ISO/DTS 15213-3
(Main)Microbiology of the food chain — Horizontal method for the detection and enumeration of Clostridium spp. — Part 3: Detection of Clostridium perfringens
Microbiology of the food chain — Horizontal method for the detection and enumeration of Clostridium spp. — Part 3: Detection of Clostridium perfringens
Microbiologie de la chaîne alimentaire — Méthode horizontale pour la recherche et le dénombrement de Clostridium spp. — Partie 3: Recherche de Clostridium perfringens
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FINAL
TECHNICAL ISO/DTS
DRAFT
SPECIFICATION 15213-3
ISO/TC 34/SC 9
Microbiology of the food chain —
Secretariat: AFNOR
Horizontal method for the detection
Voting begins on:
2023-10-13 and enumeration of Clostridium
spp. —
Voting terminates on:
2024-01-05
Part 3:
Detection of Clostridium perfringens
Microbiologie de la chaîne alimentaire — Méthode horizontale pour
la recherche et le dénombrement de Clostridium spp. —
Partie 3: Recherche de Clostridium perfringens
ISO/CEN PARALLEL PROCESSING
RECIPIENTS OF THIS DRAFT ARE INVITED TO
SUBMIT, WITH THEIR COMMENTS, NOTIFICATION
OF ANY RELEVANT PATENT RIGHTS OF WHICH
THEY ARE AWARE AND TO PROVIDE SUPPOR TING
DOCUMENTATION.
IN ADDITION TO THEIR EVALUATION AS
Reference number
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO-
ISO/DTS 15213-3:2023(E)
LOGICAL, COMMERCIAL AND USER PURPOSES,
DRAFT INTERNATIONAL STANDARDS MAY ON
OCCASION HAVE TO BE CONSIDERED IN THE
LIGHT OF THEIR POTENTIAL TO BECOME STAN-
DARDS TO WHICH REFERENCE MAY BE MADE IN
NATIONAL REGULATIONS. © ISO 2023
---------------------- Page: 1 ----------------------
ISO/DTS 15213-3:2023(E)
FINAL
TECHNICAL ISO/DTS
DRAFT
SPECIFICATION 15213-3
ISO/TC 34/SC 9
Microbiology of the food chain —
Secretariat: AFNOR
Horizontal method for the detection
Voting begins on:
and enumeration of Clostridium
spp. —
Voting terminates on:
Part 3:
Detection of Clostridium perfringens
Microbiologie de la chaîne alimentaire — Méthode horizontale pour
la recherche et le dénombrement de Clostridium spp. —
Partie 3: Recherche de Clostridium perfringens
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© ISO 2023
ISO/CEN PARALLEL PROCESSING
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
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ISO copyright office
SUBMIT, WITH THEIR COMMENTS, NOTIFICATION
OF ANY RELEVANT PATENT RIGHTS OF WHICH
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THEY ARE AWARE AND TO PROVIDE SUPPOR TING
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DOCUMENTATION.
Phone: +41 22 749 01 11
IN ADDITION TO THEIR EVALUATION AS
Reference number
Email: copyright@iso.org
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO-
ISO/DTS 15213-3:2023(E)
Website: www.iso.org
LOGICAL, COMMERCIAL AND USER PURPOSES,
DRAFT INTERNATIONAL STANDARDS MAY ON
Published in Switzerland
OCCASION HAVE TO BE CONSIDERED IN THE
LIGHT OF THEIR POTENTIAL TO BECOME STAN-
DARDS TO WHICH REFERENCE MAY BE MADE IN
ii
© ISO 2023 – All rights reserved
NATIONAL REGULATIONS. © ISO 2023
---------------------- Page: 2 ----------------------
ISO/DTS 15213-3:2023(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 2
3 Terms and definitions . 2
4 Principle . 3
4.1 General . 3
4.2 Enrichment in selective liquid medium . . 3
4.3 Isolation on selective solid medium . 3
4.4 Confirmation .
...
nd
2 draft after pre-DTS ballot for ISO/DTS 15213-3:2023(E)
ISO/TC 34/SC 9/WG 23/N 245
Secretariat: NENAFNOR
Date: 2023-07-0509-28
Microbiology of the food chain — Horizontal method for the detection and enumeration of
Clostridium spp. — Part 3: Detection of Clostridium perfringens
DRAFT DTS stage
Warning for NPs, WDs and CDs
This document is not an ISO International Standard. It is distributed for review and comment. It is subject to
change without notice and may not be referred to as an International Standard.
Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of
which they are aware and to provide supporting documentation.
---------------------- Page: 1 ----------------------
© ISO 2023
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or
utilized otherwise in any form or by any means, electronic or mechanical, including photocopying,
or posting on the internet or an intranet, without prior written permission. Permission can be
requested from either ISO at the address below or ISO's member body in the country of the
requester.
ISO copyright office
Case postale 56 •• CH-1211 Geneva 20
Tel. + 41 22 749 01 11
Fax + 41 22 749 09 47
E-mail copyright@iso.org
Web www.iso.org
Web www.iso.org
Published in Switzerland.
---------------------- Page: 2 ----------------------
nd
2 draft after pre-DTS ballot for ISO/DTS 15213-3
Contents
Foreword . 6
Introduction. 8
1 Scope . 1
2 Normative references . 2
3 Terms and definitions . 2
4 Principle . 3
4.1 General . 3
4.2 Enrichment in selective liquid medium . 3
4.3 Isolation on selective solid medium . 3
4.4 Confirmation . 3
5 Culture media and reagents . 3
6 Equipment and consumables . 3
7 Sampling . 4
8 Preparation of test sample . 4
9 Procedure. 5
9.1 General . 5
9.2 Test portion and initial suspension . 5
9.3 Selective enrichment . 5
9.4 Isolation . 5
9.5 Confirmation of C. perfringens .
...
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