SIST EN ISO 11290-1:1997/A1:2005

Microbiology of food and animal feeding stuffs - Horizontal method for the detection and enumeration of Listeria monocytogenes - Part 1: Detection method - Amendment 1: Modification of the isolation media and the haemolysis test, and inclusion of precision data (ISO 11290-1:1996/AM1:2004)

Mikrobiologie von Lebensmitteln und Futtermitteln - Horizontales Verfahren für den Nachweis und die Zählung von Listeria monocytogenes - Teil 1: Nachweisverfahren - Änderung 1: Veränderung der Selektivmedien und des Hämolyse-Tests und Aufnahme von Präzisionsdaten (ISO 11290-1:1996/Amd.1:2004)

Microbiologie des aliments - Méthode horizontale pour la recherche et le dénombrement de Listeria monocytogenes - Partie 1: Méthode de recherche - Amendement 1: Modification des milieux d'isolement, de la recherche de l'hémolyse et introduction de données de fidélité (ISO 11290-1:1996/AM1:2004)

Mikrobiologija živil in krme - Horizontalna metoda za ugotavljanje prisotnosti in števila Listerie monicitogenes - 1. del: Metoda za ugotavljanje prisotnosti - Dopolnilo 1: Modifikacija medija za izolacijo in preskus hemolize ter vključitev podatkov o natančnosti (ISO 11290-1:1996/AM1:2004)

General Information

• Status: Withdrawn
• Publication Date: 31-Dec-2004
• Withdrawal Date: 08-Aug-2017
• ICS: 07.100.30 - Food microbiology
• Technical Committee: KŽP - Agricultural food products
• Current Stage: 9900 - Withdrawal (Adopted Project)
• Start Date: 09-Aug-2017
• Due Date: 01-Sep-2017
• Completion Date: 09-Aug-2017
• Ref Project: EN ISO 11290-1:1996/A1:2004 - Microbiology of food and animal feeding stuffs - Horizontal method for the detection and enumeration of Listeria monocytogenes - Part 1: Detection method - Amendment 1: Modification of the isolation media and the haemolysis test, and inclusion of precision data (ISO 11290-1:1996/AM1:2004)

Relations

• Replaced By: SIST EN ISO 11290-1:2017 - Microbiology of the food chain - Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. - Part 1: Detection method (ISO 11290-1:2017)
• Effective Date: 01-Sep-2017
• Amends: SIST EN ISO 11290-1:1997 - Microbiology of food and animal feeding stuffs - Horizontal method for the detection and enumeration of Listeria monocytogenes - Part 1: Detection method (ISO 11290-1:1996)
• Effective Date: 22-Dec-2008
• Amends: SIST EN ISO 11290-1:1997 - Microbiology of food and animal feeding stuffs - Horizontal method for the detection and enumeration of Listeria monocytogenes - Part 1: Detection method (ISO 11290-1:1996)
• Effective Date: 08-Jun-2022

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 11290-1:1997/A1:2005
01-januar-2005
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ãWHYLOD/LVWHULHPRQLFLWRJHQHVGHO0HWRGD]DXJRWDYOMDQMHSULVRWQRVWL
'RSROQLOR0RGLILNDFLMDPHGLMD]DL]RODFLMRLQSUHVNXVKHPROL]HWHUYNOMXþLWHY
SRGDWNRYRQDWDQþQRVWL,62$0
Microbiology of food and animal feeding stuffs - Horizontal method for the detection and
enumeration of Listeria monocytogenes - Part 1: Detection method - Amendment 1:
Modification of the isolation media and the haemolysis test, and inclusion of precision
data (ISO 11290-1:1996/AM1:2004)
Mikrobiologie von Lebensmitteln und Futtermitteln - Horizontales Verfahren für den
Nachweis und die Zählung von Listeria monocytogenes - Teil 1: Nachweisverfahren -
Änderung 1: Veränderung der Selektivmedien und des Hämolyse-Tests und Aufnahme
von Präzisionsdaten (ISO 11290-1:1996/Amd.1:2004)
Microbiologie des aliments - Méthode horizontale pour la recherche et le dénombrement
de Listeria monocytogenes - Partie 1: Méthode de recherche - Amendement 1:
Modification des milieux d'isolement, de la recherche de l'hémolyse et introduction de
données de fidélité (ISO 11290-1:1996/AM1:2004)
Ta slovenski standard je istoveten z: EN ISO 11290-1:1996/A1:2004
ICS:
07.100.30 Mikrobiologija živil Food microbiology
SIST EN ISO 11290-1:1997/A1:2005 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 11290-1:1997/A1:2005

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SIST EN ISO 11290-1:1997/A1:2005
EUROPEAN STANDARD
EN ISO 11290-1:1996/A1
NORME EUROPÉENNE
EUROPÄISCHE NORM
October 2004
ICS 07.100.30
English version
Microbiology of food and animal feeding stuffs - Horizontal
method for the detection and enumeration of Listeria
monocytogenes - Part 1: Detection method - Amendment 1:
Modification of the isolation media and the haemolysis test, and
inclusion of precision data (ISO 11290-1:1996/AM1:2004)
Microbiologie des aliments - Méthode horizontale pour la Mikrobiologie von Lebensmitteln und Futtermitteln -
recherche et le dénombrement de Listeria monocytogenes Horizontales Verfahren für den Nachweis und die Zählung
- Partie 1: Méthode de recherche - Amendement 1: von Listeria monocytogenes - Teil 1: Nachweisverfahren -
Modification des milieux d'isolement, de la recherche de Änderung 1: Veränderung der Selektivmedien und des
l'hémolyse et introduction de données de fidélité (ISO Hämolyse-Tests und Aufnahme von Präzisionsdaten (ISO
11290-1:1996/AM1:2004) 11290-1:1996/AM1:2004)
This amendment A1 modifies the European Standard EN ISO 11290-1:1996; it was approved by CEN on 30 September 2004.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for inclusion of this
amendment into the relevant national standard without any alteration. Up-to-date lists and bibliographical references concerning such
national standards may be obtained on application to the Central Secretariat or to any CEN member.
This amendment exists in three official versions (English, French, German). A version in any other language made by translation under the
responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,
Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia,
Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2004 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 11290-1:1996/A1:2004: E
worldwide for CEN national Members.

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SIST EN ISO 11290-1:1997/A1:2005

EN ISO 11290-1:1996/A1:2004 (E)





Foreword


This document (EN ISO 11290-1:1996/A1:2004) has been prepared by Technical Committee
ISO/TC 34 "Agricultural food products" in collaboration with Technical Committee CEN/TC 275
"Food analysis - Horizontal methods", the secretariat of which is held by DIN.

This European Standard shall be given the status of a national standard, either by publication of
an identical text or by endorsement, at the latest by April 2005, and conflicting national
standards shall be withdrawn at the latest by April 2005.

According to the CEN/CENELEC Internal Regulations, the national standards organizations of
the following countries are bound to implement this European Standard: Austria, Belgium,
Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary,
Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
Portugal, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.


Endorsement notice

The text of ISO 11290-1:1996 has been approved by CEN as EN ISO 11290-1:1996/A1:2004
without any modifications.

2

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SIST EN ISO 11290-1:1997/A1:2005

INTERNATIONAL ISO
STANDARD 11290-1
First edition
1996-12-15
AMENDMENT 1
2004-10-15


Microbiology of food and animal feeding
stuffs — Horizontal method for the
detection and enumeration of Listeria
monocytogenes —
Part 1:
Detection method
AMENDMENT 1: Modification of the isolation
media and the haemolysis test, and inclusion
of precision data
Microbiologie des aliments — Méthode horizontale pour la recherche et
le dénombrement de Listeria monocytogenes —
Partie 1: Méthode de recherche
AMENDEMENT 1: Modification des milieux d'isolement, de la
recherche de l'hémolyse et introduction de données de fidélité




Reference number
ISO 11290-1:1996/Amd.1:2004(E)
©
ISO 2004

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SIST EN ISO 11290-1:1997/A1:2005
ISO 11290-1:1996/Amd.1:2004(E)
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ii © ISO 2004 – All rights reserved

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SIST EN ISO 11290-1:1997/A1:2005
ISO 11290-1:1996/Amd.1:2004(E)
Foreword
IISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
ISO technical committees. Each member body interested in a subject for which a technical committee has
been established has the right to be represented on that committee. International organizations, governmental
and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
Amendment 1 to ISO 11290-1:1996 was prepared by Technical Committee ISO/TC 34, Food products,
Subcommittee SC 9, Microbiology.
The isolation media have been modified, as has the haemolysis test. Precision data have been added.

© ISO 2004 – All rights reserved iii

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SIST EN ISO 11290-1:1997/A1:2005

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SIST EN ISO 11290-1:1997/A1:2005
ISO 11290-1:1996/Amd.1:2004(E)

Microbiology of food and animal feeding stuffs — Horizontal
method for the detection and enumeration of Listeria
monocytogenes —
Part 1:
Detection method
AMENDMENT 1: Modification of the isolation media and the
haemolysis test, and inclusion of precision data
Page 2, Subclause 4.3
Replace this subclause by the following:
4.3 Plating out and identification
From the cultures obtained in 4.1 and 4.2, plating out on the two selective solid media:
1)
 Agar Listeria according to Ottaviani and Agosti (ALOA ) (see Reference [1] and B.3);
 any other solid selective medium at the choice of the laboratory complementary to Agar Listeria according
to Ottaviani and Agosti, such as Oxford or PALCAM.
Incubation of the Agar Listeria according to Ottaviani and Agosti at 37 °C ± 1 °C and examination after
24 h ± 3 h, and if necessary after a further 24 h ± 3 h, to check for the presence of characteristic colonies
which are presumed to be L. monocytogenes.
Incubation of the 2nd selective medium at the appropriate temperature and examination after the appropriate
time.

Page 2, Subclauses 5.4.1 and 5.4.2
Replace these subclauses by the following:
1)
5.4.1 First medium: Agar Listeria according to Ottaviani and Agosti (ALOA ) [1]
See B.3.
5.4.2 Second medium
The choice of the second medium is left to the discretion of the testing laboratory. If a commercial medium is
used, the manufacturer's instructions shall be precisely followed regarding its preparation for use.

1) ALOA is an example of a suitable medium available commercially. This information is given for the convenience of
users of this part of ISO 11290 and does not constitute an endorsement by ISO of this product. The use of other media
with the same formulation is allowed.
© ISO 2004 – All rights reserved 1

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SIST EN ISO 11290-1:1997/A1:2005
ISO 11290-1:1996/Amd.1:2004(E)
Page 4, Subclause 9.4
Replace 9.4.1 by the following:
9.4.1 From the primary enrichment culture incubated for 24 h ± 3 h at 30 °C (9.2), take, by means of a loop
or glass rod (6.5), a portion of the culture and inoculate the surface of the first selective plating medium, Agar
Listeria according to Ottaviani and Agosti (5.4.1), so that well-separated colonies are obtained.
Proceed in the same way with the second selective plating-out medium (5.4.2).
Replace 9.4.3 by the following:
9.4.3 Invert the dishes obtained in 9.4.1 and 9.4.2 and place them in an incubator set at 37 °C for Agar
Listeria according to Ottaviani and Agosti (5.4.1) and at the appropriate temperature for the second selective
medium (5.4.2). If a commercial medium is used for the second selective medium, follow the manufacturer's
instructions.
Delete Note 6.
Replace 9.4.4 by the following:
9.4.4 After incubation for 24 h ± 3 h (and for an additional 24 h ± 3 h if the growth is weak or if no colony is
observed after 24 h incubation) for Agar Listeria according to Ottaviani and Agosti or for the appropriate time
(second selective agar), examine the dishes (9.4.3) for the presence of colonies presumed to be Listeria spp.
Replace 9.4.4.1 by the following:
9.4.4.1 Agar Listeria according to Ottaviani and Agosti: Consider as L. monocytogenes the green-blue
colonies surrounded by an opaque halo (typical colonies). If growth is slight, or if no colony is observed, or if
no typical colony is present after 24 h ± 3 h of incubation, re-incubate the plates for a further 24 h ± 3 h.
NOTE 1 Some strains of L. monocytogenes show a very weak halo (even no halo) in cases of stress, in particular acid
stress.
NOTE 2 Some L. monocytogenes are characterized by a slow PIPLC (phosphatidyl inositol phospholipase C) activity.
Such bacteria are detected when the total duration of incubation is more than, for example, 4 days. Some of these strains
could be pathogenic (see Reference [2]).

Replace 9.4.4.2 by the following:
9.4.4.2 Second selective medium: Examine after the appropriate time to check for the presence of
colonies which, from their characteristics, are considered to be presumptive Listeria spp. or monocytogenes,
depending on the type of medium used.

Page 5, Subclause 9.6.1 Haemolysis test
Insert the subclause numbers 9.6.1.1 at the beginning of the text of 9.6.1.
Replace Note 9 by the following subclause:
9.6.1.2 The haemolytic reaction may also be carried out as follows using sheep red blood corpuscles.
Disperse the colony in 150 µl of TSYEB (B.6); incubate at 37 °C for 2 h. Add 150 µl of a suspension of sheep
red blood corpuscles (B.4 of this Amendment). Incubate at 37 °C for 15 min to 60 min, then refrigerate at
3 °C ± 2 °C for approximately 2 h. Examine for haemolytic activity. If the reaction is not definite, leave at
3 °C ± 2 °C for up to 24 h ± 3 h.
2 © ISO 2004 – All rights reserved

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SIST EN ISO 11290-1:1997/A1:2005
ISO 11290-1:1996/Amd.1:2004(E)
Page 7, new Clause 11
Add the following clause after Clause 10:
11 Precision of the method
11.1 General
It is not possible to express the precision of a qualitative method by using the parameters of repeatability and
reproducibility which can be calculated only for quantitative methods. Thus new performance characteristics
have been selected (see Reference [3]). These characteristics are: accuracy (sensitivity for positive samples,
specificity for negative samples), accordance and concordance (see 11.2, 11.3 and 11.4).
The values of these characteristics have been determined by an interlaboratory test on the method organized
within the framework of a European project (see Annex D). Performance characteristics were determined
using three types of food contaminated at various levels and for reference materials. The values derived from
the interlaboratory test may not be applicable to analyte concentration ranges and matrices other than those
given in Annex D.
WARNING — The method which was tested was without this amendment, i.e. the isolation was
performed on PALCAM and Oxford agars. The precision data give some general guidance to the user
on the global performance of the method and these precision data are applicable in particular to this
part of ISO 11290 together with this amendment when the second isolation agar is either Oxford or
PALCAM.
11.2 Accuracy
11.2.1 Definition
Accuracy is the percentage of samples correctly identified.
...