EN ISO 16472:2006
(Main)Animal feeding stuffs - Determination of amylase-treated neutral detergent fibre content (aNDF) (ISO 16472:2006)
Animal feeding stuffs - Determination of amylase-treated neutral detergent fibre content (aNDF) (ISO 16472:2006)
ISO 16472:2006 specifies methods for the determination of amylase-treated neutral detergent insoluble fibrous residue content in all types of animal feed.
It includes a gravimetric routine method and a reference method.
Futtermittel - Bestimmung des amylase-behandelten neutral gereinigten Fasergehalts (ISO 16472:2006)
Diese Internationale Norm legt Verfahren zur Bestimmung des amylase-behandelten Faserrückstands, der in neutralem Waschmittel unlöslich ist, in allen Arten von Futtermitteln fest.
Sie beinhaltet ein gravimetrisches Routineverfahren und ein Referenzverfahren.
Aliments des animaux - Détermination du contenu en fibre détergente neutre traitée à l'amylase (ISO 16472:2006)
L'ISO 16472:2006 décrit une méthode de détermination du contenu en fibres résiduelles, insolubles, traitées à l'amylase et au détergent neutre, dans tous les types d'aliments pour animaux.
Elle inclut une méthode par gravimétrie courante et une méthode de référence.
Krma – Določevanje vlaknin z nevtralnim reagentom po predhodni obdelavi z amilazo (aNDF) (ISO 16472:2006)
General Information
Standards Content (Sample)
SLOVENSKI STANDARD
01-september-2006
.UPD±'RORþHYDQMHYODNQLQ]QHYWUDOQLPUHDJHQWRPSRSUHGKRGQLREGHODYL]
DPLOD]R D1') ,62
Animal feeding stuffs - Determination of amylase-treated neutral detergent fibre content
(aNDF) (ISO 16472:2006)
Futtermittel - Bestimmung des amylase-behandelten neutral gereinigten Fasergehalts
(ISO 16472:2006)
Aliments des animaux - Détermination du contenu en fibre détergente neutre traitée a
l'amylase (ISO 16472:2006)
Ta slovenski standard je istoveten z: EN ISO 16472:2006
ICS:
65.120 Krmila Animal feeding stuffs
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EUROPEAN STANDARD
EN ISO 16472
NORME EUROPÉENNE
EUROPÄISCHE NORM
April 2006
ICS 65.120
English Version
Animal feeding stuffs - Determination of amylase-treated neutral
detergent fibre content (aNDF) (ISO 16472:2006)
Aliments des animaux - Détermination du contenu en fibre Futtermittel - Bestimmung des amylase-behandelten
détergente neutre traitée à l'amylase (ISO 16472:2006) neutral gereinigten Fasergehalts (ISO 16472:2006)
This European Standard was approved by CEN on 23 March 2006.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Central Secretariat or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official
versions.
CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,
Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania,
Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
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© 2006 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 16472:2006: E
worldwide for CEN national Members.
Foreword
This document (EN ISO 16472:2006) has been prepared by Technical Committee ISO/TC 34
"Agricultural food products" in collaboration with Technical Committee CEN/TC 327 "Animal
feeding stuffs - Methods of sampling and analysis", the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of
an identical text or by endorsement, at the latest by October 2006, and conflicting national
standards shall be withdrawn at the latest by October 2006.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of
the following countries are bound to implement this European Standard: Austria, Belgium,
Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary,
Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
Endorsement notice
The text of ISO 16472:2006 has been approved by CEN as EN ISO 16472:2006 without any
modifications.
INTERNATIONAL ISO
STANDARD 16472
First edition
2006-04-15
Animal feeding stuffs — Determination of
amylase-treated neutral detergent fibre
content (aNDF)
Aliments des animaux — Détermination du contenu en fibre détergente
neutre traitée à l'amylase
Reference number
ISO 16472:2006(E)
©
ISO 2006
ISO 16472:2006(E)
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ii © ISO 2006 – All rights reserved
ISO 16472:2006(E)
Contents Page
Foreword. iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions. 1
4 Principle. 1
5 Reagents. 2
6 Apparatus . 2
7 Sampling. 3
8 Preparation of test sample. 3
9 Procedure . 4
9.1 Procedure for traditional method as described in Reference [1] . 4
9.2 Determination using Fibertec-type apparatus . 5
9.3 Modifications for specific types of samples . 7
9.4 Quality assurance. 8
10 Calculation and expression of results. 8
10.1 Calculation. 8
10.2 Expression of results . 9
11 Precision. 9
11.1 Interlaboratory test . 9
11.2 Repeatability. 9
11.3 Reproducibility. 10
12 Test report . 10
Annex A (informative) Results of interlaboratory test. 11
Annex B (informative) Standardization of heat-stable alpha-amylase working solution. 14
Bibliography . 16
ISO 16472:2006(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 16472 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 10, Animal
feeding stuffs.
iv © ISO 2006 – All rights reserved
INTERNATIONAL STANDARD ISO 16472:2006(E)
Animal feeding stuffs — Determination of amylase-treated
neutral detergent fibre content (aNDF)
WARNING — The use of this International Standard may involve the use of hazardous materials,
operations and equipment. This International Standard does not purport to address all the safety risks
associated with its use. It is the responsibility of the user of this International Standard to establish
appropriate safety and health practices and determine the applicability of local regulatory limitations
prior to use.
1 Scope
This International Standard specifies methods for the determination of amylase-treated neutral detergent
insoluble fibrous residue content in all types of animal feed.
It includes a gravimetric routine method and a reference method.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated
references, only the edition cited applies. For undated references, the latest edition of the referenced
document (including any amendments) applies.
ISO 6498, Animal feeding stuffs — Preparation of test samples
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
amylase-treated neutral detergent fibre content
aNDF content
mass fraction of insoluble fibre residues determined by the procedure specified in this International Standard
NOTE The aNDF content is expressed as a percentage by mass.
4 Principle
Neutral detergent (ND) solution and heat-stable alpha-amylase are used to dissolve the easily digestible
proteins, lipids, sugars, starches and pectins in feeds, leaving an insoluble fibrous residue that is primarily cell
wall components of plant materials (cellulose, hemicellulose and lignin) and indigestible nitrogenous matter in
animal products.
ISO 16472:2006(E)
5 Reagents
Use only reagents of recognized analytical grade, unless otherwise specified, and distilled or demineralized
water or water of equivalent purity.
5.1 Sodium sulfite, anhydrous (Na SO ).
2 3
5.2 Dried hominy corn (corn grits, raw), ground to pass through a 1 mm screen in a cutter mill.
5.3 Iodine solution, containing 2 g of potassium iodide and 1 g of iodine in 100 ml of water.
Store the solution in an amber or opaque bottle.
5.4 Heat-stable alpha-amylase, as a solution or a water extract of lyophilised enzyme powder (approx. 1 g
of powder extracted in 100 ml of water).
EXAMPLE Termamyl 120 l from Novo Enzymes or equivalent.
Standardize the heat-stable alpha-amylase solution or enzyme powder extract so that two additions of 2 ml
will remove starch from 0,5 g of raw corn starch (5.2). For a detailed procedure on standardizing heat-stable
alpha-amylase solution, see Annex B.
5.5 Neutral-detergent (ND) solution
Pour between 400 ml and 500 ml of water into a 1 l flask. Add 4,0 g of sodium hydroxide (NaOH) 14,6 g of
EDTA, 4,56 g of sodium hydrogen phosphate (Na HPO ), and 6,81 g of sodium borate decahydrate
2 4
⋅
(Na B O 10 H O) and mix until dissolved (heat if necessary). The NaOH and EDTA may be replaced with
2 4 7 2
18,6 g of disodium EDTA.
Under a safety hood, add 30 g of sodium lauryl sulfate and, after dissolution, add 10 ml of triethylene glycol
(anti-foaming aid). Add water to about 950 ml and mix. Adjust the pH to between 6,95 and 7,05 with
concentrated hydrochloric acid (HCl) or sodium hydroxide (NaOH) and dilute to 1 000 m
...
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