Animal and vegetable fats and oils - Determination of iodine value (ISO 3961:2024)

This document specifies a reference method for the determination of the iodine value (commonly known in the industry as IV) of animal and vegetable fats and oils, hereinafter referred to as fats.
Annex B describes a method for the calculation of the IV from fatty acid compositional data. This method is not applicable to fish oils. Furthermore, cold-pressed, crude and unrefined vegetable oils as well as (partially) hydrogenated oils can give different results by the two methods. The calculated IV is affected by impurities and thermal degradation products.
NOTE The method in Annex B is based upon the AOCS Official method Cd 1c-85[10].

Tierische und pflanzliche Fette und Öle - Bestimmung der Iodzahl (ISO 3961:2024)

Dieses Dokument legt ein Bezugsverfahren zur Bestimmung der Iodzahl (im industriellen Bereich gemeinhin bekannt als IZ) in tierischen und pflanzlichen Fetten und Ölen fest, die nachfolgend als Fette bezeichnet werden.
Anhang B beschreibt ein Verfahren zur Berechnung der IZ aus Daten zur Fettsäurezusammensetzung. Dieses Verfahren ist nicht auf Fischöl anwendbar. Außerdem können kaltgepresste, unverarbeitete und nicht raffinierte pflanzliche Öle sowie (teilweise) hydrierte Öle zu unterschiedlichen Ergebnissen bei den zwei Verfahren führen. Die berechnete Iodzahl IZ wird durch Verunreinigungen und durch thermische Abbauprodukte beeinträchtigt.
ANMERKUNG   Das Verfahren in Anhang B beruht auf dem offiziellen AOCS Verfahren Cd 1c 85 [10].

Corps gras d'origines animale et végétale - Détermination de l'indice d'iode (ISO 3961:2024)

Le présent document spécifie une méthode de référence pour la détermination de l'indice d'iode (communément appelé II dans le secteur industriel) des huiles et graisses d'origines animale et végétale, désignées ci-après par le terme corps gras.
L'Annexe B décrit une méthode de calcul de l'II à partir de données relatives à la composition en acides gras. Cette méthode ne s'applique pas aux huiles de poisson. Par ailleurs, les huiles végétales issues de pression à froid, brutes et non raffinées ainsi que les huiles (partiellement) hydrogénées peuvent donner des résultats différents selon les deux méthodes. L'II calculé est affecté par les impuretés et les produits de dégradation thermique.
NOTE La méthode en Annexe B est fondée sur la méthode officielle Cd 1c-85 de l'AOCS[10].

Rastlinske in živalske maščobe ter olja - Določanje jodnega števila (ISO 3961:2024)

General Information

Status
Published
Public Enquiry End Date
30-Oct-2024
Publication Date
21-Jan-2025
Current Stage
6060 - National Implementation/Publication (Adopted Project)
Start Date
20-Jan-2025
Due Date
27-Mar-2025
Completion Date
22-Jan-2025

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SLOVENSKI STANDARD
01-marec-2025
Nadomešča:
SIST EN ISO 3961:2018
Rastlinske in živalske maščobe ter olja - Določanje jodnega števila (ISO 3961:2024)
Animal and vegetable fats and oils - Determination of iodine value (ISO 3961:2024)
Tierische und pflanzliche Fette und Öle - Bestimmung der Iodzahl (ISO 3961:2024)
Corps gras d'origines animale et végétale - Détermination de l'indice d'iode (ISO
3961:2024)
Ta slovenski standard je istoveten z: EN ISO 3961:2025
ICS:
67.200.10 Rastlinske in živalske Animal and vegetable fats
maščobe in olja and oils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 3961
EUROPEAN STANDARD
NORME EUROPÉENNE
January 2025
EUROPÄISCHE NORM
ICS 67.200.10 Supersedes EN ISO 3961:2018
English Version
Animal and vegetable fats and oils - Determination of
iodine value (ISO 3961:2024)
Corps gras d'origines animale et végétale - Tierische und pflanzliche Fette und Öle - Bestimmung
Détermination de l'indice d'iode (ISO 3961:2024) der Iodzahl (ISO 3961:2024)
This European Standard was approved by CEN on 19 December 2024.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2025 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 3961:2025 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 3961:2025) has been prepared by Technical Committee ISO/TC 34 "Food
products" in collaboration with Technical Committee CEN/TC 307 “Oilseeds, vegetable and animal fats
and oils and their by-products - Methods of sampling and analysis” the secretariat of which is held by
AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by July 2025, and conflicting national standards shall be
withdrawn at the latest by July 2025.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 3961:2018.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
United Kingdom.
Endorsement notice
The text of ISO 3961:2024 has been approved by CEN as EN ISO 3961:2025 without any modification.

International
Standard
ISO 3961
Seventh edition
Animal and vegetable fats and oils —
2024-12
Determination of iodine value
Corps gras d'origines animale et végétale — Détermination de
l'indice d'iode
Reference number
ISO 3961:2024(en) © ISO 2024
ISO 3961:2024(en)
© ISO 2024
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii
ISO 3961:2024(en)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 2
6 Apparatus . 2
7 Sampling . 2
8 Preparation of the test sample and test portion . 3
9 Procedure . 3
10 Calculation . 4
11 Precision . 4
11.1 Interlaboratory test . .4
11.2 Repeatability limit, r .4
11.3 Reproducibility limit, R .4
12 Test report . 5
Annex A (informative) Interlaboratory test . 6
Annex B (informative) Calculated iodine value for non-fish oils . 8
Bibliography .11

iii
ISO 3961:2024(en)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
ISO technical committees. Each member body interested in a subject for which a technical committee
has been established has the right to be represented on that committee. International organizations,
governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely
with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are described
in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the different types
of ISO document should be noted. This document was drafted in accordance with the editorial rules of the
ISO/IEC Directives, Part 2 (see www.iso.org/directives).
ISO draws attention to the possibility that the implementation of this document may involve the use of (a)
patent(s). ISO takes no position concerning the evidence, validity or applicability of any claimed patent
rights in respect thereof. As of the date of publication of this document, ISO had not received notice of (a)
patent(s) which may be required to implement this document. However, implementers are cautioned that
this may not represent the latest information, which may be obtained from the patent database available at
www.iso.org/patents. ISO shall not be held responsible for identifying any or all such patent rights.
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and expressions
related to conformity assessment, as well as information about ISO’s adherence to the World Trade
Organization (WTO) principles in the Technical Barriers to Trade (TBT), see www.iso.org/iso/foreword.html.
This document was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 11,
Animal and vegetable fats and oils, in collaboration with the European Committee for Standardization (CEN)
Technical Committee CEN/TC 307, Oilseeds, vegetable and animal fats and oils and their by-products - Methods
of sampling and analysis, in accordance with the Agreement on technical cooperation between ISO and CEN
(Vienna Agreement).
This seventh edition cancels and replaces the sixth edition (ISO 3961:2018), of which it constitutes a minor
revision.
The changes are as follows:
— entry errors have been corrected in Table 1.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.

iv
International Standard ISO 3961:2024(en)
Animal and vegetable fats and oils — Determination of
iodine value
1 Scope
This document specifies a reference method for the determination of the iodine value (commonly known in
the industry as IV) of animal and vegetable fats and oils, hereinafter referred to as fats.
Annex B describes a method for the calculation of the IV from fatty acid compositional data. This method
is not applicable to fish oils. Furthermore, cold-pressed, crude and unrefined vegetable oils as well as
(partially) hydrogenated oils can give different results by the two methods. The calculated IV is affected by
impurities and thermal degradation products.
[10]
NOTE The method in Annex B is based upon the AOCS Official method Cd 1c-85 .
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content constitutes
requirements of this document. For dated references, only the edition cited applies. For undated references,
the latest edition of the referenced document (including any amendments) applies.
ISO 661, Animal and vegetable fats and oils — Preparation of test sample
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— ISO Online br
...

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