IWA 15:2015
(Main)Specification and method for the determination of performance of automated liquid handling systems
Specification and method for the determination of performance of automated liquid handling systems
IWA 15:2015 specifies methods for testing the volumetric performance of air-displacement, system-liquid filled and positive displacement automated liquid handling systems (ALHS), including an estimation of measurement uncertainties and established traceability to reference standards (preferably, traceability to SI Units). The testing methods specified in this document may also be used to measure the volumetric performance of automated liquid delivery systems which do not aspirate the test liquid. IWA 15:2015 also specifies statistical methods for the determination of random and systematic errors (including intra-plate and inter-plate comparisons), analysis of measured results when using multichannel dispensing heads, and analysis depending on dispense patterns. It further defines terms and formulae to be used for summarizing test results. IWA 15:2015 also specifies the information to be provided to users of ALHS, including the display of summary results and performance claims. IWA 15:2015 is applicable to all ALHS with complete, installed liquid handling devices, including tips and other essential parts needed for delivering a specified volume, which perform liquid handling tasks without human intervention into microplates. Manipulation of the microplates on the deck of the automated liquid handling system may be achieved automatically, semi-automatically, or manually. IWA 15:2015 addresses the needs of: - suppliers of ALHS, as a basis for quality control including, where appropriate, the issuance of supplier's declarations; - test houses and other bodies, as a basis for independent certification and calibration; - users of the equipment, to enable calibration, verification, validation, and routine testing of trueness and precision. The tests established in this IWA should be carried out by trained personnel.
Spécification et méthode pour la détermination de performance des systèmes automatisés de manipulation de liquides
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Standards Content (Sample)
INTERNATIONAL IWA
WORKSHOP 15
AGREEMENT
First edition
2015-10-15
Specification and method for the
determination of performance of
automated liquid handling systems
Spécification et méthode pour la détermination de performance des
systèmes automatisés de manipulation de liquides
Reference number
©
ISO 2015
© ISO 2015, Published in Switzerland
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ii © ISO 2015 – All rights reserved
Contents Page
Foreword .v
1 Scope .1
2 Normative references .1
3 Terms and definitions .1
3.1 Definitions . 2
3.2 Abbreviated terms . 8
4 Operation of automated liquid handling systems .9
4.1 Types of automated liquid handling systems . 9
4.1.1 Types of piston operated automated liquid handling systems . 9
4.1.2 Types of other (pump operated) automated liquid handling systems. 9
4.2 Adjustment .10
4.2.1 Need for adjustment .10
4.2.2 Liquid classes .10
4.2.3 Adjustment of ALHS settings .10
4.3 Tips .10
4.3.1 General.10
4.3.2 Air-displacement tips .10
4.3.3 Positive displacement tips .11
4.3.4 Fixed tips.11
4.4 Environmental conditions .11
4.4.1 Discussion and recommendations.11
4.4.2 Factory acceptance testing .11
4.4.3 Site acceptance and user testing .12
5 Volumetric performance .12
5.1 Introductory discussion .12
5.2 Data collection and examination .14
5.3 Indexing to track data .15
5.3.1 Indexing from the channel perspective .15
5.3.2 Indexing from the microplate perspective .15
5.4 Descriptive statistics on an individual channel basis .16
5.5 Descriptive statistics on a run order basis .18
5.6 Descriptive statistics for entire data sets .18
5.7 Differences between channels .19
5.8 Handling of sub-deliveries .19
6 Measurement methods .20
6.1 Overview of methods suitable for measuring ALHS performance .20
6.2 Methods for use with any ALHS platform (open methods) .29
6.2.1 Ratiometric photometry .29
6.2.2 Gravimetry, single channel measurement .29
6.2.3 Gravimetry, full-plate measurement for correlation with photometry .29
6.2.4 Gravimetric regression method for low volumes .30
6.2.5 Photometry using Orange G .30
6.2.6 Hybrid method: gravimetry and photometry with Tartrazine . .30
6.2.7 Hybrid method: photometry and gravimetry with p-nitrophenol .31
6.2.8 Hybrid method: gravimetry and photometry with Ponceau S .31
6.2.9 Pressure sensing .31
6.2.10 Calorimetric measurement .31
6.2.11 Optical image analysis .31
6.3 Methods specific to an ALHS model or accessory (closed methods) .32
6.3.1 Gravimetry and hybrid method: gravimetry and photometry .32
6.3.2 Photometry with Tartrazine .32
7 Specification of ALHS volumetric performance .33
7.1 Mandatory information to be supplied by manufacturer .33
7.2 Optional information that can be supplied by manufacturer .33
8 Reporting .34
8.1 Reporting the results .34
8.1.1 General.34
8.1.2 Test reports and calibration certificates .34
8.1.3 Calibration certificates.35
9 Potential future work .36
Annex A (informative) Applications of descriptive statistics .38
Annex B (informative) Test methods for ALHS performance .50
Annex C (normative) Calculation of liquid volumes from balance readings .114
Annex D (informative) Workshop contributors .116
Bibliography .117
iv © ISO 2015 – All rights reserved
Foreword
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The committee responsible for this document is ISO/TMBG, Technical Management Board Groups.
International Workshop Agreement IWA 15:2015(E)
Specification and method for the determination of
performance of automated liquid handling systems
1 Scope
This International Workshop Agreement (IWA) specifies methods for testing the volumetric
performance of air-displacement, system-liquid filled and positive displacement automated liquid
handling systems (ALHS), including an estimation of measurement uncertainties and established
traceability to reference standards (preferably, traceability to SI Units). The testing methods specified
in this document may also be used to measure the volumetric performance of automated liquid delivery
systems
...
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