Safety of toys - Part 10: Organic chemical compounds - Sample preparation and extraction

This Part 10 of the European Standard EN 71 for safety of toys specifies sample preparation and extraction procedures for establishing the release or content of organic compounds from those toys for which requirements exist in EN 71-9.

Sicherheit von Spielzeug - Teil 10: Organisch-chemische Verbindungen - Probenvorbereitung und Extraktion

Der vorliegende Teil 10 der Europäischen Norm EN 71 zur Sicherheit von Spielzeug legt Verfahren für die
Probenvorbereitung und Extraktion fest, um die Freisetzung und den Gehalt von organischen Verbindungen
derjenigen Spielzeuge zu bestimmen, für die in der EN 71-9 Anforderungen bestehen.

Sécurité des jouets - Partie 10: Composés organiques chimiques - Préparation et extraction des échantillons

La présente partie 10 de la Norme européenne EN 71 relative a la sécurité des jouets spécifie les modes opératoires de préparation et d'extraction des échantillons nécessaires a l'établissement du relargage des composés organiques par les jouets ou a la détermination de la teneur de ces composés dans les jouets concernés par les exigences stipulées dans l’EN 71-9.

Varnost igrač – 10. del: Organske kemijske spojine – Priprava vzorcev in ekstrakcija

General Information

Status
Published
Publication Date
31-Jan-2006
Current Stage
6060 - National Implementation/Publication (Adopted Project)
Start Date
01-Feb-2006
Due Date
01-Feb-2006
Completion Date
01-Feb-2006

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Standards Content (sample)

SLOVENSKI STANDARD
SIST EN 71-10:2006
01-februar-2006
9DUQRVWLJUDþ±GHO2UJDQVNHNHPLMVNHVSRMLQH±3ULSUDYDY]RUFHYLQ
HNVWUDNFLMD
Safety of toys - Part 10: Organic chemical compounds - Sample preparation and
extraction
Sicherheit von Spielzeug - Teil 10: Organisch-chemische Verbindungen -
Probenvorbereitung und Extraktion
Sécurité des jouets - Partie 10: Composés organiques chimiques - Préparation et
extraction des échantillons
Ta slovenski standard je istoveten z: EN 71-10:2005
ICS:
97.200.50 ,JUDþH Toys
SIST EN 71-10:2006 en,fr,de

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN 71-10:2006
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SIST EN 71-10:2006
EUROPEAN STANDARD
EN 71-10
NORME EUROPÉENNE
EUROPÄISCHE NORM
December 2005
ICS 97.200.50
English Version
Safety of toys - Part 10: Organic chemical compounds - Sample
preparation and extraction

Sécurité des jouets - Partie 10: Composés chimiques Sicherheit von Spielzeug - Teil 10: Organisch-chemische

organiques - Procédures de préparation d'échantillon et Verbindungen - Probenvorbereitung und Extraktion

d'extraction
This European Standard was approved by CEN on 6 October 2005.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European

Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national

standards may be obtained on application to the Central Secretariat or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation

under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official

versions.

CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,

Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia,

Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36 B-1050 Brussels

© 2005 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 71-10:2005: E

worldwide for CEN national Members.
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SIST EN 71-10:2006
EN 71-10:2005 (E)
Contents page

Foreword ..........................................................................................................................................................3

Introduction......................................................................................................................................................4

1 Scope ...................................................................................................................................................5

2 Normative references .........................................................................................................................5

3 Terms and definitions.........................................................................................................................5

4 Requirements (see B.6) ......................................................................................................................6

5 Sample preparation.............................................................................................................................9

6 Migration – Sampling and extraction.................................................................................................9

6.1 Simulant (see B.7)...............................................................................................................................9

6.2 Apparatus ............................................................................................................................................9

6.3 Sampling..............................................................................................................................................9

6.4 Extraction (see B.8)...........................................................................................................................10

7 Inhalation – Sampling, extraction and analysis..............................................................................10

7.1 First-action method for solvents......................................................................................................10

7.2 Final-action method for solvents.....................................................................................................10

8 Specific sampling and extraction procedures ................................................................................10

8.1 Textiles – Flame retardants, colourants and primary aromatic amines........................................10

8.2 Leather – Colourants, primary aromatic amines and preservatives .............................................11

8.3 Wood – Colourants, primary aromatic amines and wood preservatives......................................13

8.4 Paper – Colourants and primary aromatic amines.........................................................................14

8.5 Aqueous liquids - Colourants, primary aromatic amines and preservatives ...............................14

8.6 Solid toy materials intended to leave a trace - Colourants and primary aromatic amines..........16

8.7 Modelling clay, play clay and similar - Colourants, primary aromatic amines and

preservatives.....................................................................................................................................16

8.8 Balloon-making compounds – Colourants and primary aromatic amines ...................................17

8.9 Imitation tattoos with adhesive - Colourants, primary aromatic amines and preservatives.......18

Annex A (normative) First-action method for colourants and primary aromatic amines........................20

Annex B (informative) Rationale..................................................................................................................22

Annex ZA (informative) Relationship between this European Standard and the Essential

Requirements of EU Directive 88/378/EEC......................................................................................24

Bibliography...................................................................................................................................................25

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SIST EN 71-10:2006
EN 71-10:2005 (E)
Foreword

This European Standard (EN 71-10:2005) has been prepared by Technical Committee CEN/TC 52 “Safety of

Toys”, the secretariat of which is held by DS.

This European Standard shall be given the status of a national standard, either by publication of an identical

text or by endorsement, at the latest by June 2006, and conflicting national standards shall be withdrawn at

the latest by June 2006.

This European Standard has been prepared under a mandate given to CEN by the European Commission

and the European Free Trade Association, and supports essential requirements of EU Directive(s).

For relationship with EU Directive(s), see informative Annex ZA, which is an integral part of this European

Standard.

This European Standard constitutes part 10 of the European Standard on Safety of Toys.

This part should be read in conjunction with parts 9 and 11.

This European Standard defines the sample preparation and extraction procedures used to address potential

exposure to organic compounds from ingestion, mouthing, inhalation, skin contact with solids and skin and

eye contact with liquids.

According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following

countries are bound to implement this European Standard: Austria, Belgium, Cyprus, Czech Republic,

Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,

Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia, Slovenia, Spain, Sweden, Switzerland

and United Kingdom.
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SIST EN 71-10:2006
EN 71-10:2005 (E)
Introduction
The European Standard EN 71 for safety of toys consists of the following parts:
Part 1: Mechanical and physical properties
Part 2: Flammability
Part 3: Migration of certain elements
Part 4: Experimental sets for chemistry and related activities
Part 5: Chemical toys (sets) other than experimental sets
Part 6: Graphical symbols for age warning labelling
Part 7: Finger paints – Requirements and test methods

Part 8: Swings, slides and similar activity toys for indoor and outdoor family domestic use

Part 9: Organic chemical compounds – Requirements
Part 10: Organic chemical compounds – Sample preparation and extraction
Part 11: Organic chemical compounds – Methods of analysis

The standards EN 71-9, EN 71-10 and EN 71-11 were mandated by the European Commission (M/229) to

address the risks presented by organic compounds in toys by taking into account the potential exposure and

toxicological effects of those substances considered to present the greatest risk to health.

This European Standard specifies the procedures for preparing and extracting samples of toy materials prior

to analysis by the methods described in EN 71-11.

This part should be read in conjunction with EN 71-9, which contains requirements for certain organic

compounds in toys, and EN 71-11, which specifies methods of analysis.

This European Standard takes into account the opinion of the Toxicology Section of the Scientific Advisory

Committee published in 1992 (EUR 13976), which recommended that certain groups of chemical compounds

used in toys and toy materials need to be given special attention. In drafting this European Standard CEN/TC

52 has considered organic chemicals that can be classified within the following groups:

 Solvents
 Preservatives
 Plasticisers (excluding phthalate plasticisers)
 Flame retardants
 Monomers
 Biocides (wood preservatives)
 Processing aids
 Colouring agents

During the development of this European Standard, CEN/TC 52 has considered the requirements set out in

Council Directive 82/711/EEC as amended and its supporting standards.

Phthalate plasticisers were specifically excluded from the scope of mandate M/229.

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SIST EN 71-10:2006
EN 71-10:2005 (E)
1 Scope

This Part 10 of the European Standard EN 71 for safety of toys specifies sample preparation and extraction

procedures for establishing the release or content of organic compounds from those toys for which

requirements exist in EN 71-9.
2 Normative references

The following referenced documents are indispensable for the application of this European Standard. For

dated references, only the edition cited applies. For undated references, the latest edition of the referenced

document (including any amendments) applies.
EN 71-1, Safety of toys − Part 1: Mechanical and physical properties
EN 71-9, Safety of toys − Part 9: Organic chemical compounds – Requirements

EN 71-11:2005, Safety of toys − Part 11: Organic chemical compounds − Methods of analysis

EN 20105-A03, Textiles – Tests for colour fastness – Part A03: Grey scale for assessing staining

ISO 105-F10, Textiles – Tests for colour fastness – Part F10: Specification for adjacent fabric: Multifibre

3 Terms and definitions

For the purposes of this European Standard, the following terms and definitions apply.

3.1 (see B.2)
accessible

contact with the articulated probe when tested in accordance with the ‘accessibility-of-a-part-or-component’

test in EN 71-1
3.2
accessible liquid

liquid in or on a toy or accompanying a toy to which the child is likely to become exposed during normal or

foreseeable use of the toy

NOTE Liquid paints, bubble liquids, ink in pens, liquids provided with toys for squirting are examples of accessible liquids

3.3 (see B.3)
first-action method

method of analysis designed to show the compliance of a toy or toy material with the requirements of EN 71-9

for a particular compound or group of compounds
3.4
final-action method

method of analysis for use when compliance cannot be demonstrated by a first-action method

3.5 (see B.4)
laboratory sample
single toy in the form in which it is marketed or intended to be marketed
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SIST EN 71-10:2006
EN 71-10:2005 (E)
3.6
mouth-actuated toy

toy which relies on an action of the mouth to operate and which therefore is designed to come into contact

with the mouth during play. Inflatable toys are not considered mouth-actuated toys unless they rely on an

action of the mouth after inflation

NOTE Toy whistles, toy imitation novelty teeth are examples of mouth-actuated toys

3.7 (see B.5)
mouthed
licked, sucked and chewed
3.8
paper

material, marketed as either paper or card, with a maximum mass per unit area of 400 g/m

3.9
polymeric

consisting of plastic, synthetic rubber, natural rubber, silicone polymer but not other natural polymers

3.10
resin-bonded wood
wood-based material

NOTE Plywood, particle board, chipboard and medium-density fibreboard (MDF) are examples of resin-bonded wood

3.11
test portion
portion of the laboratory sample prepared for analysis
3.12
textile
woven or knitted fabrics, non-woven fibrous material
NOTE Felt is an example of non-woven fibrous material
3.13
toy material
material from which toys and toy components are made
4 Requirements (see B.6)

Toys, toy components and toy materials specified in columns 1 and 2 of Table 1 shall be sampled and

analysed in accordance with Clause 5 and the clauses of this European Standard specified in columns 3 to 12

of Table 1.

If a clause number relating to a first-action method is given in Table 1 for a particular toy / toy component and

toy material, compliance with EN 71-9 may be shown by the analysis of the toy material by that method alone

for the relevant group of organic compounds. A first-action method shall not be used to show non-compliance

with the requirements of EN 71-9.
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SIST EN 71-10:2006
EN 71-10:2005 (E)
Table 1 – Applicable sampling and preparation clauses
Requirements
SPECIFIC TOY/TOY COMPONENT Toy material

Toys intended to be mouthed by children under 3 years of age POLYMERIC 6 6 6

POLYMERIC 6 6 6
3 Toys, or accessible components of toys, with a mass of 150 g or 8.3.1 &
WOOD
8.3.3
less intended to be played with in the hands by children under 3
8.3.2
years of age
4 8.4.1 &
PAPER
8.4.2
5 8.1.3 &
TEXTILE
8.1.1 8.1.2
8.1.4
Toys and accessible components of toys intended for children under
3 years of age
6 8.2.1 &
LEATHER
8.2.3
8.2.2
7 POLYMERIC 6 6 6
8 8.3.1 &
WOOD 8.3.3
Mouthpiece components of mouth-actuated toys
8.3.2
9 8.4.1 &
PAPER
8.4.2

10 Inflatable toys with a surface greater than 0,5 m when fully inflated POLYMERIC 7.1 7.2

11 POLYMERIC 6 7.1 7.2
12 8.1.3 &
TEXTILE 8.1.2 7.1 7.2
Toys worn over the mouth or nose 8.1.4
13 8.4.1 &
PAPER
8.4.2
Flame
Procedure
retardants
Colourants
First action
and
Primary
aromatic
Final action
amines
Monomers
Procedure
- migration
Solvents
Procedure
- migration
First action
Solvents
- inhalation
Final action
Wood
Procedure
preservatives
Procedure Preservatives
Procedure Plasticisers
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SIST EN 71-10:2006
EN 71-10:2005 (E)
Table 1 (continued)
Requirements
SPECIFIC TOY/TOY COMPONENT Toy material
14 POLYMERIC 7.1 7.2
Toys which the child can enter
15 TEXTILE 7.1 7.2

16 Components of graphic instruments sold as toys or used in toys POLYMERIC 6 6 6

Toys and accessible components of toys for indoor use WOOD 8.3.3
18 WOOD
Toys and accessible components of toys for outdoor use 8.3.3
19 Toys and components of toys which mimic food POLYMERIC 6 6 6
20 Solid toy materials intended to leave a trace ALL 8.6
8.5.1 &
Coloured accessible liquids in toys LIQUID 8.5.3
8.5.2
22 Non-coloured accessible liquids in toys LIQUID 8.5.3
23 Modelling clay, play clay and similar, except those chemical toys 8.7.1 &
ALL 8.7.3
addressed by EN 71-5 8.7.2
8.8.1 &
Balloon-making compounds ALL 7.1 7.2
8.8.2
8.9.1 &
Imitation tattoos with adhesive ALL 6 8.9.3
8.9.2
26 Imitation jewellery POLYMERIC 6 6 6
Excluding polymeric coatings with a thickness of less than 500 µm.
Flame
Procedure
retardants
Colourants
First action
and
Primary
aromatic
Final action
amines
Monomers
Procedure
- migration
Solvents
Procedure
- migration
First action
Solvents
- inhalation
Final action
Wood
Procedure
preservatives
Procedure Preservatives
Procedure Plasticisers
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SIST EN 71-10:2006
EN 71-10:2005 (E)
5 Sample preparation

Test portions shall be representative of the toy material in the laboratory sample. Test portions shall only be

taken from accessible parts of the toy.
6 Migration – Sampling and extraction
6.1 Simulant (see B.7)
Water, deionized, demonstrably free of appropriate analytes.
6.2 Apparatus
6.2.1 Stainless steel tweezers

6.2.2 Extraction bottles, approximate volume 250 ml with flat base, a screw neck and provided with a

PTFE-lined rubber septum.
NOTE Bottles with the following dimensions have been found to be satisfactory
Outside diameter: 70 mm
Total height of bottle: 138 mm
Height from bottom to start of neck: 75 mm
Inside neck opening: 30 mm

The laboratory shall demonstrate that the containers and closures used do not contribute to or adsorb the

substances in question. Glassware and seals on vials and bottles shall be clean, undamaged and free from

defects.

6.2.3 Bottle rotator , capable of holding and rotating the extraction bottles in an end-over-end motion at a

constant speed. The distance from the centre of the rotating axis to the centre of the flask shall be

approximately 150 mm.
6.3 Sampling

If the approximate surface area of the laboratory sample is less than 10 cm , test the sample uncut.

In other cases, select the most appropriate part of the laboratory sample, from which to obtain a test portion of

(10 ± 1) cm , in such a way as to minimize the inaccessible and internal surfaces. Where possible, choose a

test portion from a thin part of the laboratory sample. Remove a disc, or other shaped piece if this reduces the

amount of cut edges, with a surface area of (10 ± 1) cm using a suitable cutting instrument. Measure the

approximate surface area of the test portion taking into account the thickness when this is greater than 1 mm.

The edges of the test portion should be smooth in appearance.

In cases where the preparation of the laboratory sample leads to unrealistic results because of the cutting

process, the whole uncut toy or toy component may be tested using proportionate extraction volumes and

apparatus. However, sample sizes of less than 10 cm shall be extracted using 100 ml of simulant (see B.7).

2 Schott Duran is a supplier of a suitable product available commercially. This information is given for the convenience

of users of this European Standard and does not constitute an endorsement by CEN.

Voor 't Labo, Belgium, is a supplier of a suitable product available commercially. This information is given for the

convenience of users of this European Standard and does not constitute an endorsement by CEN.

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SIST EN 71-10:2006
EN 71-10:2005 (E)
Remove any loose particles from the edges of the test portion.

If the test portion is thin and is likely to stick to the walls of the extraction bottle, pierce a small hole in it and

attach a small metallic object through the hole; a paper clip is often appropriate.

6.4 Extraction (see B.8)

Place the test portion into the extraction bottle using tweezers. Add 100 ml simulant (6.1) at (20 ± 2) °C.

Close the extraction bottle and secure it in the rotator. Rotate the bottle at (60 ± 5) r/min for (60 ± 5) min.

Filter the aqueous migrate in the bottle through a plug of glass wool.

Analyse the aqueous migrate using the appropriate method(s) specified in EN 71-11.

7 Inhalation – Sampling, extraction and analysis
7.1 First-action method for solvents
See A.2 of EN 71-11:2005
7.2 Final-action method for solvents
See A.3 of EN 71-11:2005
8 Specific sampling and extraction procedures
8.1 Textiles – Flame retardants, colourants and primary aromatic amines
8.1.1 Flame retardants

Examine the laboratory sample and determine which textile components are present and accessible, and

have an accessible area of 10 cm or more.

Remove test portions from the textile components having an accessible area greater than 10 cm on the toy.

The test portions may be cut from the laboratory sample with a suitable blade. Cut each test portion such that

no dimension exceeds 3 mm. Store each test portion separately in a suitable container.

Weigh, to the nearest 1 mg, approximately 0,5 g of the test portion into a 20-ml amber glass vial with a PTFE-

liner screw cap. Add 5 ml acetonitrile and place the vial in an ultrasonic bath for 60 min at 40 °C. Filter the

extract, transfer to a vial and crimp.
Analyse the extract in accordance with 5.2 of EN 71-11:2005.
8.1.2 First-action method for colourants and primary aromatic amines

This test, specified in Annex A and based on EN ISO 105-E04, is an assessment of whether any colourants

can be transferred from textile toy materials to the mouth, mucous membranes or skin. If textiles are found

not to be colourfast when tested in accordance with the test procedure described in Annex A, they shall be

tested by the final-action method for colourants (8.1.3) and the final-action method for primary aromatic

amines (8.1.4).
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SIST EN 71-10:2006
EN 71-10:2005 (E)
8.1.3 Final-action method for colourants

Examine the laboratory sample and determine which textile components are present and accessible, and

have an accessible area of 10 cm or more. Different colours of the same textile material shall be treated

separately.

Remove test portions from the textile components having an accessible area greater than 10 cm on the toy.

Components which have stained the multifibre fabric in the first-action method for textiles (8.1.2) shall be

tested if their area is greater than 1 cm .

The test portions may be cut from the laboratory sample with a suitable blade. Cut each test portion such that

no dimension exceeds 3 mm. Store each test portion separately in a suitable container.

Weigh, to the nearest 1 mg, approximately 0,5 g of the test portion into a 40-ml amber glass vial with a PTFE-

liner screw cap. Add 10 ml ethanol and place the vial in an ultrasonic bath for 15 min. Transfer the extract

into a test tube and concentrate to about 1 ml under a stream of air or nitrogen. Filter the extract, transfer to a

2-ml vial and crimp.
Analyse the extract in accordance with 5.3 of EN 71-11:2005.
8.1.4 Final-action method for primary aromatic amines

Obtain test portions of the laboratory sample in the same way as detailed in 8.1.3.

Weigh, to the nearest 1 mg, approximately 1,0 g of the test portion into a 50-ml polypropylene tube. Add

15 ml water (6.1) and agitate on a Vortex mixer for 30 s.
and

Centrifuge the tube at 2 000 g for 15 min. Pour the supernatant liquid onto a porous kieselguhr column

allow it to absorb for 20 min.

Extract the kieselguhr with 2 x 40 ml tert-butyl methyl ether. Combine the eluates in a 100-ml round-bottomed

flask and evaporate at 50 °C to approximately 5 ml using a rotary evaporator.

Transfer the ether extract to a graduated 10-ml test tube and then carefully reduce to 1 ml under a gentle

stream of nitrogen at ambient temperature. Transfer the concentrated extract to a 2-ml vial and crimp.

NOTE 1 It should be avoided evaporating the ether extract to dryness as this has been shown to adversely affect

amine recovery.

NOTE 2 The recovery of amines can be improved by converting them to their hydrochloride salts.

Analyse the extract in accordance with 5.4 of EN 71-11:2005.
8.2 Leather – Colourants, primary aromatic amines and preservatives
8.2.1 Colourants

Examine the laboratory sample and determine which leather components are present and accessible, and

have an accessible area of 10 cm or more. Different coloured leathers shall be treated separately.

SPE column Chromabond XTR, 70 ml, 14,5 g, is an example of a suitable product available commercially. This

information is given for the convenience of users of this European Standard and does not constitute an endorsement

by CEN.
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SIST EN 71-10:2006
EN 71-10:2005 (E)

Remove test portions from the leather components having an accessible area greater than 10 cm on the toy.

The test portions may be cut from the laboratory sample with a suitable blade. Cut each test portion such that

no dimension exceeds 3 mm. Store each test portion separately in a suitable container.

Weigh, to the nearest 1 mg, approximately 0,5 g of the test portion into a 40-ml amber glass vial with a PTFE-

liner screw cap. Add 10 ml ethanol and place the vial in an ultrasonic bath for 15 min. Transfer the extract

into a test tube and concentrate to about 1 ml under a stream of air. Filter the extract, transfer to a 2-ml vial

and crimp.
Analyse the extract in accordance with 5.3 of EN 71-11:2005.

NOTE Leather samples are assumed not to be colourfast, and are therefore not subjected to a first-action method.

8.2.2 Primary aromatic amines

Obtain test portions of the laboratory sample in the same way as detailed in 8.2.1.

Weigh, to the nearest 1 mg, approximately 1,0 g of the test portion into a 50-ml polypropylene tube. Add

15 ml water (6.1) and agitate on a Vortex mixer for 30 s.

Centrifuge the tube at 2 000 g for 15 min. Pour the supernatant liquid onto a porous kieselguhr column and

allow it to absorb for 20 min.

Extract the kieselguhr with 2 x 40 ml tert-butyl methyl ether. Combine the eluates in a 100-ml round-bottomed

flask and evaporate at 50 °C to approximately 5 ml using a rotary evaporator.

Transfer the ether extract to a 10-ml test tube and then carefully reduce to 1 ml under a gentle stream of

nitrogen at ambient temperature. Transfer the concentrated extract to a 2-ml vial and crimp.

NOTE 1 It should be avoided evaporating the ether extract to dryness as this has been shown to adversely affect

amine recovery.

NOTE 2 The recovery of amines can be improved by converting them to their hydrochloride salts.

Analyse the extract in accordance with 5.4 of EN 71-11:2005.
8.2.3 Preservatives

Obtain test portions of the laboratory sample in the same way as detailed in 8.2.1.

Weigh, to the nearest 1 mg, approximately 1,0 g of the test portion into a 50-ml polypropylene tube. Add

15 ml water (6.1) and agitate on a Vortex mixer for 30 s. Pour the liquid into a suitable container.

Analyse the extract in accordance with 5.7 of EN 71-11:2005; 5.5.2 of EN 71-11:2005, the method for phenol;

and the method in the relevant EU Directive for the identification and determination of free formaldehyde in

cosmetic products.
NOTE Commission Directive 90/207/EEC refers.

SPE column Chromabond XTR, 70 ml, 14,5 g, is an example of a suitable product available commercially. This

information is given for the convenience of users of this European Standard and does not constitute an endorsement by

CEN.
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SIST EN 71-10:2006
EN 71-10:2005 (E)
8.3 Wood – Colourants, primary aromatic amines and wood preservatives
8.3.1 Colourants

If the thickness of the wooden toy or wooden component is less than 1 cm, obtain a test portion from the wood

representative of the whole accessible surface, such that no dimension of the test portion shall exceed 3 mm.

Obtain at least a 5-g test portion and store in a suitable container.

If the thickness of the wooden toy or wooden component is greater than 1 cm, drill into the wood using a

suitable drill bit to a depth of approximately 1 cm, ensuring that the drillings are distributed evenly over the

accessible surface. Collect at least a 5-g test portion of the shavings produced by the drillings and store in a

suitable container.

Weigh, to the nearest 1 mg, approximately 0,5 g of the test portion into a 40-ml amber glass vial with a PTFE-

liner screw cap. Add 10 ml ethanol and place the vial in an ultrasonic bath for 15 min. Transfer the extract

into a test tube and concentrate to about 1 ml under a stream of air or nitrogen. Filter the extract, transfer to a

2-ml vial and crimp.
Analyse the extract in
...

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