ISO 15914-2:2024

Animal feeding stuffs — Enzymatic determination of total starch content — Part 2: Method by enzymatic determination with a hexokinase system and potassium hydroxide dispersion

This document specifies an enzymatic method for determining starch in animal feeding stuffs containing starchy ingredients (cereals, tubers, etc.). The method is also applicable to beans and to the animal digestive contents because it involves the hexokinase system for the final glucose determination.

Alimentation animale — Dosage enzymatique de la teneur totale en amidon — Partie 2: Méthode par dosage enzymatique avec un système hexokinase et dispersion à l’hydroxyde de potassium

Le présent document spécifie une méthode enzymatique de dosage de l’amidon dans l’alimentation animale contenant des ingrédients amylacés (céréales, tubercules, etc.). La méthode s’applique également aux fèves et aux contenus digestifs des animaux, car elle fait intervenir le système hexokinase pour le dosage final du glucose.

General Information

• Status: Published
• Publication Date: 23-May-2024
• ICS: 65.120 - Animal feeding stuffs
• Technical Committee: ISO/TC 34/SC 10 - Animal feeding stuffs
• Drafting Committee: ISO/TC 34/SC 10 - Animal feeding stuffs
• Current Stage: 6060 - International Standard published
• Start Date: 24-May-2024
• Due Date: 16-Sep-2025
• Completion Date: 24-May-2024

Standards Content (Sample)

International
Standard
ISO 15914-2
First edition
Animal feeding stuffs — Enzymatic
2024-05
determination of total starch
content —
Part 2:
Method by enzymatic determination
with a hexokinase system and
potassium hydroxide dispersion
Alimentation animale — Dosage enzymatique de la teneur totale
en amidon —
Partie 2: Méthode par dosage enzymatique avec un système
hexokinase et dispersion à l’hydroxyde de potassium
Reference number
ISO 15914-2:2024(en) © ISO 2024

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ISO 15914-2:2024(en)
COPYRIGHT PROTECTED DOCUMENT
© ISO 2024
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: [phone]
Email: [e-mail]
Website: www.iso.org
Published in Switzerland

© ISO 2024 – All rights reserved
ii

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ISO 15914-2:2024(en)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 1
6 Equipment . 2
7 Sampling . 3
8 Preparation of the sample for testing . 3
9 Operating procedure . 3
9.1 Test sample .3
9.2 Washing, dispersion and hydrolysis .3
9.3 Determination of glucose .3
9.4 Control of the efficiency of the enzyme .4
10 Expression of the results . 4
11 Precision . 5
11.1 Interlaboratory test . .
...

Norme
internationale
ISO 15914-2
Première édition
Alimentation animale — Dosage
2024-05
enzymatique de la teneur totale en
amidon —
Partie 2:
Méthode par dosage enzymatique
avec un système hexokinase
et dispersion à l’hydroxyde de
potassium
Animal feeding stuffs — Enzymatic determination of total starch
content —
Part 2: Method by enzymatic determination with a hexokinase
system and potassium hydroxide dispersion
Numéro de référence
ISO 15914-2:2024(fr) © ISO 2024

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ISO 15914-2:2024(fr)
DOCUMENT PROTÉGÉ PAR COPYRIGHT
© ISO 2024
Tous droits réservés. Sauf prescription différente ou nécessité dans le contexte de sa mise en œuvre, aucune partie de cette
publication ne peut être reproduite ni utilisée sous quelque forme que ce soit et par aucun procédé, électronique ou mécanique,
y compris la photocopie, ou la diffusion sur l’internet ou sur un intranet, sans autorisation écrite préalable. Une autorisation peut
être demandée à l’ISO à l’adresse ci-après ou au comité membre de l’ISO dans le pays du demandeur.
ISO copyright office
Case postale 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Genève
Tél.: [phone]
E-mail: [e-mail]
Web: www.iso.org
Publié en Suisse

© ISO 2024 – Tous droits réservés
ii

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ISO 15914-2:2024(fr)
Sommaire Page
Avant-propos .iv
1 Domaine d’application . 1
2 Références normatives . 1
3 Termes et définitions . 1
4 Principe. 1
5 Réactifs . 1
6 Équipement . 2
7 Échantillonnage . 3
8 Préparation de l’échantillon en vue des essais. 3
9 Mode opératoire . 3
9.1 Échantillon pour essai .3
9.2 Lavage, dispersion et hydrolyse .3
9.3 Dosage du glucose .4
9.4 Contrôle de l’efficacité de l’enzyme .4
10 Expression des résultats . 5
11 Fidélité . 5
11.1 Essai interlaboratoires .
...

FINAL DRAFT
International
Standard
ISO/FDIS 15914-2
ISO/TC 34/SC 10
Animal feeding stuffs — Enzymatic
Secretariat: INSO
determination of total starch
Voting begins on:
content —
2024-03-05
Part 2:
Voting terminates on:
2024-04-30
Method by enzymatic determination
with a hexokinase system and
potassium hydroxide dispersion
Alimentation animale — Détermination enzymatique de la
teneur totale en amidon —
Partie 2: Méthode par dosage enzymatique avec un système
hexokinase et dispersion à l’hydroxyde de potassium
RECIPIENTS OF THIS DRAFT ARE INVITED TO SUBMIT,
WITH THEIR COMMENTS, NOTIFICATION OF ANY
RELEVANT PATENT RIGHTS OF WHICH THEY ARE AWARE
AND TO PROVIDE SUPPOR TING DOCUMENTATION.
IN ADDITION TO THEIR EVALUATION AS
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO-
LOGICAL, COMMERCIAL AND USER PURPOSES, DRAFT
INTERNATIONAL STANDARDS MAY ON OCCASION HAVE
TO BE CONSIDERED IN THE LIGHT OF THEIR POTENTIAL
TO BECOME STAN DARDS TO WHICH REFERENCE MAY BE
MADE IN NATIONAL REGULATIONS.
Reference number
ISO/FDIS 15914-2:2024(en) © ISO 2024

---------------------- Page: 1 ----------------------
FINAL DRAFT
ISO/FDIS 15914-2:2024(en)
International
Standard
ISO/FDIS 15914-2
ISO/TC 34/SC 10
Animal feeding stuffs — Enzymatic
Secretariat: INSO
determination of total starch
Voting begins on:
content —
Part 2:
Voting terminates on:
Method by enzymatic determination
with a hexokinase system and
potassium hydroxide dispersion
Alimentation animale — Détermination enzymatique de la
teneur totale en amidon —
Partie 2: Méthode par dosage enzymatique avec un système
hexokinase et dispersion à l’hydroxyde de potassium
RECIPIENTS OF THIS DRAFT ARE INVITED TO SUBMIT,
COPYRIGHT PROTECTED DOCUMENT
WITH THEIR COMMENTS, NOTIFICATION OF ANY
RELEVANT PATENT RIGHTS OF WHICH THEY ARE AWARE
AND TO PROVIDE SUPPOR TING DOCUMENTATION.
© ISO 2024
IN ADDITION TO THEIR EVALUATION AS
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO-
LOGICAL, COMMERCIAL AND USER PURPOSES, DRAFT
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
INTERNATIONAL STANDARDS MAY ON OCCASION HAVE
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
TO BE CONSIDERED IN THE LIGHT OF THEIR POTENTIAL
or ISO’s member body in the country of the requester.
TO BECOME STAN DARDS TO WHICH REFERENCE MAY BE
MADE IN NATIONAL REGULATIONS.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: [phone]
Email: [e-mail]
Website: www.iso.org
Published in Switzerland Reference number
ISO/FDIS 15914-2:2024(en) © ISO 2024

© ISO 2024 – All rights reserved
ii

---------------------- Page: 2 ----------------------
ISO/FDIS 15914-2:2024(en)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 1
6 Equipment . 2
7 Sampling . 3
8 Preparation of the sample for testing .
...

ISO/FDIS 15914-2:2023(E)
Date: 2023-10-13
ISO/TC 34/SC 10
Formatted: English (United States)
Secretariat: INSO
Date: 2024-02-19
Animal feeding stuffs — Enzymatic determination of total starch
content —
Part 2:
Method by enzymatic determination with a hexokinase system and
potassium hydroxide dispersion
Alimentation animale — Détermination enzymatique de la teneur totale en amidon —
Partie 2: Méthode par dosage enzymatique avec un système hexokinase et dispersion à l’hydroxyde de
potassium
FDIS stage

---------------------- Page: 1 ----------------------
ISO/FDIS 15914-2:2024(en)
© ISO 20232024
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication
may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying,
or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO
at the address below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: + 41 22 749 01 11
E-mail: [e-mail]
Website: www.iso.org
Field Code Changed
Published in Switzerland
© ISO 2024 – All rights reserved

ii

---------------------- Page: 2 ----------------------
ISO/FDIS 15914-2:2024(en)
Contents
Foreword . iv
1 Scope .1
2 Normative references .1
3 Terms and definitions .1
4 Principle .1
5 Reagents .1
6 Equipment . Error! Bookmark not defined.
7 Sampling .3
8 Preparation of the sample for testing .3
9 Operating procedure .3
9.1 Test sample .3
9.2 Washing, dispersion and hydrolysis .3
9.3 Determination of glucose .3
9.4 Control of the efficiency of the enzyme .4
10 Expression of the results .4
11 Precision .5
11.1 Interlaboratory test .5
11.2 Repeatability .5
11.3 Reproducibility .
...