ISO 19056-1:2015
(Main)Microscopes — Definition and measurement of illumination properties — Part 1: Image brightness and uniformity in bright field microscopy
Microscopes — Definition and measurement of illumination properties — Part 1: Image brightness and uniformity in bright field microscopy
ISO 19056-1:2015 specifies procedures for the measurement of image brightness and uniformity for bright field light microscopy. These measurements for image brightness and uniformity are defined in image planes or intermediate image planes only, when these planes are suitable for detection by electronic imaging devices. ISO 19056-1:2015 defines how image brightness and uniformity are measured and how this information is provided to the user. NOTE The scope is intentionally limited to electronic imaging devices and (intermediate) image planes. The visual observation by means of eyepieces would require a different measurement procedure and hence result in unambiguities in the description of measurement procedures. Nevertheless, this part of ISO 19056 will give useful estimates for the image brightness with visual observation as in this case, an eyepiece is used to observe an intermediate image plane (which is under the scope of this part of ISO 19056).
Microscopes — Définition et mesurage des propriétés d'éclairage — Partie 1: Luminosité et uniformité de l'image en microscopie à champ clair
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Standards Content (Sample)
INTERNATIONAL ISO
STANDARD 19056-1
First edition
2015-09-15
Microscopes — Definition and
measurement of illumination
properties —
Part 1:
Image brightness and uniformity in
bright field microscopy
Microscopes — Définition et mesurage des propriétés d’éclairage —
Partie 1: Luminosité et uniformité de l’image en microscopie à champ
clair
Reference number
ISO 19056-1:2015(E)
©
ISO 2015
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ISO 19056-1:2015(E)
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ii © ISO 2015 – All rights reserved
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ISO 19056-1:2015(E)
Contents Page
Foreword .iv
1 Scope . 1
2 Measurands . 1
2.1 General . 1
2.2 Brightness . 1
2.3 Uniformity . 2
3 Measurement procedure . 2
3.1 General . 2
3.2 Diaphragm settings . 2
3.3 Brightness (illuminance and irradiance) . 2
3.4 Uniformity . 3
3.5 Spectral information . 3
4 Information provided to the user . 3
Bibliography . 5
© ISO 2015 – All rights reserved iii
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ISO 19056-1:2015(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
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described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
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Barriers to Trade (TBT) see the following URL: Foreword - Supplementary information
The committee responsible for this document is ISO/TC 172, Optics and photonics, Subcommittee SC 5,
Microscopes and endoscopes.
ISO 19056 consists of the following parts, under the general title Microscopes — Definition and
measurement of illumination properties:
— Part 1: Image brightness and uniformity in bright field microscopy
iv © ISO 2015 – All rights reserved
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INTERNATIONAL STANDARD ISO 19056-1:2015(E)
Microscopes — Definition and measurement of
illumination properties —
Part 1:
Image brightness and uniformity in bright field microscopy
1 Scope
This part of ISO 19056 specifies procedures for the measurement of image brightness and uniformity
for bright field light microscopy. These measurements for image brightness and uniformity are defined
in image planes or intermediate image planes only, when these planes are suitable for detection by
electronic imaging devices.
This part of ISO 19056 defines how image brightness and uniformity are measured and how this
information is provided to the user.
NOTE The scope is intentionally limited to electronic imaging devices and (intermediate) image planes. The
visual observation by means of eyepieces would require a different measurement procedure and hence result
in unambiguities in the description of measurement procedures. Nevertheless, this part of ISO 19056 will give
useful estimates for the image brightness with visual observation as in this case, an eyepiece is used to observe
an intermediate image plane (which is under the scope of this part of ISO 19056).
2 Measurands
2.1 General
For obtaining the desired image quality when using a light microscope, the brightness and
uniformity of the image play an important role. This holds true for different applications and
various types of instruments.
Intentionally, this part of ISO 19056 is limited to bright field microscopy and detection by means of
electronic imaging devices but within this limitation, this part of ISO 19056 does apply to tra
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