SIST ISO 7305:1997

Milled cereal products -- Determination of fat acidity

Produits de mouture des céréales -- Détermination de l'acidité grasse

Žitni mlevski proizvodi - Določanje maščobne kislosti

General Information

• Status: Withdrawn
• Publication Date: 30-Apr-1997
• Withdrawal Date: 30-Sep-2001
• ICS: 67.060 - Cereals, pulses and derived products
• Technical Committee: KŽP - Agricultural food products
• Current Stage: 9900 - Withdrawal (Adopted Project)
• Start Date: 01-Oct-2001
• Due Date: 01-Oct-2001
• Completion Date: 01-Oct-2001
• Ref Project: ISO 7305:1986 - Milled cereal products — Determination of fat acidity

Relations

• Revised: ISO 7305:1998 - Milled cereal products — Determination of fat acidity
• Effective Date: 12-May-2008
• Revised: SIST ISO 7305:2001 - Milled cereal products -- Determination of fat acidity
• Effective Date: 12-May-2008

Standards Content (Sample)

International Standard 7305
INTERNATIONAL ORGANIZATION FOR STANDARDIZATION*MEIAYHAPOnHAR OPrAHM3Al&lR fl0 CTAHAAPTH3ALWlM.ORGANlSATlON INTERNATIONALE DE NORMALISATION
Milled cereal products - Determination of fat acidity
Produits de mouture des céréales - Détermination de I'acidité grasse
First edition - 1986-03-15
- U DC 664.641 .I : 664.762 : 543.241.5 Ref. No. IS0 7S-1986 (E)
Descriptors : agricultural products, wheat flour, semolina, chemical analysis, determination of content, sulphuric acid.
Price based on 3 pages

---------------------- Page: 1 ----------------------
Foreword
IS0 (the International Organization for Standardization) is a worldwide federation of
national standards bodies (IS0 member bodies). The work of preparing International
Standards is normally carried out through IS0 technical committees. Each member
body interested in a subject for which a technical committee has been established has
the right to be represented on that committee. International organizations, govern-
mental and non-governmental, in liaison with ISO, also take part in the work.
Draft International Standards adopted by the technical committees are circulated to
the member bodies for approval before their acceptance as International Standards by
the IS0 Council. They are approved in accordance with IS0 procedures requiring at
least 75 % approval by the member bodies voting.
International Standard IS0 7305 was prepared by Technical Committee ISO/TC 34,
Agricultural food products.
Users should note that all International Standards uqdergo revision from time to time
and that any reference made herein to any other International Standard implies its
latest edition, unless otherwise stated.
O International Organization for Standardization, 1986 0
Printed in Switzerland

---------------------- Page: 2 ----------------------
INTERNATIONAL STANDARD IS0 7305-1986 (E)
Milled cereal products - Determination of fat acidity
\.,
1 Scope and field of application 5.2 Sodium hydroxide, standard volumetric solution,
c(Na0H) = 0,05 mol/l, in 95 % (V/V) ethanol free from car-
This International Standard specifies a method for the deter-
bonates.
mination of "fat" acidity of the following milled cereal
products : flours and semolinas obtained from wheat and
The exact concentration shall be known and checked im-
durum wheat. It is also applicable to pasta.
mediately prior to each series of determinations of fat acidity.
NOTE - This method appears to be applicable also to flours and Use a solution prepared at least 5 days in advance and stored in
semolinas obtained from maize, but a further interlaboratory test would
a brown glass bottle, fitted with a rubber stopper. The solution
be necessary to confirm this extension of the field of application.
shall be colourless or straw coloured.
NOTE - It is recommended that the ethanol be purified as follows.
2 References
Dissolve 5 to 10 g of sodium hydroxide in 1 litre of ethanol and add
0,5 g of aluminium turnings, boil the mixture under reflux for 1 h, then
distil the ethanol. Dissolve the required quantity of sodium hydroxide
IS0 712, Cereals and cereal products - Determination of
(i.e. to give a concentration of 2 g/l) in the distillate, leave to stand for
moisture content (Routine reference method).
5 days and use the supernatant solution.
IS0 2170, Cereals and pulses - Sampling of milled products.
5.3 Phenolphthalein, indicator solution, 1 g per 100 ml
95 % (V//v) ethanol.
3 Definition
fat acidity : A conventional term for acids, essentially free fatty
acids, extracted under the conditions described in this Inter-
6 Apparatus
national Standard.
6.1 Sieves, of wire gauze, of nominal aperture sizes 1 mm
It is expressed in grams of sulfuric acid per 100 g of dry matter.
(for flour, if necessary), and 160 and 500 pm (for semolina and
pasta).
4 Principle
Centrifuge tubes, of borosilicate or neutral glass, of
6.2
Dissolution of the acids in 95 % (V/V) ethanol at room
capacity 45 ml, hermetically stoppered.
temperature, followed by centrifuging and titration of an
aliquot portion of the supernatant liquid against sodium
hydroxide.
6.3 Centrifuge, capable of a centrifugal acceleration of
2 O00g (generally 5 O00 to 6 O00 r/min).
5 Reagents
6.4 Pipettes, of capacities 10 and 20 ml, class A.
All the reagents used shall be of recognized analytical grade
and the water used shall be distilled water or water of
6.5 Conical flask, of capacity 250 ml.
equivalent purity.
5.1 Ethanol, 95 % (l'/VI.
6.6 Micro-burette, graduated in divisions of 0,Ol ml.
1

---------------------- Page: 3 ----------------------
IS0 7305-1986 E)
8.4 Determination
6.7 Rotary stirrer, capable of 30 to 60 r/min.
8.4.1 Pipette 30 ml of 95 % (V/V) ethanol (5.1) into the
6.8 Balance, accurate to 0,Ol g.
centrifuge tube. Seal the tube hermetically and agitate for 1 h
using the rotary stirrer (6.7) working at a temperature of
20 f 5 OC. Then remove the stopper and centrifuge
...

SLOVENSKI STANDARD
SIST ISO 7305:1997
01-maj-1997
äLWQLPOHYVNLSURL]YRGL'RORþDQMHPDãþREQHNLVORVWL
Milled cereal products -- Determination of fat acidity
Produits de mouture des céréales -- Détermination de l'acidité grasse
Ta slovenski standard je istoveten z: ISO 7305:1986
ICS:
67.060 äLWDVWURþQLFHLQSURL]YRGLL] Cereals, pulses and derived
QMLK products
SIST ISO 7305:1997 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST ISO 7305:1997

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SIST ISO 7305:1997
International Standard 7305
INTERNATIONAL ORGANIZATION FOR STANDARDIZATION*MEIAYHAPOnHAR OPrAHM3Al&lR fl0 CTAHAAPTH3ALWlM.ORGANlSATlON INTERNATIONALE DE NORMALISATION
Milled cereal products - Determination of fat acidity
Produits de mouture des céréales - Détermination de I'acidité grasse
First edition - 1986-03-15
- U DC 664.641 .I : 664.762 : 543.241.5 Ref. No. IS0 7S-1986 (E)
Descriptors : agricultural products, wheat flour, semolina, chemical analysis, determination of content, sulphuric acid.
Price based on 3 pages

---------------------- Page: 3 ----------------------

SIST ISO 7305:1997
Foreword
IS0 (the International Organization for Standardization) is a worldwide federation of
national standards bodies (IS0 member bodies). The work of preparing International
Standards is normally carried out through IS0 technical committees. Each member
body interested in a subject for which a technical committee has been established has
the right to be represented on that committee. International organizations, govern-
mental and non-governmental, in liaison with ISO, also take part in the work.
Draft International Standards adopted by the technical committees are circulated to
the member bodies for approval before their acceptance as International Standards by
the IS0 Council. They are approved in accordance with IS0 procedures requiring at
least 75 % approval by the member bodies voting.
International Standard IS0 7305 was prepared by Technical Committee ISO/TC 34,
Agricultural food products.
Users should note that all International Standards uqdergo revision from time to time
and that any reference made herein to any other International Standard implies its
latest edition, unless otherwise stated.
O International Organization for Standardization, 1986 0
Printed in Switzerland

---------------------- Page: 4 ----------------------

SIST ISO 7305:1997
INTERNATIONAL STANDARD IS0 7305-1986 (E)
Milled cereal products - Determination of fat acidity
\.,
1 Scope and field of application 5.2 Sodium hydroxide, standard volumetric solution,
c(Na0H) = 0,05 mol/l, in 95 % (V/V) ethanol free from car-
This International Standard specifies a method for the deter-
bonates.
mination of "fat" acidity of the following milled cereal
products : flours and semolinas obtained from wheat and
The exact concentration shall be known and checked im-
durum wheat. It is also applicable to pasta.
mediately prior to each series of determinations of fat acidity.
NOTE - This method appears to be applicable also to flours and Use a solution prepared at least 5 days in advance and stored in
semolinas obtained from maize, but a further interlaboratory test would
a brown glass bottle, fitted with a rubber stopper. The solution
be necessary to confirm this extension of the field of application.
shall be colourless or straw coloured.
NOTE - It is recommended that the ethanol be purified as follows.
2 References
Dissolve 5 to 10 g of sodium hydroxide in 1 litre of ethanol and add
0,5 g of aluminium turnings, boil the mixture under reflux for 1 h, then
distil the ethanol. Dissolve the required quantity of sodium hydroxide
IS0 712, Cereals and cereal products - Determination of
(i.e. to give a concentration of 2 g/l) in the distillate, leave to stand for
moisture content (Routine reference method).
5 days and use the supernatant solution.
IS0 2170, Cereals and pulses - Sampling of milled products.
5.3 Phenolphthalein, indicator solution, 1 g per 100 ml
95 % (V//v) ethanol.
3 Definition
fat acidity : A conventional term for acids, essentially free fatty
acids, extracted under the conditions described in this Inter-
6 Apparatus
national Standard.
6.1 Sieves, of wire gauze, of nominal aperture sizes 1 mm
It is expressed in grams of sulfuric acid per 100 g of dry matter.
(for flour, if necessary), and 160 and 500 pm (for semolina and
pasta).
4 Principle
Centrifuge tubes, of borosilicate or neutral glass, of
6.2
Dissolution of the acids in 95 % (V/V) ethanol at room
capacity 45 ml, hermetically stoppered.
temperature, followed by centrifuging and titration of an
aliquot portion of the supernatant liquid against sodium
hydroxide.
6.3 Centrifuge, capable of a centrifugal acceleration of
2 O00g (generally 5 O00 to 6 O00 r/min).
5 Reagents
6.4 Pipettes, of capacities 10 and 20 ml, class A.
All the reagents used shall be of recognized analytical grade
and the water used shall be distilled water or water of
6.5 Conical flask, of capacity 250 ml.
equivalent purity.
5.1 Ethanol, 95 % (l'/VI.
6.6 Micro-burette, graduated in divisions of 0,Ol ml.
1

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SIST ISO 7305:1997
IS0
...

Norme internationale @ 7305
INTERNATIONAL ORGANIZATION FOR STANDARDIZATION.MEMlYHAPO~HAR OPrAHH3AUHR il0 CTAHnAPTH3AUMM.ORGANlSATlON INTERNATIONALE DE NORMALISATION
Produits de mouture des céréales - Détermination
* de l’acidité grasse
Milled cereal products - Determination of fat acidity
Première edition - 1986-03-15
I
CDU 664.641.1 : 664.762 : 543.241.5 Réf. no : IS0 7305-1986 (FI
1%
Descripteurs : produit agricole, farine de blé, semoule, analyse chimique, dosage, acide sulfurique.
1 8

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Avant-propos
L'ISO (Organisation internationale de normalisation) est une fédération mondiale
d'organismes nationaux de normalisation (comités membres de I'ISO). L'élaboration
des Normes internationales est confiée aux comités techniques de I'ISO. Chaque
comité membre intéressé par une étude a le droit de faire partie du comité technique
créé à cet effet. Les organisations internationales, gouvernementales et non gouverne-
mentales, en liaison avec I'ISO participent également aux travaux.
Les projets de Normes internationales adoptés par les comités techniques sont soumis
aux comités membres pour approbation, avant leur acceptation comme Normes inter-
nationales par le Conseil de I'IÇO. Les Normes internationales sont approuvées confor-
mément aux procédures de I'ISO qui requièrent l'approbation de 75 % au moins des
comités membres votants.
La Norme internationale IS0 7305 a été élaborée par le comité technique ISO/TC 34,
Produits agricoles alimentaires.
L'attention des utilisateurs est attirée sur le fait que toutes les Normes internationales
sont de temps en temps soumises à révision et que toute référence faite à une autre
Norme internationale dans le présent document implique qu'il s'agit, sauf indication
contraire, de la dernière édition.
O Organisation internationale de normalisation, 1986 0
Imprimé en Suisse

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NORME INTERNATIONALE IS0 7305-1986 (FI
Produits de mouture des céréales - Détermination
de l'acidité grasse
1 Objet et domaine d'application 5.2 Hydroxyde de sodium, solution titrée à 0,05 mol/l dans
l'éthanol à 95 % (I// V), exempte de carbonates.
e
La présente Norme internationale spécifie une méthode de
Le titre doit être vérifié immédiatement avant chaque série de
détermination de l'acidité dite «grasse» dans les produits de
mouture des céréales suivants: farines et semoules de blé déterminations de l'acidité grasse.
tendre et de blé dur. Elle est également applicable aux pâtes
alimentaires. Utiliser une solution préparée au moins 5 jours à l'avance et
conservée dans un flacon en verre inactinique, muni d'un bou-
chon de caoutchouc. La solution doit être incolore ou jaune
NOTE - Cette méthode paraît également applicable aux farines et
semoules de maïs, mais un essai interlaboratoire complémentaire serait
paille.
nécessaire pour confirmer cette extension du domaine d'application.
NOTE - II est recommandé de purifier l'éthanol de la manière sui-
vante : dissoudre 5 à 10 g d'hydroxyde de sodium dans 1 litre d'éthanol
et ajouter 0,5 g de rognures d'aluminium, porter le mélange à I'ébulli-
2 Références
tion sous reflux pendant 1 h puis distiller l'éthanol. Dissoudre dans le
distillat la quantité requise d'hydroxyde de sodium (soit 2 g/l), laisser
IS0 712, Céréales et produits céréaliers - Détermination de la
reposer 5 jours et utiliser la solution surnageante.
teneur en eau (Méthode de référence pratique).
IS0 2170, Céréales et légumineuses - Échantillonnage des 5.3 Phénolphtaléine, solution à 1 g pour 100 ml dans
produits de mouture. l'éthanol à 95 % ( V/ VI.
3 Définition
6 Appareillage
acidité grasse : Une expression conventionnelle des acides,
6.1 Tamis, en toile métallique, de 1 mm d'ouverture de
essentiellement des acides gras libres, extraits dans les condi-
maille (pour les farines, si nécessaire), et de 160 pm et 500 pm
tions décrites dans la présente Norme internationale.
d'ouverture de maille (pour les semoules et les pâtes alimen-
taires).
Elle est exprimée en grammes d'acide sulfurique pour 100 g de
matière sèche.
6.2 Tubes de centrifugeuse, en verre blanc neutre, de
45 ml, bouchés hermétiquement.
4 Principe
6.3 Centrifugeuse, permettant d'obtenir une accélération
Mise en solution des acides dans l'éthanol à 95 % ( V/ VI à la
OOOg (en général 5 O00 à 6 O00 tr/min).
centrifuge de 2
température du laboratoire, centrifugation et titrage d'une
partie aliquote du surnageant par l'hydroxyde de sodium.
6.4 Pipettes, de 10 ml et 20 ml, classe A.
5 Réactifs
6.5 Fiole conique, de 250 ml.
Tous les réactifs doivent être de qualité analytique reconnue, et
6.6 Micro-burette, graduée en 0,Ol ml.
l'eau utilisée doit être de l'eau distillée ou de pureté équivalente.
Éthanol, à 95 % ( V/ VI. 6.7 Agitateur rotatif, 30 à 60 tr/min.
5.1
1

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IS0 73051986
8.4 Détermination
6.8 Balance, précise à 0,Ol g près.
8.4.1 Verser dans le tube de centrifugeuse, à la pipette, 30 ml
6.9 Broyeur, permettant de broyer sans échauffement sen-
d'éthanol à 95 % (V/V) (5.1). Boucher le tube hermétiquement
sible (cas des semoules et des pâtes alimentaires).
1 h à l'aide de l'agitateur rotatif (6.7) en opé-
et l'agiter durant
rant à une température de 20 f 5 OC. Enlever le b
...