EN 17711:2024
(Main)Plant biostimulants - Detection of Vibrio spp.
Plant biostimulants - Detection of Vibrio spp.
This document specifies a horizontal method for the detection of enteropathogenic Vibrio spp., which causes human illness in or via the intestinal tract [1]. The species detectable by the methods specified include Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus.
It is applicable to the following:
- microbial plant biostimulants.
NOTE 1 The World Health Organization (WHO) has identified that V. parahaemolyticus, V. cholerae and V. vulnificus are the major contaminants of Vibrio spp. [1].
NOTE 2 For confirmation, it is possible to use PCR tests; in this case the laboratory must validate the procedure and data generated.
Pflanzen-Biostimulanzien - Nachweis von Vibrio spp.
Dieses Dokument legt ein horizontales Verfahren zum Nachweis von enteropathogenen Vibrio spp. fest, die Erkrankungen des Menschen am oder über den Darmtrakt verursachen [1]. Die Spezies, deren Nachweis die hier aufgeführten Verfahren einbeziehen, sind Vibrio parahaemolyticus, Vibrio cholerae und Vibrio vulnificus.
Das Dokument gilt für
- mikrobielle Pflanzen-Biostimulanzien.
ANMERKUNG 1 Die Weltgesundheitsorganisation (WHO; en: World Health Organization) hat V. parahaemolyticus, V. cholerae und V. vulnificus als die wichtigsten Kontaminanten von Vibrio spp. identifiziert [1].
ANMERKUNG 2 Eine Bestätigung durch PCR Tests ist möglich; in diesem Fall muss das Labor das Verfahren und die erzeugten Daten validieren.
Biostimulants des végétaux - Détection de Vibrio spp.
Le présent document spécifie une méthode horizontale pour la recherche des espèces entéropathogènes de Vibrio provoquant des maladies dans ou via le tractus intestinal chez l’homme [1]. Les espèces détectables par les méthodes spécifiées incluent Vibrio parahaemolyticus, Vibrio cholerae et Vibrio vulnificus.
Il s’applique :
- aux biostimulants microbiens des végétaux.
NOTE 1 L’Organisation Mondiale de la Santé (OMS) a identifié V. parahaemolyticus, V. cholerae et V. vulnificus comme les principales espèces de Vibrio contaminantes [1].
NOTE 2 Pour la confirmation, il est possible de recourir aux essais de PCR ; dans ce cas, le laboratoire doit valider le mode opératoire et les données générées.
Rastlinski biostimulanti - Ugotavljanje prisotnosti Vibrio spp.
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
oSIST prEN 17711:2023
01-maj-2023
Rastlinski biostimulanti - Ugotavljanje prisotnosti Vibrio spp.
Plant biostimulants - Detection of Vibrio spp.
Pflanzen-Biostimulanzien - Nachweis von Vibrio spp.
Biostimulants des végétaux - Détection de Vibrio spp.
Ta slovenski standard je istoveten z: prEN 17711
ICS:
65.080 Gnojila Fertilizers
oSIST prEN 17711:2023 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
oSIST prEN 17711:2023
oSIST prEN 17711:2023
DRAFT
EUROPEAN STANDARD
prEN 17711
NORME EUROPÉENNE
EUROPÄISCHE NORM
April 2023
ICS 65.080 Will supersede CEN/TS 17711:2022
English Version
Plant biostimulants - Detection of Vibrio spp.
Biostimulants des végétaux - Détection de Vibrio spp. Pflanzen-Biostimulanzien - Nachweis von Vibrio spp.
This draft European Standard is submitted to CEN members for enquiry. It has been drawn up by the Technical Committee
CEN/TC 455.
If this draft becomes a European Standard, CEN members are bound to comply with the CEN/CENELEC Internal Regulations
which stipulate the conditions for giving this European Standard the status of a national standard without any alteration.
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language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC
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aware and to provide supporting documentation.
Warning : This document is not a European Standard. It is distributed for review and comments. It is subject to change without
notice and shall not be referred to as a European Standard.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATIO N
EUROPÄISCHES KOMITEE FÜR NORMUN G
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2023 CEN All rights of exploitation in any form and by any means reserved Ref. No. prEN 17711:2023 E
worldwide for CEN national Members.
oSIST prEN 17711:2023
prEN 17711:2023 (E)
Contents Page
European foreword . 6
Introduction . 7
1 Scope . 8
2 Normative references . 8
3 Terms and definitions . 8
4 Principle . 9
4.1 General . 9
4.2 Primary enrichment in a liquid selective medium . 9
4.3 Secondary enrichment in a liquid selective medium . 9
4.4 Isolation and identification . 10
4.5 Confirmation . 10
5 Culture media and reagents . 10
5.1 Enrichment medium: alkaline saline peptone water (ASPW) . 10
5.2 Solid selective isolation media . 10
5.2.1 First medium: thiosulphate, citrate, bile and sucrose agar medium (TCBS) . 10
5.2.2 Second medium . 11
5.3 Saline nutrient agar (SNA) . 11
5.4 Reagent for detection of oxidase . 11
5.5 Reagent for Biochemical tests . 11
5.5.1 L-lysine decarboxylase saline medium (LDC) . 11
5.5.2 Arginine dihydrolase saline medium (ADH) . 11
5.5.3 Reagent for detection of β-galactosidase . 11
5.5.4 Saline medium for detection of indole . 11
5.5.5 Saline peptone water . 12
5.5.6 Sodium chloride solution . 12
5.5.7 Toluene, p.a. . 12
6 Equipment and consumables . 12
7 Sampling . 12
8 Preparation of the test sample . 12
9 Procedure (see Figure A.1) . 13
9.1 Test portion and initial suspension . 13
9.2 Primary selective enrichment . 13
9.3 Secondary selective enrichment . 14
9.4 Isolation and identification . 14
9.5 Confirmation . 15
9.5.1 General . 15
9.5.2 Selection of colonies for confirmation and preparation of pure cultures . 15
9.5.3 Tests for presumptive identification . 16
9.5.4 Biochemical confirmation . 16
10 Expression of results . 18
11 Performance characteristics of the method . 18
11.1 Sensitivity . 18
oSIST prEN 17711:2023
prEN 17711:2023 (E)
11.2 Specificity . 18
11.3 LOD50 . 18
11.4 Precision . 18
12 Test report . 18
Annex A (normative) Diagram of procedure . 20
Annex B (normative) Composition and preparation of the culture media and reagents . 22
B.1 Introduction . 22
B.2 Water . 22
B.3 Alkaline saline peptone water (ASPW) . 22
B.3.1 Composition . 22
B.3.2 Preparation . 22
B.4 Thiosulfate citrate bile and sucrose agar (TCBS) . 23
B.4.1 Composition . 23
B.4.2 Preparation . 23
B.4.3 Preparation of the agar dishes . 23
B.5 Saline nutrient agar (SNA) . 23
B.5.1 Composition . 23
B.5.2 Preparation . 24
B.5.3 Preparation of the agar dishes . 24
B.5.4 Preparation of slants of saline nutrient agar . 24
B.6 Reagent for detection of oxidase . 24
B.6.1 Composition . 24
B.6.2 Preparation . 24
B.7 L-lysine decarboxylase saline medium (LDC). 24
B.7.1 Composition . 24
B.7.2 Preparation . 24
B.8 Arginine dihydrolase saline medium (ADH) . 25
B.8.1 Composition . 25
B.8.2 Preparation . 25
B.9 Detection of β-galactosidase . 25
B.9.1 ONPG solution . 25
B.9.2 Buffer solution . 25
B.9.3 Complete reagent . 26
B.10 Saline medium for detection of indole . 26
B.10.1 Tryptophan saline medium . 26
B.10.2 Kovacs reagent . 26
B.11 Saline peptone water . 27
B.11.1 Composition . 27
oSIST prEN 17711:2023
prEN 17711:2023 (E)
B.11.2 Preparation . 27
B.12 Sodium chloride solution . 27
B.12.1 Composition .
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