ISO 14939:2001

Animal feeding stuffs — Determination of carbadox content — Method using high-performance liquid chromatography

This International Standard specifies a high-performance liquid chromatographic (HPLC) method for the determination of the carbadox content in premixtures and animal feeding stuffs. The method is applicable to animal feeding stuffs with a mass fraction of carbadox of 0,5 mg/kg (limit of quantification) to 100 mg/kg, and to premixtures with a mass fraction of carbadox up to 10 %. The lower limit of detection is 0,1 mg/kg. NOTE 1 For animal feeding stuffs the mass fraction of carbadox is expressed in milligrams per kilogram, and for premixtures as a percentage by mass. NOTE 2 Carbadox is a chemotherapeuticum belonging to the quinoxaline group. Carbadox is used as a growth-promoting feed additive for piglets.

Aliments des animaux — Détermination de la teneur en carbadox — Méthode par chromatographie liquide à haute performance

General Information

• Status: Published
• Publication Date: 29-Aug-2001
• ICS: 65.120 - Animal feeding stuffs
• Technical Committee: ISO/TC 34/SC 10 - Animal feeding stuffs
• Drafting Committee: ISO/TC 34/SC 10 - Animal feeding stuffs
• Current Stage: 9060 - Close of review
• Start Date: 02-Sep-2029

Standards Content (Sample)

INTERNATIONAL ISO
STANDARD 14939
First edition
2001-08-15
Animal feeding stuffs — Determination of
carbadox content — Method using high-
performance liquid chromatography
Aliments des animaux — Détermination de la teneur en carbadox —
Méthode par chromatographie liquide à haute performance
Reference number
ISO 14939:2001(E)
©
ISO 2001

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ISO 14939:2001(E)
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ISO 14939:2001(E)
Contents Page
Foreword.iv
1 Scope .1
2 Normative reference .1
3 Principle.1
4 Reagents.2
5 Apparatus .4
6 Sampling.5
7 Preparation of test sample.5
8 Procedure .5
8.1 General.5
8.2 Preparation of spiked sample.5
8.3 Extraction .6
8.4 Column chromatography.7
8.5 HPLC analysis.7
9 Confirmation.8
9.1 General.8
9.2 Co-chromatography.8
9.3 Diode array detector.9
9.4 Post-column derivatization.
...