EN ISO 11214:1996

Modified starch - Determination of carboxyl group content of oxidized starch (ISO 11214:1996)

Migrated from Progress Sheet (TC Comment) (2000-07-10): According to a PC4 recommendation, CEN/BT agreed to launch a parallel voting ++ procedure on the draft international standard derived from the above ++ mentionned subject (Ref.nr. in ISO 11214).

Amidon modifié - Dosage des groupes carboxyles dans l'amidon oxydé (ISO 11214:1996)

La présente Norme internationale prescrit une méthode pour le dosage des groupes carboxyles dans l'amidon oxydé. La méthode convient au dosage des groupes carboxyles jusqu'à une teneur de 1 % (m/m).

Modificiran škrob - Določevanje karboksilnih skupin v oksidiranem škrobu (ISO 11214:1996)

General Information

• Status: Published
• Publication Date: 30-Jun-1996
• Withdrawal Date: 30-Oct-1997
• ICS: 67.180.20 - Starch and derived products
• Technical Committee: CEN/SS C10 - Starch
• Drafting Committee: CEN/SS C10 - Starch
• Current Stage: 9093 - Decision to confirm - Review Enquiry
• Start Date: 24-Jan-2001
• Completion Date: 24-Jan-2001
• Ref Project: SIST EN ISO 11214:1998 - Modified starch - Determination of carboxyl group content of oxidized starch (ISO 11214:1996)

Overview

EN ISO 11214:1996 is an international standard that specifies a precise analytical method for determining the carboxyl group content in oxidized starch. Published by CEN as a parallel adoption of ISO 11214:1996, this standard is essential for quality control and research in starch modification processes. It focuses on measuring carboxyl groups up to 1% (m/m) in oxidized starch, a critical parameter influencing starch properties in industrial applications.

Key Topics

• Method Scope: Defines a titrimetric procedure for quantifying carboxyl groups in oxidized starch, suitable for concentrations up to 1% (mass/mass).
• Sample Preparation: Includes milling, sieving, and optional defatting of starch samples to ensure homogeneous and representative test material.
• Reagents and Apparatus: Uses specific graded chemicals such as hydrochloric acid, sodium hydroxide, phenolphthalein indicator, and silver nitrate, alongside laboratory essentials like magnetic stirrers, vacuum filtration setups, and boiling water baths.
• Test Procedure:
  • Conversion of carboxyl salts to acid form by hydrochloric acid
  • Removal of excess acid and cations through thorough washing
  • Gelatinization of starch to facilitate titration
  • Titration with NaOH to an endpoint indicated by phenolphthalein or electrometric detection
• Corrections for Specific Starches: For oxidized potato starch, phosphate content is determined and results are corrected accordingly. For maize and wheat starch, potential lipid interference is addressed through Soxhlet defatting or adjustment of carboxyl content values.
• Result Expression and Calculation: Provides a formula that includes the titration volume, concentration, and moisture content to express carboxyl content on a dry weight basis, ensuring standardized results.
• Precision and Reproducibility: Guarantees reliable results with repeatability limits within 6% and reproducibility within 16% based on interlaboratory studies.

Applications

EN ISO 11214:1996 serves diverse industries where modified starches are used or produced, including:

• Food Industry: Monitoring starch oxidation levels in baking, thickening agents, and texture modification for safety and performance consistency.
• Paper and Textile Manufacturing: Quality control of oxidized starch used as surface sizing and warp sizing agents.
• Pharmaceuticals and Bioplastics: Validating starch modification which impacts biodegradability, drug delivery, and material properties.
• Research and Development: Providing a standardized method for scientists and product developers to characterize oxidized starches in innovative formulations.

Related Standards

• ISO 1666: Specifies the oven-drying method for moisture content determination in starch, crucial for accurate dry-mass calculations in EN ISO 11214.
• ISO 3696: Defines water purity for analytical laboratory use, ensuring that water quality does not affect titration results.
• ISO 3946: Details the spectrophotometric method for total phosphorus determination in starch products, used for correcting phosphate interference in oxidized potato starch measurements.
• ISO 5725-1 and ISO 5725-2: Provide guidelines on measurement accuracy, repeatability, and reproducibility used to validate the precision of the EN ISO 11214 test method.

Summary

EN ISO 11214:1996 is a critical standard for accurate determination of carboxyl group content in oxidized starch. By following its detailed procedure, laboratories and industries can ensure reliable quality assessment of modified starches, enabling consistent product performance and regulatory compliance. The standard’s integration with related ISO standards further supports comprehensive starch characterization, reinforcing its practical significance across multiple applications in starch chemistry and product development.

Standards Content (Sample)

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Amidon modifié - Dosage des groupes carboxyles dans l'amidon oxydé (ISO 11214:1996)Modified starch - Determination of carboxyl group content of oxidized starch (ISO 11214:1996)67.180.20Škrob in izdelki iz njegaStarch and derived productsICS:Ta slovenski standard je istoveten z:EN ISO 11214:1996SIST EN ISO 11214:1998en01-november-1998SIST EN ISO 11214:1998SLOVENSKI
STANDARD
INTERNATIONAL STANDARD IS0 11214 First edition 1996-07-01 Modified starch - Determination of carboxyl group content of oxidized starch Amidon modifi6 - Dosage des groupes carboxyles dans I’amidon oxyd6 Reference number IS0 11214:1996(E) SIST EN ISO 11214:1998

IS0 11214:1996(E) Foreword IS0 (the International Organization for Standardization) is a worldwide federation of national standards bodies (IS0 member bodies). The work of preparing International Standards is normally carried out through IS0 technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. IS0 collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. International Standard IS0 11214 was prepared by Technical Committee lSO/TC 93, Starch (including derivatives and by-products). Annexes A and B of this International Standard are for information only. 0 IS0 1996 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from the publisher. International Organization for Standardization Case Postale 56 l CH-121 1 Geneve 20 l Swi tzerland Printed in Switzerland SIST EN ISO 11214:1998

INTERNATIONAL STANDARD 0 ISO ISO 11214:1996(E) Modified starch - Determination of carboxyl group content of oxidized starch 1 Scope This International Standard specifies a method for the determination of the carboxyl group content of oxidized starch. The method is suitable for determining carboxyl group contents up to 1 % (m/m). 2 Normative references The following standards contain provisions which, through reference in this text, constitute provisions of this International Standard. At the time of publi- cation, the editions indicated were valid. All standards are subject to revision, and parties to agreements based on this International Standard are encouraged to investigate the possibility of applying the most re- cent editions of the standards indicated below. Members of IEC and IS0 maintain registers of cur- rently valid International Standards. IS0 1666: --J), Starch - Determination of moisture content - Oven-drying method. I SO 3696: 1987, Water for analytical labora tory use - Specification and test methods. IS0 3946:1982, Starches and derived products - Determination of total phosphorus content - Spectrophotometric method. 3 Principle The carboxyl groups are converted into the acid form by adding a mineral acid to a homogenized test por- tion of the oxidized starch. The cations and the excess acid are eliminated by washing with water. The washed sample is gelatinized and titrated with sodium hydroxide standard solution. For oxidized potato starch, the result is corrected for the phosphate group content. 4 Reagents and materials Use only reagents of recognized analytical grade, un- less otherwise specified, and water complying with grade 2 in accordance with IS0 3696. The water used shall be free of carbon dioxide. 4.1 Hydrochloric acid, 0,l mol/l solution. 4.2 Sodium hydroxide, standard volumetric sol- ution, c(NaOH) = 0,l mol/l, free of carbon dioxide. 4.3 Phenolphthalein in ethanol, 1 g/l solution,- 90 % (VW). 4.4 Silver nitrate, 10 g/l solution. 5 Apparatus Usual laboratory apparatus and in particular the fol- lowing. 5.1 Beakers, of capacity 100 ml and 600 ml. 5.2 Magnetic stirrer. 1) To be published. (Revision of IS0 1666:1973) SIST EN ISO 11214:1998

IS0 11214:1996(E) 0 IS0 5.3 Vacuum filter, equipped with a fritted glass crucible of medium porosity, or a Buchner funnel, of diameter 55 mm, equipped with a small, round filter paper (medium filtration rate). 5.4 Boiling water bath. 5.5 Mechanical stirrer. 5.6 Blade mill. 5.7 Test sieve, of aperture size 800 pm. 6 Preparation of the test sample Pass the sample through the test sieve (5.7). If the sample does not pass through the sieve, grind it using the blade mill (5.6) so that it completely passes through the sieve. Homogenize the sample. NOTE 1 In the case of oxidized maize or wheat starch, it may be desirable to defat the sample by Soxhlet extraction with a mixture of propanol and water [3 + 1 (V/V)] in order to correct for the contribution of lipids to the carboxyl group content. 7 Procedure 7.1 Test portion Weigh, to the nearest 0,l mg, 5 g of the test sample into a 100 ml beaker (5.1). 7.2 Conversion of the carboxyl salts Add 25 ml of the hydrochloric acid solution (4. I) to the beaker and stir for 30 min on the magnetic stirrer (5.2). 7.3 Washing Filter the suspension under vacuum through a fritted glass crucible or Btichn
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