SIST EN 17717:2025
(Main)Plant biostimulants - Detection of Salmonella spp.
Plant biostimulants - Detection of Salmonella spp.
This European Standard establishes the methodology for the determination of Salmonella spp. in plant biostimulant products in accordance to the future Regulation of EU fertilising products.
Pflanzen-Biostimulanzien - Nachweis von Salmonella spp.
Dieses Dokument beschreibt ein Verfahren zum Nachweis von Salmonella spp. in Biostimulanzien der folgenden Produktfunktionskategorien (PFCs, en: Product Function Categories) und Komponentenmaterial¬kategorien (CMC, en: Component Material Category) von EU Düngeprodukten, wie in der Verordnung (EU) 2019/1009 des Europäischen Parlaments und des Rates [1] beschrieben:
PFC 6(A): Mikrobielles Pflanzen-Biostimulans;
PFC 6(B): Nicht-mikrobielles Pflanzen-Biostimulans;
CMC 7: Mikroorganismen.
Es erfordert drei aufeinanderfolgende Schritte: eine selektive Anreicherung, eine Isolierung auf einem chromogenen Agar und bei positivem Befund eine Bestätigung mit einer serologischen Untersuchung (und, falls erforderlich, einem Selektivmedium).
Biostimulants des végétaux - Recherche des Salmonella spp.
Le présent document décrit une méthode pour la recherche des Salmonella spp. dans les biostimulants des catégories fonctionnelles de produits (PFC) et de la catégorie de matières constitutives (CMC) des fertilisants UE décrites dans le Règlement (EU) 2019/1009 du Parlement européen et du Conseil [1] :
- PFC 6(A) : Biostimulant microbien des végétaux ;
- PFC 6(B) : Biostimulant non microbien des végétaux ;
- CMC 7 : Microorganismes.
Il nécessite trois phases successives : un enrichissement sélectif, un isolement sur une gélose chromogène, et, en cas de résultat positif, une confirmation par un essai sérologique (et, si nécessaire, un milieu sélectif).
Rastlinski biostimulanti - Ugotavljanje prisotnosti salmonele (Salmonella spp.)
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-februar-2025
Nadomešča:
SIST-TS CEN/TS 17717:2023
Rastlinski biostimulanti - Ugotavljanje prisotnosti salmonele (Salmonella spp.)
Plant biostimulants - Detection of Salmonella spp.
Pflanzen-Biostimulanzien - Nachweis von Salmonella spp.
Biostimulants des végétaux - Recherche des Salmonella spp.
Ta slovenski standard je istoveten z: EN 17717:2024
ICS:
65.080 Gnojila Fertilizers
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN 17717
EUROPEAN STANDARD
NORME EUROPÉENNE
November 2024
EUROPÄISCHE NORM
ICS 65.080 Supersedes CEN/TS 17717:2022
English Version
Plant biostimulants - Detection of Salmonella spp.
Biostimulants des végétaux - Recherche des Pflanzen-Biostimulanzien - Nachweis von
Salmonella spp. Salmonella spp.
This European Standard was approved by CEN on 26 August 2024.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATIO N
E U R OP ÄI S C HES KOMITEE FÜR NORMUN G
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2024 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 17717:2024 E
worldwide for CEN national Members.
Contents Page
European foreword . 4
Introduction . 5
1 Scope . 6
2 Normative references . 6
3 Terms and definitions . 6
4 Principle . 7
4.1 General . 7
4.2 Enrichment in selective liquid medium . 7
4.3 Plating out on selective solid media . 7
4.4 Confirmation . 7
5 Culture media, reagents, antisera . 8
5.1 General . 8
5.2 Isolation chromogenic agar . 8
5.3 Non-selective agar . 8
5.4 Confirmation selective agar . 8
6 Equipment and consumables . 8
7 Sampling . 9
8 Preparation of test sample . 9
9 Procedure . 9
9.1 Test portion and initial suspension . 9
9.2 Selective enrichment . 10
9.3 Isolation . 10
9.4 Confirmation . 10
10 Expression of results . 12
11 Performance characteristics of the method . 12
11.1 Interlaboratory studies. 12
11.2 Sensitivity . 12
11.3 Specificity . 13
11.4 Positive predictive value (PPV) . 13
11.5 Negative predictive value (NPV) . 13
12 Test report . 13
Annex A (normative) Diagram of the procedures . 14
Annex B (normative) Culture media and reagents . 15
Annex C (informative) Examples of selective plating-out media . 19
Annex D (informative) Method validation study and performance characteristics . 21
Annex ZA (informative) Relationship of this European Standard and the essential
requirements of Regulation (EU) 2019/1009 making available on the market of EU
fertilising products aimed to be covered . 24
Bibliography . 25
European foreword
This document (EN 17717:2024) has been prepared by Technical Committee CEN/TC 455 “Plant
Biostimulants”, the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of
an identical text or by endorsement, at the latest by May 2025, and conflicting national standards
shall be withdrawn at the latest by May 2025.
Attention is drawn to the possibility that some of the elements of this document may be the subject
of patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes CEN/TS 17717:2022.
CEN/TS 17717:2022:
— the European foreword and introduction have been updated;
— in Table 1, errors in the interpretation of the serological reactions have been corrected;
— in Annex B, the layout of Table B.1 has been improved;
— in Annex C, the footnotes have been revised;
— Annex D (method validation study) has been added;
— Annex ZA has been added.
This document has been prepared under a standardization request addressed to CEN by the
European Commission. The Standing Committee of the EFTA States subsequently approves these
requests for its Member States.
For the relationship with EU Legislation, see informative Annex ZA, which is an integral part of
this document.
Any feedback and questions on this document should be directed to the users’ national standards
body. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organisations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary,
Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden,
Switzerland, Türkiye and the United Kingdom.
Introduction
The European Committee for Standardization (CEN) was requested by the European Commission
(EC) to draft European Standards or European Standardization deliverables to support the
implementation of Regulation (EU) 2019/1009 of 5 June 2019 [1] laying down rules on the
making available on the market of EU fertilising products (“FPR” or “Fertilising Products
Regulation”).
This standardization request, presented as SR M/564 and relevant amendments, also contributes
to the Communication on “Innovating for Sustainable Growth: A Bio economy for Europe”. The
interest in plant biostimulants has increased significantly in Europe as a valuable tool to use in
agriculture. Standardization was identified as having an important role in order to promote the
use of biostimulants. The work of CEN/TC 455 seeks to improve the reliability of the supply chain,
thereby improving the confidence of farmers, industry, and consumers in biostimulants, and will
promote and support commercialisation of the European biostimulant industry.
WARNING — Persons using this document should be familiar with normal laboratory practice.
This document does not purport to address all of the safety problems, if any, associated with its
use. It is the responsibility of the user to establish appropriate safety and health practices and to
ensure compliance with any national regulatory conditions.
IMPORTANT — It is absolutely essential that tests conducted in accordance with this document
be carried out by suitably trained staff.
1 Scope
This document specifies a method for the detection of Salmonella spp. in biostimulants.
This document is applicable to all microbial biostimulants in agriculture.
This document is applicable to the blends of fertilizing products where a blend is a mix of at least
two of the following component EU fertilising products categories: Fertilizers, Liming Materials,
Soil Improvers, Growing Media, Plant Biostimulants, and where the following category Plant
Biostimulants is the highest % in the blend by mass or volume, or in the case of liquid form by dry
mass. If Plant Biostimulants is not the highest % in the blend, the European Standard for the
highest % of the blend applies. In case a blend of fertilizing products is composed of components
in equal quantity or in case the component EU fertilising products used for the blend have identical
formulations , the user decides which standard to apply.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies.
For undated references, the latest edition of the referenced document (including any
amendments) applies.
EN 17708:2024, Plant biostimulants — Preparation of sample for microbial analysis
EN 17724:2024, Plant Biostimulants — Terminology
EN ISO 7218:2024, Microbiology of food and animal feeding stuffs — General requirements and
guidance for microbiological examinations (ISO 7218:2024)
EN 17702-1:2024, Plant biostimulants — Sampling and sample preparation — Part 1: Sampling
EN ISO 11133:2014, Microbiology of food, animal feed and water — Preparation, production,
storage and performance testing of culture media (ISO 11133:2014)
3 Terms and definitions
For the purposes of this document, the terms and definitions given in EN 17724:2024 and the
following apply.
ISO and IEC maintain terminology databases for use in standardization at the following addresses:
• ISO Online browsing platform: available at https://www.iso.org/obp
• IEC Electropedia: available at https://www.electropedia.org/
3.1
Salmonella spp.
microorganisms which form typical colonies on solid selective media and which display the
morphological, physiological and biochemical characteristics described when the analysis is
carried out in accordance with this document
An example of such a blend is a product with 2 claimed functions consisting of a non-microbial plant
biostimulant and an organic fertilizer composed of 1 kg/kg of plant biostimulant from seaweed
As amended by EN
...
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