SIST EN ISO 7899-2:2000

Water quality - Detection and enumeration of intestinal enterococci - Part 2: Membrane filtration method (ISO 7899-2:2000)

Migrated from Progress Sheet (TC Comment) (2000-07-10): TC 230 Res. 87: // approval procedure on the revision of ISO 7899-2:1984. ++ N315: New TD (TA/980915)

Wasserbeschaffenheit - Nachweis und Zählung von intestinalen Enterokokken - Teil 2: Membranfiltrationsverfahren (ISO 7899-2:2000)

Dieser Teil der ISO 7899 legt ein Verfahren zum Nachweis und Zählung von intestinalen Enterokokken in Wasser durch Membranfiltration fest. Dieser Teil d der ISO 7899 ist besonders zur Untersuchung von Trinkwasser, Wasser aus Schwimmbädern und anderen desinfizierten oder reinen Wässern bestmmit. Trotzdem kann das Verfahren für alle Arten von Wasser angewandt werden, außer wenn große Mengen von Schwebstoffen oder viele störende Mikroorganismen vorhanden sind. Es eignet sich besonders zur Untersuchung großer Wasservolumina, die nur wenige intestinale Enterokokken enthalten.

Qualité de l'eau - Recherche et dénombrement des entérocoques intestinaux - Partie 2: Méthode par filtration sur membrane (ISO 7899-2:2000)

La présente partie de l'ISO 7899 spécifie une méthode pour la recherche et le dénombrement des entérocoques intestinaux dans l'eau par filtration sur membrane. La présente partie de l'ISO 7899 est spécialement adaptée à l'examen des eaux de boisson, des eaux de piscines, et d'autres eaux désinfectées ou propres. Néanmoins, la méthode peut être appliquée à tous types d'eaux, excepté en présence d'une abondance de matières en suspension ou de germes interférents. Elle est particulièrement adaptée à l'examen de grands volumes d'eau contenant peu d'entérocoques intestinaux.

Kakovost vode - Ugotavljanje prisotnosti in števila intestinalnih enterokokov - 2. del: Metoda membranske filtracije (ISO 7899-2:2000)

Standard SIST EN ISO 7899-2:2000 določa metodo za odkrivanje in določanje števila črevesnih enterokokov v vodi z membransko filtracijo. Standard je posebej namenjen za pregledovanje pitne vode, vode iz bazenov in drugih vrst dezinficirane ali čiste vode. Lahko pa se jo uporabi tudi za vse vrste voda, razen tistih, v katerih je velika prisotnost lebdečih delcev ali mikroorganizmov.  Posebej je standard primeren za pregledovanje velikih količin vode, ki vsebuje malo črevesnih enterokokov.

General Information

• Status: Published
• Publication Date: 30-Nov-2000
• ICS: 07.100.20 - Microbiology of water
• Technical Committee: KAV - Water quality
• Current Stage: 6060 - National Implementation/Publication (Adopted Project)
• Start Date: 01-Dec-2000
• Due Date: 01-Dec-2000
• Completion Date: 01-Dec-2000
• Directive: TP037 - Pravilnik o pitni vodi
• Directive: TP128 - Pravilnik o prvih meritvah in obratovalnem monitoringu odpadnih voda
• Directive: TP147 - Uredba o upravljanju kakovosti kopalnih voda
• Directive: TP213 - Pravilnik o minimalnih higienskih zahtevah, ki jih morajo izpolnjevati kopališča in kopalna voda v bazenih
• Directive: TP262 - Uredba o pitni vodi
• Directive: TRRTP064 - Rules on initial measurements and operational monitoring of wastewater
• Directive: TRRTP178 - Uredba o upravljanju kakovosti kopalnih voda
• Ref Project: EN ISO 7899-2:2000 - Water quality - Detection and enumeration of intestinal enterococci - Part 2: Membrane filtration method (ISO 7899-2:2000)

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 7899-2:2000
01-december-2000
Kakovost vode - Ugotavljanje prisotnosti in števila intestinalnih enterokokov - 2.
del: Metoda membranske filtracije (ISO 7899-2:2000)
Water quality - Detection and enumeration of intestinal enterococci - Part 2: Membrane
filtration method (ISO 7899-2:2000)
Wasserbeschaffenheit - Nachweis und Zählung von intestinalen Enterokokken - Teil 2:
Membranfiltrationsverfahren (ISO 7899-2:2000)
Qualité de l'eau - Recherche et dénombrement des entérocoques intestinaux - Partie 2:
Méthode par filtration sur membrane (ISO 7899-2:2000)
Ta slovenski standard je istoveten z: EN ISO 7899-2:2000
ICS:
07.100.20 Mikrobiologija vode Microbiology of water
SIST EN ISO 7899-2:2000 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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INTERNATIONAL ISO
STANDARD 7899-2
Second edition
2000-04-15
Water quality — Detection and enumeration
of intestinal enterococci —
Part 2:
Membrane filtration method
Qualité de l'eau — Recherche et dénombrement d'entérocoques
intestinaux —
Partie 2: Méthode par filtration sur membrane
Reference number
ISO 7899-2:2000(E)
©
ISO 2000

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ISO 7899-2:2000(E)
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ii © ISO 2000 – All rights reserved

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ISO 7899-2:2000(E)
Contents Page
Foreword.iv
Introduction.v
1 Scope .1
2 Normative references .1
3 Terms and definitions .2
4 Principle.2
5 Apparatus .2
6 Culture media and reagents .3
7 Sampling.4
8 Procedure .4
9 Quality assurance.5
10 Expression of results .5
11 Test report .5
Annex A (informative) Further microbiological information on intestinal enterococci .6
Bibliography.7
© ISO 2000 – All rights reserved iii

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ISO 7899-2:2000(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO
member bodies). The work of preparing International Standards is normally carried out through ISO technical
committees. Each member body interested in a subject for which a technical committee has been established has
the right to be represented on that committee. International organizations, governmental and non-governmental, in
liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical
Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 3.
Draft International Standards adopted by the technical committees are circulated to the member bodies for voting.
Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this part of ISO 7899 may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
International Standard ISO 7899-2 was prepared by Technical Committee ISO/TC 147, Water quality,
Subcommittee SC 4, Microbiological methods.
This second edition cancels and replaces the first edition (ISO 7899-2:1984), which has been technically revised.
ISO 7899 consists of the following parts, under the general title Water quality — Detection and enumeration of
intestinal enterococci:
� Part 1: Miniaturized method (Most Probable Number) for surface and waste water
� Part 2: Membrane filtration method
Annex A of this part of ISO 7899 is for information only.
iv © ISO 2000 – All rights reserved

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ISO 7899-2:2000(E)
Introduction
In this part of ISO 7899 a method is described for the isolation of intestinal enterococci. Enterococcus faecalis , E.
faecium, E. durans and E. hirae can be detected and enumerated with the methods described in this part of
ISO 7899. In addition, other Enterococcus species and some species of the genus Streptococcus (namely S. bovis
and S. equinus ) may occasionally be detected. These Streptococcus species do not survive long in water and are
probably not enumerated quantitatively. For purposes of water examination, enterococci can be regarded as
indicators of faecal pollution. However it should be noted that some enterococci found in water can occasionally
also originate from other habitats.
© ISO 2000 – All rights reserved v

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INTERNATIONAL STANDARD ISO 7899-2:2000(E)
Water quality — Detection and enumeration of intestinal
enterococci —
Part 2:
Membrane filtration method
1 Scope
This part of ISO 7899 specifies a method for the detection and enumeration of intestinal enterococci in water by
membrane filtration. This part of ISO 7899 is especially intended for examination of drinking water, water from
swimming pools and other disinfected or clean waters. Nevertheless, the method can be applied to all types of
water, except when a large amount of suspended matter or many interfering microorganisms are present. It is
particularly suitable for the examination of large volumes of water containing only a few intestinal enterococci.
2 Normative references
The following normative documents contain provisions which, through reference in this text, constitute provisions of
this part of ISO 7899. For dated references, subsequent amendments to, or revisions of, any of these publications
do not apply. However, parties to agreements based on this part of ISO 7899 are encouraged to investigate the
possibility of applying the most recent editions of the normative documents indicated below. For undated
references, the latest edition of the normative document referred to applies. Members of ISO and IEC maintain
registers of currently valid International Standards.
ISO 3696:1987, Water for a
...