèces de Vibrio contaminantes [2].
NOTE 2 Pour la confirmation, il est possible de recourir aux essais d’amplification en chaîne par polymérase (PCR) ; dans ce cas, une validation est réalisée par le laboratoire pour le mode opératoire et les données générées.
Le présent document est applicable aux mélanges de fertilisants constitués d’au moins deux composants appartenant aux catégories de fertilisants UE suivantes : engrais, amendements minéraux basiques, amendements du sol, supports de culture, biostimulants des végétaux et, dans lesquels la catégorie Biostimulants des végétaux représente le % le plus élevé du mélange en masse ou en volume, ou dans le cas d’un liquide, en masse sèche. Si les biostimulants des végétaux ne représentent pas le % le plus élevé du mélange, la Norme européenne relative au composant représentant le % le plus élevé du mélange s’applique. Dans le cas où un mélange de fertilisants contient des composants en quantité égale ou dans le cas où les composants des fertilisants UE utilisés pour le mélange ont des formulations identiques, l’utilisateur décide quelle norme appliquer.
Rastlinski biostimulanti - Ugotavljanje prisotnosti Vibrio spp.
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
oSIST prEN 17711:2023
01-maj-2023
Rastlinski biostimulanti - Ugotavljanje prisotnosti Vibrio spp.
Plant biostimulants - Detection of Vibrio spp.
Pflanzen-Biostimulanzien - Nachweis von Vibrio spp.
Biostimulants des végétaux - Détection de Vibrio spp.
Ta slovenski standard je istoveten z: prEN 17711
ICS:
65.080 Gnojila Fertilizers
oSIST prEN 17711:2023 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
oSIST prEN 17711:2023
oSIST prEN 17711:2023
DRAFT
EUROPEAN STANDARD
prEN 17711
NORME EUROPÉENNE
EUROPÄISCHE NORM
April 2023
ICS 65.080 Will supersede CEN/TS 17711:2022
English Version
Plant biostimulants - Detection of Vibrio spp.
Biostimulants des végétaux - Détection de Vibrio spp. Pflanzen-Biostimulanzien - Nachweis von Vibrio spp.
This draft European Standard is submitted to CEN members for enquiry. It has been drawn up by the Technical Committee
CEN/TC 455.
If this draft becomes a European Standard, CEN members are bound to comply with the CEN/CENELEC Internal Regulations
which stipulate the conditions for giving this European Standard the status of a national standard without any alteration.
This draft European Standard was established by CEN in three official versions (English, French, German). A version in any other
language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC
Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.
Warning : This document is not a European Standard. It is distributed for review and comments. It is subject to change without
notice and shall not be referred to as a European Standard.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATIO N
EUROPÄISCHES KOMITEE FÜR NORMUN G
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2023 CEN All rights of exploitation in any form and by any means reserved Ref. No. prEN 17711:2023 E
worldwide for CEN national Members.
oSIST prEN 17711:2023
prEN 17711:2023 (E)
Contents Page
European foreword . 6
Introduction . 7
1 Scope . 8
2 Normative references . 8
3 Terms and definitions . 8
4 Principle . 9
4.1 General . 9
4.2 Primary enrichment in a liquid selective medium . 9
4.3 Secondary enrichment in a liquid selective medium . 9
4.4 Isolation and identification . 10
4.5 Confirmation . 10
5 Culture media and reagents . 10
5.1 Enrichment medium: alkaline saline peptone water (ASPW) . 10
5.2 Solid selective isolation media . 10
5.2.1 First medium: thiosulphate, citrate, bile and sucrose agar medium (TCBS) . 10
5.2.2 Second medium . 11
5.3 Saline nutrient agar (SNA) . 11
5.4 Reagent for detection of oxidase . 11
5.5 Reagent for Biochemical tests . 11
5.5.1 L-lysine decarboxylase saline medium (LDC) . 11
5.5.2 Arginine dihydrolase saline medium (ADH) . 11
5.5.3 Reagent for detection of β-galactosidase . 11
5.5.4 Saline medium for detection of indole . 11
5.5.5 Saline peptone water . 12
5.5.6 Sodium chloride solution . 12
5.5.7 Toluene, p.a. . 12
6 Equipment and consumables . 12
7 Sampling . 12
8 Preparation of the test sample . 12
9 Procedure (see Figure A.1) . 13
9.1 Test portion and initial suspension . 13
9.2 Primary selective enrichment . 13
9.3 Secondary selective enrichment . 14
9.4 Isolation and identification . 14
9.5 Confirmation . 15
9.5.1 General . 15
9.5.2 Selection of colonies for confirmation and preparation of pure cultures . 15
9.5.3 Tests for presumptive identification . 16
9.5.4 Biochemical confirmation . 16
10 Expression of results . 18
11 Performance characteristics of the method . 18
11.1 Sensitivity . 18
oSIST prEN 17711:2023
prEN 17711:2023 (E)
11.2 Specificity . 18
11.3 LOD50 . 18
11.4 Precision . 18
12 Test report . 18
Annex A (normative) Diagram of procedure . 20
Annex B (normative) Composition and preparation of the culture media and reagents . 22
B.1 Introduction . 22
B.2 Water . 22
B.3 Alkaline saline peptone water (ASPW) . 22
B.3.1 Composition . 22
B.3.2 Preparation . 22
B.4 Thiosulfate citrate bile and sucrose agar (TCBS) . 23
B.4.1 Composition . 23
B.4.2 Preparation . 23
B.4.3 Preparation of the agar dishes . 23
B.5 Saline nutrient agar (SNA) . 23
B.5.1 Composition . 23
B.5.2 Preparation . 24
B.5.3 Preparation of the agar dishes . 24
B.5.4 Preparation of slants of saline nutrient agar . 24
B.6 Reagent for detection of oxidase . 24
B.6.1 Composition . 24
B.6.2 Preparation . 24
B.7 L-lysine decarboxylase saline medium (LDC). 24
B.7.1 Composition . 24
B.7.2 Preparation . 24
B.8 Arginine dihydrolase saline medium (ADH) . 25
B.8.1 Composition . 25
B.8.2 Preparation . 25
B.9 Detection of β-galactosidase . 25
B.9.1 ONPG solution . 25
B.9.2 Buffer solution . 25
B.9.3 Complete reagent . 26
B.10 Saline medium for detection of indole . 26
B.10.1 Tryptophan saline medium . 26
B.10.2 Kovacs reagent . 26
B.11 Saline peptone water . 27
B.11.1 Composition . 27
oSIST prEN 17711:2023
prEN 17711:2023 (E)
B.11.2 Preparation . 27
B.12 Sodium chloride solution . 27
B.12.1 Composition .
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