67.190 - Chocolate

This Technical Specification describes a procedure for the qualitative detection of hazelnut (Corylus avellana) in chocolate. DNA is extracted from the chocolate and a specific DNA sequence for hazelnut detected from the gene for corA 1 [4], [5].

This Technical Specification describes a procedure for the qualitative detection of peanut (Arachis hypogaea) in chocolate using real-time PCR based on the gene for the peanut allergen Ara h 2 [1], [2].

This Technical Specification describes a procedure for the qualitative detection of peanut (Arachis hypogaea) in chocolate using real-time PCR based on the gene for the peanut allergen Ara h 2 [1], [2].

This Technical Specification describes a procedure for the qualitative detection of hazelnut (Corylus avellana) in chocolate. DNA is extracted from the chocolate and a specific DNA sequence for hazelnut detected from the gene for corA 1 [4], [5].

ISO 11053:2009 specifies a procedure for the detection and quantification of cocoa butter equivalents (CBEs) and milk fat in milk chocolate by triacylglycerol profiling using high-resolution capillary gas-liquid chromatography, and subsequent data evaluation by simple and partial least-squares regression analysis. CBE admixtures can be detected at a minimum level of 0,5 g CBE/100 g milk chocolate and quantified at a level of 5 % mass fraction CBE addition to milk chocolate with a predicted error of 0,7 g CBE/100 g milk chocolate.

This part of ISO 23275 specifies a procedure for the quantification of cocoa butter equivalents (CBEs) in cocoa butter (CB) and plain chocolate by high-resolution capillary gas chromatography (HR-GC) of triacylglycerols, and subsequent data evaluation by partial least-squares regression analysis.

This part of ISO 23275 specifies a procedure for the detection of cocoa butter equivalents (CBEs) in cocoa butter (CB) and plain chocolate by high-resolution capillary gas liquid chromatography (HR-GC) of triacylglycerols and subsequent data evaluation by regression analysis. The method is applicable for the detection of 2 % CBE admixture to cocoa butter, corresponding to about 0,6 % CBE in chocolate (i.e. the assumed fat content of chocolate is 30 %).

ISO 23275-2:2006 specifies a procedure for the quantification of cocoa butter equivalents (CBEs) in cocoa butter and plain chocolate by high-resolution capillary gas chromatography (HR-GC) of triacylglycerols, and subsequent data evaluation by partial least squares regression analysis.

ISO 23275-1:2006 specifies a procedure for the detection of cocoa butter equivalents (CBEs) in cocoa butter and plain chocolate by high-resolution capillary gas liquid chromatography of triacylglycerols and subsequent data evaluation by regression analysis.
The method is applicable for the detection of 2 % CBE admixture to cocoa butter, corresponding to about 0,6 % CBE in chocolate (i.e. the assumed fat content of chocolate is 30 %).

ISO 23275-1:2006 specifies a procedure for the detection of cocoa butter equivalents (CBEs) in cocoa butter and plain chocolate by high-resolution capillary gas liquid chromatography of triacylglycerols and subsequent data evaluation by regression analysis. The method is applicable for the detection of 2 % CBE admixture to cocoa butter, corresponding to about 0,6 % CBE in chocolate (i.e. the assumed fat content of chocolate is 30 %).

ISO 23275-2:2006 specifies a procedure for the quantification of cocoa butter equivalents (CBEs) in cocoa butter and plain chocolate by high-resolution capillary gas chromatography (HR-GC) of triacylglycerols, and subsequent data evaluation by partial least squares regression analysis.

This method describes a procedure for the qualitative detection of hazelnut (Corylus avellana) in
chocolate. DNA is extracted from the chocolate and a specific DNA sequence for hazelnut
detected from the gene for corA 1.

This method describes a procedure for the qualitative detection of peanut (Arachis hypogaea) in
chocolate using real-time PCR based on the gene for the peanut allergen Ara h 2 [4, 5].

This method describes a procedure for the qualitative detection of hazelnut (Corylus avellana) in
chocolate. DNA is extracted from the chocolate and a specific DNA sequence for hazelnut
detected from the gene for corA 1.

This method describes a procedure for the qualitative detection of peanut (Arachis hypogaea) in
chocolate using real-time PCR based on the gene for the peanut allergen Ara h 2 [4, 5].