ISO/TS 15213-3

Microbiology of the food chain — Horizontal method for the detection and enumeration of Clostridium spp. — Part 3: Detection of Clostridium perfringens

This document specifies the detection of Clostridium (C.) perfringens. This document is applicable to: — products intended for human consumption; — products intended for animal feeding; — environmental samples in the area of food and feed production and handling; — samples from the primary production stage. This horizontal method was originally developed for the examination of all samples belonging to the food chain. Based on the information available at the time of publication of this document, this method is considered to be fully suited to the examination of all samples belonging to the food chain. However, because of the large variety of products in the food chain, it is possible that this horizontal method is not appropriate in every detail for all products. Nevertheless, it is expected that the required modifications are minimized so that they do not result in a significant deviation from this horizontal method. NOTE Interlaboratory studies with a small number of participating laboratories ( — ready-to-eat, ready-to-reheat meat products; — eggs and egg products (derivates); — ready-to-eat, ready-to-reheat fishery products; — processed fruits and vegetables; — infant formula and infant cereals (with probiotics); — multi-component foods or meal components. It has also been validated with a small number of participating laboratories for the following other category: — environmental samples (food or feed production). Since the method is not commonly used for samples in the primary production stage, this category was not included in the interlaboratory study. Therefore, no performance characteristics were obtained for this category. The method has not been validated for the category ‘pet food and animal feed’, as the test samples used for the interlaboratory study were already naturally contaminated with C. perfringens. Given the limited number of participating laboratories in the interlaboratory studies, the calculated performance characteristics can be used as indicative values of the method performance. For detailed information on the validation, see Clause 11 and Annexes C to F.

Microbiologie de la chaîne alimentaire — Méthode horizontale pour la recherche et le dénombrement de Clostridium spp. — Partie 3: Recherche de Clostridium perfringens

Le présent document définit la recherche de Clostridium (C.) perfringens. Le présent document s’applique: — aux produits destinés à la consommation humaine; — aux produits destinés à l’alimentation animale; — aux échantillons environnementaux prélevés dans les secteurs de la production et de la distribution des aliments; — aux échantillons issus du stade de la production primaire. Cette méthode horizontale a été initialement développée pour l’examen de tous les échantillons provenant de la chaîne alimentaire. Sur la base des informations disponibles au moment de la publication du présent document, cette méthode est considérée comme parfaitement adaptée à l’examen de tous les échantillons provenant de la chaîne alimentaire. Cependant, en raison de la grande diversité des produits de la chaîne alimentaire, il est possible que cette méthode horizontale ne soit pas appropriée dans ses moindres détails à tous les produits. Néanmoins, il est attendu que les modifications requises soient réduites au minimum afin qu’elles n’entraînent pas de déviation significative de cette méthode horizontale. NOTE Des études interlaboratoires avec un petit nombre de laboratoires participants ( — les produits à base de viande prêts à consommer et prêts à réchauffer; — les œufs et ovoproduits (dérivés); — les produits à base de poisson prêts à consommer et prêts à réchauffer; — les fruits et légumes transformés; — les préparations et céréales pour nourrissons (avec probiotiques); — les aliments composés et les composants de repas. La méthode a également été validée avec un petit nombre de laboratoires participants pour la catégorie suivante: — les échantillons environnementaux (production d’aliments ou d’aliments pour animaux). Étant donné que cette méthode n’est pas couramment utilisée pour les échantillons au stade de la production primaire, cette catégorie n’a pas été incluse dans l’étude interlaboratoires. Par conséquent, aucune caractéristique de performance n’a été déterminée pour cette catégorie. La méthode n’a pas été validée pour la catégorie «aliments pour animaux», car les échantillons pour essai utilisés dans l’étude interlaboratoires étaient déjà naturellement contaminés par C. perfringens. Étant donné le nombre limité de laboratoires participants dans les études interlaboratoires, les caractéristiques de performance calculées peuvent être utilisées comme valeurs indicatives pour la performance de la méthode. Pour de plus amples informations sur la validation, voir l’Article 11 et les Annexes C à F.

General Information

• Status: Not Published
• ICS: 07.100.30 - Food microbiology
• Technical Committee: ISO/TC 34/SC 9 - Microbiology
• Drafting Committee: ISO/TC 34/SC 9/WG 23 - Sulfite reducing clostridia and C. perfringens
• Current Stage: 6000 - International Standard under publication
• Completion Date: 24-Jan-2024

Relations

• Consolidates: ISO 18541-5:2018 - Road vehicles — Standardized access to automotive repair and maintenance information (RMI) — Part 5: Heavy duty specific provision
• Effective Date: 06-Jun-2022
• Revised: ISO 15213:2003 - Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of sulfite-reducing bacteria growing under anaerobic conditions
• Effective Date: 04-Jun-2016

Standards Content (Sample)

FINAL
TECHNICAL ISO/DTS
DRAFT
SPECIFICATION 15213-3
ISO/TC 34/SC 9
Microbiology of the food chain —
Secretariat: AFNOR
Horizontal method for the detection
Voting begins on:
2023-10-13 and enumeration of Clostridium
spp. —
Voting terminates on:
2024-01-05
Part 3:
Detection of Clostridium perfringens
Microbiologie de la chaîne alimentaire — Méthode horizontale pour
la recherche et le dénombrement de Clostridium spp. —
Partie 3: Recherche de Clostridium perfringens
ISO/CEN PARALLEL PROCESSING
RECIPIENTS OF THIS DRAFT ARE INVITED TO
SUBMIT, WITH THEIR COMMENTS, NOTIFICATION
OF ANY RELEVANT PATENT RIGHTS OF WHICH
THEY ARE AWARE AND TO PROVIDE SUPPOR TING
DOCUMENTATION.
IN ADDITION TO THEIR EVALUATION AS
Reference number
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO-
ISO/DTS 15213-3:2023(E)
LOGICAL, COMMERCIAL AND USER PURPOSES,
DRAFT INTERNATIONAL STANDARDS MAY ON
OCCASION HAVE TO BE CONSIDERED IN THE
LIGHT OF THEIR POTENTIAL TO BECOME STAN-
DARDS TO WHICH REFERENCE MAY BE MADE IN
NATIONAL REGULATIONS. © ISO 2023

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ISO/DTS 15213-3:2023(E)
FINAL
TECHNICAL ISO/DTS
DRAFT
SPECIFICATION 15213-3
ISO/TC 34/SC 9
Microbiology of the food chain —
Secretariat: AFNOR
Horizontal method for the detection
Voting begins on:
and enumeration of Clostridium
spp. —
Voting terminates on:
Part 3:
Detection of Clostridium perfringens
Microbiologie de la chaîne alimentaire — Méthode horizontale pour
la recherche et le dénombrement de Clostridium spp. —
Partie 3: Recherche de Clostridium perfringens
COPYRIGHT PROTECTED DOCUMENT
© ISO 2023
ISO/CEN PARALLEL PROCESSING
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
RECIPIENTS OF THIS DRAFT ARE INVITED TO
ISO copyright office
SUBMIT, WITH THEIR COMMENTS, NOTIFICATION
OF ANY RELEVANT PATENT RIGHTS OF WHICH
CP 401 • Ch. de Blandonnet 8
THEY ARE AWARE AND TO PROVIDE SUPPOR TING
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DOCUMENTATION.
Phone: [phone]
IN ADDITION TO THEIR EVALUATION AS
Reference number
Email: [e-mail]
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO-
ISO/DTS 15213-3:2023(E)
Website: www.iso.org
LOGICAL, COMMERCIAL AND USER PURPOSES,
DRAFT INTERNATIONAL STANDARDS MAY ON
Published in Switzerland
OCCASION HAVE TO BE CONSIDERED IN THE
LIGHT OF THEIR POTENTIAL TO BECOME STAN-
DARDS TO WHICH REFERENCE MAY BE MADE IN
ii
  © ISO 2023 – All rights reserved
NATIONAL REGULATIONS. © ISO 2023

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ISO/DTS 15213-3:2023(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 2
3 Terms and definitions . 2
4 Principle . 3
4.1 General . 3
4.2 Enrichment in selective liquid medium . . 3
4.3 Isolation on selective solid medium . 3
4.4 Confirmation .
...

nd
2 draft after pre-DTS ballot for ISO/DTS 15213-3:2023(E)
ISO/TC 34/SC 9/WG 23/N 245
Secretariat: NENAFNOR
Date: 2023-07-0509-28
Microbiology of the food chain — Horizontal method for the detection and enumeration of
Clostridium spp. — Part 3: Detection of Clostridium perfringens

DRAFT DTS stage

Warning for NPs, WDs and CDs
This document is not an ISO International Standard. It is distributed for review and comment. It is subject to
change without notice and may not be referred to as an International Standard.
Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of
which they are aware and to provide supporting documentation.

---------------------- Page: 1 ----------------------
© ISO 2023
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or
utilized otherwise in any form or by any means, electronic or mechanical, including photocopying,
or posting on the internet or an intranet, without prior written permission. Permission can be
requested from either ISO at the address below or ISO's member body in the country of the
requester.
ISO copyright office
Case postale 56 •• CH-1211 Geneva 20
Tel. + 41 22 749 01 11
Fax + 41 22 749 09 47
E-mail [e-mail]
Web www.iso.org
Web www.iso.org
Published in Switzerland.

---------------------- Page: 2 ----------------------
nd
2 draft after pre-DTS ballot for ISO/DTS 15213-3

Contents
Foreword . 6
Introduction. 8
1 Scope . 1
2 Normative references . 2
3 Terms and definitions . 2
4 Principle . 3
4.1 General . 3
4.2 Enrichment in selective liquid medium . 3
4.3 Isolation on selective solid medium . 3
4.4 Confirmation . 3
5 Culture media and reagents . 3
6 Equipment and consumables . 3
7 Sampling . 4
8 Preparation of test sample . 4
9 Procedure. 5
9.1 General . 5
9.2 Test portion and initial suspension . 5
9.3 Selective enrichment . 5
9.4 Isolation . 5
9.5 Confirmation of C. perfringens .
...