Plant biostimulants - Detection of Vibrio spp.

This document specifies a horizontal method for the detection of enteropathogenic Vibrio spp., which causes human illness in or via the intestinal tract [1]. The species detectable by the methods specified include Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus.
It is applicable to the following:
—   microbial plant biostimulants.
NOTE 1   The World Health Organization (WHO) has identified that V. parahaemolyticus, V. cholerae and V. vulnificus are the major contaminants of Vibrio spp. [1].
NOTE 2   For confirmation, it is possible to use PCR tests; in this case the laboratory validates the procedure and data generated.

Pflanzen-Biostimulanzien - Nachweis von Vibrio spp.

Dieses Dokument legt ein horizontales Verfahren zum Nachweis von enteropathogenen Vibrio spp. fest, die Erkrankungen des Menschen am oder über den Darmtrakt verursachen [1]. Die Spezies, deren Nachweis die hier aufgeführten Verfahren einbeziehen, sind Vibrio parahaemolyticus, Vibrio cholerae und Vibrio vulnificus.
Das Dokument gilt für
—   mikrobielle Pflanzen Biostimulanzien.
ANMERKUNG 1   Die Weltgesundheitsorganisation (WHO; en: World Health Organization) hat V. parahaemolyticus, V. cholerae und V. vulnificus als die wichtigsten Kontaminanten von Vibrio spp. identifiziert [1].
ANMERKUNG 2   Eine Bestätigung durch PCR Tests ist möglich; in diesem Fall validiert das Labor das Verfahren und die erzeugten Daten.

Biostimulants des végétaux - Recherche des espèces de Vibrio

Le présent document spécifie une méthode horizontale pour la recherche des espèces entéropathogènes de Vibrio provoquant des maladies dans ou via le tractus intestinal chez l’homme [1]. Les espèces détectables par les méthodes spécifiées incluent Vibrio parahaemolyticus, Vibrio cholerae et Vibrio vulnificus.
Il s’applique :
—   aux biostimulants microbiens des végétaux.
NOTE 1   L’Organisation Mondiale de la Santé (OMS) a identifié V. parahaemolyticus, V. cholerae et V. vulnificus comme les principales espèces de Vibrio contaminantes [1].
NOTE 2   Pour la confirmation, il est possible de recourir aux essais de PCR ; dans ce cas, le laboratoire valide le mode opératoire et les données générées.

Rastlinski biostimulanti - Ugotavljanje prisotnosti Vibrio spp.

Ta dokument določa vodoravno metodo za ugotavljanje prisotnosti enteropatogenih bakterij Vibrio spp., ki povzročajo bolezni v prebavilih ali prek prebavil ljudi [1]. Vrste bakterij, ki jih je mogoče zaznati z navedenimi metodami, vključujejo Vibrio parahaemolyticus, Vibrio cholerae in Vibrio vulnificus.
Uporablja se za:
–   mikrobne rastlinske biostimulante.
OPOMBA 1:   Svetovna zdravstvena organizacija (SZO) je ugotovila, da so V. parahaemolyticus, V. cholerae in V. vulnificus glavni onesnaževalci Vibrio spp. [1].
OPOMBA 2:   Za potrditev je možna uporaba preskusov PCR; v tem primeru laboratorij validira postopek in pridobljene podatke.

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Standards Content (Sample)

SIST-TS CEN/TS 17711:2023
Rastlinski biostimulanti - Ugotavljanje prisotnosti Vibrio spp.
Plant biostimulants - Detection of Vibrio spp
Pflanzen-Biostimulanzien - Nachweis von Vibrio spp.
Biostimulants des végétaux - Recherche des espèces de Vibrio
Ta slovenski standard je istoveten z: CEN/TS 17711:2022
65.080 Gnojila Fertilizers
SIST-TS CEN/TS 17711:2023 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST-TS CEN/TS 17711:2023

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SIST-TS CEN/TS 17711:2023

CEN/TS 17711


March 2022
ICS 65.080
English Version

Plant biostimulants - Detection of Vibrio spp.
Biostimulants des végétaux - Recherche des espèces de Pflanzen-Biostimulanzien - Nachweis von Vibrio spp.
This Technical Specification (CEN/TS) was approved by CEN on 3 January 2022 for provisional application.

The period of validity of this CEN/TS is limited initially to three years. After two years the members of CEN will be requested to
submit their comments, particularly on the question whether the CEN/TS can be converted into a European Standard.

CEN members are required to announce the existence of this CEN/TS in the same way as for an EN and to make the CEN/TS
available promptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in
parallel to the CEN/TS) until the final decision about the possible conversion of the CEN/TS into an EN is reached.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.



CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2022 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN/TS 17711:2022 E
worldwide for CEN national Members.

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SIST-TS CEN/TS 17711:2023
CEN/TS 17711:2022 (E)
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle. 6
4.1 General . 6
4.2 Primary enrichment in a liquid selective medium. 6
4.3 Secondary enrichment in a liquid selective medium . 6
4.4 Isolation and identification . 6
4.5 Confirmation . 7
5 Culture media and reagents . 7
5.1 Enrichment medium: alkaline saline peptone water (ASPW) . 7
5.2 Solid selective isolation media . 7
5.3 Saline nutrient agar (SNA) . 8
5.4 Reagent for detection of oxidase . 8
5.5 Biochemi

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