EN 13368-1:2014
(Main)Fertilizers - Determination of chelating agents in fertilizers by chromatography - Part 1: Determination of EDTA, HEEDTA and DTPA by ion chromatography
Fertilizers - Determination of chelating agents in fertilizers by chromatography - Part 1: Determination of EDTA, HEEDTA and DTPA by ion chromatography
This European Standard specifies a method for the chromatographic determination of the total amount of each of the individual chelating agents EDTA, HEEDTA, and DTPA in fertilizers containing one or more of these substances. The method allows the identification and the determination of the total water soluble fraction of each of these chelating agents. It does not allow to distinguish between the free form and the metal bound form of the chelating agents.
NOTE EDTA, HEEDTA and DTPA are abbreviations used in this European Standard for the sake of simplicity. For complete names see Annex A.
This method applies to fertilizers containing chelates of one or more of the following micro-nutrients: cobalt, copper, iron, manganese, zinc and with a mass fraction of at least 0,1 %.
Düngemittel - Bestimmung von Chelatbildnern in Düngemitteln mit Chromatographie - Teil 1: Bestimmung von EDTA, HEEDTA und DTPA mit Ionenchromatographie
Diese Europäische Norm legt ein Verfahren zur chromatographischen Bestimmung des Gesamtgehalts von jedem einzelnen der Chelatbildner EDTA, HEEDTA und DTPA in Düngemitteln fest, die eine oder mehrere dieser Substanzen enthalten. Das Verfahren ermöglicht die Identifizierung und Bestimmung des Gesamtanteils an wasserlöslichen Bestandteilen jedes dieser Chelatbildner. Es erlaubt keine Unterscheidung zwischen der freien Form und der metallisch gebundenen Form der Chelatbildner.
ANMERKUNG EDTA, HEEDTA und DTPA sind Abkürzungen, die in dieser Europäischen Norm der Einfachheit halber verwendet werden. Bezüglich der vollständigen Bezeichnungen siehe Anhang A.
Dieses Verfahren gilt für Düngemittel, die Chelate von einem oder mehreren der folgenden Spurennährstoffe enthalten: Kobalt, Kupfer, Eisen, Mangan, Zink mit einem Massenanteil von mindestens 0,1 %.
Engrais - Détermination des agents chélatants dans les engrais par chromatographie - Partie 1: Détermination du EDTA, HEEDTA et DTPA par chromatographie ionique
La présente norme européenne spécifie une méthode pour la détermination par chromatographie de la quantité totale de chacun des agents chélatants individuels EDTA, HEEDTA et DTPA dans les engrais contenant une ou plusieurs de ces substances. La méthode permet d’identifier et de déterminer la proportion totale soluble dans l’eau de chacun de ces agents chélatants. Elle ne permet pas de faire la distinction entre la forme libre et la forme métallique de l’agent chélatant.
NOTE EDTA, HEEDTA et DTPA sont des abréviations employées dans la présente norme européenne dans un souci de simplicité : pour les noms complets, se reporter à l’annexe A.
La présente méthode s’applique aux engrais contenant des chélates d’au moins un des oligo-éléments suivants : cobalt, cuivre, fer, manganèse, zinc, avec une fraction massique d’au moins 0,1 %.
Gnojila - Določevanje sredstev za kelatiziranje v gnojilih s kromatografijo - 1. del: Določevanje EDTA, HEEDTA in DTPA z ionsko kromatografijo
General Information
- Status
- Published
- Publication Date
- 21-Jan-2014
- Technical Committee
- CEN/TC 260 - Fertilizers and liming materials
- Drafting Committee
- CEN/TC 260/WG 5 - Determination of chelating agents
- Current Stage
- 9093 - Decision to confirm - Review Enquiry
- Start Date
- 05-Jun-2025
- Completion Date
- 23-Sep-2025
Relations
- Effective Date
- 07-May-2011
Overview
EN 13368-1:2014 is a European standard established by CEN that defines a precise chromatographic method for determining the content of specific chelating agents-EDTA, HEEDTA, and DTPA-in fertilizers. These chelating agents play a crucial role in nutrient delivery by stabilizing trace elements such as cobalt, copper, iron, manganese, and zinc. The standard focuses on the total water-soluble fraction of these chelating agents in fertilizers, using ion chromatography to ensure accurate identification and quantification.
This standard is essential for laboratories, manufacturers, and quality control entities within the fertilizer industry to ensure accurate determination of chelating agents, thereby guaranteeing product consistency and compliance with regulatory requirements.
Key Topics
Scope and Purpose
EN 13368-1:2014 applies specifically to fertilizers containing at least 0.1% (mass fraction) of chelates of micro-nutrients complexed with EDTA, HEEDTA, or DTPA. The standard targets the combined measurement of free and metal-bound forms but does not differentiate between them.Analytical Principle
The method works by substituting micro-nutrients in the aqueous fertilizer extract with iron(III) ions. The resulting iron chelates undergo separation through ion exchange chromatography using an anion exchange column and nitrate-acetate eluent at controlled pH. Detection relies on UV photometry at 330 nm, enhanced by a post-column reaction with diluted perchloric acid.Interferences and Limitations
Various salts and compounds may interfere with the chromatographic process, including high concentrations of chloride and certain polyamino polycarboxylic acids. The method mitigates interference through the choice of column and specific reagent treatment protocols.Reagents and Equipment
Utilizes analytical grade reagents such as nitric acid, sodium hydroxide, and specialized stock solutions of EDTA, HEEDTA, and DTPA. Essential apparatus includes a liquid chromatograph equipped with an isocratic pump, injection valve, anion exchange column, UV/VIS detector, and membrane filters with 0.2 μm and 0.45 μm porosities.Procedure Overview
The standardized procedure involves sample extraction with water, replacement of native micro-nutrients by iron(III), filtration, and chromatographic analysis using calibration standards. Results are expressed as mass fractions of chelating agents calculated against calibration graphs.
Applications
Quality Control in Fertilizer Production
Manufacturers use EN 13368-1:2014 to quantify critical chelating agents, essential for optimizing the bioavailability of micronutrients and ensuring product performance.Regulatory Compliance
The standardized method supports compliance with European fertilizer legislation by providing a reliable, reproducible test for ingredient verification.Research and Development
Fertilizer R&D labs apply this method to develop and optimize chelate formulations, improving nutrient efficiency and environmental safety.Agricultural Testing
Enables testing laboratories to verify fertilizer content, thereby ensuring accurate nutrient delivery recommendations for agricultural use.
Related Standards
EN 1482-1: Sampling
Provides guidance on fertilizer sampling techniques which complement the sample preparation requirements in EN 13368-1:2014.EN 1482-2: Sample Preparation
Details procedures for preparing fertilizer samples prior to chromatographic analysis for accurate and consistent results.EN 12944 Series: Vocabulary
Defines key terminology related to fertilizers and liming materials, supporting precise communication and understanding within the industry.EN ISO 3696: Water Quality
Specifies the standards for laboratory water quality, which is critical for ensuring reproducibility and accuracy in chromatographic analysis.
Keywords
Fertilizers, chelating agents, EDTA determination, HEEDTA, DTPA, ion chromatography, nutrient analysis, micro-nutrients, quality control, fertilizer standards, CEN standard, fertilizer testing, chromatographic method, trace elements, agricultural fertilizers.
Frequently Asked Questions
EN 13368-1:2014 is a standard published by the European Committee for Standardization (CEN). Its full title is "Fertilizers - Determination of chelating agents in fertilizers by chromatography - Part 1: Determination of EDTA, HEEDTA and DTPA by ion chromatography". This standard covers: This European Standard specifies a method for the chromatographic determination of the total amount of each of the individual chelating agents EDTA, HEEDTA, and DTPA in fertilizers containing one or more of these substances. The method allows the identification and the determination of the total water soluble fraction of each of these chelating agents. It does not allow to distinguish between the free form and the metal bound form of the chelating agents. NOTE EDTA, HEEDTA and DTPA are abbreviations used in this European Standard for the sake of simplicity. For complete names see Annex A. This method applies to fertilizers containing chelates of one or more of the following micro-nutrients: cobalt, copper, iron, manganese, zinc and with a mass fraction of at least 0,1 %.
This European Standard specifies a method for the chromatographic determination of the total amount of each of the individual chelating agents EDTA, HEEDTA, and DTPA in fertilizers containing one or more of these substances. The method allows the identification and the determination of the total water soluble fraction of each of these chelating agents. It does not allow to distinguish between the free form and the metal bound form of the chelating agents. NOTE EDTA, HEEDTA and DTPA are abbreviations used in this European Standard for the sake of simplicity. For complete names see Annex A. This method applies to fertilizers containing chelates of one or more of the following micro-nutrients: cobalt, copper, iron, manganese, zinc and with a mass fraction of at least 0,1 %.
EN 13368-1:2014 is classified under the following ICS (International Classification for Standards) categories: 65.080 - Fertilizers. The ICS classification helps identify the subject area and facilitates finding related standards.
EN 13368-1:2014 has the following relationships with other standards: It is inter standard links to EN 13368-1:2001. Understanding these relationships helps ensure you are using the most current and applicable version of the standard.
You can purchase EN 13368-1:2014 directly from iTeh Standards. The document is available in PDF format and is delivered instantly after payment. Add the standard to your cart and complete the secure checkout process. iTeh Standards is an authorized distributor of CEN standards.
Standards Content (Sample)
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Düngemittel - Bestimmung von Chelatbildnern in Düngemitteln mit Chromatographie - Teil 1: Bestimmung von EDTA, HEEDTA und DTPA mit IonenchromatographieEngrais - Détermination des agents chélatants dans les engrais par chromatographie - Partie 1: Détermination du EDTA, HEEDTA et DTPA par chromatographie ioniqueFertilizers - Determination of chelating agents in fertilizers by chromatography - Part 1: Determination of EDTA, HEEDTA and DTPA by ion chromatography65.080GnojilaFertilizersICS:Ta slovenski standard je istoveten z:EN 13368-1:2014SIST EN 13368-1:2014en,fr,de01-marec-2014SIST EN 13368-1:2014SLOVENSKI
STANDARDSIST EN 13368-1:20021DGRPHãþD
EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 13368-1
January 2014 ICS 65.080 Supersedes EN 13368-1:2001English Version
Fertilizers - Determination of chelating agents in fertilizers by chromatography - Part 1: Determination of EDTA, HEEDTA and DTPA by ion chromatography
Engrais - Détermination des agents chélatants dans les engrais par chromatographie - Partie 1: Détermination du EDTA, HEEDTA et DTPA par chromatographie ionique
Düngemittel - Bestimmung von Chelatbildnern in Düngemitteln mit Chromatographie - Teil 1: Bestimmung von EDTA, HEEDTA und DTPA mit IonenchromatographieThis European Standard was approved by CEN on 16 November 2013.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre:
Avenue Marnix 17,
B-1000 Brussels © 2014 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 13368-1:2014 ESIST EN 13368-1:2014
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Die Norm EN 13368-1:2014 behandelt die Bestimmung von Chelatbildnern in Düngemitteln durch chromatographische Verfahren, insbesondere die Quantifizierung der Stoffe EDTA, HEEDTA und DTPA. Der Geltungsbereich dieser Europäischen Norm ist klar definiert und fokussiert sich auf die Analyse von Düngemitteln, die eines oder mehrere dieser Chelate enthalten. Dies ist besonders relevant für die Landwirtschaft, da viele Mikronährstoffe wie Kobalt, Kupfer, Eisen, Mangan und Zink in gebundener Form in Düngemitteln vorkommen und eine zuverlässige Analyse unerlässlich ist, um die Nährstoffverfügbarkeit zu optimieren. Ein entscheidender Vorteil dieser Norm liegt in ihrer Fähigkeit, die Gesamtmenge der wasserlöslichen Anteile der Chelate zu bestimmen. Diese Leistungsfähigkeit ist in der praktischen Anwendung von großer Bedeutung, da sie Landwirten und Herstellern hilft, fundierte Entscheidungen über die Nährstoffzusammensetzung ihrer Produkte zu treffen. Die Verwendung von Ionenchromatographie als Analysemethode bietet zudem eine hohe Präzision und Reproduzierbarkeit in den Ergebnissen. Ein weiterer Aspekt, der die Relevanz der Norm unterstreicht, ist die Tatsache, dass sie sich auf Chelate dieser spezifischen Mikronährstoffe konzentriert, die in der modernen Düngemittelherstellung häufig eingesetzt werden. Diese Fokussierung auf EDTA, HEEDTA und DTPA trägt zur Standardisierung von Analysemethoden in der Branche bei und fördert die Transparenz in den Produktspezifikationen. Allerdings ist zu beachten, dass die Norm nicht zwischen der freien Form und der metallgebundenen Form der Chelate unterscheidet, was in bestimmten Anwendungsszenarien eine Einschränkung darstellen kann. Dennoch bleibt die Norm ein essentielles Instrument für die Qualitätssicherung und Kontrolle von Düngemitteln, die auf den Einsatz von Chelatbildnern angewiesen sind. Zusammenfassend lässt sich sagen, dass die EN 13368-1:2014 eine wichtige Grundlage für die chromatographische Bestimmung von Chelatbildnern in Düngemitteln bietet, die sowohl die Analyseverfahren standardisiert als auch zur Steigerung der Effizienz in der Nährstoffnutzung in der Landwirtschaft beiträgt.
この標準文書、EN 13368-1:2014は、肥料に含まれるキレート剤EDTA、HEEDTA、DTPAのクロマトグラフィーを用いた定量に関する方法を規定しています。その範囲は、これらの物質が一つ以上含まれる肥料における各キレート剤の総量を明確に特定することに重点を置いています。この標準のメリットの一つは、対象となるキレート剤の水溶性分画を特定する能力にあります。 具体的には、EDTA、HEEDTA、DTPAといったキレート剤が含まれる肥料は、コバルト、銅、鉄、マンガン、亜鉛などの微量栄養素を含むことが多く、この標準によって、少なくとも0.1%の質量分率を持つ肥料が適用されます。標準は、肥料業界における品質管理や規制遵守にとって極めて重要で、その適用により製品の安全性と効果を保証することができます。 さらに、EDTA、HEEDTAおよびDTPAといった略語の使用は、文書の理解を容易にし、明確性を保つことに寄与しています。ただし、この方法は、キレート剤の遊離形態と金属結合形態の区別を許可しないため、注意が必要です。全体として、EN 13368-1:2014は、肥料製造業者において、キレート剤の分析を通じた品質保証を実現するための信頼性の高い指針を提供しており、肥料の安全性や環境への配慮を高める重要な役割を果たしています。
표준 EN 13368-1:2014은 비료 내에서 EDTA, HEEDTA 및 DTPA와 같은 킬레이트제의 함량을 크로마토그래피 방법으로 결정하는 절차를 상세히 규정하고 있습니다. 이 유럽 표준은 이러한 킬레이트제가 포함된 비료에서 각 개별 킬레이트제의 총량을 정확하게 파악할 수 있는 방법을 제공합니다. 특히, 이 표준의 범위는 EDTA, HEEDTA 및 DTPA의 수용성 비율을 측정하는 동시에, 각각의 킬레이트제가 자유 형태인지 금속으로 결합된 형태인지를 구분할 수 없다는 점을 명확히 하여 사용자가 이 방법의 제약을 이해할 수 있도록 합니다. 표준의 강점 중 하나는 불소미량의 미량원소인 코발트, 구리, 철, 망간, 아연과 같은 킬레이트계를 포함한 비료에서도 적용할 수 있다는 점입니다. 이는 다양한 비료 제품에서 널리 사용될 수 있는 유용한 분석 방법을 제공하며, 특히 사용자가 최소 0.1%의 질량 비율로 포함된 비료를 대상으로 이 방법을 활용할 수 있음을 의미합니다. 또한, 이 표준은 크로마토그래피라는 정확하고 신뢰할 수 있는 분석 기법을 통해 비료의 성분을 결정함으로써 생산자와 소비자 모두에게 높은 가치를 제공합니다. 이러한 정량적 데이터는 비료의 품질 확보를 위한 기초 자료가 되어, 지속 가능한 농업 실천에 기여하게 됩니다. 결론적으로, EN 13368-1:2014 표준은 비료 분석 및 품질 보증에 있어 매우 중요한 역할을 하며, 킬레이트제의 정확한 결정에 대한 요구 사항을 충족시키는 강력한 도구입니다. 비료 산업에서의 광범위한 적용 가능성 덕분에 이 표준은 현대 농업에서 더욱 중요한 의도를 갖게 됩니다.
The EN 13368-1:2014 standard presents a comprehensive framework for the determination of chelating agents, specifically EDTA, HEEDTA, and DTPA, in fertilizers using ion chromatography. Its scope is clearly defined, focusing on fertilizers that contain one or more of these chelating agents and ensuring accurate measurement of their total amounts. One of the significant strengths of this standard is its methodological rigor, which facilitates a reliable identification and quantification of the total water-soluble fraction of each of these chelating agents. This capability is crucial for manufacturers and regulators alike, as it ensures that products meet safety and efficacy requirements when marketed. Additionally, the specification of a minimum mass fraction of 0.1% for chelates containing essential micronutrients like cobalt, copper, iron, manganese, and zinc underscores the standard's relevance to the industry, promoting optimal nutrient availability for plant growth. While the standard effectively outlines the quantification method, it is worth noting the limitation that it does not differentiate between the free form and metal-bound forms of EDTA, HEEDTA, and DTPA. This aspect could be considered a constraint for users seeking a more granular understanding of the chemical nature of these chelating agents within fertilizers. Overall, the EN 13368-1:2014 standard serves as an essential tool for professionals in agricultural and environmental sectors, contributing significantly to the assurance of quality and compliance in fertilizers that utilize these vital chelating agents. Its detailed methodology and specific applicability make it a relevant and necessary reference for practitioners addressing the complexities of fertilizer composition.
La norme SIST EN 13368-1:2014 propose une méthode efficace pour la détermination chromatographique des agents chélatants EDTA, HEEDTA et DTPA dans les engrais. Son périmètre d'application inclut spécifiquement les engrais contenant ces substances, permettant ainsi une analyse précise de la fraction soluble dans l'eau de chacun de ces agents chélatants. Parmi les forces de cette norme, on note la rigueur méthodologique qui assure une identification claire des chélates. En outre, l'application vise les engrais qui contiennent des micro-nutriments essentiels tels que le cobalt, le cuivre, le fer, le manganèse et le zinc, ce qui en fait un outil pertinent pour les producteurs et les distributeurs d'engrais. La possibilité de quantifier la totalité des agents chélatants dans les formulations d'engrais répond à un besoin croissant de transparence et de qualité dans l'industrie des fertilisants. Cependant, il est important de rappeler que cette norme ne permet pas de faire la distinction entre les formes libres et liées des chélates, un aspect qui pourrait avoir des implications sur la disponibilité des nutriments pour les plantes. Malgré cette limitation, la norme reste d'un grand intérêt pour ceux qui souhaitent respecter des réglementations européennes en matière de fertilisation et garantir la sécurité et l'efficacité de leurs produits. Dans l'ensemble, la norme SIST EN 13368-1:2014 représente une avancée significative pour la normalisation des méthodes d'analyse des engrais contenant EDTA, HEEDTA et DTPA, renforçant ainsi l'importance des chélatants dans la nutrition des plantes et la protection de l'environnement. Son adoption par les acteurs de l'industrie est donc fortement recommandée.
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