EN ISO 7704:2023

Water quality - Requirements for the performance testing of membrane filters used for direct enumeration of microorganisms by culture methods (ISO 7704:2023)

This document specifies the requirements for the performance testing of membrane filters used for the retention followed by direct enumeration of microorganisms by culture methods.
This document is applicable to membrane filters which are used for retention followed by direct enumeration of specific microorganisms on solid media or on other devices containing media, like absorbent pads[19].
This document is not applicable for membrane filters used for concentration and elution or for qualitative methods.
These tests are applicable to the membrane filters intended for the microbiological analysis of different types of water, such as:
—    drinking water, bottled water and other types of water with expected low numbers of microorganisms;
—    water with expected higher numbers of microorganisms, for example, surface water and process water.
These tests are intended to demonstrate the suitability of the whole system (membrane filter together with the culture medium including the filtration step) required for the specific tests described in References [3], [6], [8], [10], [12] and [13].
This document applies to:
—    manufacturers producing membrane filters;
—    microbiological laboratories using membrane filters for their own testing or providing these to other end users.

Wasserbeschaffenheit - Anforderungen für die Bewertung von Membranfiltern zur direkten Zählung mittels mikrobiologischer Kulturverfahren (ISO 7704:2023)

Dieses Dokument legt die Anforderungen an die Leistungsprüfung von Membranfiltern fest, die für die Rückhaltung und die anschließende direkte Zählung von Mikroorganismen durch Verfahren zur Kultivierung verwendet werden.
Dieses Dokument ist anwendbar für Membranfilter, die für die Rückhaltung und anschließende direkte Zählung bestimmter Mikroorganismen auf festen Medien oder auf anderen Vorrichtungen, die Medien enthalten, wie absorbierendes Trägermaterial, verwendet werden [19].
Dieses Dokument ist nicht anwendbar für Membranfilter, die zur Aufkonzentrierung und Elution oder für qualitative Verfahren verwendet werden.
Die Prüfungen sind anwendbar für Membranfilter, die für die mikrobiologische Analyse verschiedener Arten von Wasser bestimmt sind, wie:
   Trinkwasser, in Flaschen abgefülltes Wasser und andere Arten von Wasser mit voraussichtlich geringer Anzahl von Mikroorganismen;
   Wasser mit einer erwarteten höheren Anzahl von Mikroorganismen, z. B. Oberflächenwasser und Prozesswasser.
Die Prüfungen sollen die Eignung des gesamten Systems (Membranfilter zusammen mit dem Nährmedium einschließlich der Filtrationsstufe) nachweisen, die für die in den Literaturhinweisen [3] [6] [8] [10] [12] und [13] beschriebenen spezifischen Prüfungen erforderlich ist.
Dieses Dokument gilt für:
   Hersteller, die Membranfilter produzieren;
   Laboratorien für Mikrobiologie, die Membranfilter für ihre eigenen Prüfungen verwenden oder diese an andere Endanwender liefern.

Qualité de l'eau - Exigences relatives aux essais de performance des membranes filtrantes utilisées pour le dénombrement direct des micro-organismes par des méthodes de culture (ISO 7704:2023)

Le présent document spécifie les exigences pour les essais de performance des membranes filtrantes utilisées pour la rétention suivie d'un dénombrement direct des micro-organismes par des méthodes de culture.
Le présent document s'applique aux membranes filtrantes qui sont utilisées pour la rétention suivie d'un dénombrement direct de micro-organismes spécifiques sur des milieux solides ou sur d'autres dispositifs contenant des milieux, comme des pads en papier absorbants [19].
Le présent document ne s'applique pas aux membranes filtrantes utilisées pour la concentration et l'élution ou pour des méthodes qualitatives.
Les essais s'appliquent aux membranes filtrantes destinées à l'analyse microbiologique de différents types d'eau, tels que:
—    l'eau potable, l'eau embouteillée et d'autres types d'eau dans laquelle un faible nombre de micro-organismes est attendu;
—    l'eau dans laquelle un plus grand nombre de micro-organismes est attendu, par exemple l'eau de surface et l'eau de process.
Ces essais sont destinés à démontrer l'adéquation de l'ensemble du système (membrane filtrante et milieu de culture, y compris l'étape de filtration) requis pour les essais spécifiques décrits dans les Références [3], [6], [8], [10], [12] et [13].
Le présent document s'applique:
—    aux fabricants produisant des membranes filtrantes;
—    aux laboratoires de microbiologie utilisant des membranes filtrantes pour leurs propres essais ou les fournissant à d'autres utilisateurs finaux.

Kakovost vode - Zahteve za preskušanje lastnosti membranskih filtrov, ki se uporabljajo za štetje mikroorganizmov z metodami kultivacije (ISO 7704:2023)

Ta mednarodni standard določa zahteve za oceno membranskih filtrov za koncentriranje za neposredno štetje specifičnih mikroorganizmov in mešanih populacij. Te zahteve veljajo za vse membranske filtre, namenjene za mikrobiološko analizo vseh vrst vzorcev vode in drugih tekočin. Te zahteve so namenjene za preskus, s katerim se dokaže ustreznost celotnega sistema – membranski filter z medijem za gojenje kulture, vključno s fazo filtriranja – potrebnega za določene preskuse, opisane v mednarodnih standardih.  Mehanski filtri, zahtevani za uporabo, so opisani v posameznih standardih. Ta mednarodni standard se uporablja za proizvajalce in uporabnike, kot so: – komercialni organi, ki proizvajajo in/ali distribuirajo membranske filtre;
– nekomercialni organi, ki membranske filtre dobavljajo tretjim strankam;
– mikrobiološki laboratoriji, ki uporabljajo membranske filtre za lastno preskušanje ali jih zagotavljajo drugim uporabnikom.

General Information

• Status: Published
• Publication Date: 24-Jan-2023
• ICS: 07.100.20 - Microbiology of water
• Technical Committee: CEN/TC 230 - Water analysis
• Drafting Committee: CEN/TC 230 - Water analysis
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 25-Jan-2023
• Completion Date: 25-Jan-2023
• Ref Project: SIST EN ISO 7704:2023 - Water quality - Requirements for the performance testing of membrane filters used for direct enumeration of microorganisms by culture methods (ISO 7704:2023)

Standards Content (Sample)

SLOVENSKI STANDARD
01-junij-2023
Kakovost vode - Zahteve za preskušanje lastnosti membranskih filtrov, ki se
uporabljajo za štetje mikroorganizmov z metodami kultivacije (ISO 7704:2023)
Water quality - Requirements for the performance testing of membrane filters used for
direct enumeration of microorganisms by culture methods (ISO 7704:2023)
Wasserbeschaffenheit - Anforderungen für die Bewertung von Membranfiltern zur
direkten Zählung mittels mikrobiologischer Kulturverfahren (ISO 7704:2023)
Qualité de l'eau - Exigences relatives aux essais de performance des membranes
filtrantes utilisées pour le dénombrement direct des micro-organismes par des méthodes
de culture (ISO 7704:2023)
Ta slovenski standard je istoveten z: EN ISO 7704:2023
ICS:
07.100.20 Mikrobiologija vode Microbiology of water
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 7704
EUROPEAN STANDARD
NORME EUROPÉENNE
January 2023
EUROPÄISCHE NORM
ICS 07.100.20
English Version
Water quality - Requirements for the performance testing
of membrane filters used for direct enumeration of
microorganisms by culture methods (ISO 7704:2023)
Qualité de l'eau - Exigences relatives aux essais de Wasserbeschaffenheit - Anforderungen für die
performance des membranes filtrantes utilisées pour Bewertung von Membranfiltern zur direkten Zählung
le dénombrement direct des micro-organismes par des mittels mikrobiologischer Kulturverfahren (ISO
méthodes de culture (ISO 7704:2023) 7704:2023)
This European Standard was approved by CEN on 19 November 2022.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2023 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 7704:2023 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 7704:2023) has been prepared by Technical Committee ISO/TC 147 "Water
quality" in collaboration with Technical Committee CEN/TC 230 “Water analysis” the secretariat of
which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by July 2023, and conflicting national standards shall be
withdrawn at the latest by July 2023.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
United Kingdom.
Endorsement notice
The text of ISO 7704:2023 has been approved by CEN as EN ISO 7704:2023 without any modification.

INTERNATIONAL ISO
STANDARD 7704
Second edition
2023-01
Water quality — Requirements for
the performance testing of membrane
filters used for direct enumeration of
microorganisms by culture methods
Qualité de l'eau — Exigences relatives aux essais de performance
des membranes filtrantes utilisées pour le dénombrement direct des
micro-organismes par des méthodes de culture
Reference number
ISO 7704:2023(E)
ISO 7704:2023(E)
© ISO 2023
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: [phone]
Email: [e-mail]
Website: www.iso.org
Published in Switzerland
ii
ISO 7704:2023(E)
Contents Page
Foreword .v
Introduction .vii
1 S c op e . 1
2 Nor m at i ve r ef er enc e s . 1
3 T erms and definitions . 1
3.1 G eneral terminology . 2
3.2 T erminology of performance testing . 3
3.3 T erminology for test microorganisms . 4
4 P r i nc iple . 5
4.1 General . 5
4 .1.1 I nt r o duc t ion . 5
4.1.2 Batch testing . 6
4.1.3 Supplementary testing . 6
4.2 P erformance testing . 7
4.2.1 M odules for batch and supplementary testing . 7
4.2.2 A bsence of microbial contamination . 7
5 A pparatus and glassware .8
6 C ulture media and diluents .8
7 P reparation of microorganisms for performance testing . 8
7.1 G eneral . 8
7. 2 R ef er enc e c ou nt . 8
7.2.1 Q uantitative productivity testing . 8
7.2.2 Q ualitative selectivity testing . 9
7.2.3 Qualitative specificity testing . 9
7.3 P reparation of a standardized test suspension using a working culture . 9
7.3.1 General . 9
7.3.2 Preparation of the working culture . 9
7.3.3 Preparation of a standardized test suspension (inoculum) for the test . 10
7.4 P reparation of a test suspension using reference material . 10
8 S ampling of membrane filters for testing .10
9 P rocedure .11
9.1 G eneral . 11
9.2 I noculation by spread plate technique . 11
9.2.1 G eneral . 11
9.2.2 Inoculation .12
9.3 I noculation by membrane filtration technique .12
9.3.1 General .12
9.3.2 Inoculation .12
9.4 I ncubation and counting . 13
9.5 T est for absence of microbial contamination . 13
10 C alculation, expression and interpretation of results .13
10.1 G eneral .13
10.2 P roductivity testing . 13
10.3 S electivity testing . 14
10.4 S pecificity testing .
...