EN ISO 8420:1995

Animal and vegetable fats and oils - Determination of polar compounds content (ISO 8420:1990)

Tierische und pflanzliche Fette und Öle - Bestimmung des Gehalts an polaren Bestandteilen (ISO 8420:1990)

Diese Internationale Norm beschreibt ein Verfahren zur Bestimmung des Gehalts an polaren Bestandteilen in tierischen und pflanzlichen Fetten und Ölen, die nachfolgend als Fette bezeichnet werden. Anmerkung 1: Da beim Erhitzen von Fetten polare Verbindungen entstehen, dient das Verfahren zur Beurteilung des Belastungßustandes gebrauchter Fritierfette.

Corps gras d'origines animale et végétale - Dosage des composés polaires (ISO 8420:1990)

La CEI 60748-2-20:2008 présente norme donne des spécifications d'interface en courant continu pour différentes séries de valeurs comprenant chacune la valeur nominale de la tension d'alimentation, sa tolérance et les valeurs limites en pire cas des tensions d'entrée et de sortie pour les circuits intégrés à basse tension.
Cette deuxième édition annule et remplace la première édition parue en 2000. Cette édition constitue une révision technique.
Cette édition inclut les modifications techniques majeures suivantes par rapport à l'édition précédente:
- Extension de la limite inférieure de l'alimentation de puissance à 1,0 V nominal
- Ajout des spécifications d'entrée "trigger de Schmitt"

Živalske in rastlinske maščobe in olja - Določevanje polarnih spojin (ISO 8420:1990)

General Information

• Status: Withdrawn
• Publication Date: 27-Apr-1995
• Withdrawal Date: 31-Mar-2002
• ICS: 67.200.10 - Animal and vegetable fats and oils
• Technical Committee: CEN/TC 307 - Oilseeds, vegetables and animal fats and oils and their by-products - Methods of sampling and analys
• Drafting Committee: CEN/TC 307 - Oilseeds, vegetables and animal fats and oils and their by-products - Methods of sampling and analys
• Current Stage: 9960 - Withdrawal effective - Withdrawal
• Start Date: 01-Apr-2002
• Completion Date: 01-Apr-2002
• Ref Project: SIST EN ISO 8420:1998 - Animal and vegetable fats and oils - Determination of polar compounds content (ISO 8420:1990)

Relations

• Replaced By: EN ISO 8420:2002 - Animal and vegetable fats and oils - Determination of content of polar compounds (ISO 8420:2002)
• Effective Date: 22-Dec-2008

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 8420:1998
01-november-1998
äLYDOVNHLQUDVWOLQVNHPDãþREHLQROMD'RORþHYDQMHSRODUQLKVSRMLQ,62

Animal and vegetable fats and oils - Determination of polar compounds content (ISO
8420:1990)
Tierische und pflanzliche Fette und Öle - Bestimmung des Gehalts an polaren
Bestandteilen (ISO 8420:1990)
Corps gras d'origines animale et végétale - Dosage des composés polaires (ISO
8420:1990)
Ta slovenski standard je istoveten z: EN ISO 8420:1995
ICS:
67.200.10 5DVWOLQVNHLQåLYDOVNH Animal and vegetable fats
PDãþREHLQROMD and oils
SIST EN ISO 8420:1998 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 8420:1998

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SIST EN ISO 8420:1998

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SIST EN ISO 8420:1998

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SIST EN ISO 8420:1998

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SIST EN ISO 8420:1998

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SIST EN ISO 8420:1998
INTERNATION
STANDARD
8420
First edition
1990-l l-01
Animal and vegetable fats and oils -
Determination of polar compounds content
Corps gra s d’origines animale et v6geiale - Dosage des cornposh
polaires
--- -------
Reference number
IS0 8420: 1990(E)

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SIST EN ISO 8420:1998
IS0 8420:1990(E)
Foreword
IS0 (the International Organization for Standardization) is a worldwide
federation of national standards bodies (IS0 member bodies). The work
of preparing International Standards is normally carried out through IS0
technical committees. Each member body interested in a subject for
which a technical committee has been established has the right to be
represented on that committee. International organizations, govern-
mental and non-governmental, in liaison with ISO, also take part in the
work. IS0 collaborates closely with the International Electrotechnical
Commission (IEC) on all matters of electrotechnical standardization.
Draft International Standards adopted by the technical committees are
circulated to the member bodies for voting. Publication as an lnter-
national Standard requires approval by at least 75 % of the member
bodies casting a vote.
International Standard IS0 8420 was prepared by Technical Committee
ISO/TC 34, Agricultural food products.
Annex A of this International Standard is for information only.
0 IS0 1990
Ail rights reserved. No part of this publication may be reproduced or utilized in any form
or by any means, electronic or mechanical, including photocopying and microfilm, without
permission in writing from the publisher.
International Organization for Standardization
Case Postale 56 l CH-1211 Get-&/e 20 l Switzerland
Printed in Switzerland
ii

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SIST EN ISO 8420:1998
Animal and vegetable fats and oils - Determination of polar
compounds content
Polar compounds include polar substances which
1 Scope
occur in unused fats, such as monoglycerides,
diqlycerides and free fatty acids, as well as polar
This International Standard describes a method for
transformation products formed during heating as
the determination of the content of polar compounds
occurs during the frying of food. Non-polar com-
in animal and vegetable fats and oils, hereinafter
pounds are mostly unaltered triglycerides.
referred to as fats.
NOTE 1 Polar compounds are formed during the heating
of fats and thus the method serves to assess the deteri-
4 Principle
oration of frying fats with use.
Separation of a test portion by column chro-
matography into non-polar and polar compounds.
2 Normative references
Elution of the non-polar compounds and weighing
of them. Determination of the polar compounds by
The following standards contain provisions which,
difference.
through reference in this text, constitute provisions
of this International Standard. At the time of publi-
cation, the editions indicated were valid. All stan-
dards are subject to revision, and parties to
5 Reagents and materials
agreements based on this International Standard
are encouraged to investigate the possibility of ap-
All reagents shall be of recognized analytical grade
plying the most recent editions of the standards in-
and the water used shall be distilled water or water
dicated below. Members of IEC and ISO maintain
of equivalent purity.
registers of currently valid International Standards.
IS0 661 :I 989, Animal and vegetable fats and oils -
5.1 Silica gel, of particle size 0,063 mm to
Preparaliorl of test sample.
0,200 mm (70 mesh to 230 mesh), such as Merck
No. 7734*), adjusted to a water content of 5 % (H~/RZ)
IS0 5555:~-1, , Animal and vegetable fats and oils -
as follows.
Sampling.
Place a shallow layer of the silica gel in a porcelain
dish, dry in an oven at 155 OC to 160 OC for at least
3 Definition 4 h with occasional stirring and cool in a desiccator
to room temperature. Adjust the water content of the
For the purposes of this International Standard, the silica gel to 5 % (m/nz) by placing 152 g of silica gel
following definition applies. and 8 g of water in a 500 ml flask. Stopper the flask
and shake on a shaking machine for 20 min. Deter-
polar compounds: Constituents of fats which are mine the water content by drying at 155 “C to 160 OC
if necessary, adjust to 5 % ( m/m) -t
determined by column chromatography under the and,
-
conditions specified in this International Standard. 0,2 % (m/m).
1) To be published. (Revision of IS0 5555:1983)
2) Merck No. 7734 is the trade-name of a product supplied by Merck. This information is given for the convenience of users
of this International Standard and does not constitute an endorsement by IS0 of the product named. Equivalent products
may be used if they can be shown to lead to the same results.

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SIST EN ISO 8420:1998
IS0 8420:1990(E)
and pour this slurry into the column using a funnel.
Store the silica gel in a tightly closed container, If
Complete the transfer of the silica qet into the col-
not used within 24 h, check the moisture content and
adjust if necessary. umn by rinsing the beaker with the etution solvent.
Open the stopcock and run off the elution soivent
5.2 Elution solvent, prepared by mixing 87 volumes
until the level of the elution solvent is about 100 mm
of chromatographic quality light petroleum (boiling
above the silica get. Level the silica gel by tapping
range, 40 OC to 60 T) and 13 volumes of stabilized
the column.
diethyl ether (see warning to 9.5.4).
Add about 4 g of the sand (5.3) through the funnel.
5.3 Sand, acid washed and calcined. Run off the supernatant elution solvent to within
IO mm of the sand layer.
5.4 Cotton wool, surgical quality, non-absorbent.
Discard the elution solvent used in the preparation
of the column.
5.5 Nitrogen, 99,0 % to 99,8 % purity.
9.2 Blank test
6 Apparatus
Pass I50 ml of the etution solvent through the col-
Usual laboratory apparatus and, in particular, the
umn. Collect the solvent in a 250 ml flask (6.1)
foI low ing.
weighed to the nearest 1 mg, evaporat.e the solvent
as described in 9.5.4 and weigh again to the nearest
6.1 Round- or flat-bottom flasks, with a ground
1 mg. Calculate the mass, in grams, of the blank by
neck, of 250 ml capacity.
subtraction.
6.2 Chromatographic column, made of glass, of
9.3 Assessment of column efficiency
21 mm internal diameter and 450 mm in length,
equipped with a stopcock (preferably made of poly-
If desired, assess the column efficiency in a
...