SIST EN ISO 15302:2007
(Main)Animal and vegetable fats and oils - Determination of benzo[a]pyrene content - Reverse-phase high-performance liquid chromatography method (ISO 15302:1998)
Animal and vegetable fats and oils - Determination of benzo[a]pyrene content - Reverse-phase high-performance liquid chromatography method (ISO 15302:1998)
Specifies a method for the determination of benzo[a]pyrene in crude or refined edible oils and fats by reverse-phase high-performance liquid chromatography (HPLC) using fluorimetric detection in the range from 0,1 µg/kg to 10 µg/kg.
Tierische und pflanzliche Fette und Öle - Bestimmung des Benzo[a]pyren-Gehalts - Umkehrphasen-HPLC-Verfahren (ISO 15302:1998)
Diese Internationale Norm legt ein Verfahren für die Bestimmung von Benzo[a]pyren in rohen oder veredelten
essbaren Ölen und Fetten durch Hochleistungsflüssigchromatographie mit umgekehrten Phasen (HPLC)
unter Anwendung eines fluorometrischen Nachweises in dem Bereich von 0,1 µg/kg bis 10 µg/kg fest.
Corps gras d'origines animale et végétale - Détermination de la teneur en benzo[a]pyrene - Méthode par chromatographie liquide a haute performance a polarité de phase inversée (ISO 15302:1998)
L'IEC 60704-2-16:2019 s'applique aux lavantes-séchantes électriques en un seul équipement pour une utilisation domestique ou similaire prévue avec un placement au sol contre un mur, une intégration ou un placement sous un comptoir, un plan de travail de cuisine ou sous un évier, pour une fixation murale ou sur un comptoir.
La présente norme concerne des méthodes objectives de degré de précision «expertise» destinées à déterminer les niveaux de puissance acoustique d'un bruit aérien situé à l'intérieur du domaine de fréquences utile.
Les exigences pour la déclaration des valeurs d'émission sonore ne relèvent pas du domaine d'application du présent document.
La présente partie est destinée à être utilisée conjointement avec l'IEC 60704-1:2010.
Rastlinske in živalske maščobe in olja - Določevanje benzo[a]pirena - Metoda tekočinske kromatografije visoke ločljivosti z reverzno fazo (RP HPLC) (ISO 15302:1998)
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
SIST EN ISO 15302:2007
01-julij-2007
5DVWOLQVNHLQåLYDOVNHPDãþREHLQROMD'RORþHYDQMHEHQ]R>D@SLUHQD0HWRGD
WHNRþLQVNHNURPDWRJUDILMHYLVRNHORþOMLYRVWL]UHYHU]QRID]R 53+3/& ,62
Animal and vegetable fats and oils - Determination of benzo[a]pyrene content - Reverse-
phase high-performance liquid chromatography method (ISO 15302:1998)
Tierische und pflanzliche Fette und Öle - Bestimmung des Benzo[a]pyren-Gehalts -
Umkehrphasen-HPLC-Verfahren (ISO 15302:1998)
Corps gras d'origines animale et végétale - Détermination de la teneur en benzo[a]
pyrene - Méthode par chromatographie liquide a haute performance a polarité de phase
inversée (ISO 15302:1998)
Ta slovenski standard je istoveten z: EN ISO 15302:2007
ICS:
67.200.10 5DVWOLQVNHLQåLYDOVNH Animal and vegetable fats
PDãþREHLQROMD and oils
SIST EN ISO 15302:2007 en;fr;de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
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EUROPEAN STANDARD
EN ISO 15302
NORME EUROPÉENNE
EUROPÄISCHE NORM
January 2007
ICS 67.200.10
English Version
Animal and vegetable fats and oils - Determination of
benzo[a]pyrene content - Reverse-phase high-performance
liquid chromatography method (ISO 15302:1998)
Corps gras d'origines animale et végétale - Détermination Tierische und pflanzliche Fette und Öle - Bestimmung des
de la teneur en benzo[a]pyrène - Méthode par Benzo[a]pyren-Gehalts - Umkehrphasen-HPLC-Verfahren
chromatographie liquide à haute performance à polarité de (ISO 15302:1998)
phase inversée (ISO 15302:1998)
This European Standard was approved by CEN on 23 December 2006.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the
official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36 B-1050 Brussels
© 2007 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 15302:2007: E
worldwide for CEN national Members.
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EN ISO 15302:2007 (E)
Foreword
The text of ISO 15302:1998 has been prepared by Technical Committee ISO/TC 34 "Agricultural
food products” of the International Organization for Standardization (ISO) and has been taken
over as EN ISO 15302:2007 by Technical Committee CEN/TC 307 "Oilseeds, vegetable and
animal fats and oils and their by-products - Methods of sampling and analysis", the secretariat of
which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of
an identical text or by endorsement, at the latest by July 2007, and conflicting national standards
shall be withdrawn at the latest by July 2007.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of
the following countries are bound to implement this European Standard: Austria, Belgium,
Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United
Kingdom.
Endorsement notice
The text of ISO 15302:1998 has been approved by CEN as EN ISO 15302:2007 without any
modifications.
2
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INTERNATIONAL ISO
STANDARD 15302
First edition
1998-07-01
Animal and vegetable fats and oils —
Determination of benzo[a]pyrene content —
Reverse-phase high-performance liquid
chromatography method
Corps gras d'origines animale et végétale — Détermination de la teneur en
benzo[a]pyrène — Méthode par chromatographie liquide à haute
performance à polarité de phase inversée
Reference number
A
ISO 15302:1998(E)
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ISO 15302:1998(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide
federation of national standards bodies (ISO member bodies). The work of
preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which
a technical committee has been established has the right to be represented
on that committee. International organizations, governmental and non-
governmental, in liaison with ISO, also take part in the work. ISO
collaborates closely with the International Electrotechnical Commission
(IEC) on all matters of electrotechnical standardization.
Draft International Standards adopted by the technical committees are
circulated to the member bodies for voting. Publication as an International
Standard requires approval by at least 75 % of the member bodies casting
a vote.
International Standard ISO 15302 was prepared by Technical Committee
ISO/TC 34, Agricultural food products, Subcommittee SC 11, Animal and
vegetable fats and oils.
Annexes A and B of this International Standard are for information only.
© ISO 1998
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced
or utilized in any form or by any means, electronic or mechanical, including photocopying and
microfilm, without permission in writing from the publisher.
International Organization for Standardization
Case postale 56 • CH-1211 Genève 20 • Switzerland
Internet central@iso.ch
X.400 c=ch; a=400net; p=iso; o=isocs; s=central
Printed in Switzerland
ii
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©
INTERNATIONAL STANDARD ISO ISO 15302:1998(E)
Animal and vegetable fats and oils — Determination of
benzo[a]pyrene content — Reverse-phase high-performance liquid
chromatography method
1 Scope
This International Standard specifies a method for the determination of benzo[a]pyrene in crude or refined edible
oils and fats by reverse-phase high-performance liquid chromatography (HPLC) using fluorimetric detection in the
range from 0,1 μg/kg to 10 μg/kg.
2 Normative reference
The following standard contains provisions, which through reference in this text, constitute provisions of this
International Standard. At the time of publication the edition indicated was valid. All standards are subject to revision
and parties to agreements based on this International Standard are encouraged to investigate the possibility of
applying the most recent edition of the standard indicated below. Members of IEC and ISO maintain registers of
currently valid International Standards.
ISO 661:1989, Animal and vegetable fats and oils — Preparation of test sample
3 Principle
Adsorption of a suitable amount of sample on an alumina column, followed by elution with light petroleum of any
benzo[a]pyrene present, and subsequent analysis of the eluate by HPLC using a fluorimetric detector.
4 Reagents
All reagents shall be of recognized analytical grade. Where analytical grade solvents other than the recommended
ones are used, a full blank analysis shall be carried out and the results of this blank analysis reported.
4.1 Water, double distilled, filtered through a membrane filter of 0,45 μm pore size; deionized water obtained by
purifying demineralized water systems may also be used.
4.2 Light petroleum (boiling point range between 40 °C and 60 °C), or hexane, redistilled over potassium
hydroxide pellets (4 g/l).
4.3 Acetonitrile, suitable for HPLC.
4.4 Tetrahydrofuran, suitable for HPLC.
4.5 Toluene, suitable for HPLC.
4.6 Sodium sulfate, granular, anhydrous.
1
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©
ISO
ISO 15302:1998(E)
4.7 Alumina, activity grade 4, prepared from neutral aluminium oxide, activity grade super 1, deactivated by the
addition of 10 ml distilled water to 90 g of alumina.
CAUTION — THE REACTION IS EXOTHERMIC AND PRESSURE MAY BUILD UP.
Shake the container for about 15 min and allow the contents to equilibriate for 24 h. Store the alumina in a closed
vessel at ambient temperature.
4.8 Benzo[a]pyrene, 99,0 % purity.
CAUTION — BENZO[a]PYRENE IS A KNOWN CARCINOGEN. CARRY OUT ALL WORK WITH IT IN A FUME
HOOD, WEARING GLOVES TO MINIMIZE EXPOSURE.
1)
4.9 Benzo[a]pyrene solutions
4.9.1 Stock solution
Weigh, to the nearest 0,1 mg, about 12,5 mg of benzo[a]pyrene in a 25 ml graduated flask. Dissolve it in toluene
(4.5) and fill to the mark.
This solution contains about 0,5 mg/ml benzo[a]pyrene and should be stored in the dark at 4 °C when it is stable for
6 months at least.
4.9.2 Standard solutions
Prepare two standard solutions containing approximately 0,2 μg/ml and 0,01 μg/ml of benzo[a]pyrene respectively
by diluting aliquots of the stock solution (4.9.1) with acetonitrile (4.3).
5 Apparatus
Usual laboratory apparatus and, in particular, the following.
5.1 Glass column for chromatography, 300 mm long, 15 mm internal diameter, fitted with sintered glass discs,
and polytetrafluoroethylene (PTFE) tap.
5.2 Water baths (two), maintained at 35 °C – 1°C and 65 °C – 1°C.
5.3 Flash evaporator
A rotary evaporator with vacuum and a water bath set at 40 °C may be used. Care should be taken to prevent cross
contamination. Clean the system thoroughly between determinations.
5.4 High-performance liquid chromatograph, consisting of an HPLC pump, injection valve with 10 μl sample
loop, reverse-phase column, electronic integrator and chart recorder.
NOTE If an autosampler is used, the sample loop should be flushed with acetonitrile between subsequent injections.
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