ISO 30024:2024
(Main)Animal feeding stuffs — Determination of phytase activity
Animal feeding stuffs — Determination of phytase activity
This document specifies the determination of phytase activity in feeding stuff samples, including feed raw materials from plant origin, compound feeds (complete, complementary, mineral feeds), premixtures and feed additives. The method is applicable to, and is collaboratively validated for, the determination of phytase activity in complete feed, complementary feed including mineral feed, premixtures and feed additives. The method does not distinguish between phytase added as a feed additive and endogenous phytase already present in the feed materials. Therefore, the method is also applicable for feed materials from plant origin. The method does not apply to evaluating or comparing the in vivo efficacy of the phytase product. It is not a predictive method of the in vivo efficacy of phytases present on the market as they can develop different in vivo efficacy per unit of activity.
Alimentation animale — Détermination de l'activité phytasique
Le présent document spécifie la détermination de l’activité phytasique dans les échantillons d’aliments pour animaux, y compris dans les matières premières d’origine végétale, aliments composés (complets, complémentaires et minéraux), prémélanges et additifs destinés à l’alimentation animale. La méthode est applicable et validée de manière collaborative pour la détermination de l’activité phytasique dans les aliments complets, les aliments complémentaires, y compris les aliments minéraux, les prémélanges et les additifs, destinés à l’alimentation animale. La méthode ne fait pas la distinction entre la phytase ajoutée en tant qu’additif destiné à l’alimentation animale et la phytase endogène déjà présente dans les matières premières pour l’alimentation animale. Par conséquent, la méthode est également applicable aux matières premières d’origine végétale pour l’alimentation animale. La méthode ne s’applique pas pour évaluer ou comparer l’efficacité in vivo des phytases. Il ne s’agit pas d’une méthode prédictive de l’efficacité in vivo des phytases disponibles sur le marché, car celles-ci peuvent développer une efficacité in vivo différente par unité d’activité.
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International
Standard
ISO 30024
Second edition
Animal feeding stuffs —
2024-04
Determination of phytase activity
Alimentation animale — Détermination de l'activité phytasique
Reference number
ISO 30024:2024(en) © ISO 2024
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ISO 30024:2024(en)
COPYRIGHT PROTECTED DOCUMENT
© ISO 2024
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
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Published in Switzerland
© ISO 2024 – All rights reserved
ii
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ISO 30024:2024(en)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Reagents . 3
6 Apparatus . 5
7 Sampling and sample preparation . 5
8 Sample extractions . 6
8.1 For compound feeds (excluding mineral feeds) .6
8.2 For mineral feeds and premixtures .6
8.3 For feed additives .6
9 Procedure . 7
9.1 General .7
9.2 Blank solution .7
9.3 Standards .7
9.3.1 Phosphate standard solution.
...
Norme
internationale
ISO 30024
Deuxième édition
Alimentation animale —
2024-04
Détermination de l'activité
phytasique
Animal feeding stuffs — Determination of phytase activity
Numéro de référence
ISO 30024:2024(fr) © ISO 2024
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ISO 30024:2024(fr)
DOCUMENT PROTÉGÉ PAR COPYRIGHT
© ISO 2024
Tous droits réservés. Sauf prescription différente ou nécessité dans le contexte de sa mise en œuvre, aucune partie de cette
publication ne peut être reproduite ni utilisée sous quelque forme que ce soit et par aucun procédé, électronique ou mécanique,
y compris la photocopie, ou la diffusion sur l’internet ou sur un intranet, sans autorisation écrite préalable. Une autorisation peut
être demandée à l’ISO à l’adresse ci-après ou au comité membre de l’ISO dans le pays du demandeur.
ISO copyright office
Case postale 401 • Ch. de Blandonnet 8
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Publié en Suisse
© ISO 2024 – Tous droits réservés
ii
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ISO 30024:2024(fr)
Sommaire Page
Avant-propos .iv
Introduction .v
1 Domaine d’application . 1
2 Références normatives . 1
3 Termes et définitions . 1
4 Principe. 3
5 Réactifs . 3
6 Appareillage . 5
7 Échantillonnage et préparation des échantillons . 6
8 Extraction des échantillons . 6
8.1 Pour les aliments composés (hors aliments minéraux) .6
8.2 Pour les aliments minéraux et les prémélanges .7
8.3 Pour les additifs .7
9 Mode opératoire . 7
9.1 Généralités .7
9.2 Solution à blanc .8
9.3 Étalons .8
9.3.1 Solution étalon de phosphate .
...
FINAL DRAFT
International
Standard
ISO/FDIS 30024
ISO/TC 34/SC 10
Animal feeding stuffs —
Secretariat: INSO
Determination of phytase activity
Voting begins on:
Alimentation animale — Détermination de l'activité phytasique 2024-01-24
Voting terminates on:
2024-03-20
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WITH THEIR COMMENTS, NOTIFICATION OF ANY
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ISO/CEN PARALLEL PROCESSING LOGICAL, COMMERCIAL AND USER PURPOSES, DRAFT
INTERNATIONAL STANDARDS MAY ON OCCASION HAVE
TO BE CONSIDERED IN THE LIGHT OF THEIR POTENTIAL
TO BECOME STAN DARDS TO WHICH REFERENCE MAY BE
MADE IN NATIONAL REGULATIONS.
Reference number
ISO/FDIS 30024:2024(en) © ISO 2024
---------------------- Page: 1 ----------------------
FINAL DRAFT
ISO/FDIS 30024:2024(en)
International
Standard
ISO/FDIS 30024
ISO/TC 34/SC 10
Animal feeding stuffs —
Secretariat: INSO
Determination of phytase activity
Voting begins on:
Alimentation animale — Détermination de l'activité phytasique
Voting terminates on:
RECIPIENTS OF THIS DRAFT ARE INVITED TO SUBMIT,
COPYRIGHT PROTECTED DOCUMENT
WITH THEIR COMMENTS, NOTIFICATION OF ANY
RELEVANT PATENT RIGHTS OF WHICH THEY ARE AWARE
AND TO PROVIDE SUPPOR TING DOCUMENTATION.
© ISO 2024
IN ADDITION TO THEIR EVALUATION AS
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO
ISO/CEN PARALLEL PROCESSING
LOGICAL, COMMERCIAL AND USER PURPOSES, DRAFT
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
INTERNATIONAL STANDARDS MAY ON OCCASION HAVE
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
TO BE CONSIDERED IN THE LIGHT OF THEIR POTENTIAL
or ISO’s member body in the country of the requester.
TO BECOME STAN DARDS TO WHICH REFERENCE MAY BE
MADE IN NATIONAL REGULATIONS.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland Reference number
ISO/FDIS 30024:2024(en) © ISO 2024
© ISO 2024 – All rights reserved
ii
---------------------- Page: 2 ----------------------
ISO/FDIS 30024:2024(en)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Reagents . 3
6 Apparatus . 5
7 Sampling and sample preparation . 5
8 Sample extractions . 6
8.1 For compound feeds (excluding mineral feeds) .
...
ISO/FDIS 30024:2023(E)
Date: 2023-09-25
ISO/TC 34/SC 10/WG 12
Secretariat: INSO
Date: 2024-01-10
Animal feeding stuffs — Determination of phytase activity
Alimentation animale — Détermination de l'activité phytasique
For FDIS stage
---------------------- Page: 1 ----------------------
ISO/FDIS 30024:2024(E)
© ISO 2024
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this
publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical,
including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can
be requested from either ISO at the address below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: + 41 22 749 01 11
E-mail: copyright@iso.org
Website: www.iso.org
Published in Switzerland
© ISO 2024 – All rights reserved
ii
---------------------- Page: 2 ----------------------
ISO/FDIS 30024:2024(E)
Contents
Foreword . v
Introduction . vi
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 3
5 Reagents . 3
6 Apparatus . 5
7 Sampling and sample preparation . 6
8 Sample extractions . 6
8.1 For compound feeds (excluding mineral feeds). 6
8.2 For mineral feeds and premixtures . 6
8.3 For feed additives . 6
9 Procedure . 7
9.1 General . 7
9.2 Blank solution . 7
9.3 Standards . 7
9.3.1 Phosphate standard solution . 7
9.3.2 Phytase level control . 8
9.4 Standard curve . 8
9.5 Phytase level control . 9
9.6 For compound feed (excluding mineral feeds) .
...
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