EN ISO 14673-1:2004

Milk and milk products - Determination of nitrate and nitrite contents - Part 1: Method using cadmium reduction and spectrometry (ISO 14673-1:2004)

ISO 14673-1|IDF 189-1:2004 specifies a method for the determination of the nitrate and nitrite contents of milk and milk products by cadmium reduction and spectrometry. The method is applicable to: whole and partly skimmed and skimmed dried milk; hard, semi-hard and soft cheeses; processed cheese; whey cheese, caseins and caseinates and dried whey.
The method may be performed using automatic equipment, in particular by segmented flow analysis (SFA) or flow injection analysis (FIA), thus reducing cadmium contamination in laboratory work places and waste water.

Milch und Milchprodukte - Bestimmung des Nitrat- und Nitritgehaltes - Teil 1: Verfahren mit Cadmiumreduktion und Spektrometrie (ISO 14673-1:2004)

Dieser Teil von ISO 14673|IDF 189 legt ein Verfahren zur Bestimmung des Nitrat- und Nitritgehaltes von Milch und Milchprodukten durch Cadmiumreduktion und Spektrometrie fest.
Das Verfahren gilt für
¾ vollständig und teilentrahmte Milch und Magermilchpulver;
¾ Hart-, Schnitt- und Weichkäse;
¾ Schmelzkäse;
¾ Molkenkäse, Caseine und Caseinate sowie Molkenpulver.
Das Verfahren kann unter Anwendung automatischer Geräte durchgeführt werden, besonders mit der segmentierten Fließanalyse (SFA) oder der Fließinjektionsanalyse (FIA), wodurch die Verunreinigung des Laborarbeitsplatzes und des Abwassers mit Cadmium verringert wird.
ANMERKUNG   Diese Verfahren sind in ISO 14673-2|IDF 189-2 bzw. in ISO 14673-3|IDF 189-3 beschrieben.

Lait et produits laitiers - Détermination des teneurs en nitrates et en nitrites - Partie 1: Méthode par réduction au cadmium et spectrométrie (ISO 14673-1:2004)

L'ISO 14673-1|FIL 189-1:2004 décrit une méthode de détermination des teneurs en nitrates et en nitrites dans les produits laitiers par réduction au cadmium et spectrométrie. La méthode s'applique: au lait entier, au lait demi-écrémé et au lait écrémé sec; aux fromages à pâte dure, semi-dure et aux fromages à pâte molle; aux fromages fondus; au lactosérum, aux caséines, aux caséinates et au lactosérum sec.
Cette méthode peut être réalisée à l'aide d'un appareil automatisé, en particulier par analyse de fluide segmentée (SFA) ou par analyse par injection (FIA), ce qui permet de réduire la contamination par le cadmium des laboratoires et des eaux usées.

Mleko in mlečni proizvodi - Določevanje nitratov in nitritov - 1. del: Metoda z uporabo redukcije s kadmijem in spektrometrijo (ISO 14673-1:2004)

General Information

• Status: Published
• Publication Date: 31-Jan-2004
• ICS: 67.100.01 - Milk and milk products in general
• Technical Committee: CEN/TC 302 - Milk and milk products - Methods of sampling and analysis
• Drafting Committee: CEN/TC 302 - Milk and milk products - Methods of sampling and analysis
• Current Stage: 9093 - Decision to confirm - Review Enquiry
• Start Date: 11-Jan-2013
• Completion Date: 11-Jan-2013
• Ref Project: SIST EN ISO 14673-1:2004 - Milk and milk products - Determination of nitrate and nitrite contents - Part 1: Method using cadmium reduction and spectrometry (ISO 14673-1:2004)

Relations

• Replaces: EN ISO 14673-1:2001 - Milk and milk products - Determination of nitrate and nitrite contents - Part 1: Method using cadmium reduction and spectrometry (ISO 14673-1:2001)
• Effective Date: 22-Dec-2008
• Replaces: EN ISO 14673-1:2001/AC:2002 - Milk and milk products - Determination of nitrate and nitrite contents - Part 1: Method using cadmium reduction and spectrometry (ISO 14673-1:2001)
• Effective Date: 22-Dec-2008

Standards Content (Sample)

SLOVENSKI STANDARD
01-maj-2004
0OHNRLQPOHþQLSURL]YRGL'RORþHYDQMHQLWUDWRYLQQLWULWRYGHO0HWRGD]
XSRUDERUHGXNFLMHVNDGPLMHPLQVSHNWURPHWULMR,62
Milk and milk products - Determination of nitrate and nitrite contents - Part 1: Method
using cadmium reduction and spectrometry (ISO 14673-1:2004)
Milch und Milchprodukte - Bestimmung des Nitrat- und Nitritgehaltes - Teil 1: Verfahren
mit Cadmiumreduktion und Spektrometrie (ISO 14673-1:2004)
Lait et produits laitiers - Détermination des teneurs en nitrates et en nitrites - Partie 1:
Méthode par réduction au cadmium et spectrométrie (ISO 14673-1:2004)
Ta slovenski standard je istoveten z: EN ISO 14673-1:2004
ICS:
67.100.01 0OHNRLQPOHþQLSURL]YRGLQD Milk and milk products in
VSORãQR general
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 14673-1
NORME EUROPÉENNE
EUROPÄISCHE NORM
February 2004
ICS 67.100.10 Supersedes EN ISO 14673-1: 2001
English version
Milk and milk products - Determination of nitrate and nitrite
contents - Part 1: Method using cadmium reduction and
spectrometry (ISO 14673-1:2004)
Lait et produits laitiers - Détermination des teneurs en Milch und Milchprodukte - Bestimmung des Nitrat- und
nitrates et en nitrites - Partie 1: Méthode par réduction au Nitritgehaltes - Teil 1: Verfahren mit Cadmiumreduktion
cadmium et spectrométrie (ISO 14673-1:2004) und Spektrometrie (ISO 14673-1:2004)
This European Standard was approved by CEN on 2 February 2004.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Central Secretariat or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official
versions.
CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,
Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia,
Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2004 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 14673-1:2004: E
worldwide for CEN national Members.

CORRECTED  2004-02-25
Foreword
This document (EN ISO 14673-1:2004) has been prepared by Technical Committee ISO/TC 34
"Agricultural food products" in collaboration with Technical Committee CEN/TC 302 "Milk and
milk products - Methods of sampling and analysis", the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of
an identical text or by endorsement, at the latest by August 2004, and conflicting national
standards shall be withdrawn at the latest by August 2004.
This document supersedes EN ISO 14673-1:2001.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of
the following countries are bound to implement this European Standard: Austria, Belgium,
Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary,
Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
Portugal, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
Endorsement notice
The text of ISO 14673-1:2004 has been approved by CEN as EN ISO 14673-1:2004 without any
modifications.
NOTE Normative references to International Standards are listed in Annex ZA (normative).
Annex ZA
(normative)
Normative references to international publications
with their relevant European publications
This European Standard incorporates by dated or undated reference, provisions from other
publications. These normative references are cited at the appropriate places in the text and the
publications are listed hereafter. For dated references, subsequent amendments to or revisions of
any of these publications apply to this European Standard only when incorporated in it by
amendment or revision. For undated references the latest edition of the publication referred to
applies (including amendments).
NOTE Where an International Publication has been modified by common modifications, indicated
by (mod.), the relevant EN/HD applies.
Publication Year Title EN Year
ISO 1042 1998 Laboratory glassware - One- EN ISO 1042 1999
mark volumetric flasks
INTERNATIONAL ISO
STANDARD 14673-1
IDF
189-1
Second edition
2004-02-01
Milk and milk products — Determination
of nitrate and nitrite contents —
Part 1:
Method using cadmium reduction and
spectrometry
Lait et produits laitiers — Détermination des teneurs en nitrates et en
nitrites —
Partie 1: Méthode par réduction au cadmium et spectrométrie

Reference numbers
ISO 14673-1:2004(E)
IDF 189-1:2004(E)
©
ISO and IDF 2004
ISO 14673-1:2004(E)
IDF 189-1:2004(E)
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ISO 14673-1:2004(E)
IDF 189-1:2004(E)
Contents Page
Foreword. iv
1 Scope. 1
2 Normative references . 1
3 Terms and definitions. 2
4 Principle . 2
5 Reagents . 2
6 Apparatus. 4
7 Sampling . 5
8 Preparation of test sample. 5
8.1 Dried milk and dried whey. 5
8.2 Caseins and caseinates. 5
8.3 Cheese. 6
8.4 Whey cheese . 6
9 Procedure. 6
9.1 Preparation of the copperized cadmium column . 6
9.2 Checking the reducing capacity of the column . 6
9.3 Regeneration of the column. 7
9.4 Preparation of test portion. 7
9.5 Extraction and deproteination . 8
9.6 Reduction of nitrate to nitrite. 8
9.7 Blank test . 9
9.8 Determination . 9
9.9 Preparation of calibration graph . 9
10 Calculation and expression of results . 9
10.1 Nitrite content. 9
10.2 Nitrate content. 10
11 Precision . 10
11.1 General. 10
11.2 Repeatability. 10
12 Test report. 11
Bibliography . 13

ISO 14673-1:2004(E)
IDF 189-1:2004(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
ISO technical committees. Each member body interested in a subject for which a technical committee has
been established has the right to be represented on that committee. International organizations, governmental
and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 14673-1IDF 189-1 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee
SC 5, Milk and milk products, and the International Dairy Federation (IDF), in collaboration with AOAC
International. It is being published jointly by ISO and IDF and separately by AOAC International.
This second edition cancels and replaces the first edition (ISO 14673-1IDF 189-1:2001), of which it
constitutes a minor revision.
ISO 14673IDF 189 consists of the following parts, under the general title Milk and milk products —
Determination of nitrate and nitrite contents:
— Part 1: Method using cadmium reduction and spectrometry
— Part 2: Method using segmented flow analysis (Routine method)
— Part 3: Method using cadmium reduction and flow injection analysis with in-line dialysis (Routine method)
iv © ISO and IDF 2004 – All
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