Plant biostimulants - Determination of the yeast and mould content

This document specifies a horizontal method for the enumeration of yeasts and moulds present in plant biostimulants intended for use in agriculture, by means of the colony count technique after aerobic incubation at 25 °C ± 2,5 °C.
This document allows the enumeration of yeasts and moulds, in technical and formulated plant biostimulant, both in liquid and solid state. The method is applicable to microbial plant biostimulant except those composed of fungi or yeast to verify that the concentration of yeast and moulds does not exceed the respective limits described in the EU Fertilisers Regulation [1].
If necessary, yeast and mould enumerated can be identified using suitable identification tests.

Pflanzen-Biostimulanzien - Bestimmung des Gehalts an Hefen und Schimmelpilzen

Dieses Dokument legt ein horizontales Verfahren zur Zählung von Hefen und Schimmelpilzen in Pflanzen-Biostimulanzien, die zur Verwendung in der Landwirtschaft bestimmt sind, mit Hilfe des Koloniezählverfahrens nach aerober Bebrütung bei (25 ± 2,5) °C fest.
Dieses Dokument hat die Zählung von Hefen und Schimmelpilzen in technischen und formulierten Pflanzen-Biostimulanzien, die im flüssigen oder im festen Zustand vorliegen können, zum Gegenstand. Das Verfahren ist auf mikrobielle Pflanzen-Biostimulanzien anwendbar, ausgenommen jene, die aus Pilzen oder Hefe bestehen, um zu überprüfen, dass die Konzentration von Hefe und Schimmelpilzen die jeweiligen Grenzwerte, die in der EU-Düngeprodukteverordnung [1] angegeben sind, nicht überschreitet.
Gegebenenfalls können die gezählten Hefen und Schimmelpilze durch geeignete Identifizierungsprüfungen identifiziert werden.

Biostimulants des végétaux - Détermination de la teneur en levures et en moisissures

Le présent document spécifie une méthode horizontale pour le dénombrement des levures et des moisissures présentes dans les biostimulants des végétaux destinés à être utilisés en agriculture, par la technique de comptage des colonies après incubation aérobie à 25 °C ± 2,5 °C.
Le présent document permet le dénombrement des levures et des moisissures, dans les biostimulants des végétaux de qualité technique, à la fois à l’état liquide et à l’état solide. La méthode est applicable aux biostimulants microbiens des végétaux, excepté ceux composés de champignons ou de levures, pour vérifier que la concentration des levures et des moisissures ne dépasse pas les limites correspondantes indiquées dans le Règlement UE sur les fertilisants [1].
Si nécessaire, les levures et les moisissures dénombrées peuvent être identifiées par des essais d’identification appropriés.

Rastlinski biostimulanti - Določanje kvasovk in plesni

Ta dokument določa vodoravno metodo za ugotavljanje števila kvasovk in plesni, prisotnih v rastlinskem biostimulantu, namenjenem za uporabo v kmetijstvu, s tehniko štetja kolonij po aerobni inkubaciji pri 25 °C ± 2,5 °C.
Ta dokument dovoljuje štetje kvasovk in plesni v tehničnih ter formuliranih rastlinskih biostimulantih tako v tekočem kot v trdnem stanju. Metoda se uporablja za mikrobne rastlinske biostimulante, razen tistih, ki so sestavljeni iz gliv ali kvasovk za preverjanje, da koncentracija kvasovk in plesni ne presega ustreznih mejnih vrednosti, opisanih v Uredbi EU o sredstvih za gnojenje [1].
Po potrebi je mogoče preštete kvasovke in plesni identificirati z ustreznimi identifikacijskimi preskusi.

General Information

Status
Published
Publication Date
22-Mar-2022
Current Stage
6060 - Definitive text made available (DAV) - Publishing
Start Date
23-Mar-2022
Due Date
29-Aug-2022
Completion Date
23-Mar-2022

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Standards Content (Sample)

SLOVENSKI STANDARD
SIST-TS CEN/TS 17707:2023
01-februar-2023
Rastlinski biostimulanti - Določanje kvasovk in plesni
Plant biostimulants - Determination of the yeast and mould content
Pflanzen-Biostimulanzien - Bestimmung des Gehalts an Hefen und Schimmelpilzen
Biostimulants des végétaux - Détermination de la teneur en levures et en moisissures
Ta slovenski standard je istoveten z: CEN/TS 17707:2022
ICS:
65.080 Gnojila Fertilizers
SIST-TS CEN/TS 17707:2023 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST-TS CEN/TS 17707:2023

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SIST-TS CEN/TS 17707:2023


CEN/TS 17707
TECHNICAL SPECIFICATION

SPÉCIFICATION TECHNIQUE

March 2022
TECHNISCHE SPEZIFIKATION
ICS 65.080
English Version

Plant biostimulants - Determination of the yeast and
mould content
Biostimulants des végétaux - Détermination de la Biostimulanzien für die pflanzliche Anwendung -
teneur en levures et en moisissures Bestimmung des Gehalts an Hefen und Schimmelpilzen
This Technical Specification (CEN/TS) was approved by CEN on 3 January 2022 for provisional application.
This Technical Specification was corrected and reissued by the CEN-CENELEC Management Centre on 27 April 2022.

The period of validity of this CEN/TS is limited initially to three years. After two years the members of CEN will be requested to
submit their comments, particularly on the question whether the CEN/TS can be converted into a European Standard.

CEN members are required to announce the existence of this CEN/TS in the same way as for an EN and to make the CEN/TS
available promptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in
parallel to the CEN/TS) until the final decision about the possible conversion of the CEN/TS into an EN is reached.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.



EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATIO N

EUROPÄISCHES KOMITEE FÜR NORMUN G

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2022 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN/TS 17707:2022 E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------
SIST-TS CEN/TS 17707:2023
CEN/TS 17707:2022 (E)
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 6
2 Normative references . 6
3 Terms and definitions . 6
4 Principles . 7
4.1 General . 7
4.2 Plate count method . 7
5 Diluent and culture media . 7
5.1 Diluent . 7
5.2 Culture media . 7
6 Apparatus and glassware . 7
7 Handling of plant biostimulants and sampling . 8
8 Procedure . 8
8.1 General .
...

SLOVENSKI STANDARD
kSIST-TS FprCEN/TS 17707:2021
01-november-2021
Rastlinski biostimulansi - Določevanje kvasovk in plesni
Plant biostimulants - Determination of the yeast and mould content
Biostimulanzien für die pflanzliche Anwendung - Bestimmung des Gehalts an Hefen und
Schimmelpilzen
Ta slovenski standard je istoveten z: FprCEN/TS 17707
ICS:
65.080 Gnojila Fertilizers
kSIST-TS FprCEN/TS 17707:2021 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
kSIST-TS FprCEN/TS 17707:2021

---------------------- Page: 2 ----------------------
kSIST-TS FprCEN/TS 17707:2021


FINAL DRAFT
TECHNICAL SPECIFICATION
FprCEN/TS 17707
SPÉCIFICATION TECHNIQUE

TECHNISCHE SPEZIFIKATION

September 2021
ICS 65.080
English Version

Plant biostimulants - Determination of the yeast and
mould content
 Biostimulanzien für die pflanzliche Anwendung -
Bestimmung des Gehalts an Hefen und Schimmelpilzen


This draft Technical Specification is submitted to CEN members for Vote. It has been drawn up by the Technical Committee
CEN/TC 455.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.

Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.

Warning : This document is not a Technical Specification. It is distributed for review and comments. It is subject to change
without notice and shall not be referred to as a Technical Specification.


EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2021 CEN All rights of exploitation in any form and by any means reserved Ref. No. FprCEN/TS 17707:2021 E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------
kSIST-TS FprCEN/TS 17707:2021
FprCEN/TS 17707:2021 (E)
Contents Page
European foreword . 4
Introduction . 5
1 Scope . 7
2 Normative references . 7
3 Terms and definitions . 7
4 Principles . 8
4.1 General . 8
4.2 Plate count method . 8
5 Diluent and culture media . 8
5.1 Diluent . 9
5.2 Culture media . 9
6 Apparatus and glassware . 9
7 Handling of plant biostimulants and sampling . 9
8 Procedure . 9
8.1 General . 9
8.2 Test portion and initial suspension . 9
8.2.1 General . 9
8.2.2 Liquid - water based - formulations . 9
8.2.3 Liquid – oil based (emulsi
...

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