prEN 18034

Algae and algae products - Methods of sampling and analysis - Determination of chlorophyll a content

This document specifies a laboratory method for the determination of chlorophyll a content in algae. The method was initially tested and evaluated on the microalgae species Nannochloropsis and a heat treated algal product tomato soup with Nannochloropsis supplement, and the macro algae species Ulva sp, Furcellaria lumbricalis, and Saccharina latissima. During an Interlaboratory Trial the method was tested on the microalgae species Nannochloropsis and the macro algae species Saccharina latissima. The microalgae species Nannochloropsis and Phaodactlylum and the macro algae species Ulva sp and Saccharina latissima were tested in a Round Robin test. This document is only validated for chlorophyll a, but it can be used for other chlorophylls as well.
If possible, an attempt will be made to incorporate an alternative method than the HPLC method proposed into the standard (informative or normative), on the condition that research on the use of a cheaper method fits into the budget allocated for this topic. The more, it is imperative that this additional method should obtain comparable results and accuracy as the HPLC method.
The schedules of all the additional experiments mentioned are based on the schedule of the standard on chlorophyll.

Algen und algenbasierte Produkte oder Zwischenprodukte - Verfahren zur Probenentnahme und Analyse - Quantifizierung von Chlorophyll

Dieses Dokument legt ein Laborverfahren zur Quantifizierung von Chlorophyll a in Algen fest. Das Verfahren wurde ursprünglich an der Mikroalgenart Nannochloropsis und einer wärmebehandelten Algenprodukt-Tomatensuppe mit Nannochloropsis-Zusatz und an den Makroalgenarten Ulva sp, Furcellaria lumbricalis und Saccharina latissima geprüft und evaluiert. Während eines Ringversuchs wurde das Verfahren an der Mikroalgenart Nannochloropsis und der Makroalgenart Saccharina latissima untersucht. Die Mikroalgenarten Nannochloropsis und Phaeodactylum und die Makroalgenarten Ulva sp und Saccharina latissima wurden in einem Ringvergleich untersucht. Dieses Dokument ist nur für Chlorophyll a validiert, aber es kann auch für andere Chlorophyllarten verwendet werden.

Algues et produits ou intermédiaires à base d'algues - Méthodes d'échantillonnage et d'analyse - Détermination de la teneur en chlorophylle a

Le présent document spécifie une méthode de détermination en laboratoire de la teneur en chlorophylle a des algues. La méthode a été initialement soumise à essai et évaluée sur la microalgue Nannochloropsis et sur une soupe à la tomate à base de produit d’algue traité thermiquement contenant le complément Nannochloropsis et les macroalgues Ulva, Furcellaria lumbricalis et Saccharina latissima. Lors d’un essai interlaboratoires, la méthode a été soumise à essai sur la microalgue Nannochloropsis et la macroalgue Saccharina latissima. Les microalgues Nannochloropsis et Phaeodactylum et les macroalgues Ulva et Saccharina latissima ont été évaluées au cours d’un essai comparatif. Le présent document n’est validé que pour la chlorophylle a, mais il peut également être utilisé pour d’autres chlorophylles.

Alge in izdelki iz alg - Metode vzorčenja in analize - Določevanje vsebnosti klorofila

General Information

• Status: Not Published
• Publication Date: 02-Jun-2022
• ICS: 13.020.55 - Biobased products
• Technical Committee: CEN/TC 454 - Algae and algae products
• Drafting Committee: CEN/TC 454/WG 6 - Product test methods
• Current Stage: 4060 - Closure of enquiry - Enquiry
• Start Date: 08-Feb-2024
• Due Date: 28-Jan-2021
• Completion Date: 08-Feb-2024
• Mandate: M/547 - [C(2016)1582] Standardisation request to CEN on Algae and their products
• Ref Project: oSIST prEN 18034:2024 - Algae and algae products - Methods of sampling and analysis - Determination of chlorophyll a content

Standards Content (Sample)

SLOVENSKI STANDARD
oSIST prEN 18034:2024
01-januar-2024
Alge in izdelki iz alg - Metode vzorčenja in analize - Določevanje vsebnosti klorofila
Algae and algae products - Methods of sampling and analysis - Determination of
chlorophyll a content
Algen und algenbasierte Produkte oder Zwischenprodukte - Verfahren zur
Probenentnahme und Analyse - Quantifizierung von Chlorophyll
Algues et produits ou intermédiaires à base d'algues - Méthodes d'échantillonnage et
d'analyse - Détermination de la teneur en chlorophylle a
Ta slovenski standard je istoveten z: prEN 18034
ICS:
13.020.55 Biološki izdelki Biobased products
oSIST prEN 18034:2024 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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oSIST prEN 18034:2024

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oSIST prEN 18034:2024


DRAFT
EUROPEAN STANDARD
prEN 18034
NORME EUROPÉENNE

EUROPÄISCHE NORM

November 2023
ICS
English Version

Algae and algae products or intermediates - Methods of
sampling and analysis - Determination of chlorophyll a
content
Algues et produits à base d'algues - Méthodes Algen und Algenprodukte - Methoden zur
d'échantillonnage et d'analyse - Détermination de la Probeentnahme und Analyse - Bestimmung von
teneur en chlorophylle a Chlorophyll a gehalts
This draft European Standard is submitted to CEN members for enquiry. It has been drawn up by the Technical Committee
CEN/TC 454.

If this draft becomes a European Standard, CEN members are bound to comply with the CEN/CENELEC Internal Regulations
which stipulate the conditions for giving this European Standard the status of a national standard without any alteration.

This draft European Standard was established by CEN in three official versions (English, French, German). A version in any other
language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC
Management Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.

Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.

Warning : This document is not a European Standard. It is distributed for review and comments. It is subject to change without
notice and shall not be referred to as a European Standard.


EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATIO N

EUROPÄISCHES KOMITEE FÜR NORMUN G

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2023 CEN All rights of exploitation in any form and by any means reserved Ref. No. prEN 18034:2023 E
worldwide for CEN national Members.

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prEN 18034:2023 (E)
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 6
5 Apparatus . 6
6 Reagents and materials . 6
7 Sampling and preparation of the sample . 7
8 Procedure . 8
9 Calculation of ch
...