Soil quality - In situ caging of snails to assess bioaccumulation of contaminants (ISO 24032:2021)

This document describes a method to assess the bioaccumulation of chemicals in snails, i.e. concentrations of metal(loid)s (ME) or organic compounds [e.g. polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs)] accumulated in their tissues.
This document presents how to prepare snails for caging in situ for 28 days, the in situ test design and then how to collect and prepare the snails until conservation and further analysis. If a kinetic study of accumulation is necessary, sampling of snails at different time-points during exposure is possible as well [13],[19],[22].
This document excludes analytical methods. Preparation (extraction and mineralization) of the samples and quantification of chemicals are not in the scope of the present document.
The method is applicable for soils under different uses (agricultural, industrial, residential, forests, before and after remediation, on potentially contaminated sites, etc.) and waste materials [8],[10], preferably with vegetation and/or humus cover.
The method is applicable subject to certain limits of temperature (frost-free period, i.e. mainly from April to October in temperate region).
Optionally (see Annex I), the method can be used in the laboratory to evaluate the accumulation of contaminants [and optionally, the sum of excess of transfer (SET) index for ME, PAH, PCB] of snails exposed only to soil.

Bodenbeschaffenheit - In-situ-Käfighaltung von Schnecken zur Beurteilung der Bioakkumulation von Kontaminanten (ISO 24032:2021)

Dieses Dokument beschreibt ein Verfahren zur Bewertung der Bioakkumulation von Chemikalien in Schnecken, d. h. Konzentrationen von Metall(oid)en (ME) oder organischen Verbindungen, z. B. polyzyklische aromatische Kohlenwasserstoffe (PAK) und polychlorierte Biphenyle (PCB), die sich in ihren Geweben angereichert haben.
In diesem Dokument wird dargelegt, wie Schnecken für die 28 tägige In situ-Käfighaltung vorbereitet werden, wie das In situ-Prüfdesign aussieht und wie die Schnecken bis zur Konservierung und weiteren Analyse gesammelt und vorbereitet werden. Falls eine kinetische Untersuchung der Akkumulation notwendig ist, ist auch eine Probenahme der Schnecken zu verschiedenen Zeitpunkten während der Exposition möglich [13] [19] [22].
Dieses Dokument schließt analytische Verfahren aus. Die Vorbereitung (Extraktion und Mineralisierung) der Proben und Quantifizierung von Chemikalien fallen nicht in den Anwendungsbereich des vorliegenden Dokuments.
Das Verfahren ist auf Böden mit unterschiedlicher Nutzung (landwirtschaftliche, industrielle, Wohn  und Waldböden, vor und nach der Sanierung, auf potenziell kontaminierten Standorten usw.) sowie auf Abfallmaterialien [8] [10] anwendbar, vorzugsweise auf Böden mit Vegetation und/oder Humusbedeckung.
Das Verfahren ist vorbehaltlich bestimmter Temperaturgrenzen (frostfreier Zeitraum, d. h. hauptsächlich von April bis Oktober in gemäßigtem Klima) anwendbar.
Optional (siehe Anhang I) kann das Verfahren im Labor verwendet werden, um die Akkumulation von Kontaminanten (und optional die Summe des Übertragungsüberschusses (en: Sum of Excess of Transfer) (SET) index für ME, PAK, PCB) von Schnecken zu bewerten, die nur dem Boden exponiert sind.

Qualité du sol - Encagement in situ d’escargots pour la mesure de la bioaccumulation de contaminants (ISO 24032:2021)

Le présent document décrit une méthode permettant d’évaluer la bioaccumulation de substances chimiques chez les escargots, c’est-à-dire les concentrations de métaux (métalloïdes) (ME) ou de composés organiques [tels que les hydrocarbures aromatiques polycycliques (HAP) et les polychlorobiphényles (PCB)] accumulés dans leurs tissus.
Le présent document présente la méthodologie pour préparer les escargots pour un encagement in situ pendant 28 jours, la description de l’essai in situ, puis la méthodologie de collecte et de préparation des escargots jusqu’à leur conservation pour analyse ultérieure. Si une étude cinétique de l’accumulation est nécessaire, il est également possible de prélever des échantillons d’escargots à différents moments de l’exposition[13],[19],[22].
Le présent document exclut les méthodes analytiques. La préparation (extraction et minéralisation) des échantillons et la quantification des substances chimiques ne font pas partie du domaine d’application du présent document.
La méthode est applicable à des sols destinés à différentes utilisations (agricole, industrielle, résidentielle, forestière, avant et après dépollution, sur des sites potentiellement pollués, etc.) et aux déchets[8],[10], de préférence sur des sols recouverts d’une couverture végétale et/ou d’une couche d’humus.
La méthode est applicable sous réserve de certaines limites de température (période sans gel, c’est-à-dire généralement d’avril à octobre en région tempérée).
En option (voir Annexe I), la méthode peut être utilisée en laboratoire pour évaluer l’accumulation de contaminants [et, facultativement, l’indice Somme des excès de transfert (SET) pour les ME, HAP et PCB] des escargots exposés uniquement au sol.

Kakovost tal - Uporaba kletk s polži na terenu za oceno bioakumulacije onesnaževal (ISO 24032:2021)

Ta dokument opisuje metodo za ocenjevanje bioakumulacije kemikalij v polžih, tj. koncentracij kovin in metaloidov (ME) oziroma organskih spojin [npr. policikličnih aromatskih ogljikovodikov (PAH) in polikloriranih bifenilov (PCB)], nakopičenih v njihovih tkivih. V tem dokumentu je predstavljeno, kako pripraviti polže za 28-dnevno nastanitev v kletke na kraju samem, vključno z načrtom preskusa na kraju samem ter nadalje s postopkom zbiranja in priprave polžev do skladiščenja oziroma nadaljnje analize. Če je potrebna kinetična študija akumulacije, je mogoče izvesti tudi vzorčenje polžev ob različnem času med izpostavljenostjo [13], [19], [22]. Ta dokument vključuje analitične metode. Priprava (ekstrakcija in mineralizacija) vzorcev in količinska opredelitev kemikalij ne spadata na področje uporabe tega dokumenta. Metoda se uporablja za tla v okviru različnih vrst uporabe (kmetijska, industrijska ali stanovanjska uporaba, gozdovi, pred sanacijo in po njej, na potencialno onesnaženih območjih itd.) in odpadne materiale [8], [10], po možnosti z rastlinjem in/ali humusom. Metoda se uporablja ob upoštevanju določenih omejitev glede temperature (obdobje brez zmrzali, tj. od aprila do oktobra v območjih z zmernim podnebjem). Metodo je mogoče izbirno (glej dodatek I) uporabiti v laboratoriju za vrednotenje akumulacije onesnaževal [in izbirno indeksa vsote presežnega prenosa (SET) za kovine in metaloide, policiklične aromatske ogljikovodike ter poliklorirane bifenile] v polžih, izpostavljenih izključno tlom.

General Information

Status
Published
Publication Date
14-Dec-2021
Withdrawal Date
29-Jun-2022
Current Stage
6060 - Definitive text made available (DAV) - Publishing
Start Date
15-Dec-2021
Due Date
24-Apr-2023
Completion Date
15-Dec-2021

Buy Standard

Standard
EN ISO 24032:2022 - BARVE
English language
67 pages
sale 10% off
Preview
sale 10% off
Preview
e-Library read for
1 day
Draft
prEN ISO 24032:2021 - BARVE
English language
53 pages
sale 10% off
Preview
sale 10% off
Preview
e-Library read for
1 day

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 24032:2022
01-marec-2022
Kakovost tal - Uporaba kletk s polži na terenu za oceno bioakumulacije
onesnaževal (ISO 24032:2021)
Soil quality - In situ caging of snails to assess bioaccumulation of contaminants (ISO
24032:2021)
Bodenbeschaffenheit - In-situ-Käfighaltung von Schnecken zur Beurteilung der
Bioakkumulation von chemischen Stoffen (ISO 24032:2021)
Qualité du sol - Encagement in situ d’escargots pour la mesure de la bioaccumulation de
contaminants (ISO 24032:2021)
Ta slovenski standard je istoveten z: EN ISO 24032:2021
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
SIST EN ISO 24032:2022 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
SIST EN ISO 24032:2022

---------------------- Page: 2 ----------------------
SIST EN ISO 24032:2022


EN ISO 24032
EUROPEAN STANDARD

NORME EUROPÉENNE

December 2021
EUROPÄISCHE NORM
ICS 13.080.30
English Version

Soil quality - In situ caging of snails to assess
bioaccumulation of contaminants (ISO 24032:2021)
Qualité du sol - Encagement in situ d'escargots pour la Bodenbeschaffenheit - In-situ-Käfighaltung von
mesure de la bioaccumulation de contaminants (ISO Schnecken zur Beurteilung der Bioakkumulation von
24032:2021) chemischen Stoffen (ISO 24032:2021)
This European Standard was approved by CEN on 5 December 2021.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2021 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 24032:2021 E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------
SIST EN ISO 24032:2022
EN ISO 24032:2021 (E)
Contents Page
European foreword . 3

2

---------------------- Page: 4 ----------------------
SIST EN ISO 24032:2022
EN ISO 24032:2021 (E)
European foreword
This document (EN ISO 24032:2021) has been prepared by Technical Committee ISO/TC 190 "Soil
quality" in collaboration with Technical Committee CEN/TC 444 “Environmental characterization of
solid matrices” the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by June 2022, and conflicting national standards shall be
withdrawn at the latest by June 2022.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 24032:2021 has been approved by CEN as EN ISO 24032:2021 without any modification.

3

---------------------- Page: 5 ----------------------
SIST EN ISO 24032:2022

---------------------- Page: 6 ----------------------
SIST E
...

SLOVENSKI STANDARD
oSIST prEN ISO 24032:2021
01-januar-2021
Kakovost tal - Uporaba kletk s polži na terenu za oceno bioakumulacije
onesnaževal (ISO/DIS 24032:2020)
Soil quality - In situ caging of snails to assess bioaccumulation of contaminants (ISO/DIS
24032:2020)
Bodenbeschaffenheit - In-situ-Käfighaltung von Schnecken zur Beurteilung der
Bioakkumulation von chemischen Stoffen (ISO/DIS 24032:2020)
Qualité des sols - Encagement in situ descargots pour la mesure de la bioaccumulation
de contaminants (ISO/DIS 24032:2020)
Ta slovenski standard je istoveten z: prEN ISO 24032
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
oSIST prEN ISO 24032:2021 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
oSIST prEN ISO 24032:2021

---------------------- Page: 2 ----------------------
oSIST prEN ISO 24032:2021
DRAFT INTERNATIONAL STANDARD
ISO/DIS 24032
ISO/TC 190/SC 4 Secretariat: AFNOR
Voting begins on: Voting terminates on:
2020-11-18 2021-02-10
Soil quality — In situ caging of snails to assess
bioaccumulation of contaminants
Qualité du sol — Encagement in situ d’escargots pour la mesure de la bioaccumulation de contaminants
ICS: 13.080.30
THIS DOCUMENT IS A DRAFT CIRCULATED
This document is circulated as received from the committee secretariat.
FOR COMMENT AND APPROVAL. IT IS
THEREFORE SUBJECT TO CHANGE AND MAY
NOT BE REFERRED TO AS AN INTERNATIONAL
STANDARD UNTIL PUBLISHED AS SUCH.
IN ADDITION TO THEIR EVALUATION AS
ISO/CEN PARALLEL PROCESSING
BEING ACCEPTABLE FOR INDUSTRIAL,
TECHNOLOGICAL, COMMERCIAL AND
USER PURPOSES, DRAFT INTERNATIONAL
STANDARDS MAY ON OCCASION HAVE TO
BE CONSIDERED IN THE LIGHT OF THEIR
POTENTIAL TO BECOME STANDARDS TO
WHICH REFERENCE MAY BE MADE IN
Reference number
NATIONAL REGULATIONS.
ISO/DIS 24032:2020(E)
RECIPIENTS OF THIS DRAFT ARE INVITED
TO SUBMIT, WITH THEIR COMMENTS,
NOTIFICATION OF ANY RELEVANT PATENT
RIGHTS OF WHICH THEY ARE AWARE AND TO
©
PROVIDE SUPPORTING DOCUMENTATION. ISO 2020

---------------------- Page: 3 ----------------------
oSIST prEN ISO 24032:2021
ISO/DIS 24032:2020(E)

COPYRIGHT PROTECTED DOCUMENT
© ISO 2020
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii © ISO 2020 – All rights reserved

---------------------- Page: 4 ----------------------
oSIST prEN ISO 24032:2021
ISO/DIS 24032:2020(E)

Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Test organism and equipment . 2
5.1 Biological material . 2
5.2 Equipment .
...

Questions, Comments and Discussion

Ask us and Technical Secretary will try to provide an answer. You can facilitate discussion about the standard in here.