SIST-TS CEN ISO/TS 11133-2:2004/A1:2011
(Amendment)Microbiology of food and animal feeding stuffs - Guidelines on preparation and production of culture media - Part 2: Practical guidelines on performance testing of culture media - Amendment 1: Test microorganisms for commonly used culture media (ISO/TS 11133-2:2003/AMD 1:2011)
Microbiology of food and animal feeding stuffs - Guidelines on preparation and production of culture media - Part 2: Practical guidelines on performance testing of culture media - Amendment 1: Test microorganisms for commonly used culture media (ISO/TS 11133-2:2003/AMD 1:2011)
This Technical Specification sets criteria and methods for the performance testing of culture media. This Technical Specification applies to: - commercial bodies producing and/or distributing ready-to-use or semi-finished reconstituted or dehydrated media to microbiological laboratories; - non-commercial bodies supplying media to third parties; - microbiological laboratories preparing culture media for their own use and evaluating the performance of these media.
Mikrobiologie von Lebensmitteln und Futtermitteln - Anleitung für die Vorbereitung und Herstellung von Nährmedien - Teil 2: Praktische Anleitung zur Leistungsprüfung von Nährmedien - Änderung 1: Prüfkeime für allgemein verwendete Nährmedien (ISO/TS 11133-2:2003/AMD 1:2011)
Microbiologie des aliments - Guide pour la préparation et la production des milieux de culture - Partie 2: Guide général pour les essais de performance des milieux de culture - Amendement 1: Micro-organismes pour essai recommandés pour les milieux de culture les plus usuels (ISO/TS 11133-2:2003/AMD 1:2011)
Mikrobiologija živil in krme - Navodila za pripravo in izdelavo gojišč - 2. del: Praktična navodila za vrednotenje gojišč - Dopolnilo 1: Testni mikroorganizmi za običajno uporabljena gojišča (ISO/TS 11133-2:2003/AMD 1:2011)
Ta tehnična specifikacija postavlja merila in metode za vrednotenje gojišč. Ta tehnična specifikacija velja za: – trgovske organe, ki proizvajajo in/ali distribuirajo že pripravljena ali polgotova rekonstituirana ali dehidrirana gojišča mikrobiološkim laboratorijem; – netrgovske organe, ki dobavljajo gojišča tretjim stranem; – mikrobiološke laboratorije, ki pripravljajo gojišča za lastno uporabo in vrednotijo delovanje teh gojišč.
General Information
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Standards Content (Sample)
SLOVENSKI STANDARD
SIST-TS CEN ISO/TS 11133-2:2004/A1:2011
01-julij-2011
0LNURELRORJLMDåLYLOLQNUPH1DYRGLOD]DSULSUDYRLQL]GHODYRJRMLãþGHO
3UDNWLþQDQDYRGLOD]DYUHGQRWHQMHJRMLãþ'RSROQLOR 7HVWQLPLNURRUJDQL]PL]D
RELþDMQRXSRUDEOMHQDJRMLãþD ,6276 $0'
Microbiology of food and animal feeding stuffs - Guidelines on preparation and
production of culture media - Part 2: Practical guidelines on performance testing of
culture media - Amendment 1: Test microorganisms for commonly used culture media
(ISO/TS 11133-2:2003/AMD 1:2011)
Mikrobiologie von Lebensmitteln und Futtermitteln - Anleitung für die Vorbereitung und
Herstellung von Nährmedien - Teil 2: Praktische Anleitung zur Leistungsprüfung von
Nährmedien - Änderung 1: Prüfkeime für allgemein verwendete Nährmedien (ISO/TS
11133-2:2003/AMD 1:2011)
Microbiologie des aliments - Guide pour la préparation et la production des milieux de
culture - Partie 2: Guide général pour les essais de performance des milieux de culture -
Amendement 1: Micro-organismes pour essai recommandés pour les milieux de culture
les plus usuels (ISO/TS 11133-2:2003/AMD 1:2011)
Ta slovenski standard je istoveten z: CEN ISO/TS 11133-2:2003/A1:2011
ICS:
07.100.30 Mikrobiologija živil Food microbiology
SIST-TS CEN ISO/TS 11133- en,fr,de
2:2004/A1:2011
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
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SIST-TS CEN ISO/TS 11133-2:2004/A1:2011
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SIST-TS CEN ISO/TS 11133-2:2004/A1:2011
TECHNICAL SPECIFICATION
CEN ISO/TS 11133-
2:2003/A1
SPÉCIFICATION TECHNIQUE
TECHNISCHE SPEZIFIKATION
February 2011
ICS 07.100.30
English Version
Microbiology of food and animal feeding stuffs - Guidelines on
preparation and production of culture media - Part 2: Practical
guidelines on performance testing of culture media -
Amendment 1: Test microorganisms for commonly used culture
media (ISO/TS 11133-2:2003/AMD 1:2011)
Microbiologie des aliments - Guide pour la préparation et la Mikrobiologie von Lebensmitteln und Futtermitteln -
production des milieux de culture - Partie 2: Guide général Anleitung für die Vorbereitung und Herstellung von
pour les essais de performance des milieux de culture - Nährmedien - Teil 2: Praktische Anleitung zur
Amendement 1: Micro-organismes pour essai Leistungsprüfung von Nährmedien - Änderung 1: Prüfkeime
recommandés pour les milieux de culture les plus usuels für allgemein verwendete Nährmedien (ISO/TS 11133-
(ISO/TS 11133-2:2003/AMD 1:2011) 2:2003/AMD 1:2011)
This Technical Specification (CEN/TS) was approved by CEN on 13 December 2010 for provisional application.
The period of validity of this CEN/TS is limited initially to three years. After two years the members of CEN will be requested to submit their
comments, particularly on the question whether the CEN/TS can be converted into a European Standard.
CEN members are required to announce the existence of this CEN/TS in the same way as for an EN and to make the CEN/TS available
promptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in parallel to the CEN/TS)
until the final decision about the possible conversion of the CEN/TS into an EN is reached.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2011 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN ISO/TS 11133-2:2003/A1:2011: E
worldwide for CEN national Members.
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SIST-TS CEN ISO/TS 11133-2:2004/A1:2011
CEN ISO/TS 11133-2:2003/A1:2011 (E)
Contents Page
Foreword .3
2
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SIST-TS CEN ISO/TS 11133-2:2004/A1:2011
CEN ISO/TS 11133-2:2003/A1:2011 (E)
Foreword
This document (CEN ISO/TS 11133-2:2003/A1:2011) has been prepared by Technical Committee ISO/TC 34
"Food products" in collaboration with Technical Committee CEN/TC 275 “Food analysis - Horizontal methods”
the secretariat of which is held by DIN.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to announce this Technical Specification: Austria, Belgium, Bulgaria, Croatia, Cyprus,
Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy,
Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia,
Spain, Sweden, Switzerland and the United Kingdom.
Endorsement notice
The text of ISO/TS 11133-2:2003/AMD 1:2011 has been approved by CEN as a CEN ISO/TS 11133-
2:2003/A1:2011 without any modification.
3
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SIST-TS CEN ISO/TS 11133-2:2004/A1:2011
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SIST-TS CEN ISO/TS 11133-2:2004/A1:2011
TECHNICAL ISO/TS
SPECIFICATION 11133-2
First edition
2003-12-15
AMENDMENT 1
2011-02-01
Microbiology of food and animal feeding
stuffs — Guidelines on preparation and
production of culture media —
Part 2:
Practical guidelines on performance
testing of culture media
AMENDMENT 1: Test microorganisms for
commonly used culture media
Microbiologie des aliments — Guide pour la préparation et la production
des milieux de culture —
Partie 2: Guide général pour les essais de performance des milieux de
culture
AMENDEMENT 1: Micro-organismes pour essai recommandés pour les
milieux de culture les plus usuels
Reference number
ISO/TS 11133-2:2003/Amd.1:2011(E)
©
ISO 2011
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SIST-TS CEN ISO/TS 11133-2:2004/A1:2011
ISO/TS 11133-2:2003/Amd.1:2011(E)
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ii © ISO 2011 – All rights reserved
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SIST-TS CEN ISO/TS 11133-2:2004/A1:2011
ISO/TS 11133-2:2003/Amd.1:2011(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
In other circumstances, particularly when there is an urgent market requirement for such documents, a
technical committee may decide to publish other types of document:
— an ISO Publicly Available Specification (ISO/PAS) represents an agreement between technical experts in
an ISO working group and is accepted for publication if it is approved by more than 50 % of the members
of the parent committee casting a vote;
— an ISO Technical Specification (ISO/TS) represents an agreement between the members of a technical
committee and is accepted for publication if it is approved by 2/3 of the members of the committee casting
a vote.
An ISO/PAS or ISO/TS is reviewed after three years in order to decide whether it will be confirmed for a
further three years, revised to become an International Standard, or withdrawn. If the ISO/PAS or ISO/TS is
confirmed, it is reviewed again after a further three years, at which time it must either be transformed into an
International Standard or be withdrawn.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
Amendment 1 to ISO/TS 11133-2:2003 was prepared by Technical Committee ISO/TC 34, Food products,
Subcommittee SC 9, Microbiology.
© ISO 2011 – All rights reserved iii
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SIST-TS CEN ISO/TS 11133-2:2004/A1:2011
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SIST-TS CEN ISO/TS 11133-2:2004/A1:2011
ISO/TS 11133-2:2003/Amd.1:2011(E)
Microbiology of food and animal feeding stuffs — Guidelines on
preparation and production of culture media —
Part 2
Practical guidelines on performance testing of culture media
AMENDMENT 1: Test microorganisms for commonly used culture
media
Page 1, Clause 2
Add the following entries:
ISO 4831, Microbiology of food and animal feeding stuffs — Horizontal method for the detection and
enumeration of coliforms — Most probable number technique
ISO 4832, Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of
coliforms — Colony-count technique
ISO 4833, Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of micro-
organisms — Colony-count technique at 30 °C
ISO 6579, Microbiology of food and animal feeding stuffs — Horizontal method for the detection of Salmonella
spp.
ISO 6887-1, Microbiology of food and animal feeding stuffs — Preparation of test samples, initial suspension
and decimal dilutions for microbiological examination — Part 1: General rules for the preparation of the initial
suspension and decimal dilutions
ISO 6887-2, Microbiology of food and animal feeding stuffs — Preparation of test samples, initial suspension
and decimal dilutions for microbiological examination — Part 2: Specific rules for the preparation of meat and
meat products
ISO 6887-3, Microbiology of food and animal feeding stuffs — Preparation of test samples, initial suspension
and decimal dilutions for microbiological examination — Part 3: Specific rules for the preparation of fish and
fishery products
ISO 6887-4, Microbiology of food and animal feeding stuffs — Preparation of test samples, initial suspension
and decimal dilutions for microbiological examination — Part 4: Specific rules for the preparation of products
other than milk and milk products, meat and meat products, and fish and fishery products
ISO 6887-5, Microbiology of food and animal feeding stuffs — Preparation of test samples, initial suspension
and decimal dilutions for microbiological examination — Part 5: Specific rules for the preparation of milk and
milk products
ISO 6888-1, Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of
coagulase-positive staphylococci (Staphylococcus aureus and other species) — Part 1: Technique using
Baird-Parker agar medium
© ISO 2011 – All rights reserved 1
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SIST-TS CEN ISO/TS 11133-2:2004/A1:2011
ISO/TS 11133-2:2003/Amd.1:2011(E)
ISO 6888-2, Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of
coagulase-positive staphylococci (Staphylococcus aureus and other species) — Part 2: Technique using
rabbit plasma fibrinogen agar medium
ISO 6888-3, Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of
coagulase-positive staphylococci (Staphylococcus aureus and other species) — Part 3: Detection and MPN
technique for low numbers
ISO 7251, Microbiology of food and animal feeding stuffs — Horizontal method for the detection and
enumeration of presumptive Escherichia coli — Most probable number technique
ISO 7932, Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of
presumptive Bacillus cereus — Colony-count technique at 30 °C
ISO 7937, Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of
Clostridium perfringens — Colony-count technique
ISO 10272-1, Microbiology of food and animal feeding stuffs — Horizontal method for detection and
enumeration of Campylobacter spp. — Part 1: Detection method
ISO/TS 10272-2, Microbiology of food and animal feeding stuffs — Horizontal method for detection and
enumeration of Campylobacter spp. — Part 2: Colony count technique
ISO/TS 10272-3, Microbiology of food and animal feeding stuffs — Horizontal method for detection and
enumeration of Campylobacter spp. — Part 3: Semi-quantitative method
ISO 10273, Microbiology of food and animal feeding stuffs — Horizontal method for the detection of
presumptive pathogenic Yersinia enterocolitica
ISO 11290-1, Microbiology of food and animal feeding stuffs — Horizontal method for the detection and
enumeration of Listeria monocytogenes — Part 1: Detection method
ISO 11290-2, Microbiology of food and animal feeding stuffs — Horizontal method for the detection and
enumeration of Listeria monocytogenes — Part 2: Enumeration method
ISO 15213, Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of sulfite-
reducing bacteria growing under anaerobic conditions
ISO 15214, Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of
mesophilic lactic acid bacteria — Colony-count technique at 30° C
ISO 16649 (all parts), Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration
of beta-glucuronidase-positive Escherichia coli
ISO 16654, Microbiology of food and animal feeding stuffs — Horizontal method for the detection of
Escherichia coli O157
ISO 21528-1, Microbiology of food and animal feeding stuffs — Horizontal methods for the detection and
enumeration of Enterobacteriaceae — Part 1: Detection and enumeration by MPN technique with pre-
enrichment
ISO 21528-2, Microbiology of food and animal feeding stuffs — Horizontal methods for the detection and
enumeration of Enterobacteriaceae — Part 2: Colony-count method
2 © ISO 2011 – All rights reserved
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SIST-TS CEN ISO/TS 11133-2:2004/A1:2011
ISO/TS 11133-2:2003/Amd.1:2011(E)
Page 13
Replace the whole of Annex B by the following text.
Annex B
(normative)
Test microorganisms for commonly used culture media
(giving information on the culture medium, culture conditions,
test microorganisms, culture collection number of test organisms
and the expected reactions)
Tables B.1 to B.6 have been established taking into account the control strains used in the European
Pharmacopoeia (EP) and the recommendations from the EP on food microbiology for culture media [Working
Party of the International Committee on Food Microbiology and Hygiene (ICFMH)]. These criteria shall be
included in specific International Standards when prepared or revised in the future. A validated batch of media
is one which has shown satisfactory performance. The strain numbers specified in Tables B.1 to B.7 are those
from the catalogue of universal strain identifiers, containing details of the reference strains represented by
each WDCM number and contact details of the culture collections, compiled by the World Data Centre for
Microorganisms (WDCM) (see Reference [16]). All cited media are described within International Standards.
If strain variability is encountered, investigate the effect of the culture medium (e.g. by obtaining the same
medium from a different manufacturer) and obtain a further reference culture from the culture collection in
which it was originally deposited. Users are requested to feed relevant information on strain variability back to
WG 5, Culture media, of ISO/TC 34/SC 9 through the secretariat of ISO/TC 34/SC 9.
Information on performance testing already published in International Standards has not been included in
Tables B.1 to B.7.
© ISO 2011 – All rights reserved 3
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SIST-TS CEN ISO/TS 11133-2:2004/A1:2011
ISO/TS 11133-2:2003/Amd.1:2011(E)
4 © ISO 2011 – All rights reserved
Table B.1 — Selective m
...
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